Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,839,073	C→T	A109A (GCC→GCT)	ynjD →	putative ABC transporter ATP‑binding protein YnjD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,839,073	0	C	T	100.0%	65.5 / NA	21	A109A (GCC→GCT)	ynjD	putative ABC transporter ATP‑binding protein YnjD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/8);  total (13/8)

CGTCTGGCTGTGGAAAATCCACTCTGTTTTCATGGATGATTGGTGCACTGGCCGAACAGTTTTCTTGTACAGGTGAGCTATGGCTCAATGAGCAACGGATTGACATCCTACCCACCGCACAGCGTCAGATTGGCATTCTTTTTCAGGATGCACTGTTATTTGACCAGTTCAGTGTCGGGCAAAATTTACTGCTGGCGCTACCGGCGACACTTAAAGGGAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGG  >  NC_000913/1838850‑1839296                                                                                                                                                                                                                                |                                                                                                                                                                                                                                cGTCTGGCTGTGGAAAATCCACTCTGTTTTCATGGATGATTGGTGCACTGGCCGAACAGTTTTCTTGTACAGGTGAGCTATGGCTCAATGAGCAACGGATTGACATCCTCCCCACCGCACAGCGTCAGATTGGCATTCTTTTTCAGGATGCACTGTTATTTGACCAGTTCAGTGTCGGGCAAAATTTACTGCTGGCGCTACCGGCGACACTTAAAGGGAATGCTCGACGTAATGCCGTGAATGATGCACtt                                                                                                                                                                                                      <  2:358058/251‑1 (MQ=255)                      ctctGTTTTCATGGATGATTGGTGCACTGGCCGAACAGTTTTCTTGTACAGGTGAGCTATGGCTCAATGAGCAACGGATTGACATCCTACCCACCGCACAGCGTCAGATTGGCATTCTTTTTCAGGATGCACTGTTATTTGACCAGTTCAGTGTCGGGCAAAATTTACTGCTGGCGCTACCGGCGACACTTAAAGGGAATGCTCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAggg                                                                                                                                                                                  >  2:141894/1‑250 (MQ=255)                        ctGTTTTCATGGAGGATTGTTGCACTGGCCGAACAGTTTTCTTGTACAGGTGAGCTATGGCTCAATGAGCAACGGATTGACATCCTCCCCACCGCACAGCGTCAGATTGGCATTCTTTTTCAGGATGCACTGTTATTTGACCAGTTCAGTGTCGGGCAAAATTTACTGCTGGCGCTACCGGCGACACTTAAAGGGAATGCTCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGa                                                                                                                                                                                 <  1:134725/249‑1 (MQ=255)                                                                      cAGGTGAGCTATGGCTCAATGAGCAACGGATTGACATCCTACCCACCGCACAGCGTCAGATTGGCATTCTTTTTCAGGATGCACTGTTATTTGACCAGTTCAGTGTCGGGCAAAATTTACTGCTGGCGCTACCGGCGACACTTAAAGGGAATGCTCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAgcgcgc                                                                                                                                 <  1:278919/251‑1 (MQ=255)                                                                                      cAATGAGCAACGGATTGACATCCTACCCACCGCACAGCGTCAGATTGGCATTCTTTTTCAGGATGCACTGTTATTTGACCAGTTCAGTGTCGGGCAAAATTTACTGCTGGCGCTACCGGCGACACTTAAAGGGAATGCTCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTAcgcg                                                                                                                 >  1:353546/1‑251 (MQ=255)                                                                                          gAGCAACGGATTGACATCCTACCCACAGCACAGCGCCAGATTGGAATTCTTTTTCAGGATGCACTGTTATTTGACAAGTTCAGTGTCGGGCAAAATTTACTGCTGGAGCTACCGGCGACCCTTAAAGGGAATGCTCGAAGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTCTGTCTGGCGGTCAGCGAACGCGCGTTGCTCTACTCCGCGACCt                                                                                                             >  2:52936/1‑251 (MQ=255)                                                                                             cAACGGATTGACATCCTACCCACCGCACAGCGTCAGATTGGCATTCTTTTTCAGGATGCACTGTTATTTGACCAGTTCAGTGTCGGGCAAAATTTACTGCTGGCGCTACCGGCGACACTTAAAGGGAATGCTCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTtct                                                                                                          >  1:250482/1‑251 (MQ=255)                                                                                                  gATTGACATCCTACCCACCGCACAGCGTCAGATTGGCATTCTTTTTCAGGATGCACTGTTATTTGACCAGTTCAGTGTCGGGCAAAATTTACTGCTGGCGCTACCGGCGACACTTAAAGGGAATGCTCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTTCTCGccc                                                                                                     >  1:333399/1‑251 (MQ=255)                                                                                                                        cagtttCAGATTGGCATTCTTTTTCAGGATGCACGGTTAATTGACCAGTGCAGGGTCGGGCAAAATGTACTGCTGGCGTTACCGGGGACACTTAAAGGGAATGCTTGACGTAAAGCCGTGAATGATGCGCTTGGGCGTGCAGGTCTTGAGGTAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGGCTGCGAGCGCGCGTGGCTCTTCTACGAGCATTTCTCGCCCATCCAACAGCGTTCCTTCTGGa                                                                               <  2:250821/246‑1 (MQ=255)                                                                                                                           cGTCAGATTGGCATTCTTTTTCAGGATGCACTGTTATTTGACCAGTTCAGTGTCGGGCAAAATTTACTGCTGGCGCTACCGGCGACACTTAAAGGGAATGCTCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATg                                                                             >  1:35877/1‑250 (MQ=255)                                                                                                                              cAGATTGGCATTCTTTTTCAGGATGCACTGTTATTTGACCAGTTCAGTGTCGGGCAAAATTTACTGCTGGCGCTACCGGCGACACTTAAAGGGAATGCTCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGc                                                                          <  2:10402/250‑1 (MQ=255)                                                                                                                                 aTTGGCATTCTTTTTCAGGATGCACTGTTATTTGACCAGTTCAGTGTAGGGCAAAATTTACTGCTGGCGCTACCGGCGACACTTAACGGGAATGCTCGAAGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGAGCGTTGCTCTACTACGCGCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGCGCCAtt                                                                      >  1:274425/1‑251 (MQ=255)                                                                                                                                               gcAGGATGCACTGTTATTTGACCAGTTCAGTGTCGGGCAAAATTTACTGCTGGCGCTACCGGCGACACTTAAAGGGAATGCTCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGAt                                                         <  1:338921/249‑1 (MQ=255)                                                                                                                                                  ggATGCACTGTTATTTGACCAGTTCAGTGTCGGGCAAAATTTACTGCTGGCGCTACCGGCGACACTTAAAGGGAATGCTCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTgg                                                     <  1:225551/251‑1 (MQ=255)                                                                                                                                                             tATTTGACCAGTTCAGTGTCGGGCAAAATTTACTGATGGCGCTACAGGCGACACTTAAAGGGAATGCTCGACGTACTGCCGTGAATGATGCACTTGAGCGTTCAGGCATTGAGGGAGATTTCCCTCAGGATACTGCCACTTTGTCTGGCGGTCAGCGCGCGCGCGTTGCTCTACTACGCGCCCTTCTCGCCCAACCAAAAGAGTTCCTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCAGCGCGAt                                          >  1:382723/1‑251 (MQ=255)                                                                                                                                                                                cGGGCAAAATTTACTGCTGGCGCTACCGGCGACACTTAAAGGGAATGCTCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTTATCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGCTAATTTTCGCCCGTGGgtgt                       >  2:177534/1‑251 (MQ=255)                                                                                                                                                                                       aaTTTACTGCTGGCGCTACCGGCGACACTTAAAGGGAATGCTCGACGTAATGCCGTGAATGATGCACTTGAGCGTCCAGGCCTTGAGGGAGCTTTACATCAGGCTCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTTCTCGCCCAACCAAACGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCg                 >  1:246144/1‑250 (MQ=255)                                                                                                                                                                                         tttACTGCTGGCGCTACCGGCGACACTTAAAGGGAATGCTCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGa                >  1:62467/1‑249 (MQ=255)                                                                                                                                                                                               gctgGCGCTACCGGCGACACTTAAAGGGAATGCTCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTcgcg        <  2:333399/251‑1 (MQ=255)                                                                                                                                                                                                  ggCGCTACCGGCGACACTTAAAGGGAATGCTCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGccc     >  1:161516/1‑251 (MQ=255)                                                                                                                                                                                                     gcTACCGGCGACACTTAAAGGGAATGCTCGACGTCATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTgg  >  1:110242/1‑251 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                CGTCTGGCTGTGGAAAATCCACTCTGTTTTCATGGATGATTGGTGCACTGGCCGAACAGTTTTCTTGTACAGGTGAGCTATGGCTCAATGAGCAACGGATTGACATCCTACCCACCGCACAGCGTCAGATTGGCATTCTTTTTCAGGATGCACTGTTATTTGACCAGTTCAGTGTCGGGCAAAATTTACTGCTGGCGCTACCGGCGACACTTAAAGGGAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTTCTCGCCCAACCAAAAGCGTTACTCCTGGATGAGCCATTCAGCCGTCTTGATGTGGCTCTGCGCGATAATTTTCGCCAGTGGGTGTTCAGCGAAGTTCGCGCCCTGG  >  NC_000913/1838850‑1839296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑