Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,815,493	G→A	S316F (TCT→TTT)	gshA ←	glutamate‑‑cysteine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,815,493	0	G	A	100.0%	155.0 / NA	46	S316F (TCT→TTT)	gshA	glutamate‑‑cysteine ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/25);  total (21/25)

GATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAGGATCACCCGGTTCCAGTTAACGCGTGTACAGGCAAGTTCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGA  >  NC_000913/2815261‑2815738                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      gATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAGGATCACCCGGTTCCAGTTAACGCGTGTACAGGCAAGTTCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGCACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAACGGCGACTcgccgccgc                                                                                                                                                                                                                                     >  2:314502/1‑248 (MQ=255)       cAGCGTCAGACCCGGTTTGCGACCTTCGAGGATCACCCGGTTCCAGTTAACGCGTGTACAGGCAAGTTCGCTACGGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTaa                                                                                                                                                                                                                               <  2:47832/251‑1 (MQ=255)                         gCGACCTTCGAGGATCACCCGGTTCCAGTTAACGCGTGTACAGGCAAGTTCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATc                                                                                                                                                                                                              <  1:288373/250‑1 (MQ=255)                             ccTTCGATGATCACCCGGTTCCAGTTAACGCGTGTACAGGCAAGGTCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCAGCAAAAGGCGACTCGCCGCTGCGGGTAACGCTTTTTGGACGAATCGgcgc                                                                                                                                                                                                         <  1:394405/251‑1 (MQ=255)                                 cGAGGATCACCCGGTTCCAGTTAACGCGTGTACAGGCAAGTTCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTAc                                                                                                                                                                                                     <  1:327495/251‑1 (MQ=255)                                        cACCCGGTTCCAGTTAACGCGTGTACAGGCAAGTTCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGCACAGTTCGt                                                                                                                                                                                              >  1:50051/1‑251 (MQ=255)                                           ccGGTTCCAGTTAACGCGTGTACAGGCAAGTTCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTCAAGTTCGtttt                                                                                                                                                                                           >  2:27169/1‑251 (MQ=255)                                             ggTTCCAGTTAACGCGTGTACAGGCAAGTTCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGCCGAATCGGCGCGTACAGTTCGTTTTc                                                                                                                                                                                          >  2:8101/1‑250 (MQ=255)                                                   aGTTAACGCGTGTACAGGCAAGTTCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGc                                                                                                                                                                                   >  1:121941/1‑251 (MQ=255)                                                         cgcgTGTACAGGCAAGTTCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGCATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACg                                                                                                                                                                             >  2:181971/1‑251 (MQ=255)                                                          gcgTGTACAGGCAAGTTCGCTACTGCTCATTTCCGGTGAATCAGCCAGCGCACACCAGTCCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACGCGCCGCTGCGGGTAACGCGTTTTGTACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGt                                                                                                                                                                            <  1:401788/251‑1 (MQ=255)                                                                aCAGGCAAGTTCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGt                                                                                                                                                                      >  2:77392/1‑251 (MQ=255)                                                                  aGGCAAGTTCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAAACTGCAACACGTTGCTGTTg                                                                                                                                                                    >  1:307039/1‑251 (MQ=255)                                                                         ttCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCAGCTGAGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGATCGTTTTCAATCTGCAACAAGTTGCTGTTGATTTGc                                                                                                                                                              >  1:5658/1‑250 (MQ=255)                                                                           cGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAg                                                                                                                                                            <  1:159732/250‑1 (MQ=255)                                                                                 tGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTg                                                                                                                                                                                                                      >  1:209142/1‑186 (MQ=255)                                                                                 tGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTg                                                                                                                                                                                                                      <  2:209142/186‑1 (MQ=255)                                                                                  gCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCTCCTGCTGGTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTGCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTAc                                                                                                                                                    <  1:245232/251‑1 (MQ=255)                                                                                  gCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCtt                                                                                                                                                      <  1:77392/249‑1 (MQ=255)                                                                                                         gcgcACACCAGACCATAAACAGGGCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTc                                                                                                                             <  1:391977/251‑1 (MQ=255)                                                                                                                      cATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCCCTCTTCGCGTACTCTTCCg                                                                                                                >  1:358948/1‑251 (MQ=255)                                                                                                                         aaaCAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATg                                                                                                             <  2:106592/251‑1 (MQ=255)                                                                                                                                  gAGGAATCGCCCCGGCTGTTCACCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATAGTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACGCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGc                                                                                                                                                           <  2:339985/196‑1 (MQ=255)                                                                                                                                  gAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGc                                                                                                                                                           >  1:339985/1‑196 (MQ=255)                                                                                                                                  gAGGAATCGAACCTGCTGTTCATCTACACCAATCGGCGAGAAAGGGTTGATGTACAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTATCGTACTCTTCCGATGGCGTTTTg                                                                                                     >  2:374622/1‑250 (MQ=255)                                                                                                                                      aaTCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATTTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTGTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGc                                                                                                <  1:258608/251‑1 (MQ=255)                                                                                                                                          gCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGt                                                                                            <  1:380076/251‑1 (MQ=255)                                                                                                                                              ctgctgTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCACCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTa                                                                                         >  1:33834/1‑250 (MQ=255)                                                                                                                                               tgctgTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGCTTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTa                                                                                         >  1:133972/1‑249 (MQ=255)                                                                                                                                                   gTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGccc                                                                                   <  1:264053/251‑1 (MQ=255)                                                                                                                                                           acacCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTa                                                                           <  1:414777/251‑1 (MQ=255)                                                                                                                                                           acacCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGGCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACg                                                                             <  2:161522/249‑1 (MQ=255)                                                                                                                                                            cacCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTTGACGAATCGGCTCGTACAGTTCGTTTTCAATCTTCAACACGTTGCTGTTAATTTGCATCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCCTTTTTATTTCCTGTTTAACACCCGCTACGTAc                                                                          >  2:93240/1‑251 (MQ=255)                                                                                                                                                              ccAATAGGCTAGTACGGGGTGATGTCCAGCGATCGCATTTCAATATATTCAATGCCGCCACGTAAAAGCGCATCAAAAGGCGACTCTCTGATGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTGCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTGTCCCAATACCAATCTTCGCTTACTCTTCCGATGGCGTGTTGATTGCCTGGTTAAGGCCCGCTACGTAcgc                                                                        <  2:367472/251‑3 (MQ=255)                                                                                                                                                                 aTCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGGCGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTa                                                                     <  2:351961/251‑1 (MQ=255)                                                                                                                                                                  actgCGAGAACGGGGTGATGTCCAGCGAACGCACTTCAAAATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGGTTTGGACGAATCTGCGCGTACATGTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTCCTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTAGTaa                                                                    <  2:134523/248‑1 (MQ=255)                                                                                                                                                                          aaCGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGtt                                                            >  1:129643/1‑251 (MQ=255)                                                                                                                                                                           aagtgttgttttCCAGCGAACGCACTTCGATATATTCATTGCCGCCTCGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACTCGTTTTGGACGAATCGGCGTGTTCAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAAGCTTCGCGTACTATTCCGATGGCGTTTTGATTGCCTTTTTAAGGCCCGTTACGTACTCGTAAAGATCGTTg                                                           <  2:44014/240‑1 (MQ=255)                                                                                                                                                                           aCGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTACGGCCCGCTACGTACTCGTAAAGATCGTTg                                                           >  2:85881/1‑251 (MQ=255)                                                                                                                                                                            gggggtGATGGCCAGCGACCGCCCGTCAATATCTTCAATGCCGCCAAGTAACAGCGCATCAAAAGGCTACGCGCCGCGGCGGGTAACGCGTTTTGGACGAATCGGCGCTTACAGGTCGTTTTCAAGCTGCAACACGTTGCTGTTGATTTGCAGCCGCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCGACGTACTCGTAAAGATCGTTGa                                                          <  2:182204/246‑1 (MQ=255)                                                                                                                                                                                   aTGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGGTCGTTGAAGGTAAt                                                   >  1:98207/1‑251 (MQ=255)                                                                                                                                                                                                     tCAATATATTCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCGTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGAGTGCTTTGCg                                  <  1:74356/250‑1 (MQ=255)                                                                                                                                                                                                              tCAATGCCGCCACGTAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTgg                         <  1:314502/250‑1 (MQ=255)                                                                                                                                                                                                                      gccACGTAACAGCACCTCAAAAGGCGACTCGCCGCTGCGGGAAACGCGCTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCCACACGTTGCTGTTGCTTTGCCGCCTCTTACCGCCTTTCTCAATACCCATCTTCGCGTAATCTTCCTATGGCGTTTTGATTGCATGTTTAAGGCCCGCTACGTACTCGTAACGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCg                 >  2:34576/1‑250 (MQ=255)                                                                                                                                                                                                                            tAACAGCGCATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGc          >  1:140671/1‑251 (MQ=255)                                                                                                                                                                                                                                    cATCAAAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGa  <  1:117739/251‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      GATACCCAGCGTCAGACCCGGTTTGCGACCTTCGAGGATCACCCGGTTCCAGTTAACGCGTGTACAGGCAAGTTCGCTACTGCTCATTTCCGGTGCATCAGCCAGCGCACACCAGACCATAAACAGGTCGAGGAATCGCACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGA  >  NC_000913/2815261‑2815738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑