Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,966,684	C→T	L118L (CTG→CTA)	cheZ ←	chemotaxis protein CheZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,966,684	0	C	T	100.0%	105.7 / NA	34	L118L (CTG→CTA)	cheZ	chemotaxis protein CheZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/16);  total (18/16)

TACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTCCTGTTCCGGGATGTTTTCCAACAGCACCATCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGACGCCTCAACACTGTTCAGCGCCCGCTCCGCAGCCTGGGCGGTCATCTGCACAACATAGTACAAACGATCGCGCGCATCGGGGATGGCTTCCGCCGCTTCGGCAATG  >  NC_000913/1966441‑1966918                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           tACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTCCTGTTCCGGGATGTTTTCCAACAGCACCATCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGCACATCTGCTAGAAAtt                                                                                                                                                                                                                                     >  1:52740/1‑251 (MQ=255)                        cGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTCCTGTTCCGGGATGTTTTCCAACAGCACCATCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCa                                                                                                                                                                                                              <  1:343003/251‑1 (MQ=255)                         gACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTCCTGTTCCGGGATGTTTTCCAACAGCACCATCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCAc                                                                                                                                                                                                             <  1:367998/251‑1 (MQ=255)                             tGAGGCCCATTAAGAAAAATCTGGTTTTCCAGTTTTGGCCGCGCCTCCTGTTCCGGGATGTTTTCCAACAGAACCATCAGCAACTGGCGTTCGATATCATGAATGACCTCAATCATCCGCTTAATGACCTACCCGGTGAGATCCTGAAAATCCTGAGCCATCATGATTTCCAGCAGTTGAGCGTTAGTAAAGCTGGTATGCGCGGGTACCTATGATAGTAATTGTCGTGTATCTGTTACCAGTTCACGGGc                                                                                                                                                                                                         >  2:272780/1‑251 (MQ=255)                                ggTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTCCTGTTCCGGGATGTTTTCCAACAGCACCATCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTc                                                                                                                                                                                                      <  1:98875/251‑1 (MQ=255)                                           cAAACTCTGATTTTCACGTTTTGGACGAGCCTCCTGTTCCGGGATGTTTTACAACAGCACCATCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATACGATTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAATCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATATGTTACCAGGTAACGGGCGTCGGCAAggaca                                                                                                                                                                                            >  2:269081/1‑247 (MQ=255)                                                tctGGTTTTCACGTTTTGGACGCGACTCCTGTTCCGGGATGTTTTCCAACAGCACCATCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAatcgg                                                                                                                                                                                       >  1:338176/1‑250 (MQ=255)                                                   ggTTTTCACGTTTTGGACGCGACTCCTGTTCCGGGATGTTTTCCAACAGCACCATCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGCACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAgtcgga                                                                                                                                                                                      <  2:172317/248‑1 (MQ=255)                                                    gTTTTCACGTTTTGGACGCGACTCCTGTTCCGGGATGTTTTCCAACAGCACCATCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAatcggatcgg                                                                                                                                                                                  >  1:364233/1‑251 (MQ=255)                                                       ttCACGTTTTGGACGCGACTCCTGTTCCGGGATGTTTTCCAACAGCACCATCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGAGAGATCCTGAAAATAATGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCa                                                                                                                                                                                >  2:374476/1‑250 (MQ=255)                                                        tCACGTTTTGGACGCGACTCCTGTTCCGGGATGTTTTCCAACAGCACCATCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATTATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCaa                                                                                                                                                                               <  1:80650/250‑1 (MQ=255)                                                                tGGACGCGACTCCTGTTCCGGGATGTTTTCCAACAGCACCATCAGCAACTGGCGTTCGCTCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGCTTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCAt                                                                                                                                                                      >  2:365870/1‑251 (MQ=255)                                                                tGGACGCGACTCCTGTGCCGGGATGTTTGCCAACAGCACCATCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCGTAATGACCGGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTAGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTGACCAGTTCACGGGCGGCGGCAAGGTCAATCGGATCGGCAAACCAGTCa                                                                                                                                                                       <  1:211965/250‑1 (MQ=255)                                                                              gTTCCGGGATGTTTTCCAACAGCACCATCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTgtg                                                                                                                                                          <  2:364233/249‑3 (MQ=255)                                                                                          ttgtcaactgctccATCAGCAACTGGAGTTCGTTCTCCTGAATGACATCCTGCTTCCTCTTAATGATCTTCCCGGGGAGATCCTGAAAATCCTGCTCCATCAAGATTTCCAGCAGTGGCGCGTTAGTAAAGGTGGTATTGGCGGGTGCGTCTGCTAGAAATTGTCGGTTATGTGTTACCAGTTCGCTTGCGTCGGCACGGGCAAGCTTATCTGTAACCCAGTCATCCCAACGTTGGGTTAACGCTTTTGc                                                                                                                                             <  1:370467/238‑1 (MQ=255)                                                                                                        aTCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTg                                                                                                                              <  1:121195/251‑1 (MQ=255)                                                                                                            gCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCTTGAAAATCCTGCGCCATCAGGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCTCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACTTTGGGTTAACTCTTTTGCTGATGTCTTCATTTGATcc                                                                                                                          <  2:395632/251‑1 (MQ=255)                                                                                                                     gTTCGATATCCTGAATGCCATCCATCATCAGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGAGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCCCGGGCGTCGGCAAGGTCAATCGGTTCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTATGCTGATTTCTCCATTTGATCCTGATGCGGt                                                                                                                 >  2:202302/1‑251 (MQ=255)                                                                                                                              ccTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGACGcc                                                                                                        >  1:117228/1‑251 (MQ=255)                                                                                                                                                   gCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGACGCCTCAacac                                                                                                 >  2:128864/1‑237 (MQ=255)                                                                                                                                                   gCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGACGCCTCAacac                                                                                                 <  1:128864/237‑1 (MQ=255)                                                                                                                                                               gCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGACGCCTCAACACTGTTCAGCGCCCGCTCCGCAGCCTg                                                                        <  1:40246/250‑1 (MQ=255)                                                                                                                                                                    gTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGAGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCAAGGGCGCCGGCAAGGTAAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGACGCCTCAACACTGTTCAGCGCCAGCTCCGCAGCCTGGGCGGt                                                                  >  1:415500/1‑251 (MQ=255)                                                                                                                                                                            cTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACAAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGACGCCTCAACACTGTTCAGCGCCCGCTCCGCAGCCTGGGCGGTCATCTGc                                                           >  2:288664/1‑250 (MQ=255)                                                                                                                                                                                           catcatGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGACGCCTCAACACTGTTCAGCGCCCGCTCCGCAGCCTGGGCGGTCATCTGCACAACATAGTACAAAc                                           >  1:339482/1‑251 (MQ=255)                                                                                                                                                                                              catGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATATGTTACCAGTTCACGGGCGCCGGCAAGCTCAATCGGATCGGCAAACCAGACATCCAAACGCTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGACGCCTCAACACTGTTCAGCGCCCGCTCCGCAGCCTGGGCGGTCATCTGCACAACATAGTACAAACGAt                                        >  1:405918/1‑251 (MQ=255)                                                                                                                                                                                                      ccAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGACGCCTCAACACTGTTCAGCGCCCGCTCCGCAGCCTGGGCGGTCATCTGCACAACATAGTACAAACGATCgcgcgc                                 >  1:35912/1‑250 (MQ=255)                                                                                                                                                                                                               gcgcGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGACGCCTCAACACTGTTCAGCGCCCGCTCCGCAGCCTGGGCGGTCATCTGCACAACATAGTACAATCGATCGCGCGCATCGGGGATg                       <  2:339482/251‑1 (MQ=255)                                                                                                                                                                                                                     tAGTAAAGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCCCGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGACGCCTCAACACTGTTCAGCGCCCGCTCCGCAGCCTGGGCGGTCATCTGCACACCATAGTACAAACGATCGCGCGCATCGGGGATGGCTTcc                 >  2:115737/1‑251 (MQ=255)                                                                                                                                                                                                                        aaaaGCTGGTAAGCGCGGGTACATCTGCTAGAAATTGTCGTGGAGCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTGGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGACGCCTCAACACTGTTCAGCGCCCGCTCCGCAGCCGGGGCGGTCATCTGCACAACATAGTACAAACGATCGCGCGCAGCGGGGATGGCttacgac              <  2:35912/250‑6 (MQ=255)                                                                                                                                                                                                                          aaGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACACGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGACGCCTCAACACTGTTCAGCGCCCGCTCCGCAGCCTGGGCGGTCATCTGCACAACATAGTACAAACGATCGCGCGCATCGGGGATGGCTTCcgccgc            >  1:255419/1‑251 (MQ=255)                                                                                                                                                                                                                           aGCTGGTATGCGCGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTTGGTTAACGCTTTTGCt                                                                                                                                            <  1:332182/122‑1 (MQ=255)                                                                                                                                                                                                                           aGCTGGTATGCGAGGGTACATCTGCTAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCCGTCATCCCAACGTTGGGTTAACGCTTTTGCt                                                                                                                                            >  2:332182/1‑122 (MQ=255)                                                                                                                                                                                                                                    gcgcGGGTACATCTGCTAGAAATTGTTGTGTATCTGTTACCCGTTCACCGGCGTCGCAAAGGTCCAGCGGATCGGCAATCCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCGCCATTTGATCCTGATGCGGTTGTGACGCCTCAACACTGTTCAGCGCCCGCTCCGCAGCCGGGGCGGTCATCTGCACAACATAGTACAAACGATCGCGCGCATCGGGGATGGCTTCCGCCGCTTCGGCAATg  <  2:101355/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           TACCACACCGGCTTTGCTGGTATCGACCTGAGGTCCATTAAGCAAACTCTGGTTTTCACGTTTTGGACGCGACTCCTGTTCCGGGATGTTTTCCAACAGCACCATCAGCAACTGGCGTTCGATCTCCTGAATGACATCCATCATCCGCTTAATGACCTGCCCGGTGAGATCCTGAAAATCCTGCGCCATCATGATTTCCAGCAGTTGCGCGTTAGTAAAGCTGGTATGCGCGGGTACATCTGCCAGAAATTGTCGTGTATCTGTTACCAGTTCACGGGCGTCGGCAAGGTCAATCGGATCGGCAAACCAGTCATCCCAACGTTGGGTTAACGCTTTTGCTGATTTCTCCATTTGATCCTGATGCGGTTGTGACGCCTCAACACTGTTCAGCGCCCGCTCCGCAGCCTGGGCGGTCATCTGCACAACATAGTACAAACGATCGCGCGCATCGGGGATGGCTTCCGCCGCTTCGGCAATG  >  NC_000913/1966441‑1966918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑