Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,925	G→C	R77P (CGC→CCC)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,925	0	G	C	100.0%	172.7 / NA	55	R77P (CGC→CCC)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (23/32);  total (23/32)

GCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGC  >  NC_000913/4636680‑4637166                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  gCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGt                                                                                                                                                                                                                                              <  1:303567/251‑1 (MQ=255)  ccTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGtt                                                                                                                                                                                                                                             >  1:148289/1‑251 (MQ=255)              gTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACAGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGATGGCGCGTCCCGAGTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAatat                                                                                                                                                                                                                                 >  1:213371/1‑251 (MQ=255)               tAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATc                                                                                                                                                                                                                                >  1:287424/1‑251 (MQ=255)                aaTGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATc                                                                                                                                                                                                                                <  1:207143/250‑1 (MQ=255)                aaTGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATc                                                                                                                                                                                                                                <  1:314073/250‑1 (MQ=255)                    cGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTgg                                                                                                                                                                                                                           <  1:107017/251‑1 (MQ=255)                     gTATCGGCAGTCTGTTGTTGCTGGGCTATTTTTTCCTGGTTGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGGGCGAAGAGCAACGGAGGGGACGTTGATCGACCCCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGTACCCAACGCATGGGCAACTGGCGCAGGCGTTTAAGCAGCGACAACATCCCCCGTTTCGCGCCAATATCGGTGGc                                                                                                                                                                                                                          <  2:266338/251‑1 (MQ=255)                                        gCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCGGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCGGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAATTGCGCAAt                                                                                                                                                                                                        <  1:137511/250‑1 (MQ=255)                                                attgtttacgggtgtgggtggcAGCGTGGTTCGGACTGGCCATTGTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGTCGTTTAATCAGCTACAACATCCCCCGTTTTGCGCCAATATCGGTGGCATTAGCAAGGTGCGCAATTAATATCAt                                                                                                                                                                                               <  2:294563/235‑1 (MQ=255)                                                aTTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGGGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTTGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATc                                                                                                                                                                                                 <  2:26998/249‑1 (MQ=255)                                                aTTTTTTACTGGAGGCGGTGGCAGACTGGTTCGTACCGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGAGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCACCTGGGGCAGGCGTTTTATCAGCTACAACATCCCCCGTTTCCCGCCAATATCGGTGGCATTAACAAAGTGCGCCACGATTATCAt                                                                                                                                                                                               >  2:303304/1‑251 (MQ=255)                                                aTTTTTGACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTGAAACCGGGCGTGCGAAGATCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCGCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCTTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCTGTGGCATTAACAAAGTGCGCAATGAATATc                                                                                                                                                                                                 <  1:383231/249‑1 (MQ=255)                                                       aCTGGTGGCGGTGGCAGTCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGAACGACACCGCAACGTTGCGGGCGGAGCTGGCGCGGCCCGATTTGCTCTCGGGGGACCCAACGCATGGGCAACTGTCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTGCGCGTCAATATCGGTGGCATTAACAAAGAGCGCAATTAACATCAAGTCtag                                                                                                                                                                                         <  2:265434/250‑2 (MQ=255)                                                                          tGGTTCGTACTGGACATTTTTGTCAACGAAGTTACACCGGGCGTGCGAAGCGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAAAATCCACCGTTTCGCGCCAATATCGGTGGCCTTAACAAAGTGCGCAATGAATATCATGTCTATCTGACCGATGCGCAgggaa                                                                                                                                                                      >  2:273272/1‑248 (MQ=255)                                                                                gTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTAtt                                                                                                                                                               <  1:257114/251‑1 (MQ=255)                                                                                     ggCCATTTTTGTCAAAGATGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGtt                                                                                                                                                            <  1:262996/249‑1 (MQ=255)                                                                                               ggcAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCaaa                                                                                                                                                <  2:327403/249‑1 (MQ=255)                                                                                                   aagaagTTAAACCGGGCGTGCGAAGAGCAAGGGAGGGGACGTTGATCGACACCGTAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCGGGGGACCCAACGCATGTGCATCTGGCGCAGGCGTTTAATCAGCTACAACATCCCTCGTTTCGCGCCAATATCGGTGGCATTAATAAAGTGCGCAATGAATATCGTGGCTATATGACCGATGCGCAGGGTAAAGTATTGTTCGATTCTGCATATaaa                                                                                                                                            <  2:211482/251‑1 (MQ=255)                                                                                                    agaagTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAg                                                                                                                                           <  2:70799/251‑1 (MQ=255)                                                                                                            aaCCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTgg                                                                                                                                    >  1:52245/1‑250 (MQ=255)                                                                                                               cGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTggcc                                                                                                                                  >  1:148127/1‑247 (MQ=255)                                                                                                                gggCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAgg                                                                                                                               >  1:152933/1‑251 (MQ=255)                                                                                                                             gCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATAGGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTg                                                                                                                  <  2:117905/251‑1 (MQ=255)                                                                                                                              cAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCGGGCGCGTCCCGATTTGCTCTCGGGGGACCAAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATAACGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTgg                                                                                                                 >  1:180716/1‑251 (MQ=255)                                                                                                                               aaCGGAGGGGACGTTGATCGCCACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTgg                                                                                                                 >  1:299269/1‑250 (MQ=255)                                                                                                                                    gggggACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGAGTATTCGCGCTGGAATGAc                                                                                                           <  1:112987/250‑1 (MQ=255)                                                                                                                                      gggACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGCCCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAAGAAAGCCGTTGTACAGGATTATTCGCGATGGAATGACGt                                                                                                         <  2:87626/251‑1 (MQ=255)                                                                                                                                          cGTTGATCGACACCGCAACGTTGCTGGCGGAGCGGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTgg                                                                                                     <  2:135423/251‑1 (MQ=255)                                                                                                                                          cGTTGATCGACACCGCAACGTTCCTGGCGGAGCTCGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGGGGCATTAACCAAGTGCGCCATGAATATCATGTCTATATAACCGATGCGCAGGGAACAGTATTGTTCGTTTCGGCAAATAAAGCCGGTGGACAGGATTATTCGCGCTGGAATGACGTcagg                                                                                                     >  2:82636/1‑248 (MQ=255)                                                                                                                                               aTCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCTATTTGCTCTCGGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACAGCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAAGATCAGGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAAc                                                                                                <  2:236637/251‑1 (MQ=255)                                                                                                                                                 cGACACCGCAACGGTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGt                                                                                              <  1:60604/251‑1 (MQ=255)                                                                                                                                                  gACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGtt                                                                                             <  2:148289/251‑1 (MQ=255)                                                                                                                                                          aaCGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTc                                                                                     <  1:398863/251‑1 (MQ=255)                                                                                                                                                            cGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAg                                                                                   <  2:125036/251‑1 (MQ=255)                                                                                                                                                              ttGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGGGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCACAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGt                                                                                  >  2:1619/1‑250 (MQ=255)                                                                                                                                                                            ggCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCCGTATGGTGCGCgcagc                                                                    >  2:301663/1‑250 (MQ=255)                                                                                                                                                                                           ggattcgggggACCCAACGCATGGGCAACGGGCGCAGGCGTTTAATCAGCTAAAACATCCCCCGTTTCGCGCCAAGATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGTCGTTGGAAAGGATTATGCGCGCTGGAATGACGGCTGGCTAACGTTGCGTGGGCAGTATGGTGCGCGCAGCACGTGGCAAAATCCt                                                     <  1:286402/243‑1 (MQ=255)                                                                                                                                                                                                      aCCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGa                                          >  1:90572/1‑250 (MQ=255)                                                                                                                                                                                                       cccAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGaaa                                        <  1:303304/251‑1 (MQ=255)                                                                                                                                                                                                       cccAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGAAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGaa                                         >  1:30897/1‑250 (MQ=255)                                                                                                                                                                                                        ccAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGaa                                         >  1:248889/1‑249 (MQ=255)                                                                                                                                                                                                            cGCATGGGCAACTGGAGCAGGCGTTTAATCAGCTAAAACCTCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGAGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGAATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTAGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGACAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGCTCCCGAACGTTCt                                   >  2:277347/1‑251 (MQ=255)                                                                                                                                                                                                               aTGGGCTACTGGCGCAGGCGTTTACTCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGACTATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAGAGTTCtgtg                                >  2:215691/1‑251 (MQ=255)                                                                                                                                                                                                                  cgCAACTGGCGCAAGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCCTGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGatg                             >  2:58900/2‑251 (MQ=255)                                                                                                                                                                                                                         ggCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAAGATCGGTTGCATTAACAAAGTGCGCAATGAAGATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGAGTATTCGCGCTGTAATGACGTGTGGCTAACGTGTCGTGGTCAGGATGGTGCTCGCGGGACGTTGCAAATTCCTGCCGATCCCGAAAGTTCTGTGAGGTATGtt                       <  2:370803/250‑1 (MQ=255)                                                                                                                                                                                                                         ggCGCAGGCGTTTAATCAGCTACAACATCCCCCGTGTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAAGATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTTTTCGATTCGGCAAATAAAGCCGTTGGACAGGATGATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTGCTGTGATGTATGtt                       <  2:383444/250‑1 (MQ=255)                                                                                                                                                                                                                           cgcAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTGGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGcc                    <  2:287424/251‑1 (MQ=255)                                                                                                                                                                                                                                      aaTCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTa          <  1:389718/250‑1 (MQ=255)                                                                                                                                                                                                                                      aaTCAGCTACAACAGCCCCCGTTTCGCGCCAATATTGGTGGCATGAACAAAGTGCGCAAGGAATATTATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTTGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCTCCGATTAt         <  1:254843/251‑1 (MQ=255)                                                                                                                                                                                                                                       aTCAGCTACACCATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTCCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCACAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAACATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATg        >  2:21109/1‑251 (MQ=255)                                                                                                                                                                                                                                          aGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCCCGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGAc     >  1:402804/1‑251 (MQ=255)                                                                                                                                                                                                                                          aGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGAc     >  1:34294/1‑251 (MQ=255)                                                                                                                                                                                                                                          aGCTACAACATCCCACGTTGCGCGCCAATATAGGTGGCAGTAACAAAGTGCGCAATTAAGATGATGGCTAAATGACCGATGCGCAGGGCAAAGTATTGTTCGAGTCGGCAAATAAAGCCGTTGGACAGGATTATTAGCGCTGGAATGACGTTTGGCTAACGTTGCGTGGTCAGTATGGTTCGCGCAGCACGTGGCAAAAGCCTGCCGATCCCGAAAGTTCTGTGATGTATGGTGCCGCACCGAGTATGGAc     <  1:366630/251‑1 (MQ=255)                                                                                                                                                                                                                                             tACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAACGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGc  >  1:186483/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  GCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGC  >  NC_000913/4636680‑4637166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑