Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,518,034	G→A	intergenic (‑202/+7)	pdeA ← / ← alaX	putative c‑di‑GMP phosphodiesterase PdeA/tRNA‑Ala

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,518,034	0	G	A	100.0%	41.0 / NA	14	intergenic (‑202/+7)	pdeA/alaX	putative c‑di‑GMP phosphodiesterase PdeA/tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/7);  total (7/7)

TTTTTTATCAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTCCGCC  >  NC_000913/2517804‑2518280                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       ttttttATCAGGGTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTAGAAATTTGGTGGAGCTCAGc                                                                                                                                                                                                                                    >  1:329917/1‑251 (MQ=255)                                                gATCGGGGTTTTCAGGCACACAAATAAGAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTAGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCa                                                                                                                                                                                      <  1:171973/250‑1 (MQ=255)                                                gATCGGGGTTTTCAGGCACACAAATAACAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTGCATCCAATGATGCTTTCACCAGGCGGCGCTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTAGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAg                                                                                                                                                                                     <  2:29860/251‑1 (MQ=255)                                                 aTCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTAGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGc                                                                                                                                                                                    <  1:338296/251‑1 (MQ=255)                                                         tttCAGGCACACAAATCATAAGTTTATTCGCGTCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAACGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTAGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCta                                                                                                                                                                             >  2:238087/1‑250 (MQ=255)                                                                   acaAAGAATAAGTTTATGCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGAGGCTTTCACCAGGCGGCACTAACCCGGACTTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTAGAAATGGGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGa                                                                                                                                                                  <  1:157523/251‑1 (MQ=38)                                                                    caAATAATCAGTTTATTCGCCTCGCGAAGCAAACAGGGGGCCAGCATGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGCCGTAGACTGTATTACAACAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTAGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGAt                                                                                                                                                                 >  2:22710/1‑251 (MQ=255)                                                                      aagaataaGTTTAGTCGCATCGCGAAGCAATCAGGGGGCCAGCCTGTTGTCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACTTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTAGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGTCCTCGTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGc                                                                                                                                                               <  1:22710/248‑1 (MQ=38)                                                                                                  aaaCAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTAGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTgac                                                                                                                                    >  2:99727/1‑248 (MQ=255)                                                                                                                                                           aCTAAACCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCGTTGCAGCGAGGGTTAGAAATCTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTGTACGGACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAgcgc                                                                          <  1:197732/251‑1 (MQ=255)                                                                                                                                                               accaggACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCACCGCTTTGCAGCGAGGGTTAGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCATGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACATCTTGCATGCCATGCAAGCGCtctc                                                                      >  2:132566/5‑251 (MQ=255)                                                                                                                                                                                     aaaaGCGGCAAAAAGCAGAGACAAAAAACACCCGCTTTGCAGCGAGGGTTAGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTc                                                >  1:132882/1‑251 (MQ=255)                                                                                                                                                                                       aaGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTAGAAATTTGGTGGCGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGAAAGCGCTCTCCCAGCTGAGCTCTAGCCACGTccc                                              >  2:374843/1‑249 (MQ=255)                                                                                                                                                                                                                                   ggTTAGAAATGTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTCCGcc  <  1:209534/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       TTTTTTATCAGGTTATGCTCCACAAACATGAGTACACTACTCTCACTGATCGGGGTTTTCAGGCACACAAATAATAAGTTTATTCGCATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCGTCACGTAAAGCTTGTCGAGTTGACGGGCGGCATCATATGAATTCCGCC  >  NC_000913/2517804‑2518280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑