| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,259,717 | C→T | A363T (GCC→ACC) | dauA ← | aerobic C4‑dicarboxylate transporter DauA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,259,717 | 0 | C | T | 95.7% | 63.1 / ‑6.5 | 23 | A363T (GCC→ACC) | dauA | aerobic C4‑dicarboxylate transporter DauA |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (10/12); total (11/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
CGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATAACCATATCAAACAACACGGTCAGCGACATGCACAGCAGCATGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGC > NC_000913/1259473‑1259963 | cGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATAACCATATCAAACAACACGGTCAGCGACATGCACAGCAGCATGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGTAAGAAt < 1:261556/251‑1 (MQ=255)cGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATAACCATAGCAAACAACACGGTCAGCGACATGCACAGCAGCATGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGTAAGAAt < 2:331019/251‑1 (MQ=255) cGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATAACCATATCAAACAACACGGTCAGCGACATGCACAGCAGCATGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGTAAGAATAAcc < 1:130783/251‑1 (MQ=255) aaaCAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATAACCATATCAAACAACACGGTCAGCGACATGCACAGCAGCATGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGTAAGAATAACCAGAATAGAGTgg < 2:320505/250‑1 (MQ=255) aaCCATATCAAACAACACGGTCAGCGACATGCACAGCAGCATGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGTAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGtt > 1:216158/1‑249 (MQ=255) ccATATCAAACAACACGGTCAGCGACATGCACAGCAGCATGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGTAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCg > 2:289241/1‑251 (MQ=255) tCAAACAACACGGTCAGCGACATGCACAGCAGCATGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGTAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGc > 1:4697/1‑248 (MQ=255) ggTCAGCGACATGCACAGCAGCATGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGTAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATgg > 2:120280/1‑251 (MQ=255) gACGATGATGTCATCTTCCGGCGCATGACGCAGCAAGCCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGTAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATAccacca < 1:123397/250‑1 (MQ=255) cGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGTAAGAATAACCAGAATAGAGTGGATCACCGCCGCGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAatat > 1:340072/1‑251 (MQ=255) ggCGAATGACGCAGCAAGTCGACCACTTTGCGCGCTTCACTCATGTTCCACGCCACAATCAACGGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGTAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATAtt < 2:4697/251‑1 (MQ=255) cgcATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGTAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTcc > 2:245335/1‑251 (MQ=255) cGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGTAAGAATAACCAGAAGAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTccc < 1:197904/242‑1 (MQ=255) cGACCACTTTCTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGTAAGAATAACCCGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGCCGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTcccagatcggac > 2:197904/1‑242 (MQ=255) gggctcgccacccctgtcccACACCACCATCAACAGCAGTGCTGCATTGGCGGAAAGCGACAGCCAGAGGAGCAGCGGCGCCAGTACCAGGAGGGTAAGAATAACAAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGTGCGATGGCAGCTGGAGCTTTAATACCACCAAAGAACGGAGCGATAAGATTCCCCAGTCCCTGTCCAAGCAGTTCGCTGt < 2:203382/233‑1 (MQ=255) caccGCCACCATCAACAGCGGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGTAAGAATAACCAGAAGAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCggcc < 2:177010/248‑4 (MQ=255) ccaTCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGTAAGAATAACCAGAAAAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATa < 1:5109/251‑1 (MQ=255) cagcaagccTGCCATGGCGGACAGCGGCCGCCAGGAGAGCAGCCGTGCCAGTACAAGCAGGGCAAGACTAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTATCGGAAGAACGCGCGGTGGCAGCTGTAGCGGTAATACCACCAACGAACTGAGCGATAATATTCCCCAGTCCCTGGACAAACAGATCGCTGCTCGCCTTGCGTTTCGTCCCGATCaaccccaccc > 2:177393/9‑242 (MQ=255) ggAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGTCAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCgcagagc > 1:166358/1‑250 (MQ=255) gagCAGCGGTGCCAGTACCAGCAGGGTAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTGCGCCGa < 1:358527/251‑1 (MQ=255) gcGGTGCCAGTCCCAGCAGGGTAAGAATAACCAGAATAGGGGGGATCCCCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTGCGCCGAGCAtt < 2:216158/251‑1 (MQ=255) aGTACCAGCAGGGTAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTg > 1:239953/1‑250 (MQ=255) agGGTAAGAATAACCAGAATAGAGTGGATAACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCgc > 1:89638/1‑251 (MQ=255) | CGTGCGATACGACGCATAAACAGCAGCGATGCCAGCACGATCCCCACGCTGATGGCAATAACCATATCAAACAACACGGTCAGCGACATGCACAGCAGCATGACGATGATGTCATCTTTCGGCGCATGACGCAGCAAGTCGACCACTTTGTGCGCTTCACTCATGTTCCACGCCACCATCAACAGCAGGGCTGCCATGGCGGAAAGCGGCAGCCAGGAGAGCAGCGGTGCCAGTACCAGCAGGGCAAGAATAACCAGAATAGAGTGGATCACCGCCGAGATAGGGGACGTTGCCCCGGCACGGACGTTAGCGGCAGAACGCGCGATGGCAGCTGTAGCGGTAATACCACCAAAGAACGGAGCGATAATATTCCCCAGTCCCTGTCCAACCAGTTCGCTGTTCGCCTTGTGTTTCGTCCCGGTCATACCATCCAGCACCACGGCGCAGAGCAGAGATTCGATTGCGCCGAGCATTGCCATTGAGAATGCCGC > NC_000913/1259473‑1259963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |