Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,414,813	G→A	R858R (CGC→CGT)	recE ←	exonuclease VIII, ds DNA exonuclease, 5' ‑‑> 3' specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,414,813	0	G	A	100.0%	55.6 / NA	18	R858R (CGC→CGT)	recE	exonuclease VIII, ds DNA exonuclease, 5' ‑‑> 3' specific
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/9);  total (9/9)

AGTTTCCTAACGCCGGAACTTTACGAATTTCTGTGGTGGCGATACGGATCATACGTTCAGCCGTCATATGGCGTGGAAGAGCTGCTGCCAGTTGCTCTTTCATTGATGGCTGGTTAATAAAACTAATCACGTCGCTATTTTTAACTGCTGCTGGTGCACGGTTTCCCTGAGTTTTTTGCAGATCGGCTTTTGCGATTGGTGGTTGCTTAGTCATTTGCATATTCCTTAGCCCAGCGGGGCAGTGATAATGTCTTAATAGCTGGCCATTCATCGGTATTCAGGCAGTCAGACAGGGTTCGCAGATTGCGGTGATATTCCTGTTGACCTGCCAGTTTTGCTTCTTCGCCCATCATGAAAATTTCAACCGGATAACGTCCGCATTCAATAGTTGTGCTGGCAACCAGAAAAACGAAAGTTGGCTGCACTCCAAACTGTGCTTCATAACCGTCACTGTAGAATGCATCCTGAACGTGATAGCGG  >  NC_000913/1414580‑1415059                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       aGTTTCATAACGCCGGAACTTTACGAATTACTGTGGTGGCGTTACGTATTATCCGTTCATCCGTCATATGGCGTGGAATAGCTGCTGCCAGTTGCTCTTTCATTGATGGCTGTTTAATAAAACTAATCACGTCGCTATTTTTAACTGCTGCTGGTGCACGGTTTCCCTGAGTTTGTTGCAGATCGGCTTTTGCGATTGGTTGTTGCTTAGTCATGTGCATATTCCTTATTCCAACGGGGCAGTGATAATGt                                                                                                                                                                                                                                       <  2:199378/251‑1 (MQ=255)                          caTTTCGGTGGTGGCGATACGGATCCTACGTTGAGCCGGCATATGGTGTGGAGGAGCTGCTGCCAGTTGCTCTTTCATTGATGGCTGGTTAATAAGACTAATCACGTCGCTATTTTTAACTGATGCGGGTGCACGGATTCCCTGAGTTTTTTGCAGATAGGCTTTTGCGATTGGTAGTTGCTTCGTAATTTGCATGTTCCTTAGCCAAACGGGGCAGGGGTAATGACTTAATAGCTGGTCATTCATCggc                                                                                                                                                                                                               >  2:352848/2‑249 (MQ=255)                            tttCTGTGGTGGCGATACGGATCATACGTTCAGCCGTCATATGGCGTGGAAGAGCTGCTGCCAGTTGCTCTTTCATTGATGGCTGGTTAATAAAACTAATCACGTCGCTATTTTTAACTGCTGCTGGTGCACGGTTTCCCTGAGTTTTTTGCAGATCGGCTTTTGCGATTGGTGGTTGCTTAGTCATTTGCATATTCCTTAGCCCAACGGGGCAGTGATAATGTCTTAATAGCTGGCCATTCATCGGTAtt                                                                                                                                                                                                            >  2:172020/1‑251 (MQ=255)                                                            gCCGTCATATGGCGTGGAAGAGCTGCTGCCAGTTGCTCTTTCATTGATGGCTGGTTAATAAAACTAATCACGTCGCTATTTTTAACTGCTGCTGGTGCACGGTTTCCCTGAGTTTTTTGCAGATCGGCTTTTGCGATTGGTGGTTGCTTAGTCATTTGCATATTCCTTAGCCCAACGGGGCAGTGATAATGTCTTAATAGCTGGCCATTCATCGGTATTCAGGCAGTCAGACAGGGTTCGCAGATTGCGGt                                                                                                                                                                            <  2:357173/251‑1 (MQ=255)                                                              cGTCATATGGCGTGGAAGAGCTGCTGCCAGTTGCTCTTTCATTGATGGCTGGTTAATAAAACTAATCACGTCGCTATTTTTAACTGCTGCTGGTGCACGGTTTCCCTGAGTTTTTTGCAGATCGGCTTTTGCGATTGGTGGTTGCTTAGTCATTTGCATATTCCTTAGCCCAACGGGGCAGTGATAATGTCTTAATAGCTGGCCATTCATCGGTATTCAGGCAGTCAGACAGGGTTCGCAGATTGCGGTGa                                                                                                                                                                          <  1:172020/251‑1 (MQ=255)                                                                         gTGGAAGAGCTGCTGCCAGTTGCTCTTTCATTGATGGCTGGTTAATAAAACTAATCACGTCGCTATTTTTAACTGCTGCTGGTGCACGGTTTCCCTGAGTTTTTTGCAGATCGGCTTTTGCGATTGGTGGTTGCTTAGTCATTTGCATATTCCTTAGCCCAACGGGGCAGTGATAATGTCTTAATAGCTGGCCATTCATCGGTATTCAGGCAGTCAGACAGGGTTCGCAGATTGCGGTGATATTCCTGTTg                                                                                                                                                               >  2:206993/1‑251 (MQ=255)                                                                                 gctgctGCCAGTTGCTCTTTCATTGATGGCTGGTTAATAAAACTAATCACGTCGCTATTTTTAACTGCTGCTGGTGCACGGTTTCCCTGAGTTTTTTGCAGATCGGCTTTTGCGCTTGGTGGTTGCTTAGTCATTTGCATATTCCTTAGCCCAACGGGGCAGTGATAATGTCTTAATAGCTGGCCATTCATCGGTATTCAGGCAGTCAGACAGGGTTCGCAGATTGCGGTGATATTCCTGTTGACCTGcc                                                                                                                                                        >  1:192905/1‑250 (MQ=255)                                                                                            ttGCTCTTTCATTGATGGCTGGTTAATAAAACTAATCACGTCGCTATTTTTATCTGCTGCTGGTGCACGGTTTCCCTGAGTTTTTTGCAGATCGGCTTTTGCGATTGGTGGTTGCTTAGTCATTTGCATATTCCTTAGCCCAACGGGGCAGTGATAATGTCTTAATAGCTGGCCATTCATCGGTATTCAGGCAGTCAGACAGGGTTCGCAGATTGCGGTGATATTCCTGTTGACCTGCCAGTTTTGcttct                                                                                                                                            >  2:155198/1‑251 (MQ=255)                                                                                                           tGGCTGGTTAATAAAACTAATCACGTCGCTATTTTTAACTGCTGCTGGTGCACGGTTTCCCTGAGTTTTTTGCAGATCGGCTTTTGCGATTGGTGGTTGCTTAGTCATTTGCATATTCCTTAGCCCAACGGGGCAGTGATAATGTCTTAATAGCTGGCCATTCATCGGTATTCAGGCAGTCAGACAGGGTTCGCAGATTGCGGTGATATTCCTGTTGACCTGCCAGTTTTGCTTCTTCGCCCATCATGaa                                                                                                                              >  2:182262/1‑250 (MQ=255)                                                                                                                                 aCGTCGCTATTTTTAACTGCTGCTGGTGCACGGTTTCCCTGAGTTTTTTGCAGATCGGCTTTTGCGATTGGTGGTTGCTTAGTCATTTGCATATTCCTTAGCCCAACGGGGCAGTGATAATGTCTTAATAGCTGGCCATTCATCGGTATTCAGGCAGTCAGACAGGGTTCGCAGATTGCGGTGATATTCCTGTTGACCTGCCAGTTTTGCTTCTTCGCCCATCATGAAAATTTCAACCGGATAACGTCCGc                                                                                                       <  1:214628/251‑1 (MQ=255)                                                                                                                                             ttAACTGCTGCTGGTGCACGGTTTCCCTGAGTTTTTTGCAGATCGGCTTTTGCGATTGGTGGTTGCTTAGTCATTTGCATATTCCTTAGCCCAACGGGGCAGTGATAATGTCTTAATAGCTGGCCATTCATCGGTATTCAGGCAGTCAGACAGGGTTCGCAGATTGCGGTGATATTCCTGTTGACCTGCCAGTTTTGCTTCTTCGCCCATCATGAAAATTTCAACCGGATAACGTCCGCATTCAATAGtt                                                                                            <  1:328034/250‑1 (MQ=255)                                                                                                                                                                        tGAGTTTTTTGCAGATCGGCTTTTGCGATTGGTGGTTGCTTAGTCATTTGCATATTCCTTAGCCCAACGGGGCAGTGATAATGTCTTAATAGCTGGCCATTCATCGGTATTCAGGCAGTCAGACAGGGTTCGCAGATTGCGGTGATATTCCTGTTGACCTGCCAGTTTTGCTTCTTCGCCCATCATGAAAATTTCAACCGGATAACGTCCGCATTCAATAGTTGTGCTGGCAACCAGAAAAACGAAAGTTg                                                                >  1:347245/1‑251 (MQ=255)                                                                                                                                                                           gTTTTTTGCAGTTCGGCTTTTGCGTTTGGTGGTTGCTTAGTCATTTGCATATTCCTTAGCCCAACGGGGCAGTGATAATGTCTTAATAGCTGGCCATTCATCGGTATTCAGGCAGTCAGACAGGGTTCGCAGATTGCGGTGATATTCCTGTTGACCTTCCAGTTTTTCTTCTTCGCCCATCATGTAACTTTCAACCGGATAACGTCCGCATTCTATAGTTGTGCTTGCAACCAGAAAAACGAAAGTTGGCt                                                             >  2:101463/1‑251 (MQ=255)                                                                                                                                                                             tttttGCAGATCGGCTTTTGCTATTGTGGGTTGCTTAGTCAGTTGCATATTCCTTAGCCCAACGGGGCAGTGATAATGTTTTAATAGCTGGCCATTTATCGGTATTCAGGCAGTCATACAGGGTTCGCAGATTGCGGTGATATTCCTGTTGACCTGCCAGTTTTGCTTCTTCGCCCATCATGAAAATTTCAACCGGATAACGTTCGCATTCAATAGTTGTGCTGGCAACCCGAAAAACGAAAGTTAGCTg                                                            <  2:52848/250‑1 (MQ=255)                                                                                                                                                                             tttttGCAGATCGGCTTTTGCGATTGGTGGTTGCTTAGTCATTTGCATATTCCTTAGCCCAACGGGGCAGTGATCATGTCTTAATAGCTGGCCATTCATCGGTATTCAGGCAGTCAGACAGGGTTCGCAGATTGCGGTGATATTCCTGTTGTCATGCAAGTTTTGATTCTTCGCCCATCATGAAAATTTCAACCGGATAACGTCCGCATTCAATAGTTGTGCTGTCAACCAGAAAAACGAAAGTTGGCTGc                                                           >  2:416407/1‑251 (MQ=255)                                                                                                                                                                                             tttGCGATTGGTGGTTGCTTAGTCATTTGCATATTCCTTAGCCCAACGGGGCAGTGATAATGTCTTAATAGCTGGCCATTCATCGGTATTCAGGCAGTCAGACAGGGTTCGCAGATTGCGGTGATATTCCTGTTGACCTGCCAGTTTTGCTTCTTCGCCCATCATGAAAATTTCAACCGGATAACGTCCGCATTCAATAGTTGTGCTGGCAACCAGAAAAACGAAAGTTGGCTGCACTCCAAACTGTGc                                             <  1:206993/249‑1 (MQ=255)                                                                                                                                                                                                                             atTCCTTAGCCCAACGGGGCAGTGATAATGTCTTAATAGCTGGCCATTCATCGGTATTCAGGCAGTCAGACAGGGTTCGCAGATTGCGGTGATATTCCTGTTGACCTGCCAGTTTTGCTTCTTCGCCCATCATGAAAATTTCAACCGGATAACGTCCGCATTCAATAGTTGTGCTGGCAACCAGAAAAACGAAAGTTGGCTGCACTCCAAACTGTGCTTCATAACCGTCACTGTATAATGCATCCTGAAc            <  2:64691/250‑1 (MQ=255)                                                                                                                                                                                                                                      cccAACGGGGCAGTGATAATGTCTTAATAGCTGGCCATTCATCGGTATTCAGGCAGTCAGACAGGGTTCGCAGATTGCGGTGATATTCCTGTTGACCTGCCAGTTTTGCTTCTTCGCCCATCATGAAAATTTCAACCGGATAACGTCCGCATTCAATAGTTGTGCTGGCAACCAGAAAAACGAAAGTTGGCTGCACTCCAAACTGTGCTTCATAACCGTCACTGTAGAATGCATCCTGAACGTGATAGCgg  <  1:182262/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       AGTTTCCTAACGCCGGAACTTTACGAATTTCTGTGGTGGCGATACGGATCATACGTTCAGCCGTCATATGGCGTGGAAGAGCTGCTGCCAGTTGCTCTTTCATTGATGGCTGGTTAATAAAACTAATCACGTCGCTATTTTTAACTGCTGCTGGTGCACGGTTTCCCTGAGTTTTTTGCAGATCGGCTTTTGCGATTGGTGGTTGCTTAGTCATTTGCATATTCCTTAGCCCAGCGGGGCAGTGATAATGTCTTAATAGCTGGCCATTCATCGGTATTCAGGCAGTCAGACAGGGTTCGCAGATTGCGGTGATATTCCTGTTGACCTGCCAGTTTTGCTTCTTCGCCCATCATGAAAATTTCAACCGGATAACGTCCGCATTCAATAGTTGTGCTGGCAACCAGAAAAACGAAAGTTGGCTGCACTCCAAACTGTGCTTCATAACCGTCACTGTAGAATGCATCCTGAACGTGATAGCGG  >  NC_000913/1414580‑1415059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑