Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,240	G→A	L21L (TTG→TTA)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,240	0	G	A	100.0%	155.3 / NA	45	L21L (TTG→TTA)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/24);  total (21/24)

AGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGA  >  NC_000913/4637998‑4638479                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                agcGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGAt                                                                                                                                                                                                                                         <  1:92280/251‑1 (MQ=255)  gcGGTCTGGGGTGGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACGATGTTGAAATCCCCCCTGGTCTGGAAAATGACTAGTCTGTTTGGTTCAGTATTGCTGTTGTTAATCCCGATa                                                                                                                                                                                                                                        <  2:392868/251‑1 (MQ=255)         ggggTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTg                                                                                                                                                                                                                                  <  1:9588/250‑1 (MQ=255)         ggggGTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGc                                                                                                                                                                                                                                    <  2:143416/248‑1 (MQ=255)          gggTTGGCGTTCGGCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGAt                                                                                                                                                                                                                                <  2:162094/251‑1 (MQ=255)            gTTGGCGTTCGGCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGGCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTATCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTc                                                                                                                                                                                                                              <  1:252344/251‑1 (MQ=255)                        cAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGAtt                                                                                                                                                                                                                   <  1:91740/250‑1 (MQ=255)                         aGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGt                                                                                                                                                                                                                 >  1:127326/1‑251 (MQ=255)                                    ccGTTTGTTTAACGGCGACGCCACCCTGCGCACCGTGCCCGAAGGTGGAGTGCTGGCCTAGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGACTTCGTATACTGCTACCATTTCAAACGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGTTAATGCTGATTCGGCCGGGGATTGTCGAACTTGCTg                                                                                                                                                                                                      >  2:263177/1‑251 (MQ=255)                                         tgtttAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGCTTAc                                                                                                                                                                                                 >  1:79964/1‑251 (MQ=255)                                          gtttAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCAAATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCAATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGAGCAGTATTGCTGTTGTTAATTCCGATAATGCAGATTAGGCAGGTGATTGTCGAACGTGCTGATTAcc                                                                                                                                                                                                >  1:403838/1‑251 (MQ=255)                                            ttAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCg                                                                                                                                                                                               <  1:90969/250‑1 (MQ=255)                                                cGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCg                                                                                                                                                                                          >  1:271288/1‑251 (MQ=255)                                                cGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCg                                                                                                                                                                                          <  1:1538/251‑1 (MQ=255)                                                     aaGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCGTCGACTTCACCGTCTCTTCACATATCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGATAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTTCTGTTGTTAAGTCCTAGATTGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTTGCGAtgtg                                                                                                                                                                                     <  2:392230/251‑1 (MQ=255)                                                               gcgcAAGGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCCACTTCACCGTCACTTCACATAGCTTAAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGTACGTGCTGCTTACCGTAGCGCTGTGGAAGATGCg                                                                                                                                                                            >  2:387577/1‑250 (MQ=255)                                                                   aaCGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCg                                                                                                                                                                       <  1:106402/251‑1 (MQ=255)                                                                               ggTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTGGCGATGTGGAAGATGAGATTCGCCAAAGTAcc                                                                                                                                                             >  2:117352/1‑249 (MQ=255)                                                                                  ggCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCgg                                                                                                                                                        <  2:315459/251‑1 (MQ=255)                                                                                     gTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGcc                                                                                                                                                     <  1:370799/251‑1 (MQ=255)                                                                                                          cACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCCAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCt                                                                                                                                >  1:259797/1‑251 (MQ=255)                                                                                                                       caTAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCt                                                                                                                   >  2:104544/1‑251 (MQ=255)                                                                                                                          aGCTTCAAATTCTTCCCACATAGTCCTCGTATCCTTCTGCCATTTCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTTGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATTCGATTCGCCAAAGTACCATCGGGCCGCAAAAACTCGTTGGTCCGCTCATCGCTATTCCtgtg                                                                                                                <  2:273800/251‑1 (MQ=255)                                                                                                                                    tcttcCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGCGCttt                                                                                                      >  2:117730/1‑251 (MQ=255)                                                                                                                                            caTAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTg                                                                                              >  1:122733/1‑251 (MQ=255)                                                                                                                                                     cGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGCAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAag                                                                                       >  2:190839/1‑249 (MQ=255)                                                                                                                                                     cGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTTGTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGGTTGCGGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACGCGTGGGGCCGCTCAGCGCTATTCCTGTTACCGAGCTTTATACGGTGCAGTAagag                                                                                     <  2:204993/251‑1 (MQ=255)                                                                                                                                                                   aTTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTgg                                                                        >  1:49526/1‑250 (MQ=255)                                                                                                                                                                        aaaGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCgg                                                                   >  1:304789/1‑250 (MQ=255)                                                                                                                                                                         aaGGAGACGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTGGGTGCATTATTGCTGTTGTTAATTCCGATAATGCTGATGCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGGTGGGCCGCTCATCGCTATTCCTGTGCCCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGaa                                                                 <  2:77763/251‑1 (MQ=255)                                                                                                                                                                         aaGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGCTTCACGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCCGGAAGAGGATAAAACAGTGGAGCGGa                                                                  >  1:98/1‑250 (MQ=255)                                                                                                                                                                         aaGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGa                                                                  >  1:99009/1‑250 (MQ=255)                                                                                                                                                                         aaGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCAGCAACAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGCGCTTTATACGGTGCCGGAAGAGGATACACCCGTGGAGCGGa                                                                  >  2:231952/1‑250 (MQ=255)                                                                                                                                                                           ggAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAAc                                                               <  1:83564/251‑1 (MQ=255)                                                                                                                                                                              gaagaCTATGGTGAAATCCCCCCTGTTCTGGAAAATGTCTAGCCTGTTGGGTGCTGTATTGCTGTTGTTAAGTCCGAGAATGCTGATTCGGCAGGTGAATGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACGCGTTGGGCCGCTCATCGCTATTCCTGTGAGCGAGCTTTATACAGTGCAGGAAGAGGATAAAACAGTGGAGCGGAAACGaa                                                            <  2:74061/251‑1 (MQ=255)                                                                                                                                                                                 gaCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATTCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGATCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGtt                                                         <  2:274522/251‑1 (MQ=255)                                                                                                                                                                                                  cccTGTTCTGGACAATGACGAGCCTGTTTAGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGGAGGTGATTGTCGAACGTGATGATTACCGTAGCGATGTGGAAGATGCGATTCGCAAAAGTCACAGCGTGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACAGAGCTTTATACGGTGCAGGAAGAGGATAAAAACGTGGAGCGGAAACGCAGTTTTATCCATTTTTGGtt                                         >  2:243000/1‑250 (MQ=255)                                                                                                                                                                                                           ggAAAATGAATCGCATGTTAGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTAGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGCGCTTTATACGGTGCCGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTc                                >  2:66833/1‑250 (MQ=255)                                                                                                                                                                                                           ggAAAAGGACTAGCCGGTTTGGTGCAGTATTGCTGGTGTTAATTCCGATAATGCTGAGTCGGCAGGTGATGGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACACGTTGGGGCGCTCATCGCTAGTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTGAATCCATTTGTGGTTACCTGAGTCa                               <  2:413936/251‑1 (MQ=255)                                                                                                                                                                                                              aaaTGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGTTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTCTATCCATTTTTGGTTTCCTGAGTCAttg                            <  2:403838/251‑1 (MQ=255)                                                                                                                                                                                                                aTGATTAGCCTGTTTGGTGCAGGATTGCTGTTTGTAATGCCGATAATGTTGATTCGGCAGGTTAGTGTCGAACGTGCTGATTACCGTTGCGAGGTGGAAGATGCGAGTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGTCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCAGAAACGAAGTTTTATCCATTTTTGTTTACCTTCGTCAttgag                          <  2:295103/251‑2 (MQ=255)                                                                                                                                                                                                                   aCTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGGGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCAttgatggt                       <  1:167399/251‑1 (MQ=255)                                                                                                                                                                                                                      aGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCAttgatggttg                     <  1:117730/250‑1 (MQ=255)                                                                                                                                                                                                                                       aTTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTAATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCTGGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTAATTGATGGTTGATGGCAATCAGAACgagg   >  2:1372/1‑248 (MQ=255)                                                                                                                                                                                                                                           cTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCGGAACGTGga  >  1:246946/1‑248 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                AGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGA  >  NC_000913/4637998‑4638479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑