Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,749,965	G→A	E398K (GAG→AAG)	yfjD →	UPF0053 family inner membrane protein YfjD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,749,965	0	G	A	100.0%	126.8 / NA	37	E398K (GAG→AAG)	yfjD	UPF0053 family inner membrane protein YfjD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/21);  total (16/21)

GGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCT  >  NC_000913/2749718‑2750208                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    ggggtcGGTTGAAGATATTTTGGAAGAGATGGTCGGCGATTTCACTACGGGTATGTCGCCAACACTTTCCTAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCATCGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGt                                                                                                                                                                                                                                                  <  2:42938/246‑1 (MQ=255)               atatTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCTGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATAtt                                                                                                                                                                                                                                    >  1:115961/1‑251 (MQ=255)                                                 gTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACATACAGGACAATATGATTAAGCAGGTa                                                                                                                                                                                                   >  1:88736/1‑250 (MQ=255)                                                          gCCAACACCTGCCGAAGAGGTCACGCCGCAAACCGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCCCCCGCGTGCGTATTGGCAAGGACGATATCGTTATTACCGCCGGACAGGACAATATGATTAAGCAGGAAAAAGttttac                                                                                                                                                                                         >  2:71882/1‑249 (MQ=255)                                                                                 cgccgcAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAgt                                                                                                                                                                  <  1:346431/251‑1 (MQ=255)                                                                                      cAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGc                                                                                                                                                             >  1:12517/1‑251 (MQ=255)                                                                                          acgacgGTTCGGTGATTATCGATGGCACCGCCAACGTGCGTGACATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCACTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTACTGTGAAACCGCTGCGCGAGAGTGTGGCGGAg                                                                                                                                                         <  2:337019/251‑1 (MQ=255)                                                                                               ggTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTGCAGTACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACg                                                                                                                                                    <  1:165443/251‑1 (MQ=255)                                                                                                       gATTATCGAGGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGc                                                                                                                                            <  2:339659/251‑1 (MQ=255)                                                                                                                     ccGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATg                                                                                                                              <  1:346132/251‑1 (MQ=255)                                                                                                                      cGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTTTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGc                                                                                                                             <  2:110753/251‑1 (MQ=255)                                                                                                                             gTGCGGGAAATCAACAAAGTCGTCAGCTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATGCTTGAGGCACTGGAAGAGATCCCTGGCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTGTCGACGTACAGGACAATGTGATTAAGCAGGTAAAAGCTTTTCCGGTGAAGCCGCTGCGCGGGAGTGTGGCGGAGTAGCGAAAACGGCCCggtgttcggaggcatggcag                                                                                                                      <  2:23845/251‑19 (MQ=255)                                                                                                                                  ggAAATCAACAAAGCATTTAACTGGACTCTACCGGAAGATGTTGCCCGCACGGTTAATAGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCACGTACGATATCGATATTCTCGCCGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGAGAAACCGCTGCGCGAGAGTGTGGCGGAGGAACGAAAACGACCCGGCATTCACATACAGTGCCGTAAat                                                                                                                 >  1:142690/1‑251 (MQ=255)                                                                                                                                       acaacaaAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCCCGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAAttatta                                                                                                            <  2:151882/250‑1 (MQ=255)                                                                                                                                           caaAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGAAGGGCCGTGAATTATTACGa                                                                                                         >  2:67458/1‑250 (MQ=255)                                                                                                                                            aaaGCCTTTAACTGGCAGCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTGTTTCCTGTGAACCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGTCCGTGAATTATTACGaaa                                                                                                       <  1:250266/251‑1 (MQ=255)                                                                                                                                                          gCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCt                                                                                         <  1:105648/251‑1 (MQ=255)                                                                                                                                                                 ccGGAAGATGATGACCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCtt                                                                                  >  2:193064/1‑251 (MQ=255)                                                                                                                                                                   ggAAGATTAGGCCCGCCCGGGTAATGGAGTCATTCTTGCAGCAATTGAAGAGATCCCTGTCCCAGACACCCTCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACTGGACAATATGATTAAGCAGGTAAAATTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGTCCCGGCATTCGCATGCAGTGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTc                                                                                <  2:277649/251‑1 (MQ=255)                                                                                                                                                                                 gCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTAGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCAt                                                                  >  2:147381/1‑251 (MQ=255)                                                                                                                                                                                  cACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCCTTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTAAAGTAACCATc                                                                 >  1:228839/1‑251 (MQ=255)                                                                                                                                                                                     ggTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgcc                                                              >  1:941/1‑251 (MQ=255)                                                                                                                                                                                             gCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAAt                                                      <  1:187385/251‑1 (MQ=255)                                                                                                                                                                                                     cTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGAATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGAc                                              >  1:47462/1‑251 (MQ=255)                                                                                                                                                                                                      ttGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGGGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGAAACCATCGCCGCCCGAATCGTACGAcc                                             >  2:72703/1‑251 (MQ=255)                                                                                                                                                                                                           gCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATAGCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGGAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAATCTTTTGCGTTCGCTACAGTAACCAGCGACGCACGAATCGGACgggtattca                                        <  2:279082/251‑9 (MQ=255)                                                                                                                                                                                                                gtaaGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCAGTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCg                                     <  2:235370/247‑1 (MQ=255)                                                                                                                                                                                                                ggAAGAGATCCCTGTCGCAGGCCCCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGta                                   <  1:34820/251‑1 (MQ=255)                                                                                                                                                                                                                         cccTGTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATAGCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTc                          <  1:310803/251‑1 (MQ=255)                                                                                                                                                                                                                             gTCGCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATAAGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCa                      <  1:72703/251‑1 (MQ=255)                                                                                                                                                                                                                                gCAGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGACAGCAGAAATTAAGCTTTTACTTTCGCTACAGTAACCATCGCCGCACGAATCGTAAAACCATTAAGCGTATAACCCTTCTGCATa                    >  2:306847/1‑250 (MQ=255)                                                                                                                                                                                                                                  aGGCACCCGCGTGCGTATTGGCAAGTACGATATCGGTATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGACTGCAGGGCCGTGAATTATTAAGAAAGCAGAAATTAAGCTTTTGCTTTCCCTACAGTAACCTTCGCCGCACGAATCGTACGACCATTCAGCGTATCACCCTTCTGCAGAATg                 >  2:268215/1‑251 (MQ=255)                                                                                                                                                                                                                                  aGGCACCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATg                 <  1:64895/251‑1 (MQ=255)                                                                                                                                                                                                                                      aCCCGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGccc              >  1:260003/1‑250 (MQ=255)                                                                                                                                                                                                                                       cccGCGTGCGTATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAg            <  2:115961/251‑1 (MQ=255)                                                                                                                                                                                                                                              gcgtATTGGCAAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGtt      <  2:229893/250‑1 (MQ=255)                                                                                                                                                                                                                                                 tATTGGCAAGAACGATATCGATATTATCGACGTACAGGACAATATGCTTAAGCAGGTAAAAGTTTTTACTGTGACACCGCTGCGCGCAAGCGTGGCGGCGTAAAGAACAAGACCAGGCATTAGAATACAGGGCCGTGCTTTATTAAGAAAGCAGAAATTAAGCTTTTGCTTTCGCTAAAGTCACCATCGCCGCACGAAACGTACGCCCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCt  >  2:181319/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    GGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCT  >  NC_000913/2749718‑2750208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑