Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,495,182	G→A	I37I (ATC→ATT)	yfdY ←	DUF2545 domain‑containing protein YfdY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,495,182	0	G	A	100.0%	56.0 / NA	21	I37I (ATC→ATT)	yfdY	DUF2545 domain‑containing protein YfdY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/8);  total (13/8)

GACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTG‑AACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGCAATTTTTGCTAAGCATCAATGAAAACAGCATGTTAAATGCAAGACTGTTG  >  NC_000913/2494937‑2495417                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            gACAAAGGCAAATTATCTTTTTCATCCTGTGCGAAACCGTTTTTCACATGGCAGGTG‑AACAACCATGTCTGATGCTGAGGTGACCACCGGCCCAAAGGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACGAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGAATCAc                                                                                                                                                                                                                                        <  2:167248/251‑1 (MQ=255)  aCAAAGGCGAATTATCTTATTCATCCTGTGCGTTAGCGTCTTTCACATGGCAGGTG‑AACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATACCCGTCACTAGTTCATTACCGCACGTTAACCACATGGTGACGTAATAAATGAGAGCAGCGAGAATCaac                                                                                                                                                                                                                                       >  2:232095/1‑249 (MQ=255)  aCAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTG‑AACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGAATCAcc                                                                                                                                                                                                                                       >  1:90167/1‑251 (MQ=255)           atttATCTTATTAATCCTGTGGGATAGCGTTTTTCACATGGC‑GGTGTAACAATCATGTCTGATGCTGTTGTAAACACCTGCCCAAAGGAGCAATCCTGACCCGCTGCCGCCTGCCATACCCCACCATAAAGGGAATCATCAAGCCTCAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATGACCGCTAGTTAACCACATGGTGAAGTAAGAAATGAGTGCAGCGAGAAGCACCATGCCgaag                                                                                                                                                                                                                              <  2:209480/249‑1 (MQ=255)                                 gATAGCGTCTTTCACATGGCAGGTG‑AACAATCCTGTCTGATGCTGCTGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTCCCGCCTGCCATACCCCACCATCAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGAATCACCATGCCGAAGAAAGAAGAGACCGCCGATAcc                                                                                                                                                                                                         >  2:412144/1‑250 (MQ=255)                                                aTGGCAGGTG‑AACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACCTAAATATCCCCGTCACTAGTTCCTTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGAATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGAc                                                                                                                                                                                         >  1:275214/1‑251 (MQ=255)                                                      ggTG‑AACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGAATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGt                                                                                                                                                                                   >  1:169750/1‑251 (MQ=255)                                                                                                                   cTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGAATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAAc                                                                                                                       >  1:248175/1‑251 (MQ=255)                                                                                                                                     ccccaaaaagcaaaccaccaaaccACAATCCGGGGCAAGAAACAAAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAAGGAGTGCAGCGAGAATCGCCATGCCGAAGAAAGAAGAGACCGCCGATACCACTTTCAGTACCTGGCCGATGTAGCCGCTTACGCAGACAATACTCAGTGCGAGAAACATCCATAAATTAATCATAGCTCAACCACCTGCCTCGCTaaaag                                                                                                     <  2:30358/229‑2 (MQ=255)                                                                                                                                      ccaaaaagccaaaacacaaacccACAAGCCGGGCCAAGAAACATAAATACCCCCTCCACTATTCCATTCCCCCAATTTACCCTCATGGGAAAGTAAAAAATGAGGGCAGCGAGAATCTCCATGCCGAAGAAAAAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACTCAGACAATACACAGTGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTaaaaaa                                                                                                    <  1:25257/230‑1 (MQ=255)                                                                                                                                                             acaAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGAATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGAt                                                                                                                                                                                     >  1:168337/1‑147 (MQ=255)                                                                                                                                                                              aaaaaaagaccccctccACTATTTCATCCCCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCGGCGAAAACCCCCATGCCAAAGAAAAAGGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTaa                                                            <  1:223995/235‑1 (MQ=255)                                                                                                                                                                              aCATAAATATCCCCGTCACTAGTTCATTACAGCCCGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGAATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTa                                                             >  1:310122/1‑250 (MQ=255)                                                                                                                                                                                                  tgTTCATTCCCGCAAGTAAACCAAAGGGAGAAGTAATAAAAGAGTACAGCGAGAACCACCATGCCGAAAAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGCAATGTTTGCTa                                         <  2:169750/249‑1 (MQ=255)                                                                                                                                                                                                  aGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGAATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGCAATTTTTGCTa                                         >  1:205991/1‑250 (MQ=255)                                                                                                                                                                                                     ttatgcccGCAAGTTAACCACATGGTGAAGTAAAAAATGAGGGCAGCGATAACCACCATGCCGAAGAAAGAAGAGGCCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGTCGCTCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGCAATTTTTGCTAAGc                                      <  1:179408/244‑1 (MQ=255)                                                                                                                                                                                                       aTTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGAATCACCATGCCGAAGAAAGAAGAGACCGCCGATACAACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTAACTTTAATAGTAAGACAATCCTTAACCTCTGGCAATTTTTGCTAAGCATc                                   >  1:217871/1‑251 (MQ=255)                                                                                                                                                                                                             gCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGAATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGCAATTTTTGCTAAGCATCAATGa                              >  2:224211/1‑250 (MQ=255)                                                                                                                                                                                                                      ccACATGGTGAAGTAATAAATGAGTGCAGCGAGAATCACCATGCCGAAAAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGCAATTTTTGCTAAGCATCAATGAAAACAGCATg                    <  1:409213/251‑1 (MQ=255)                                                                                                                                                                                                                               gAAGTAATAAATGAGTGCAGCGAGAATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGCAATTTTTGCTAAGCATCAATGAAAACAGCATGTTAAATGc            >  1:410444/1‑250 (MQ=255)                                                                                                                                                                                                                                        aaTGAGTGCAGCGAGAATCACAATGACGAAGAAAGAAGAGACCGCCGATACCACATTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGCGAGAAACATCCATAACTTAATCATAGCTCAACCTCATGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGCAATTTTTGCTAAGCATCAATGAAAACAGCATGTTAAATGCAAGACtgttg  >  2:401399/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            GACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTG‑AACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGCGAGAAACATCCATAAATTAATCATAGCTCAACCTCCTGCCGCGCTAAAAAACCTGTAACTTCAGTCACTTTAATAGTAAGACAATCCTTAACCTCTGGCAATTTTTGCTAAGCATCAATGAAAACAGCATGTTAAATGCAAGACTGTTG  >  NC_000913/2494937‑2495417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑