Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	339,935	C→T	A56V (GCC→GTC)	yahH →	putative uncharacterized protein YahH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	339,935	0	C	T	100.0%	49.9 / NA	26	A56V (GCC→GTC)	yahH	putative uncharacterized protein YahH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/16);  total (10/16)

GCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATG  >  NC_000913/339693‑340167                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                         gCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGtcaccatt                                                                                                                                                                                                                                   <  1:367462/250‑3 (MQ=255)     gCTTCGAGAAAGCCATTCTCGGCTGGTGCGAACGTTACGGCGTCGCACGCGCATGTTCAAGCCGGATGCGGCTTGAACGCCTTATTCGTCCTACGAATGGCGCAGGAAGCTTTAGGCCTGGCAAGCGTAGCGCATCAGGCACTAGACACCCATGCCTGATGCGGCGTGAACGCCTTAGCCGGCCTACGAATGGCGCAAGAATCTGTATGCCTGATAAGCGTAGCGCAGCAGGCATTTGTCGCCATTGCCgg                                                                                                                                                                                                                              <  2:170618/251‑1 (MQ=16)          gagaAAGCCATTCTTGGCTGGTGCGAACGGTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTATCACCATTGCCGAATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCCAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAAGCATTTGTCACCATTGCCGGATgcg                                                                                                                                                                                                                         >  1:350876/1‑251 (MQ=17)                    ttCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCCAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTTCCGGATccggcgggaacgc                                                                                                                                                                                                               >  2:270812/1‑244 (MQ=17)                         ggCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCGTTATCCGGCCTACGAATGGCTCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                                                                                          <  1:264324/251‑1 (MQ=255)                            tGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAAAGCCTTATTCGGCCTACGAAAGGCGCAAGGATCTGTAGGCCTGATAAGCGTAGCCCCTCAGGCAGTTGTCACCATTGCCgggatcggagtgaaagccttttccc                                                                                                                                                                                                       >  2:136231/1‑228 (MQ=17)                              gTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATATGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCCTTGCCGGATGCGGCGGGAACGCCCTATCCGGc                                                                                                                                                                                                     >  1:339965/1‑251 (MQ=17)                                   aaCGTTACGGCGTCTGACCTACATGTTCATGCCCGATGCGGCGAGCACGCCTTATCCGGCCTACGAACGGCGCAAGAATCCGTAGGCCCCATAAGCGTAGCGCATCAGGCATTTGTACCCATTGCCTGATGCGGCGTGAACGCCTTATCCGGCCTACGAAAGGCGCACGAATCTGTAAGCCTGATAAGCGTAGCGCATCCGGGCTTTGTCAACCTTGACGGATGCGGCGTGAAAGCCCTATTCGGccatcg                                                                                                                                                                                                >  2:246786/1‑247 (MQ=12)                                      ggtACGGCGTCGGACCTACATGAGCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGt                                                                                                                                                                                             <  1:204960/249‑1 (MQ=14)                                        tACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCATACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGGTGCGGCGTGAACTCCTTATCCCGCCTCCGAGTgg                                                                                                                                                                                           >  2:318302/1‑251 (MQ=14)                                         aCGGCGTCTGGCCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCAGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTgg                                                                                                                                                                                           <  1:64316/250‑1 (MQ=255)                                                   aCCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGGGCGAGAAttt                                                                                                                                                                                >  1:84422/1‑249 (MQ=255)                                                      tACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGGTAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAAACCCTTATCCGGCCTACGGGTGGCGCGGGAAttgt                                                                                                                                                                               >  1:262594/1‑246 (MQ=11)                                                       acagggtCATGCCGGAGGCGGCGTGAACGCCTTATCCGGCCTCCGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAttgta                                                                                                                                                                              <  2:340872/243‑5 (MQ=11)                                                               aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTCCGAGTGGCGCGGGAAT‑TGTATGCCTgcta                                                                                                                                                                    >  1:156402/1‑247 (MQ=11)                                                                            cgttgaCGCCTTATCCGGCCTACGAAGGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCa                                                                                                                                                      <  2:188401/246‑1 (MQ=11)                                                                                                                                                                                              aCGAATGGCACAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCAt                                      <  2:123716/250‑1 (MQ=255)                                                                                                                                                                                               cGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGGAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATaa                                    <  2:20226/251‑1 (MQ=255)                                                                                                                                                                                                  aTGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAAccc                                 <  2:145310/251‑1 (MQ=255)                                                                                                                                                                                                     gcgcAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCAt                              <  2:112758/251‑1 (MQ=255)                                                                                                                                                                                                        cAAGAAGCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAc                           <  2:305253/251‑1 (MQ=14)                                                                                                                                                                                                             aTCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGCGTGGCGCGAGGATTGTCGGCATGCTATGCGTAGCGCAACAGGCAGTCTGGCGAGGGCGATAAACCCATCACCctct                      >  2:232117/1‑251 (MQ=11)                                                                                                                                                                                                                 gTAGGCCTGATAAGCTTAGCGCATCCGGCATTGGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCctct                      <  2:228338/247‑1 (MQ=14)                                                                                                                                                                                                                 gTAGGCCTGATAAGCGTAGCGCATCAGGCCTTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGt                    <  2:138224/249‑1 (MQ=255)                                                                                                                                                                                                                 gTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCTGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATATCCCCATCACCctct                      <  1:330938/247‑1 (MQ=14)                                                                                                                                                                                                                  tAGGCCTGATAAGCGTAGCGCAGCAGGCATTTGTCACCATTGCCGTATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAg                  <  2:357741/250‑1 (MQ=14)                                                                                                                                                                                                                                  aGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATg  <  1:176242/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                         GCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATG  >  NC_000913/339693‑340167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑