| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,814,688 | C→T | A16T (GCG→ACG) | luxS ← | S‑ribosylhomocysteine lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,814,688 | 0 | C | T | 97.2% | 113.3 / ‑4.0 | 37 | A16T (GCG→ACG) | luxS | S‑ribosylhomocysteine lyase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (22/13); total (23/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01 | |||||||||||
CATCTGGCGTACCAATCAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGG > NC_000913/2814445‑2814920 | cATCTGGCGTACCAATCAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGCTAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGACCTGCAGGCGTTTCCATc > 2:121064/1‑251 (MQ=255)cATCTGGCGTACCAATCAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTTCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCAt < 1:33245/250‑1 (MQ=255) cATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAg < 1:303987/250‑1 (MQ=255) aaaacGGTGCGTAAGCCCATTGGCGAGATATCTATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGTGTATGGATCACTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGATGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTCTCCATCCGGGTATGATCGACTGTGAAGCTGTCTa > 2:115950/5‑250 (MQ=255) aaCCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTaac > 1:109447/1‑251 (MQ=255) atatCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAAt < 2:327111/251‑1 (MQ=255) atCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGTGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTATTCACCTCCGGTAAtt < 1:63890/250‑1 (MQ=255) aTAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGTCTGTGAAGCTATCTAACAACGGCCTTTAGCCACCTCCGGTACttttttt > 2:146208/1‑251 (MQ=255) aTAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAAttttttt > 1:14447/1‑251 (MQ=255) aTAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAACAAACAGGCGCTCCCGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGACGCTCTCTAACAACGGCATTTAGCCACCTCCGGTAAttttttt > 2:388494/1‑251 (MQ=255) tctcTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGATGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCACAAGCGCAGATCGAACACGGTGATTGCATCGCCATGCGGGGCGTTCATTGTTTTCGCCACCCGCACTGCAGGCGTTTCCATCCGGGTCTGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTaaa > 1:176770/1‑250 (MQ=255) ttAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAAGTTTCTGAACtctt < 1:130729/249‑1 (MQ=255) taataTGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAAGTTTTTTAAAAATTTTCTGAACtctttc < 2:82743/246‑1 (MQ=255) acgATGGTTACGCATCAAACCAGCACACAGGCGCTACAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACtctttctt > 2:380325/3‑251 (MQ=255) ggTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCGCTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTccc > 1:387569/1‑249 (MQ=255) aaaCCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCAACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTg > 1:388701/1‑249 (MQ=255) aCAGGTGCTCCAGGTTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAg < 1:318834/251‑1 (MQ=255) aCAGGAGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGaa > 2:333813/1‑249 (MQ=255) cAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCAttt < 1:207183/179‑1 (MQ=255) cAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCAttt > 2:207183/1‑179 (MQ=255) ggTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGATCACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGGATGATCGACTGTGAAGCTATCTAACAACGGCATGTAGCCGCCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGtt < 1:380325/251‑1 (MQ=255) aTCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTatcatca < 2:100961/251‑1 (MQ=255) ctTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGCGATTGCGTCGCCATGCGGGGAGATCATCGTTTTCACCACCCGCAATGCAGGCGTTTCCATCCGGGTATGATAGACTGTGACGCTATCTAACAACGGCATTTAACCACCTCCGGTACTTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTg > 1:250647/1‑251 (MQ=255) tCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGCAGCTATCTAACAACGGCATTTAGCCACCTCCGGTACTTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAAt > 2:331296/1‑251 (MQ=255) ttggtCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGaaa < 1:300628/244‑1 (MQ=255) ttcggaaagCAGAAGCGCAGATCGAACCCGGTGACTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCAACCGAACTGCAGGCGTTTCCATCCGGGTATGCTCGAATGTGAAGCTATCTAACAACGGCATTTAGACACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTgg > 2:276980/9‑251 (MQ=255) ggCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGAATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGcc > 2:204683/1‑250 (MQ=255) cACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCCTTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACt > 1:418815/1‑251 (MQ=255) cGCAGATGCGCAGATCGATCGCTGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGGTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCGCCTCCGGTAATTGTTTGAAAAATTTTCTGAACTCTGTCTTGGCAGGCGATTCTGAGTATATGAAATACGGGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACt < 1:390167/249‑1 (MQ=255) aGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCTGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATATTGGCCGCTCACGAGTg < 2:63371/251‑1 (MQ=255) acacGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTTTCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTttttcttt > 2:15044/1‑251 (MQ=255) cacGGGGAGTGCGTCGCCATGCGGGGGGTTAATTGTTTTCGCCACCCGTACTGCAGGCGTTTCCATGCGGGTATGATCACCTGTGAAGATATCTAACCACGGCATTTAGCCAACTACGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGACTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGCTGAAATTTTGGCCACTCACGAGTGGCCTttttatttt > 2:393179/1‑251 (MQ=255) gATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGCAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCACTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTc > 1:229973/1‑250 (MQ=255) ggggTGTTCATTGTTTTCGCCACCCGAACTGCCGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAACTTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGAATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTcccgc > 2:308029/1‑248 (MQ=255) tgtTCATTGTTTTCGCCACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGACCCCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCTAATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCcac > 2:341739/1‑250 (MQ=255) atggTTTTCGCCCCCCGAACTGCAGGCGTTGCCAGCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCAGCTCCGGTAATTTTTTTAAAAATTGTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAACGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCa < 2:332962/248‑1 (MQ=255) ccACCCGAACTGCAGGCGTTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTTTCAGGCGTGTTTTTCCAACCACACCGCATACGGTTCgg > 1:198077/1‑251 (MQ=255) | CATCTGGCGTACCAATCAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGTATGATCGACTGTGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGG > NC_000913/2814445‑2814920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |