Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,114,750	G→A	Q383* (CAA→TAA)	wcaM ←	putative colanic acid biosynthesis protein WcaM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,114,750	0	G	A	94.1%	41.1 / ‑5.4	17	Q383* (CAA→TAA)	wcaM	putative colanic acid biosynthesis protein WcaM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (6/10);  minor base C (0/1);  total (6/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGGCGCTATTAATCATTTCAATATTATCAATCACGAAAT  >  NC_000913/2114507‑2114992                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   cctgcAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACATTATGGTGATTAATCCTGTCGATAGCGACGGAGCTTTGTCCTGTTTCATTCACCGCATGAACATTTGCAAGATACAGTAGTGTTTCTTTTTTCGCCATATAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGTTAAATGCGGGTCCGACTGAGGATTCCTGCATCACAGTGATATTCCGCATAAAAAAATGTTCCGGtt                                                                                                                                                                                                                                               <  1:190428/245‑1 (MQ=255)     cAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAATGTTACGGTttgtt                                                                                                                                                                                                                                           <  2:338016/249‑1 (MQ=255)                                                 ggTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAATGTTACGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGcc                                                                                                                                                                                             <  1:233048/251‑1 (MQ=255)                                                                                 tGTCCTTTTTCCTTCACCGCTTGAACCTTTGCACGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGCACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCAAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAATGTTAAGGTTTGTTGTGTAACTACAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTg                                                                                                                                                             >  2:261004/1‑251 (MQ=255)                                                                                  gTCCTTTTTCCTTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAATGTTACGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGa                                                                                                                                                            <  2:234442/251‑1 (MQ=255)                                                                                  gTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAATGTTACGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTg                                                                                                                                                             <  2:338849/250‑1 (MQ=255)                                                                                           cattcaCCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAATGTTACGGTTTGTTGTGTAACTCCAGCTACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCTGAGATTTGAATGCcgcg                                                                                                                                                    <  1:143481/250‑1 (MQ=255)                                                                                                                          cagTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAATGTTACGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGtt                                                                                                                    >  1:263694/1‑251 (MQ=255)                                                                                                                                            cATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAATGTTACGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGTAGATTTGAATGCCGCGCAATTTATAAGCAAGATGATTATTATCCAGTTGAATATCATACACCCGGa                                                                                                  <  2:393504/251‑1 (MQ=255)                                                                                                                                                           aaCGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAATGTTACGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAATATCATTCACCCGGAAATTTTGCGGTATCg                                                                                   >  2:365478/1‑251 (MQ=255)                                                                                                                                                                    gcgcATGTCAAAGTTCATGCTCAATGCGGGTTCAACTGAGTATTCCTGCATCAAATTGATATTTCTCATAAAAAAATGTTACGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTACCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTAGTATCCAGTTTAATATCATTCACCCGGAAATTTTGCGTTATCGAGAGatat                                                                           <  1:81942/250‑1 (MQ=255)                                                                                                                                                                     cgcATGTCAAAGTTCATGCTTAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAATGTTACGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAATATCATTCACCCGGAAATTTTGCGGTATCTAGatatatat                                                                         <  2:282851/251‑3 (MQ=255)                                                                                                                                                                             aaaGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAATGTTACGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTat                                                                 >  2:293494/1‑250 (MQ=255)                                                                                                                                                                                                      aCTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAATGTTACGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATccc                                        <  1:139463/251‑1 (MQ=255)                                                                                                                                                                                                                   tcATCACATTGATATTTCGCATAAAAAAATGTTACGGTTTGTTTTGTAACGACAGCGACGCACGCTTCAACTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGTAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCATTGAATTACCCCATCGCCGATTAACATCCCGGCGCTATTAAt                            <  2:229182/249‑1 (MQ=255)                                                                                                                                                                                                                               tatTTCGCATAAACAAATGCTACGGTTTGTTGCGTAACTCCAGCGACGCACGCCTCATCTCAATGTTAGTTAGCGCCACAAAGGAAATCGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTACTTACCCCATACCCGATTAACATCCCGGCGCAATTGATAATTTCAAtatt                >  2:302657/1‑250 (MQ=255)                                                                                                                                                                                                                                            aaaaTGTTACGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCACGGCGCTATTAATCATTTCACTATTATCAATCACGaact  >  2:298355/1‑249 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   CCCTCAGTTCCGGCAATCTAAAGTTAATCTTCTCCACATTAACAATATGGTGATTAATCCTGTCGATATCGACGGAGCTTTGTCCTTTTTCATTCACCGCATGAACATTTGCAAGAGACAGCAGTGTTTCTTTTTTCGCCATAAAAACACCACGAACGTCTTTGCGCATGTCAAAGTTCATGCTCAATGCGGGTCCAACTGAGGATTCCTGCATCACATTGATATTTCGCATAAAAAAATGTTGCGGTTTGTTGTGTAACTCCAGCGACGCACGCTTCATCTCAATGTTAGTTAGCGCCACAAAGGAAACAGCATTCCCGGCGGAGATTTGAATGCCGCGCAATTTATAAGCAAGATGAGTATTATCCAGTTGAATATCATTCACCCGGAAATTTTGCGGTATCGAGAGATATTTGCCTTTAATTACCCCATAGCCGATTAACATCCCGGCGCTATTAATCATTTCAATATTATCAATCACGAAAT  >  NC_000913/2114507‑2114992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑