Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,868,970	G→A	R184C (CGT→TGT)	pcm ←	protein‑L‑isoaspartate O‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,868,970	0	G	A	100.0%	162.6 / NA	48	R184C (CGT→TGT)	pcm	protein‑L‑isoaspartate O‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/15);  total (33/15)

ACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACGACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGCCATCCATCGCCATGACGGGTTGAAACATTATGTAAATCAAGATTTTTCAGGCGGCGACGTGCCTGCCACTGCAAGCCTTTAATCCGT  >  NC_000913/2868725‑2869208                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               aCGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGc                                                                                                                                                                                                                                            >  1:330829/1‑250 (MQ=255)   ggTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCAcc                                                                                                                                                                                                                                         >  1:117933/1‑251 (MQ=255)            tGGGCTTCCCGCGCTCATTTATCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCaa                                                                                                                                                                                                                                 >  1:256567/1‑250 (MQ=255)                   cccGCGCTCATTTATCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGt                                                                                                                                                                                                                         <  2:117933/251‑1 (MQ=255)                          tCATTTATCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAACAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGCTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCATCCCCGACAACGCACCCGTTTCAAATACTGGTGctcctc                                                                                                                                                                                                                  >  1:281446/1‑251 (MQ=255)                                aTCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCGCCCTACTGAACACTGGAAAAGATGCTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTAc                                                                                                                                                                                                            >  1:274296/1‑251 (MQ=255)                                                 ggTTAATAACCAGTTACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAAATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACAGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCTTTTCAAATACTGGTTCTCCTCCCCTACGGGTAAGACGAGAATcc                                                                                                                                                                                           <  1:77272/251‑1 (MQ=255)                                                  gTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGAGGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAAACCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCGCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATccc                                                                                                                                                                                          <  1:405856/251‑1 (MQ=255)                                                      ataaCCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATGCGGGTGCTCCGCCCCTACGGGTAAGACGAGAATCCCGCCt                                                                                                                                                                                      <  2:80332/251‑1 (MQ=255)                                                          ccAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGt                                                                                                                                                                                  >  1:317027/1‑251 (MQ=255)                                                                      ttACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGAAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCATACGGTTAAGCCGAGAATCCCGCCTTCGCCCAGCTGCGTct                                                                                                                                                                      >  2:40226/1‑250 (MQ=255)                                                                               aaGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAgcgc                                                                                                                                                               >  2:2892/1‑249 (MQ=255)                                                                                   cccccTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTgg                                                                                                                                                         <  2:125978/249‑1 (MQ=255)                                                                                              acacTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCcggcg                                                                                                                                              >  1:529/1‑251 (MQ=255)                                                                                                       aaGATGTTCACGCCACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGGCATTAGCGCAGTTGGAATTTCCGGCGGTGccgccg                                                                                                                                     >  1:129383/1‑251 (MQ=255)                                                                                                                   aTACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGAt                                                                                                                           >  1:201105/1‑249 (MQ=255)                                                                                                                         tGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCTACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCa                                                                                                                    >  1:70932/1‑250 (MQ=255)                                                                                                                              tGCGGCAAAATAACCAGGAAAAATCCAGGTCTTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGgc                                                                                                              >  2:374025/1‑251 (MQ=255)                                                                                                                               gCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTGAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCTATAATAAATTCGCCCCCCCGACAACGCACCCTTTTCAAATACTTGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGATGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGgcg                                                                                                             <  2:357374/251‑1 (MQ=255)                                                                                                                                    aaaaTAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAAAAATGATAGCGTCAAACGGCGCACGt                                                                                                        >  1:338405/1‑251 (MQ=255)                                                                                                                                        tAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGtgcct                                                                                                    >  1:304780/1‑251 (MQ=255)                                                                                                                                          aCCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTCCGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGtgcctgc                                                                                                  <  1:311990/251‑1 (MQ=255)                                                                                                                                             aGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCa                                                                                                >  1:215235/1‑250 (MQ=255)                                                                                                                                                aaaaaTCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCt                                                                                            >  2:161501/1‑251 (MQ=255)                                                                                                                                                     tCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGcc                                                                                        >  1:298653/1‑250 (MQ=255)                                                                                                                                                        aggaaTTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGAGCCTGCCAACCTTGccatcc                                                                                    >  2:152077/5‑251 (MQ=255)                                                                                                                                                             tttCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACACAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGCCATCCATCGc                                                                               >  1:145797/1‑251 (MQ=255)                                                                                                                                                                ccTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGCCATCCATCGCCAt                                                                            >  1:412584/1‑251 (MQ=255)                                                                                                                                                                 cTCACGTTTTAAGCCAGCTCACCCTTCACTAACGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGAGTCAAACGGCGAACGTGCCTGCCCACCTTGCCATCCATCGCCATg                                                                           >  2:94650/1‑251 (MQ=255)                                                                                                                                                                 cTCACGTTTCAAGCAAGCACACCCTTCACTAAAGGAACAAAGCGCCCGGCATCCACGGCATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGATCCTCCCCTACGGGCAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTCGCGCAGTTGGAATTTCCGGCGGTGACGCCGTAACAATAATAGCGTCAAACGGCGCCCGTGCCTGCCAACCTTGCCATCCATCGCCATg                                                                           >  2:277573/1‑251 (MQ=255)                                                                                                                                                                   cACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCTACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTCCGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGCCATCCATCGCCATGAc                                                                         <  1:920/251‑1 (MQ=255)                                                                                                                                                                        tttAAGCCACCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGCCATCCATCGCCATGACGGGt                                                                     <  2:334523/250‑1 (MQ=255)                                                                                                                                                                          tAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGCCATCCATCGCCATGACGGGTTg                                                                   <  1:40226/250‑1 (MQ=255)                                                                                                                                                                           aaGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACGGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGCCATCCATCGCCATGACGGGTTGaa                                                                 <  1:249800/251‑1 (MQ=255)                                                                                                                                                                                    cccccTTCACAAAAGGGACAAAGCGCACGGCCGCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCCCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGTTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGCCAGCCAGCGCCATTACGGGTTGAAACATTATGt                                                        <  2:63542/249‑1 (MQ=255)                                                                                                                                                                                          tCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGCCATCCATCGCCATGACGGGTTGAAACATTATGTAAATc                                                   >  1:173422/1‑250 (MQ=255)                                                                                                                                                                                              tAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCACCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGAAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGCCATCCATCGCCATGACGGGTTGAAACATTATGTAAATCa                                                  >  1:22644/1‑247 (MQ=255)                                                                                                                                                                                                        aaGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGACTCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAACCGGCGCACGTGCCTGCCAACCTTGCCATCCATCGCCATGACGGGTTGAAACATTATGTAAATCAAGATTTTTCAggcg                                    >  2:404767/1‑251 (MQ=255)                                                                                                                                                                                                        aaGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGCCATCCATCGCCATGACGGGTTGAAACATTATGTAAATCAAGATTTTTCAggcg                                    >  1:330553/1‑251 (MQ=255)                                                                                                                                                                                                          gcgcACGGCCTCCACGGTCTCGCTAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTACGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGt                                                                                                                                            >  2:195733/1‑145 (MQ=255)                                                                                                                                                                                                          gcgcACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGt                                                                                                                                            <  1:195733/145‑1 (MQ=255)                                                                                                                                                                                                            gcACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGCCATCCATCGCCATGACGGGTTGAAACATTATGTAAATCAAGATTTTTCAGgcggcg                                 >  1:24481/1‑250 (MQ=255)                                                                                                                                                                                                                   cTCCACGGTATCGATAATAAATTCGCCTCACCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGCCATCCATCGCCATGACGGGTTGAAACATTATGTAAATCAAGATTTTTCAGGCGGCGACGtgcct                         >  1:147953/1‑251 (MQ=255)                                                                                                                                                                                                                     ccACGGTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGCCATCCATCGCCATGACGGGTTGAAACATTATGTAAATCAAGATTTTTCAGGCGGCGACGtgcctgc                       >  2:72089/1‑251 (MQ=255)                                                                                                                                                                                                                          gTATCGATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATGAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGCCATCCATCGCCATGACGGGTTGAAACATTATGTAAATCAAGATTTTTCAGGCGGCGACGTGCCTGCCACTg                  <  1:2892/251‑1 (MQ=255)                                                                                                                                                                                                                               gATAATAAATTCGCCTCCCCGACAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGCCATCCATCGCCATGACGGGTTGAAACATTATGTAAATCAAGATTTTTCAGGCGGCGACGTGCCTGCCACTGCAAGc             >  1:175719/1‑251 (MQ=255)                                                                                                                                                                                                                                         tCGCCTCCCCGCCAACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCCTTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGCCATCCATCGCCATGACGGGTTGAAACATTATGTAAATCAAGATTTTTCAGGCGGCGACGTGCCTGCCACTGCAAGCCTTTACTCCg   >  2:276310/1‑251 (MQ=255)                                                                                                                                                                                                                                          cGCCCCCCCGACACCGCACCCTTTTCCAATACGGGTGCCCCCCCCCCACGGGTTAGACGAGGATCCCGCCTTCGCCCGGCTGCTGCAATAGGTCAGTTGAAAGTGCGGGCGGTGCCGCCGTAACAGTGATAGCGTCAATCGGCGCACGTTCCTGCCACCCTTGCCATCCATCGCCATGACGGGTTGAAACATTATGTAAATCAAAATTTTTCAGGCGGCGACGTGCCTGCCACTGCAAGCCTGTAATCCGt  <  2:115014/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               ACGGTGAATTTTGGGCTTCCCGCGCTCATTTATCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACGACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGACGAGAATCCCGCCTTCGTCCAGCTGCGTCATTAGCGCAGTTGGAATTTCCGGCGGTGCCGCCGTAACAATGATAGCGTCAAACGGCGCACGTGCCTGCCAACCTTGCCATCCATCGCCATGACGGGTTGAAACATTATGTAAATCAAGATTTTTCAGGCGGCGACGTGCCTGCCACTGCAAGCCTTTAATCCGT  >  NC_000913/2868725‑2869208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑