Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,643,926 A→G S120P (TCT→CCT)  rlmN ← 23S rRNA m(2)A2503 methyltransferase/tRNA m(2)A37 methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,643,9260AG96.1% 146.3 / NA 52S120P (TCT→CCT) rlmN23S rRNA m(2)A2503 methyltransferase/tRNA m(2)A37 methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (16/33);  minor base T (0/2);  total (16/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

CCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGAGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAACCACTTCCGGTGCGCGGATTTCCGCCACCTCTTTCAGTTTGCCGCGCAACACTTTGTTGATGTCGGTCATCTCATCAAAGTTGTCGCAGCAATAGTGATACATCCACTTCATCACCTGATCGGCGCGGAAGGGT  >  NC_000913/2643679‑2644170
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    
ccAAAACCGAAATCATCAAGCAGGATTTCCAGCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCG‑GTTA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGAc                                                                                                                                                                                                                                                   <  2:296244/251‑1 (MQ=255)
  aaaaCCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGAGATCGGACGCTGACCG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGc                                                                                                                                                                                                                                                 >  2:351326/1‑251 (MQ=255)
             caccaAGCATGATTGCCATCCCCGGAACGACGTTGTTCAGGGTGAGCAGCTGCTCGCCCATGCCCAGCATCCCTACGTTAGTGATCGGACGCTGACCG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCTCACCTGCGAAGTGACGCAGAGCGTGGc                                                                                                                                                                                                                                       <  2:406811/247‑1 (MQ=255)
                  aGCATGATTTCCATCGCCGGAACGACGTTGGTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGgtc                                                                                                                                                                                                                                 <  1:250857/251‑1 (MQ=255)
                       gATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCtt                                                                                                                                                                                                                            >  1:174536/1‑251 (MQ=255)
                       gATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCt                                                                                                                                                                                                                             <  1:347384/250‑1 (MQ=255)
                            ccATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCgg                                                                                                                                                                                                                        <  1:413403/250‑1 (MQ=255)
                              aTCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGat                                                                                                                                                                                                                     <  1:320911/251‑1 (MQ=255)
                               gcGCCGGAACGACGTTGTTCATGTTGAGTAGCGGCTCGCCCAGTCCCAACATGACTACGTTAGGGATCGGACGCTAACCG‑GTGC‑GTTTTGCTCCGCCGACGATTCACGCCGCACGCCACACCTGGCCGATAATTTCCTACACCCGCAGTTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAAATTACACTGCAGCTCACACCCCACCAGGTAAGCGACGCAGAGCGTgggtcgtacgtgtttcgggata                                                                                                                                                                                                                    <  2:166587/250‑21 (MQ=255)
                               ccgctggatcgtcgtgtttCAGGTTGAGCAGCGGCTCGCCCATGCCCATAATCCCTACGTTAGGGATCGGACGCTGACCG‑GGGA‑CTTTTGCTGCGCCGACGATTTGCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAAGTTACACTCCAGCGCACACCCCACCTGCGAAGGTACGCAGAGCGTGGCACGTTCGTCTTCCGGGata                                                                                                                                                                                                                    <  2:277020/234‑1 (MQ=255)
                                 gCCGGAACTACTGTGTTCAGGTTGGGAAGCGGCTCGCCCATGCCTGTCATCGCTCCGTTAGTGATCGGACGCTGACCG‑‑TGTTCTTTTGCTGCCCCGACGATTGTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACGCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGGCGTCATCCGGGAtata                                                                                                                                                                                                                  <  2:24654/251‑1 (MQ=255)
                                 gCCGGAACGACGTGGTTCAGGTTGGTCAGCGGCTCGCCCATGCCCGTCATCCCTCCGTTAGTGATCGGACGCTGACCG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATGTACACTCCAGCGCACACCCCACCTTCGAAGGGACGCAGAGCGTGTCACTGTCTGCTTCCGGGAtata                                                                                                                                                                                                                  <  2:80576/251‑1 (MQ=255)
                                    ggAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGGCGCTGAACG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGAAGGTTGCGGTTAAAGCACTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATCTacac                                                                                                                                                                                                               >  1:189461/1‑251 (MQ=255)
                                                  cAGGTTGAGCCGCGGCTCGCCCATACCCATCATCACTACGTTAGTGATCGGACGCTGACCG‑GTGA‑CTTTTGCTGCGCCGACGATTTTAGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGACGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACCCCGTTTCGACCCGCt                                                                                                                                                                                                 >  2:42782/1‑251 (MQ=255)
                                                  cAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCG‑GCGA‑CTTTTGCTGCGCTGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACCCCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGGGGAACAGAATTTACACTCCAGCGCACACTCCCCCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACgcgc                                                                                                                                                                                                  <  1:282582/250‑1 (MQ=255)
                                                  cAGGTTCAGCATCGGCTCGCCCATGCCCATCATCCCTACGTTAGTGATCGCACGCTGACAG‑TTGA‑CTTTTGCAGCGCCGACGATTTTCGCCACACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGTGCGGTGGAACAGAATGTACACTCCAGCGCACTCCCCACCTGCGAAGGGACGCAGAGCGTTGCTCGGGCGTCTTCTTGGATATACCACGTTTCGACGCGCt                                                                                                                                                                                                 <  2:303147/251‑1 (MQ=255)
                                                       tGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGTACGCTGACCG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATc                                                                                                                                                                                             <  2:34120/250‑1 (MQ=255)
                                                            agcGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAAc                                                                                                                                                                                       <  1:130101/251‑1 (MQ=255)
                                                             gcGGCTCGCCCTTGCCCATCATCACTCCGTTATTGATCGGACTCTG‑CCGTGTGA‑CTTTTGCGGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCGTCCGGGATATACACCGTTTCGACGCGCTGATcttcaacg                                                                                                                                                                                      <  2:280154/251‑8 (MQ=255)
                                                               ggCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGGTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGc                                                                                                                                                                                    >  1:402104/1‑251 (MQ=255)
                                                                  tCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGAt                                                                                                                                                                                 >  1:92539/1‑251 (MQ=255)
                                                                       cacgcccatCATCACTATGTTAGTGATCGGACGCTAACCG‑GTGA‑CTTTTGCGGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCTGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGccc                                                                                                                                                                            <  2:280693/248‑1 (MQ=255)
                                                                                    aCTACGTTAGTGATCGGACGATGACCG‑GTGA‑CTTTTGCTGCGCCGCCGATTTTCGCCGCACGCAACACCTGGCCGATAATTCCCGCAACCAGCAGGTTGCGGCTAAAGACCTGCTGTGCGGGGGCACAGAATTTAAACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGTGCGTGGCACGGTCGGCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGCTCGCCCATTTCATGATGcc                                                                                                                                                               >  2:410403/1‑251 (MQ=255)
                                                                                       aCGTTAGTGATCGGACGCTGACCG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTc                                                                                                                                                            <  1:173612/251‑1 (MQ=255)
                                                                                        cGTTAGTGATCAGCCGCAGTCCG‑GTGA‑CTTTTGCTGCGCCGACAATTTTCGCCGCCCGCCACACCTGGCTGATAATTTCCGACAACCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGATCAGAATTTTCACTCCAGCGTGCACCCCGCTTGCGAAGTGACGCGGAGCGTGGCACGGTCGTCTTCTGTGGTATACACCGTTTCGACGCGGTTATCGCTAACGGCGATCGGCGATTTAATgaagcagtca                                                                                                                                                           <  2:210551/251‑10 (MQ=255)
                                                                                              tGATCGGACGCTGACCG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAAc                                                                                                                                                                                       >  2:321861/1‑217 (MQ=255)
                                                                                              tGATCGGACGCTGACCG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAAc                                                                                                                                                                                       <  1:321861/217‑1 (MQ=255)
                                                                                                     ccGCTGACCG‑GTGA‑CTTTTGCGGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATGTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGtt                                                                                                                                              <  2:70660/250‑1 (MQ=255)
                                                                                                             cG‑GTGA‑CTTTTTCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCATAGCTTGGCATGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAAcc                                                                                                                                      <  1:276921/251‑1 (MQ=255)
                                                                                                             cG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACTCCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCTGTGGAACAGAATTTTCCCTCCAGCGCACACCCCACCTGCGAAGGGACGCAGTGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAAcc                                                                                                                                      <  1:63152/251‑1 (MQ=255)
                                                                                                              g‑gTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAAcc                                                                                                                                      >  1:100024/1‑250 (MQ=255)
                                                                                                                            tgcaccGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCCCGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAAAGCTGGTCTTCAACCACTTCCGGTgcgc                                                                                                                         >  2:397388/5‑251 (MQ=255)
                                                                                                                                     gaTTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACGCTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTTCCGTCAGATGAACGCTGTTCTTCAACCACTTCCGGTGCGCGGATTTCCg                                                                                                                <  1:27624/251‑1 (MQ=255)
                                                                                                                                                cACGCCACACCTGGCCGCTAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGAGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGAGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCTTCAGCTGAACGCTGTTCTTCACCCACTTCCGGTGCGCGGATTTCCGCCACCTCtttt                                                                                                     >  2:146642/1‑250 (MQ=255)
                                                                                                                                                 aCGCCACACCTGGCCGATAATTGCCGACACCCGCAGGTTGCGGTTAAAGCCATGCTGGGCGGTGGAACAGAATTTACACTCCGGCGCACACCCCACCTGCGAAGGTACGCAGAGCGGGGAACGGCCGTCGTCCGGTATATACACCGTTTGGCCGCGCTGATCGCCAACGGCGATCGCCCATTTAATTGTTCCCTCAGAAGCACGCTGTTATTCCACCACTACCGGGTCGCGGATTACGCCCACCTCTTTc                                                                                                     >  2:223924/1‑250 (MQ=255)
                                                                                                                                                  cGCCACACCTGGTCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCTCCGGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAACCACTTCCGGTGCGCGGATTTCCGCCACCTCTTTCAg                                                                                                   <  1:200067/251‑1 (MQ=255)
                                                                                                                                                       cacCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAACCACTTCCGGTGCGCGGATTTCCGCCACCTCTTTCAGTTTGc                                                                                              >  2:205944/1‑251 (MQ=255)
                                                                                                                                                                           acacCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAACCACTTCCGGTGCGCGGATTTCCGCCACCTCTTTCAGTTTGCCGCGCAACACTTTGTTGATg                                                                          >  1:323784/1‑251 (MQ=255)
                                                                                                                                                                             acCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAACCACTTCCGGTGCGCGGATTTCCGCCACCTCTTTCAGTTTGCCGCGCAACACTTTGTTGATGTc                                                                        <  1:298807/251‑1 (MQ=255)
                                                                                                                                                                              cccGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTGTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAACCACTTCCGGTGCGCGGATTTCCGCCACCTCTTTCAGTTTGCCGCGCAACACTTTGTTGATGTCg                                                                       <  1:205944/251‑1 (MQ=255)
                                                                                                                                                                                 gCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAACCACTTCCGGTGCGCGGATTTCCGCCACCTCTTTCAGTTTGCCGCGCAACACTTTGTTGATGTCg                                                                       >  2:87676/1‑248 (MQ=255)
                                                                                                                                                                                 gCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAACCACTTCCGGTGCGCGGATTTCCGCCACCTCTTTCAGTTTGCCGCGCAACACTTTGTTGATGTCg                                                                       <  1:87676/248‑1 (MQ=255)
                                                                                                                                                                                                aGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAACCACTTCCGGTGCGCGGATTTCCGCCACCTCTTTCAGTTTGCCGCGCAACACTTTGTTGATGTCGGTCATCTCATCAAAGTTg                                                     <  1:96562/251‑1 (MQ=255)
                                                                                                                                                                                                      gctgGGCGGTGGAACAGAATGTACACTCCAGCGCACAACCCACCGGCGAAGGGACGCAGAGCGGGGCACTGTCGTCTTCCGGGTTATACTCCGTTTAGACGCGCTGAGCGCCAACGGCGATCGTCCATTTATTGGTGCCGTCAGATGAACTCTGTTCTTCATCCACTTCCGGTGCGCGGATTTCCGCCACCTCTTTCAGTTTGCCGCGCAACACTTTGTTGATGTCGGGCATCTCATCAAAGTTGGCgcag                                               <  2:225850/251‑1 (MQ=255)
                                                                                                                                                                                                           gCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGGCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAACCACTTCCTGTGCGCGGATTTCCGCCACCTCTTTCAGTTTGCCGCGCAACACTTTGTTGATGTCGGACATCTCATCAAAGTTGTCGCAGCAATa                                          <  2:412569/251‑1 (MQ=255)
                                                                                                                                                                                                                    cAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAACCACTTCCGGTGCGCGGATTTCCGCCACCTCTTTCAGTTTGCCGCGCAACACTTTGTTGATGTCGGTCATCTCATCAAAGTTGTCGCAGCAATAGTGATACAt                                 <  1:303483/251‑1 (MQ=255)
                                                                                                                                                                                                                       aaTTTACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAACCACTTCCGGTGCGCGGATTTCCGCCACCTCTTTCAGTTTGCCGCGCAACACTTTGTTGATGTCGGTCATCTCATCAAAGTTGTCGCAGCAATAGTGATACATCCa                              <  1:239928/251‑1 (MQ=255)
                                                                                                                                                                                                                           tACACTCCAGCGCACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAACCACTTCCGGTGCGCGGATTTCCTCCACCTCTTTCAGTTTGCCGCGCACCACTTTGTTGATGTCGGTCATCTCATCAAAGTTGTCGCAGCAATAGTGATACATCCACTtc                          >  2:359597/1‑251 (MQ=255)
                                                                                                                                                                                                                                      gcACACCCCACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAACCACTTCCGGTGCGCGGATTTCCGCCACCTCTTTCAGTTTGCCGCGCAACACTTTGTTGATGTCGGTCATCTCATCAAAGTTGTCGCAGCAATAGTGATACATCCACTTCATCACCTGATc               <  2:92539/251‑1 (MQ=255)
                                                                                                                                                                                                                                            cccACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAACCACTTCCGGTGCGCGGATTTCCGCCACCTCTTTCAGTTTGCCGCGCAACACTTTGTTGATGTCGGTCATCTCATCAAAGTTGTCGCAGCAATAGTGATACATCCACTTCATCACCTGATCGGcgcg         <  1:113508/251‑1 (MQ=255)
                                                                                                                                                                                                                                             ccACCTGCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGGGCCGTCAGATGAACGCTGTTCTTCAACCACTTCCGGTGCGCGGATTTCCGCCACCTCTTTCAGTTTGCCGCGCAACACTTTGTTGATGTCGGTCATCTCATCAAAGTTGTCGCAGCAATAGTGATACATCCACTTCATCACCGGATCGGCGCgg        <  2:402104/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   gCGAAGGGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAACCACTTCCGGTGCGCGGATTTCCGCCACCTCTTTCAGTTTGCCGCGCAACACTTTGTTGATGTCGGTCATCTCATCAAAGTTGTCGCAGCAATAGTGATACATCCACTTCATCACCTGATCGGCGCGGAAGGGt  <  1:328195/251‑1 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    
CCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCG‑GTGA‑CTTTTGCTGCGCCGACGATTTTCGCCGCACGCCACACCTGGCCGATAATTTCCGACACCCGCAGGTTGCGGTTAAAGCCCTGCTGGGCGGTGGAACAGAATTTACACTCCAGCGCACACCCCACCTGCGAAGAGACGCAGAGCGTGGCACGGTCGTCTTCCGGGATATACACCGTTTCGACGCGCTGATCGCCAACGGCGATCGCCCATTTAATGGTGCCGTCAGATGAACGCTGTTCTTCAACCACTTCCGGTGCGCGGATTTCCGCCACCTCTTTCAGTTTGCCGCGCAACACTTTGTTGATGTCGGTCATCTCATCAAAGTTGTCGCAGCAATAGTGATACATCCACTTCATCACCTGATCGGCGCGGAAGGGT  >  NC_000913/2643679‑2644170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑