Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	854,905	C→T	V280V (GTG→GTA)	ldtB ←	L,D‑transpeptidase LdtB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	854,905	0	C	T	100.0%	87.0 / NA	27	V280V (GTG→GTA)	ldtB	L,D‑transpeptidase LdtB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/8);  total (19/8)

CATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGTGGTAGACAGCGGGTTATGGACTTCAATATAACGGCTGCCGTCTGGCTCGGTGGTCGCTTTTACCGGCTCATCAATAAACTGTACGCGGGTACCGACCGGTACTTTCTCGAACAGGAATTTGATGTCTTCGTTACGCAGACGCACACAACCATGACTTACACGCAGGCCGATACCGAAGTTG  >  NC_000913/854688‑855151                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       cATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAACGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGc                                                                                                                                                                                                                       >  1:372707/1‑251 (MQ=255)                 cGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTTTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGAATATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAATAATTCAGACCAACCGGCATCCCGGGGCGGGTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCtt                                                                                                                                                                                                       <  2:6770/251‑1 (MQ=255)                   aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCa                                                                                                                                                                                                     <  2:161181/251‑1 (MQ=255)                                    gttagtggggttggTTTTGTTAGTTATACTCACGGACAACTAAGGTCAGGCAAATAAAAAATACGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATACCGGAGCGGTTTTTAATCGCATCATCAAGAACAACCTGGTCAACATCTGGCTGACCAGTTACTGTCTGCACGCTCTTCGTCAAGGTAATTGACACAATTTCCTGTACTTAAAACTGCGCTTCgttggt                                                                                                                                                                                    >  2:35565/14‑251 (MQ=255)                                          gggATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGTGGTAGACAg                                                                                                                                                                              <  1:377102/251‑1 (MQ=255)                                                                             aggttcgggCAAATAAAAAAGGCTGATTATGAGTCCGCCTTTTGCTTTGAGGTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCGGACCGGTTACTGTCTTCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGTGGTAGACAGCGGGTTATGGACTTCAATATAACGGCTGCGGTCTg                                                                                                                                           <  2:301176/244‑1 (MQ=255)                                                                                      cAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGTGGTAGACAGCGGGTTATGGACTTCAATATAACGGCTGCCGTCTGGCTCggtgg                                                                                                                                  >  1:195392/1‑251 (MQ=255)                                                                                            aaaaaGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTCATTCAGACGAACCGGCATACCGGAGCCGTGTTTACTCGCCTCATCAAGAACGACCAGGTCAACATCAGGCCGACCGGTTACTGACTGCACGCTCTTCGTCAGGGAAATTGGCACAATTTCCTGACCTTCAACCTGGGCTTCGGTGGTCGACAGCGGGTTATGGACTTCAATATAACGGCTGACGTCTGGCTCGGTGGACGcct                                                                                                                            >  1:272735/1‑249 (MQ=255)                                                                                                 ggCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGTGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAACGGTTACTGTCTGAACGCTCTTCGTCAGGGAAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGGGGTAGACAGCGGGTTATGGCCTTCAATATAACGGCTGCCGTATGGCTCGGAGGTCGCttttccc                                                                                                                       >  2:387863/1‑248 (MQ=255)                                                                                                        tATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGTGGTAGACAGCGGGTTATGGACt                                                                                                                                                                 >  1:259578/1‑202 (MQ=255)                                                                                                        tATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGTGGTAGACAGCGGGTTATGGACt                                                                                                                                                                 <  2:259578/202‑1 (MQ=255)                                                                                                         aTGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTc                                                                                                                                                                                                      <  2:67850/164‑1 (MQ=255)                                                                                                         aTGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTc                                                                                                                                                                                                      >  1:67850/1‑164 (MQ=255)                                                                                                                         cTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGTGGTAGACAGCGGGTTATGGACTTCAATATAACGGCTGCCGTCTGGCTCGGTGGTCGCTTTTACCGGCTCATCAATAAACTGTACGCgg                                                                                               >  2:334051/1‑251 (MQ=255)                                                                                                                                 tttGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGTGGTAGACAGCGGGTTATGGACTTCAATATAACGGCTGCCGTCTGGCTCGGTGGTCGCTTTTACCGGCTCATCAATAAACTGTACGCGGGTaccgac                                                                                       >  1:361074/1‑251 (MQ=255)                                                                                                                                      aGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGTGGTAGACAGCGGGTTATGGACTTCAATATAACGGCTGCCGTCTGGCTCGGTGGTCGCTTTTACCGGCTCATCAATAAACTGTACGCGGGTACCGACCGGt                                                                                   >  1:392832/1‑250 (MQ=255)                                                                                                                                       gATTAATTACGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTTAATGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGTGGTAGACAGCGGGTTAAGGCCTTCAATATAACGGCTGCCGTCTGGCTCGGTGGTCGCTTTTACCGGCTCATCAATAAACTGAAGGCGGGTACCGACCGGTAc                                                                                 >  2:285630/1‑251 (MQ=255)                                                                                                                                            aTTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACATGGTCAACATCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACGGGGCTTCGGTGGTAGACAGCGGGTTATGGACTTCAATATAACGGCTGCCGTCTGGCTCGGTGGTCGCTTTTACCGGCTCATCAATAAACTGTACGCGGGTACCGACCGGTACTTtct                                                                            <  1:95345/251‑1 (MQ=255)                                                                                                                                             ttCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGATGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGTGGTAGACAGCGGGTTATGGACTTCAATATAACGGATGCCGTCTGGCTCGGTGGTCGCTTTTAACGGCTCATCAACAAACTGTACGCGGGTACCGACCGGTACTTtctc                                                                           >  1:34437/1‑251 (MQ=255)                                                                                                                                                    gAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGTGGTAGACAGCGGGTTATGGACTTCAATATAACGGCTGCCGTCTGGCTCGGTGGTCGCTTTTACCGGCTCATCAATAAAATGTACGCGGGTACCGACCGGTACTATATCGAACAgg                                                                    >  2:365298/1‑251 (MQ=255)                                                                                                                                                                 ggAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGTGGTAGACAGCGGGTTATGGACTTCAATATAACGGCTGCCGTCTGGCTCGGTGGTCGCTTTTACCGGCTCATCAATAAACTGTACGCGGGTACCGACCGGTACTTTCTCGAACAGGAATTTGATGTCtt                                                       >  1:315377/1‑251 (MQ=255)                                                                                                                                                                       gTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGTGGTAGACAGCGGGTTATGGACTTCAATATAACGGCTGCCGTCTGGCTCGGTGGTCGCTTTTACCGGCTCATCAATAAACTGTACGCGGGTACCGACCGGTACTTTCTCGAACAGGAATTTGATGTCTTCGTTAc                                                 >  1:403562/1‑251 (MQ=255)                                                                                                                                                                                              caacCTGGTCAACATCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGTGGTAGACAGCGGGTTATGGACTTCAATATAACGGCTGCCGTCTGGCTCGGTGGTCGCTTTTACCGGCTCATCAATAAACTGTACGCGGGTACCGACCGGTACTTTCTCGAACAGGAATTTGATGTCTTCGTTACGCAGACGCACACAACCATGACtt                          >  1:393214/1‑251 (MQ=255)                                                                                                                                                                                                         aCATCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGTGGTAGACAGCGGGTTATGGACTTCAATATAACGGCTGCCGTCTGGCTCGGTGGTCGCTTTTACCGGCTCATCAATAAACTGTACGCGGGTACCGACCGGTACTTTCTCGAACAGGAATTTGATGTCTTCGTTACGCAGACGCACACAACCATGACTTACACGCAGGcc               <  1:327047/251‑1 (MQ=255)                                                                                                                                                                                                           ctCTGGCTGACCGGTTACTGTCTGCACGCTCTTCGTCATGGTCATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGTTGGTGGACGGCGGGTTATGGACTTCAATATAACGGCTGCCGTCTGTCTCGAAGGTCGCTTTTACCGGCTCATCAGTAAACTGTACTCGGGTCCCGACCGGTACTTTCTCGAACAGGAATTTGATGTCTTCGTTAAGCAGACGCCCACAACCATGACTTaaacacatgccgc             >  2:313956/2‑239 (MQ=255)                                                                                                                                                                                                                  tGACCGGTTACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGTGGTAGACAGCGGGTTATGGACTTCAATATAACGGCTGCCGTCTGGCTCGGTGGTCGCTTTTACCGGCTCATCAATAAACTGTACGCGGGTACCGACCGGTACTTTCTCGAACAGGAATTTGATGTCTTCGTTACGCAGACGCACACAACCATGACTTACACGCAGGCCGATACCGa       >  2:160314/1‑250 (MQ=255)                                                                                                                                                                                                                      cGGTTACTGTCTGCACGTCCTTCGTCCGGGCAATCGGCACAATTTCCTGACCTTCAAACCGGGCTTAGGCGGTCGACAGCGGGTTATGGACTTCAATCTCACGGCTGCCGTCTGGCTCGGTGGTCGCTTTTACCGGCTCATCAATAACCTGTACGCGGGCACCGACCGGTACTTTCTCGAACAGGCATTTGATGTCTTCGTTACGCAGACGCACCCAACCATGACTTTCACGCAAGCCGATACCGAAGTTg  >  2:174212/1‑251 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       CATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACCGGTCACTGTCTGCACGCTCTTCGTCAGGGTAATTGGCACAATTTCCTGACCTTCAAACTGGGCTTCGGTGGTAGACAGCGGGTTATGGACTTCAATATAACGGCTGCCGTCTGGCTCGGTGGTCGCTTTTACCGGCTCATCAATAAACTGTACGCGGGTACCGACCGGTACTTTCTCGAACAGGAATTTGATGTCTTCGTTACGCAGACGCACACAACCATGACTTACACGCAGGCCGATACCGAAGTTG  >  NC_000913/854688‑855151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑