Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,426,454	G→A	A112V (GCC→GTC)	hisJ ←	histidine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,426,454	0	G	A	96.9%	91.8 / ‑5.3	32	A112V (GCC→GTC)	hisJ	histidine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (16/15);  minor base C (1/0);  total (17/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATAATTCCTTTGCCAGATCGATATCGAAGCCAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACC  >  NC_000913/2426209‑2426698                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     gttTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCATGACAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGACCGTCTCCTGGGTGGTTCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGTCTCGACTGTCGTCTGAATGTCAGAATTTTTCGCCAACACCAAACGTGAACCGACAGCg                                                                                                                                                                                                                                                 >  2:101681/1‑251 (MQ=255)     gAGGAAACCTTCGCTGGCAGCGGCCTCATCCTGGAACGCGGCATCAATACGTACGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGCGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGAAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGTATCGACAGCGtcc                                                                                                                                                                                                                                              >  1:211190/1‑248 (MQ=255)                          gACCTCATCCCGGAACGCGGCATCAATACGTCCGTCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCCTTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGCCTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAACCGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTAttt                                                                                                                                                                                                                        >  2:198173/1‑251 (MQ=255)                                         tgcgGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGttttt                                                                                                                                                                                                         >  1:246426/2‑251 (MQ=255)                                         cgcgGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGttttt                                                                                                                                                                                                         >  1:246435/1‑251 (MQ=255)                                                              aGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGAtaac                                                                                                                                                                                     >  2:313372/1‑247 (MQ=255)                                                                 caggtcagAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAAGGGAAAGCGATGACatg                                                                                                                                                                                  <  2:207519/250‑1 (MQ=255)                                                                       agAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGCTGACATGATGGCGt                                                                                                                                                                           >  2:179267/1‑251 (MQ=255)                                                                        gAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTc                                                                                                                                                                          <  2:80365/251‑1 (MQ=255)                                                                            aaaTGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTTCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGTAAAGCGATGACATGATGGCGTCAAt                                                                                                                                                                       <  1:384137/250‑1 (MQ=255)                                                                                  tgtCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTACCCAATGTTCATTACCGAACGTCTCCTGGGTGGAGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTGCCGTAAGGGAAAGCGATGACATGATGGCGTCAATcttctt                                                                                                                                                                 <  2:211275/250‑1 (MQ=255)                                                                                            cTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAgg                                                                                                                                                      >  2:304495/1‑251 (MQ=255)                                                                                                                    ttttGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGTTTAACGCATCCAGCggct                                                                                                                              >  1:214313/1‑249 (MQ=255)                                                                                                                     tttGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATc                                                                                                                                      <  1:123033/242‑1 (MQ=255)                                                                                                                     tttGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATc                                                                                                                                      >  2:123033/1‑242 (MQ=255)                                                                                                                                    tCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTAc                                                                                                               <  1:227905/250‑1 (MQ=255)                                                                                                                                         aCCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCg                                                                                                         <  1:267685/251‑1 (MQ=255)                                                                                                                                                            gtgCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATa                                                                                       >  2:323859/1‑250 (MQ=255)                                                                                                                                                                 cTGCAACACGCCTACCCGTTTACCTTTCAGCGAATCGCCTGTCGGCTGAATGTAAGAATTTTTCGCCACCCCCAAACGAGAATCGACAGCGTACAGATTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTACTGGAAAGCGATGACATGCTGGAGTCAATCTTCTTCGCTTTTCAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATACTTCCt                                                                                 >  2:394969/1‑251 (MQ=255)                                                                                                                                                                   gCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGCCTGTCGGCGGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGTCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATAATTCCt                                                                                 <  2:316845/249‑1 (MQ=255)                                                                                                                                                                   gCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATAATTCCtt                                                                                <  1:66447/250‑1 (MQ=255)                                                                                                                                                                        aCGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATAATTCCTTTGcc                                                                            >  1:407551/1‑249 (MQ=255)                                                                                                                                                                                  gtgtgctctcctacgagacgtctGTCGGATGTATGTCAGTATTTTCCGCAACCATCAAACCAGAATCGACCTCGTACAGGTTGGCGGTGAAGGCTATTTCTTGCTGTCGTTTTGCCGTAATGGAAAGCGATGACATGATTGCGTCAAGCTTCTTCGCTTTTAAGTACGGGATTAACGAATCCAGCGGATTTTCGACAAACGTACATTGCTTATTGATGCGTTGGCATAATTCCTCTTCCAGAGTGATATCg                                                                <  2:3378/230‑1 (MQ=255)                                                                                                                                                                                  gTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATAATTCCTTTGCCAGATCGATATCg                                                                >  1:412099/1‑251 (MQ=255)                                                                                                                                                                                             aGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATAATTCCTTTGCCAGATCGATATCGAAGCCAACCAg                                                     <  1:123891/251‑1 (MQ=255)                                                                                                                                                                                             aGCGACTCGACTGTCGGCTGAATGTCAGAATTTGTCGCCACCACCAAACGAGAATCGACAGCTTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATAATTCCTTTGCCAGATCGATATCGAAGCCAACCAg                                                     <  2:405503/251‑1 (MQ=255)                                                                                                                                                                                                     gactGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATAATTCCTTTGCCAGATCGATATCGACGCCAACCAGTTCGCCtt                                             >  1:147082/1‑251 (MQ=255)                                                                                                                                                                                                       ctGTCGGCTGACTGTCAGACTTTTTCGCCACCACCAAACGAGAATCGCCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATC‑AGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATAATTCCTTTGCCAGATCGATATCGAAGCCAACCAGTTCGCCTtgtg                                          >  1:343296/1‑251 (MQ=255)                                                                                                                                                                                                         gTCGGCTGAATGTCAGAATTTTGCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTGCTTGCTGACGTTTGTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTGAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTGGCATAATTCCTTTGCCAGATCGATAGCGAAGCCAACCAGTTCGCCTtgt                                           <  2:211190/249‑1 (MQ=255)                                                                                                                                                                                                                          aTTTTTCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATAATTCCTTTGCCAGATCGATATCGAAGCCAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATg                        <  2:115269/251‑1 (MQ=255)                                                                                                                                                                                                                              ttCGCCACCACCAAACGAGAATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACAGTGCGTATTGATGCGTTTGCATAATTCCTTTGCCAGATCGATATCGAAGCCAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGgcg                     <  1:198173/250‑1 (MQ=255)                                                                                                                                                                                                                                                gaATCGACAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATAATTCCTTTGCCAGATCGATATCGAAGCCAACCAGTTCGCCTTGTGAATTTTTTGATTCAACTGGCGCATAGGTCGGGTCGGTAcc  >  2:168217/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     GTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATAATTCCTTTGCCAGATCGATATCGAAGCCAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACC  >  NC_000913/2426209‑2426698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑