Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,715,294	A→G	L9L (CTT→CTC)	yfiE ←	putative LysR‑type DNA‑binding transcriptional regulator YfiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,715,294	0	A	G	100.0%	91.7 / NA	29	L9L (CTT→CTC)	yfiE	putative LysR‑type DNA‑binding transcriptional regulator YfiE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/14);  total (15/14)

GTCCGGATCTGACTCCTTTTTGGCAGCCTCACGAAGCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCA  >  NC_000913/2715054‑2715535                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  gTCCGGATCTGACTCCTTTTTGGCAGCCTCACGAAGCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCCCATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAATGAACCCTCTTCCACCACGGTTTTGAGCGtaataa                                                                                                                                                                                                                                         <  1:164789/251‑1 (MQ=255)                   tttGGCAGCCTCACGAAGCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATa                                                                                                                                                                                                                        <  1:141922/250‑1 (MQ=255)                          gCCTCCCGAAGCGTAGCCATCACTCGCGTAAGGTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAGTCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTGTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGTTTAATAAAACTGCGCAGATCCATAACCCCaaa                                                                                                                                                                                                                <  2:51750/251‑1 (MQ=255)                                     cGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGaaaa                                                                                                                                                                                                     <  1:396764/251‑1 (MQ=255)                                              cACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCCAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAACCCTATCGAAAATATCGAATc                                                                                                                                                                                            >  1:307459/1‑251 (MQ=255)                                               aCTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTGTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGGAATAAGACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCt                                                                                                                                                                                           <  1:26434/251‑1 (MQ=255)                                                  cGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCTGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAACCTCCTGCTCAAGCTGCTGAATATGAAAATGCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCGCGCAAAAAGGAACCCTCTTCCACCTCGGTTTTGAGCGGAAGAAAACGGTGCAGATCCATAACCCTAAACCTATCCAAAATATCGAACCTAg                                                                                                                                                                                         <  2:76862/250‑1 (MQ=255)                                                       aaGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAatat                                                                                                                                                                                    >  1:377270/1‑250 (MQ=255)                                                       aaGTTCATAAATGCGAGGCAGCAACTTTTTTACTTCGCGGGTAAGGCACATCAGTCGGCCAATATTCTCAAATAACTGGCCTGAAAACTACTGCTCAAGCTGCTGAATATGACAAGTCACCGTCGATTGTGTACAGCACATTTTTTGCGAAGCTCGCAAACAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAatat                                                                                                                                                                                    >  2:244683/1‑250 (MQ=255)                                                            cATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTAGCGAAAATATCGAATCTAGAATATAAAAAc                                                                                                                                                                              <  2:344629/251‑1 (MQ=255)                                                               aaaTGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGCAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAAcatt                                                                                                                                                                           >  1:212491/1‑251 (MQ=255)                                                                         cagcaACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTa                                                                                                                                                                 <  1:218523/251‑1 (MQ=255)                                                                                     tCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCgtgt                                                                                                                                                     >  1:63610/1‑251 (MQ=255)                                                                                       cTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCg                                                                                                                                                   >  1:137527/1‑251 (MQ=255)                                                                                                                                aactgaactgAAAACTCCTGTTCAAGCTGCTGAATATGAAAAGTCACCTTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGTAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAAGCCTATCGAAAATATCGAATCTAGAATATAAAATCATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACAcc                                                                                                           <  1:59121/250‑1 (MQ=255)                                                                                                                                     gACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCt                                                                                                     >  2:90138/1‑251 (MQ=255)                                                                                                                                            aaCTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCGCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTAGCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTGTAAGt                                                                                              <  2:377270/251‑1 (MQ=255)                                                                                                                                                ccTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCt                                                                                           <  1:216467/250‑1 (MQ=255)                                                                                                                                                   gCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCttttt                                                                                       >  1:296359/1‑251 (MQ=255)                                                                                                                                                   gCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCttttt                                                                                       <  1:170006/251‑1 (MQ=255)                                                                                                                                                      cAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTATGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTAATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAGCAGAGGAGATAACAAGGGAAACCGACCCTTTTAAGTGCTTTTTgg                                                                                     >  1:140166/1‑250 (MQ=255)                                                                                                                                                            gctgAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATGTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATGCATTTTTATAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGGGCTTTTTCGACTTa                                                                                <  2:168759/249‑1 (MQ=255)                                                                                                                                                                         cgTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATACCCCCACACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGGGTCGGGTACTGTCTACCAAAACAGCGGAGATAACAAGTGAAAACGCCCCTTTTAAGTGGTTTTTGGACTTACACCCTAATTACCGc                                                                 >  2:11853/2‑251 (MQ=255)                                                                                                                                                                         aGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAACCCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGc                                                                 >  1:411542/1‑251 (MQ=255)                                                                                                                                                                                                   aTTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACCCCCTGTTAACCGCTATGAAGCCAGGTCCGAAAAATAttt                                       >  1:3976/1‑250 (MQ=255)                                                                                                                                                                                                                        cggAACACTCTTCCACCACGGATTTGAGCGTATTAACACGGCGCAGATCAATACCCCAAAACCTATCGTAAATCTCGCATCTAGCATATAAAAACATTCATTTTTTTAAATGATCCGGGTCGGGTACTATCTTCCACAACAGCGGAGATAACAATTCACAACGACCCTTTTAATTGCTTTTTCGACTTACACCCTGATTACCGCTATGACGCCAGGCCCTAACAATATTCTCGCCCTTAGCTCTGCTAcct                  >  2:398257/2‑249 (MQ=255)                                                                                                                                                                                                                                   tCCACCACGGTTTTGAGCGTAATAAAAAGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGAttt       >  2:212893/1‑251 (MQ=255)                                                                                                                                                                                                                                        cacGTTTTTGAGCGTAATAAAACGGCGCAGATCCATAACCCCAAGCCTATCGAAAATATCGAATCTAGAATATAAAAACATGCATTTTTTTAAAGGTTCCGTGTCGTGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCGTTTTGGACTTACGCCCTGATTACCGCTATGACGCCGGGACCGAAGATTATTCTCGCCCATAGCTCTGCCACGTCGCATGGATGTCGTCa  <  2:336927/251‑1 (MQ=255)                                                                                                                                                                                                                                        cacGGTTTTGAGAGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGCATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGCTTACCGTTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGta   >  2:22858/1‑249 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  GTCCGGATCTGACTCCTTTTTGGCAGCCTCACGAAGCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAACCCTCTTCCACCACGGTTTTAAGCGTAATAAAACGGCGCAGATCCATAACCCCAAACCTATCGAAAATATCGAATCTAGAATATAAAAACATTCATTTTTTTAAATGTTCCGTGTCGGGTACTGTCTACCAAAACAGAGGAGATAACAAGTGACACCGACCCTTTTAAGTGCTTTTTGGACTTACACCCTGATTACCGCTATGACGCCAGGACCGAACAATATTCTCGCCCTTAGCTCTGCTACGTCGCATGGATTTCGTCA  >  NC_000913/2715054‑2715535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑