Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,703,341	G→A	R16C (CGT→TGT)	era ←	30S ribosomal subunit maturation GTPase Era

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,703,341	0	G	A	100.0%	119.8 / NA	38	R16C (CGT→TGT)	era	30S ribosomal subunit maturation GTPase Era
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/24);  total (14/24)

CTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTG  >  NC_000913/2703095‑2703583                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   cTTCAACGACAAAAATCACCAGCTCAACATCGCCAATGGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCcg                                                                                                                                                                                                                                                >  2:347173/1‑251 (MQ=255)    cAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCcgacg                                                                                                                                                                                                                                             <  2:104546/251‑1 (MQ=255)            aaaaTCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTGCTGCCACAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAAt                                                                                                                                                                                                                                     <  2:91869/251‑1 (MQ=255)                                gCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAAACCGCAGTAACTTTTAt                                                                                                                                                                                                                 <  2:125703/251‑1 (MQ=255)                                 ccAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATc                                                                                                                                                                                                                <  1:5928/251‑1 (MQ=255)                                     aagagCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATg                                                                                                                                                                                                            <  2:111011/250‑1 (MQ=255)                                         gctgctaaccgcTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGCTCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCACCAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTc                                                                                                                                                                                                         >  2:45140/9‑250 (MQ=255)                                               cgccgcTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTATCTTTTATCGATGCCCATTcc                                                                                                                                                                                                    <  2:194934/249‑1 (MQ=255)                                                gccgcTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCCCAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGAGATGGAGATTTTCTGACCCAGCATTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCAGAAGTAACTTTTATCGATGCTCATTccggc                                                                                                                                                                                                 >  1:159768/1‑248 (MQ=255)                                                  cgcTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTc                                                                                                                                                                                               >  1:183683/1‑251 (MQ=255)                                                  cgcTTTGTGCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTc                                                                                                                                                                                               <  2:90365/251‑1 (MQ=255)                                                   gcTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATCCCAGCTcc                                                                                                                                                                                              <  1:232963/251‑1 (MQ=255)                                                      ttGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTTCCCTAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAg                                                                                                                                                                                            <  1:79526/250‑1 (MQ=255)                                                              caGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGGTGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTACAGCTCCAGTTTTTTCa                                                                                                                                                                                    >  1:190300/1‑250 (MQ=255)                                                                                       tcCATATGCAGGCCCGGTGTATAGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTc                                                                                                                                                           >  1:293934/1‑250 (MQ=255)                                                                                              gCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTac                                                                                                                                                   <  1:203392/251‑1 (MQ=255)                                                                                                   cccGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGACAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACgg                                                                                                                                              <  1:83784/251‑1 (MQ=255)                                                                                                         gtATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGAGCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTGTGATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGTCAGCCTGCTCAGCCTTACGACGGCTTGaa                                                                                                                                        <  2:174440/251‑1 (MQ=255)                                                                                                              gACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGt                                                                                                                                                                                                                          >  1:342501/1‑164 (MQ=255)                                                                                                              gACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGt                                                                                                                                                                                                                          <  2:342501/164‑1 (MQ=255)                                                                                                                    gATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTGCCAac                                                                                                                             <  1:332427/251‑1 (MQ=255)                                                                                                                        gCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTGCCAaccacc                                                                                                                         >  1:225570/1‑251 (MQ=255)                                                                                                                                               gATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCAGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTg                                                                                                  <  1:231041/251‑1 (MQ=255)                                                                                                                                                                                  tGCGGGAAGTGATGGGGGTTGTTTGCCCCATCAGTTTGGTCAACAATGTGGATTTGCCAACGTTCGGACAGCCGACGATGGCAAGAAATCCGCAGTAACTTTTATCGATGCTGATTCCAGCGCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAGCCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACGTGGCAGTGGATAGTAAATGCCTGGTCGTGCGCTTc                                                                 <  2:47693/249‑1 (MQ=255)                                                                                                                                                                                              tGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCAAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTgg                                                    >  1:176403/1‑250 (MQ=255)                                                                                                                                                                                               ggAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGGTCGCTTCGCCACGTACCTGGAc                                                  >  1:80406/1‑251 (MQ=255)                                                                                                                                                                                                          gCCCCAGCAGTTTGTTCAACAAGGGGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAGAt                                          <  2:183683/248‑1 (MQ=255)                                                                                                                                                                                                            cccAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAGATAAGTc                                     <  2:355473/251‑1 (MQ=255)                                                                                                                                                                                                                 cagTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTCTATCGATGCTCATTCCAGCGCCAGTTTTTTCAACGCCTCTTCGGCGCCAGCCTGCTCAGCCTTTCGACGGCTTGAACCTGTGCCAACCTCCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAGATTCCTGATCGTGCGCTTCGCCACGTACCTGGGCTACCAGATAAGTcggcag                                <  1:218274/251‑1 (MQ=255)                                                                                                                                                                                                                   gTTTGTTCAACAATGTGGATTTGCCAACGTGCGGACATCCGACGATGGCAATAAATCCGCAGTAACATTTAACGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACTGCGGCTTGAACCTGTGCCAACCACCGGGTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAGATAAGTcggcagcg                              <  2:13879/251‑1 (MQ=255)                                                                                                                                                                                                                       gTTCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTGCCAACCACCGGTGCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCTTGCGCTTCGCCACGTGCCTGGACTACCAGATAAGTcggcagcggcag                          <  1:21130/251‑1 (MQ=255)                                                                                                                                                                                                                         tCAACAATGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATACTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGTTCGTGCGCTTCGCCACGTACCTGGACTACCAGATAAGTcggcagcggc                            >  2:379398/1‑247 (MQ=255)                                                                                                                                                                                                                          caacaaTGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAGATAAGTCGGCAGCGGCAGAt                        <  2:225570/250‑1 (MQ=255)                                                                                                                                                                                                                              aaTGTGGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGTGCTTCGCCACGTACCTGGACTACCAGATAAGTCGGCAGCGGCAGATGGCg                    >  1:33448/1‑250 (MQ=255)                                                                                                                                                                                                                                 gtgGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCCCGTACCTGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGAcc                 <  2:308980/250‑1 (MQ=255)                                                                                                                                                                                                                                 gtgGATTTGCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGAccc                >  1:87598/1‑251 (MQ=255)                                                                                                                                                                                                                                         gCCAACGTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAat         <  1:376874/250‑1 (MQ=255)                                                                                                                                                                                                                                               gTTCGGACATCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAGATAAGTCGGCCGCGGCAGATGGCGACCCTGCAAATATTCTTg  >  2:316574/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   CTTCAACGACAAAAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTGCCAACCACCGGTTCACTCAGGCCGCTGACCTGGCAGTGGATAGTAAATTCCTGATCGTGCGCTTCGCCACGTACCTGGACTACCAGATAAGTCGGCAGCGGCAGATGGCGACCCTGCAAATATTCTTG  >  NC_000913/2703095‑2703583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑