Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,049,827	T→G	intergenic (‑83/‑6)	gfcA ← / → insA4	threonine‑rich inner membrane protein GfcA/IS1 family protein InsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,049,827	0	T	G	100.0%	87.2 / NA	31	intergenic (‑83/‑6)	gfcA/insA4	threonine‑rich inner membrane protein GfcA/IS1 family protein InsA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/14);  total (17/14)

GTAGTAGCAGTCGTGGTTGCGCCGGTAGTGGTCGTTGTGGTGCCAGTAGTACCCGTCGTTGCCTCGGTCCCGTTTGTTGCTTCAGGGGCAGCAAACGCAGCTGGCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGT  >  NC_000913/1049593‑1050023                                                                                                                                                                                                                                           |                                                                                                                                                                                                     gtggtagCCGTCGTGGTTGCTCCGGGTGTCGTCGTTGCGGTGCCAGTAGTACCCGTCGTTGCCTCGGTCCCGTTTGTTGCGTCAGGGGCAGCAAACGCAGCTGGCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTGTGAGGTGCTCCAGTTTCTTCTGt                                                                                                                                                                                      <  2:252903/248‑1 (MQ=255)   agtagCAGTCGTGGTTGCGCCGGTAGGGGCCGTTGTGGTGCCAGTAGTACCCGTCGTTGCCTCGGTCCCGTTTGTTGCTTCAGGGGCAGCAAACGCAGCTGGCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATACCTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGttt                                                                                                                                                                                    >  2:287872/1‑251 (MQ=255)      agCAGTCGTGTTTGCGCCGGTAGTGGTCGTTGTGGTGCCAGTAGTACCCGTCGTTGCCTCGGTCCCGTTTGTTGCTTCAGGGGCAGCAAACGCAGCTGGCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTa                                                                                                                                                                                 <  2:225937/251‑1 (MQ=255)        cAGTCGTGGTTGCGCCGGTAGTGGTCGTTGTGGTGCCAGTAGTACCCGTCGTTGCCTCGGTCCCGTTTGTTGCTTCAGGGGCAGCAAACGCAGATGGCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTCTGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATc                                                                                                                                                                               >  2:409816/1‑251 (MQ=255)            cgtggttgtggcgggatggttcgatgtgGTGCCGGTAGTACCCGTCGTTGCCTCGTTCCCGTTTGTTGCTTCAGGGGCAGCAAACGCCGCTGGCGGCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTTTTTCATAGCGTTTCCTTTATTCATCGCATGGACAATCCGGGTGAGGCTTCTAACTTACTGATTTAGTGTAGGATGGTGTTGTTGAGGTGCTCCAGTGGCTTCTTTTTCTATCAGcg                                                                                                                                                                           <  2:26259/227‑2 (MQ=255)                           aGTGGTCGTTGTGGTGCCAGTAGTACCCGTCGTTTCCTCGGTCCCGTTTGTTGCTTCCGGGGCAGCAAACGCCGCTGGCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTc                                                                                                                                                              >  2:74167/1‑249 (MQ=255)                                                       gtcgtTGCCTCGGTCCCGTTTGTTGCTTCAGGGGCAGCAAACGCAGCTGGAGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGGTTTAGTGAATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTACTGTTCAGCTCCTGACGGGGTGGTGCGTAACGGCa                                                                                                                                 >  1:274109/1‑250 (MQ=255)                                                          gtTGCCTCGGTCCCGTTTGTTGCTTCAGGGGCAGCAAACGCAGCTGGCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCaaa                                                                                                                               >  2:224885/1‑249 (MQ=255)                                                                            gttgCTTCAGGGGCAGCAAACGCAGCTGGCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGGTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAgcg                                                                                                           <  2:139961/251‑1 (MQ=255)                                                                                                          gtcgtcAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATg                                                                             <  1:127569/251‑1 (MQ=255)                                                                                                                 aCATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACACTACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTg                                                                      >  2:185308/1‑251 (MQ=255)                                                                                                                          ccATAAGGATTGCAGAAAGCTTGTGTTTCATAACGTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACt                                                             <  1:267911/251‑1 (MQ=255)                                                                                                                              aaGGATTGCAGAAAGCTTGTGTGTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACGGCCGTAAAACATGGCAACTGCAGTTCACTTaca                                                         <  2:347484/251‑1 (MQ=255)                                                                                                                                               tgtgTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCg                                          <  1:55827/249‑1 (MQ=255)                                                                                                                                                 tgtTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTAc                                      >  1:335396/1‑251 (MQ=255)                                                                                                                                                      cATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAAATTACTGATTTAGTGTATGATGGCGCTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAAAGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAACCATGGCAACTGCAGTTCACTTAAACCGCTTATCAACACGGTACGCAcc                                 >  1:179710/1‑251 (MQ=14)                                                                                                                                                        tAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCAcc                                 >  1:196511/1‑249 (MQ=255)                                                                                                                                                             tttCCTTTATTCATCGCATGGACAATACGGGTTATGCTGCCAACGTACTGATTTAGTGTATGAGGGTGTTTGTGAGGTGCTCCAGGGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTAc                                                                                                                                                        <  2:408395/125‑1 (MQ=17)                                                                                                                                                             tttCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTAc                                                                                                                                                        >  1:408395/1‑125 (MQ=255)                                                                                                                                                                  tttcttCATCGCATGGAAAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCACTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACCTCAGCGCTATCTCTGCTATCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACACGAAAATCATTg                     >  2:189174/5‑251 (MQ=11)                                                                                                                                                                   ttATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCggagatcggaagagcacaacgtc                    >  1:62218/1‑230 (MQ=255)                                                                                                                                                                   ttATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACAGCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCgg                                         <  2:62218/230‑1 (MQ=255)                                                                                                                                                                   ttATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGGGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCAtt                      <  2:196511/249‑1 (MQ=255)                                                                                                                                                                       tCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGatat                 <  2:335396/250‑1 (MQ=37)                                                                                                                                                                         aTCGCATGGACAATACGGGCGATGCTGCCAACTTACTGATTTCGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGc              >  2:66756/1‑251 (MQ=21)                                                                                                                                                                         aTCGCAGGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGc              <  1:287872/251‑1 (MQ=18)                                                                                                                                                                             cATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCAt           <  1:71317/250‑1 (MQ=18)                                                                                                                                                                             cATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCAt           >  2:71317/1‑250 (MQ=18)                                                                                                                                                                                 gACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATg      >  1:91312/1‑251 (MQ=14)                                                                                                                                                                                    aaTACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCg   >  1:357457/1‑251 (MQ=11)                                                                                                                                                                                     aTACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAAAGGCGt  >  2:129018/1‑251 (MQ=11)                                                                                                                                                                                                                                           |                                                                                                                                                                                                     GTAGTAGCAGTCGTGGTTGCGCCGGTAGTGGTCGTTGTGGTGCCAGTAGTACCCGTCGTTGCCTCGGTCCCGTTTGTTGCTTCAGGGGCAGCAAACGCAGCTGGCGTCGTCAACATGAAGGCCATAAGGATTGCAGAAAGCTTGTGTTTCATAACTTTTCCTTTATTCATCGCATGGACAATACGGGTGATGCTGCCAACTTACTGATTTAGTGTATGATGGTGTTTTTGAGGTTCTCCAGTGGCTTCTGTTTCTATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGT  >  NC_000913/1049593‑1050023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑