Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,791,414	T→A	T203S (ACG→TCG)	rflP ←	anti‑FlhDC factor RflP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,791,414	0	T	A	100.0%	41.9 / NA	14	T203S (ACG→TCG)	rflP	anti‑FlhDC factor RflP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (5/9);  total (5/9)

GCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACGGTTACTCTCGTCTTAAACACCAGCAAACAGAAGGGTTATCGCTGAACCAACGTCGTTATCTGATTTATGGGAACGGCAGGCCACAGGCATCCCTGGAGAGCATGGAAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACC  >  NC_000913/1791189‑1791636                                                                                                                                                                                                                                  |                                                                                                                                                                                                                               gCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACGGTTACTCTCGTCTTAAACACCAGCAAACAGAAGGGTTATCGCTGAACCAACGTCGTTATCTGATTTATGGGAACGGCAGGCCACAGGCATCCCTGGAGAGCATGGAAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGAATCAATGCCTCCCGCAATGATGCa                                                                                                                                                                                                        <  1:87964/250‑1 (MQ=255)                    aTTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACGGTTACTCTCGTCTTAAACACCAGCAAACAGAAGGGTTATCGCTGAACCAACGTCGTTATCTGATTTATGGGAACGGCAGGCCACAGGCATCCCTGGAGAGCATGGAAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGAATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAAttt                                                                                                                                                                                    <  1:188033/251‑1 (MQ=255)                           gcgcCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACGGTTACTCTCGTCTTAAACACCAGCAAACAGAAGGGTTATCGCTGAACCAACGTCGTTATCTGATTTATGGGAACGGCAGGCCACAGGCATCCCTGGAGAGCATGGAAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGAATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTg                                                                                                                                                                             <  1:122497/251‑1 (MQ=255)                                 gCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACGGTTACTCTCGTCTTAAACACCAGCAAACAGAAGGGTTATCGCTGAACCAACGTCGTTATCTGATTTATGGGAACGGCAGGCCACAGGCATCCCTGGAGAGCATGGAAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGAATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGTATCGc                                                                                                                                                                       >  2:230949/1‑251 (MQ=255)                                            aaGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACGGTTACTCTCGTCTTAAACGCCAGCAAACAGAAGGGTTATCGCTTAACCAACGTCGTTATCTGATTTATGGGAACGGCAGGCCACAGGCATCCCTGGAGATCATGGAAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGAATCAATGCCTCCCg                                                                                                                                                                                                                  <  2:335064/197‑1 (MQ=255)                                            aaGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACGGATACCCTCGTCTTAAACACCAGCAAACAGAAGGGTTATCGGTGAACCAACGTCGTTATCTGATTTATGGGAACGGCAGGCCACCGGCATCCCTGGAGAGCATGGAAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGAATCAATGCCTCCCg                                                                                                                                                                                                                  >  1:335064/1‑197 (MQ=255)                                              gACAGGGACATAGATCCTCCTGTTTGATAGTGTAGACGGTTACTCTCGTCTTAAACACCAGCAAACAGAAGGGTTATCGCTGAACCAACGTCGTTATCTGATTTATGGGAACGGCAGGCCACAGGCATCCCTGGAGAGCATGGAAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGAATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGCAATTTGCGCCTGGATCGAACGAATAAACGGt                                                                                                                                                          >  2:150595/1‑251 (MQ=255)                                                      cATAGATCCTCCTGTTTGATAGTGTAGACGGTTACTCTCGTCTTAAACACCAGCAAACAGAAGGGTTATCGCTGAACCAACGTCGTTATCTGATTTATGGGAACGGCAGGCCACAGGCATCCCTGGAGAGCATGGAAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGAATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGa                                                                                                                                                  <  2:317237/251‑1 (MQ=255)                                                        tAGATCCTCCTGTTTGATAGAGTAGACGGCTACTCTCGTCTTAAACACCAGCAAACACAAGGGTTATCGCTGAACCAACGACGTTATCTGATTTATGGGAACGGCAGGCCACAGGACTCCCTGGAGAGCATGGTAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGAATCAATGCCTCCCGCACTGATGCAATTGCAACAAGGGGAAATTTGCGCCTGCATCGACCGAATAAACGGTTCGAAGTAGc                                                                                                                                                >  2:331152/1‑251 (MQ=255)                                                                                                   aaCACCAGCAAACAGAAGGGGTATCGCTGAACCAACGTCGTTATCTGATTTATGGGAACGGCAGGCCACAGGCATCCCTGGAGAGCATTGAAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGAATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGAc                                                                                                     <  2:263744/251‑1 (MQ=255)                                                                                                                           cGCTGAACCAACGTCGTTATCTGATTTATGGGAACGGCAGGCCACAGGCATCCCTGGAGAGCATGGAAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGAATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAAc                                                                             >  2:136588/1‑251 (MQ=255)                                                                                                                                                            aCGGCAGGCCACAGGCATCCCTGGAGAGCATGGAAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGAATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAAtt                                            <  1:230949/251‑1 (MQ=255)                                                                                                                                                                                  ggAGAGCATGGAAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGAATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTTAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATaca                        <  1:59046/249‑1 (MQ=255)                                                                                                                                                                                                      ggggTAATCTGTGCCAATATTTCTGCCGAATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGAcc  <  1:6256/251‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                               GCTGTATAGGTTTCTGGCAATTCATCGCGCCAGCGGGTAACAAAAGACAGGGTCATAGATCCTCCTGTTTGATAGTGTAGACGGTTACTCTCGTCTTAAACACCAGCAAACAGAAGGGTTATCGCTGAACCAACGTCGTTATCTGATTTATGGGAACGGCAGGCCACAGGCATCCCTGGAGAGCATGGAAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACC  >  NC_000913/1791189‑1791636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑