Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	934,781	C→A	P520T (CCG→ACG)	ftsK →	cell division DNA translocase FtsK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	934,781	0	C	A	100.0%	109.1 / NA	31	P520T (CCG→ACG)	ftsK	cell division DNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/9);  total (22/9)

AATCCACTTTTGCTCCACAGTCTACATACCAGACTGAGCAAACTTATCAGCAGCCAGCCGCTCAGGAGCCGTTGTAC‑CAACAGCCGCAACCCGTTGAACAGCAGCCTGTTGTGGAGCCTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCTGGCATCTGGCGTGAAAAAAGCGACACTGGCGACGGGGGCTGCCGCAACCGTTGCCGCGCCAGTCTTCAGTCTGGCAAATAGCGGTGGACCGCGTCCTCAGGTCAAAGAGGGGATTGGTCCGCAGTTGCC  >  NC_000913/934542‑935004                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                aacccACTGTTGCTCCCCAGTCTACAGACCAGATTGAGCAAACGGATCAGCAGCCAGCCGCTCAGGAGCGGTTGTAC‑CAATAGCCGCAACCCGTTGAACAGTAGCCTGTTGTGGAGCCTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAGGCGAGCCCGTGAACGTGAACAACTTGTGGCTTGGTATCAACCGATTCCAAAAACGGTTAAagaa                                                                                                                                                                                                                      <  2:195148/248‑1 (MQ=255) aaTCCACTTTTGCTCCACAGTCTACATACCAGACTGAGCAAACTTATCAGCAGCCAGCCGCTCAGGAGCCGTTGTAC‑CAACAGCCGCAACCCGTTGAACAGCAGCCTGTTGTGGAGCCTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAagaa                                                                                                                                                                                                                      <  1:395688/251‑1 (MQ=255)       cttttaatgcacaGTCTAAATACAAGACTGTAAAAACTTATCAGAAGCCAGCCGCTAAGGAGCCGTTGTACACAA‑AGCCGCAACCAGAAGAAAAGCAGCCAGTAGTGGAGCCTGAACCAGTTGTAGCAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGATGACACTGACGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCTGACACGGTTAAagaaccagt                                                                                                                                                                                                                 >  2:265526/10‑249 (MQ=255)           tGCTCCACAGTCTACATACCAGACTGAGCAAACTTATCAGCAGCCAGCCGCTCAGGAGCCGTTGTAC‑CAACAGCCGCAACCCGTTGAACAGCAGCCTGTTGTGGAGCCTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAACGAACCAGCACCg                                                                                                                                                                                                             >  1:308859/1‑250 (MQ=255)              tCCACAGTCTACATACCAGACTGAGCAAACTTATCAGCAGCCAGCCGCTCAGGAGCCGTTGTAC‑CAACAGCCGCAACCCGTTGAACAGCAGCCTGTTGTGGAGCCTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATc                                                                                                                                                                                                          >  1:382032/1‑250 (MQ=255)                         cATACCAGACTGAGCAAACTTATCAGCAGCCAGCCGCTCAGGAGCCGTTGTAC‑CAACAGCCGCAACCCGTTGAACAGCAGCCTGTTGTGGAGCCTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCACGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTg                                                                                                                                                                                              >  2:126685/1‑251 (MQ=255)                               aGACTGAGCAAACTTATCAGCAGCCAGCCGCTCAGGAGCCGTTGTAC‑CAACAGCCGCAACCCGTTGAACAGCAGCCTGTTGTGGAGCCTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCa                                                                                                                                                                                        <  1:358536/251‑1 (MQ=255)                                    gaagaaaCTTATCAGCAGCACGCCGCTCAGGATCCGTTGTAC‑CAACAGCCGCAACCCGTTGAACAGCAGCCTGTTGTGGAGCCTGACCCCGTTGTAGAAGATACAAAACCCGCGCGTCCGCCGCTTTACTTCTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGAGCAAATCTTCGCTGAAAGCAccg                                                                                                                                                                                     <  2:158188/245‑2 (MQ=255)                                         aaCTTATCAGCAGCCAGCCGCTCAGGAGCCGTTGTAC‑CAACAGCCGCAACCCGTTGAACAGCAGCCGGTTGTGGAGCCTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTg                                                                                                                                                                              <  1:151997/251‑1 (MQ=255)                                           cTTATCAGCAGCCAGCCGCTCAGGAGCCGTTGTAC‑CAACAACCGCAACCCGTTGCACAGCAGCCTGTTGTGGAGCCTGAACCCGCTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGCTTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAACGCACCTTCTGtttcc                                                                                                                                                                            >  2:77426/1‑248 (MQ=255)                                                cagcagCCAGCCGCTCAGGAGCCGTTGTAC‑CAACAGCCGCAACCCGTTGAACAGCAGCCTGTTGTGGAGCCTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt                                                                                                                                                                       >  1:331513/1‑251 (MQ=255)                                                 agcagcCAGCCGCTCAGGAGCCGTTGTAC‑CAACAGCCGCAACCCGTTGAACAGCAGCCTGTTGTGGAGCCTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTa                                                                                                                                                                      <  2:390549/251‑1 (MQ=255)                                                                  gAGCCGTTGTAC‑TAACAGCAGCAACCCGTTGAACAGCAGCCTGTTGTGGAGCCTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGACGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCATGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAcgccgc                                                                                                                                                     >  2:214301/1‑251 (MQ=255)                                                                                         aaCCCGTTGAACAGCAGCCTGTTGTGGAGCCTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCTGGc                                                                                                                                >  1:115179/1‑250 (MQ=255)                                                                                              gTTGAACAGCAGCCTGTTGTGGAGCCTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCTGGCACCTgg                                                                                                                          >  2:130333/1‑251 (MQ=255)                                                                                                tGAACAGCAGCCTGTTGTGGAGCCTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGACCGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCTGGCATCTGGCg                                                                                                                        >  1:202050/1‑251 (MQ=255)                                                                                                      gcagcCTGTTGTGGAGCCTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCTGGCATCTGGCGTGaaa                                                                                                                   >  2:224397/1‑250 (MQ=255)                                                                                                             gttgtGGAGCCTGAACCCGTGGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCTGGCATCTGGCGTGAAAAAAGCGac                                                                                                           <  1:224397/251‑1 (MQ=255)                                                                                                               tgtgGAGCCTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATACCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCTGGCATCTGGCGTGAAAAAAGCGacac                                                                                                         >  1:236988/1‑251 (MQ=255)                                                                                                                  ggAGCCTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCTGGCATCTGGCGTGAAAAAAGCGACACTgg                                                                                                      <  1:165703/251‑1 (MQ=255)                                                                                                                       cTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCTGGCATCTGGCGTGAAAAAAGCGACACTGGCGACg                                                                                                 >  1:345111/1‑251 (MQ=255)                                                                                                                                    agaagaGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGACCGTGAACCACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGCTCAAATCTTCGCTGAACGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCt                                                                                                                                   >  1:55954/1‑204 (MQ=255)                                                                                                                                                  ccGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCTGGCATCTGGCGTGAAAAAAGCGACACTGGCGACGGGGGCTGCCGCAACCGTTGCCGCGcc                                                                       >  2:161935/1‑250 (MQ=255)                                                                                                                                                                     ctactTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAAAGTGAACCACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCTGGCATCTGGCGTGAAAAAAGCGACACTGGCGACGGGGGCTGCCGCAACCGTTGCCGCGCCAGTCTTCAGTCTGGCAAAt                                                    >  1:252688/1‑250 (MQ=255)                                                                                                                                                                            gaagaaGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCTGGCATCTGGCGTGAAAAAAGCGACACTGGCGACGGGGGCTGCCGCAACCGTTGCCGCGCCAGTCTTCAGTCTGGCAAATAGCGGTgg                                            >  1:98269/1‑251 (MQ=255)                                                                                                                                                                                 agTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGCACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTACAGTAGAAGCCGCTGCCGCTGTTTACCCGCTGGCATCTGGCGTGAAAAAAGCGACACTGGCGACGGGGGCTGCCGCAACCGTTGCCGCGCCAGTCTTCAGTCTGGCCAATAGCGGTGGACcgc                                       >  1:162026/1‑251 (MQ=255)                                                                                                                                                                                     gAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATGCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCCCCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCTGGCATCTGGCGTGAAAAAAGCGACACTGGCGACGGGGGCTGCCGCAACCGTTGCCGCGCCAGTCTTCAGTCTGGCAAATAGCGGTGGACCGCGTcc                                   <  2:331513/251‑1 (MQ=255)                                                                                                                                                                                                 gCCCGTGACCGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCTGGCATCTGGCGTGAAAAAAGCGACACTGGCGACGGGGGCTGCCGCAACCGTTGCCGCGCCAGTCTTCAGTCTGGCAAATAGCGGTGGACCGCGTCCTCAGGTCaa                          >  1:107416/1‑248 (MQ=255)                                                                                                                                                                                                             gaacAACTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCTGGCATCTGGCGTGAAAAAAGCGACACTGGCGACGGGGGCTGCCGCAACCGTTGCCGCGCCAGTCTTCAGTCTGGCAAATAGCGGTGGACCGCGTCCTCAGGTCAAAGAGGGGATTGGTcc           >  2:313575/1‑251 (MQ=255)                                                                                                                                                                                                                  acTTGCGGCCTGGTATCAACCGATTCCAGAAACGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCTGGCATCTGGCGTGAAAAAAGCGACACTGGCGACGGGGGCTGCCGCAACCGTTGCCGCGCCAGTCTTCAGTCTGGCAAATAGCGGTGGACCGCGTCCTCAGGTCAAAGAGGGGATTGGTCCGCAGt      >  1:114324/1‑251 (MQ=255)                                                                                                                                                                                                                       cGGCCTGGTATCAAACGATTCCAGAAACGGTTACAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCTGGCATCTGGCGTGAAAAAAGCGACACTGGCGACGGGGGCTGCCGCAACCGTTGCCGCGCCAGTCTTCAGTCTGGCAAATAGCGGTGGACCGCGTCCTCAGGTCAAAGAGGGGATTGGTCCGCAGTTGcc  >  1:328780/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                AATCCACTTTTGCTCCACAGTCTACATACCAGACTGAGCAAACTTATCAGCAGCCAGCCGCTCAGGAGCCGTTGTAC‑CAACAGCCGCAACCCGTTGAACAGCAGCCTGTTGTGGAGCCTGAACCCGTTGTAGAAGAGACAAAACCCGCGCGTCCGCCGCTTTACTACTTTGAAGAAGTTGAAGAGAAGCGAGCCCGTGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGTACCTCCAGTAGAAGCCGCTGCCGCTGTTTCCCCGCTGGCATCTGGCGTGAAAAAAGCGACACTGGCGACGGGGGCTGCCGCAACCGTTGCCGCGCCAGTCTTCAGTCTGGCAAATAGCGGTGGACCGCGTCCTCAGGTCAAAGAGGGGATTGGTCCGCAGTTGCC  >  NC_000913/934542‑935004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑