Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,321,884	C→T	R180H (CGC→CAC)	phnF ←	putative transcriptional regulator PhnF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,321,884	0	C	T	95.0%	218.4 / ‑4.0	84	R180H (CGC→CAC)	phnF	putative transcriptional regulator PhnF
Reads supporting (aligned to +/- strand):  ref base C (3/0);  major base T (27/49);  minor base G (4/0);  total (35/49)
Fisher's exact test for biased strand distribution p-value = 1.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCT  >  NC_000913/4321646‑4322128                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       gtgCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAAt                                                                                                                                                                                                                                              <  1:92355/249‑1 (MQ=255)   gcgcCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTcc                                                                                                                                                                                                                                           <  1:304468/250‑1 (MQ=255)    cgcCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCAGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCgg                                                                                                                                                                                                                                         <  1:166697/251‑1 (MQ=255)    cgcCAGCACGGACATCCAGTGCTGGCGGGCCGCGGGATCTGCGTGCCTTAAGGGCTCCATAGGGAATTCAA‑CACTGTCGGCGCGCGATAGGCTGACGGAGTACTCCGACCGGCTGCTTTCACCGTCACGGGGGATAAGGGTGCGCACGCACCGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGCGGCTGGTTCTGGTCTGGCTGCGGCGCAGCCCGATTCCgg                                                                                                                                                                                                                                         >  1:31604/1‑251 (MQ=255)       cAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGttt                                                                                                                                                                                                                                      <  1:7561/251‑1 (MQ=255)           aCGGACATCCGTTGCGGGCGGGTCGCGGTATCTGCGTGCATTCAGAGCGCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCCCGGTGGTTAAGGGTGCGCTCGTACAGCAGCGGCGACATATTCGGGATTTCAAGTCGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTc                                                                                                                                                                                                                                  <  1:403459/251‑1 (MQ=255)              gccagccAGTGCTGGCGGGGCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCTGCAGGCGGCCGGAGTACTCCGCCGGGCTGCTTGCACCGTCACGGTGGTGAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGTCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTcgcg                                                                                                                                                                                                                               <  2:164638/246‑1 (MQ=255)                            gCGGGTCGCGGTATCTGCGTGCATTCAGTGCTTCATAGTGAATTCAGTCA‑GGGCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATAGTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGAGCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCa                                                                                                                                                                                                                <  2:31414/251‑1 (MQ=255)                            gCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCa                                                                                                                                                                                                                <  1:138110/251‑1 (MQ=255)                                 gcgcgGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAg                                                                                                                                                                                                           <  1:377174/250‑1 (MQ=255)                                     ggTATCTGCGTGCGTTCAGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGTAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGTGTCTGGCTGCGGAGCAGCGCAATTCCGGTTTGCTCGCGCCGAAAAC‑CGGGCAGCGcgccgc                                                                                                                                                                                                       >  1:95006/1‑251 (MQ=255)                                     ggTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGGGCTGAGCCGCGTCTGGCTGCGGTGCAGCGCCATTCCGGTTTGCTCGCGCAGAAAAA‑CGGGCAGCGAGCCGc                                                                                                                                                                                                       >  2:148000/1‑251 (MQ=255)                                      gTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTCAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTTGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGAAGGAAAT‑CGTGCAGCGAGCCGCt                                                                                                                                                                                                      >  1:56286/1‑251 (MQ=255)                                      gTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGGCATGCTTCGTTGCAGCGCCATTTCGGTTTGCCCGCGCAGGAAAT‑tgtggcgcgcgccgcc                                                                                                                                                                                                      >  2:157694/1‑239 (MQ=255)                                        aTCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTg                                                                                                                                                                                                     <  1:57592/250‑1 (MQ=255)                                                gCATTCAGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCg                                                                                                                                                                                             <  1:146854/250‑1 (MQ=255)                                                  aTTCAGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGGGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTg                                                                                                                                                                                          >  1:192645/1‑251 (MQ=255)                                                  aTTCAGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGATCTGCTTTCACCGTCACGGGGGTTCAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGGTTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGGGCTGATCCGCGTCGTGCTTCGGTGCCGCGCGTTTGCGGGTTTCTCGCGCCGAAAAT‑CGTGcgccgggccgccgtcctagcgttg                                                                                                                                                                                          >  2:321396/1‑228 (MQ=255)                                                   ttCAGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCCCGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGGCCTGCTGCGGGGCCGCGCCATTCCGGTTTGCTCGCGCCGCAAATACG‑GCAGCGCGCCGCTGTCGGAGCCTTGc                                                                                                                                                                                         >  2:188181/1‑251 (MQ=255)                                                      aGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGc                                                                                                                                                                                      >  1:382455/1‑251 (MQ=255)                                                      aGTGCTCACTAGTGAATTCAATCA‑TGTGGGCGCGCGTCATGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGGTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGc                                                                                                                                                                                      <  2:214681/251‑1 (MQ=255)                                                         gCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTc                                                                                                                                                                                   <  1:144347/251‑1 (MQ=255)                                                         gCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTc                                                                                                                                                                                   <  1:292164/251‑1 (MQ=255)                                                            ccATAGGGAATTCAATCA‑GGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCGCGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGc                                                                                                                                                                                <  2:347680/251‑1 (MQ=255)                                                                   gAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAggg                                                                                                                                                                         <  1:275907/251‑1 (MQ=255)                                                                      ttCAATCA‑TGTAGGCGCGCGTCAGACTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGCGGTTAAGGGTGCGCACGCACAGCAGCTGCGACATATTCGGGCTTTCACGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGAGGCGGTGCAGAGCAATTCCGGTTTGCTCGCGCAGCACAT‑CGACCAGCGAGCCGCTGTCGAAGctcatccgcggcgggccgagggggg                                                                                                                                                                      >  2:362531/1‑227 (MQ=255)                                                                        cAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAgg                                                                                                                                                                    <  1:258642/251‑1 (MQ=255)                                                                           tCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGGTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGGGAgggcc                                                                                                                                                                 >  1:187604/1‑248 (MQ=255)                                                                            tcctGTCGGCGCGCGTCAGGCTGCCGGAGTACTCCGCCGGTCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGGCGCTGGCACTCTTTGGCCTGGGCGCGGCTAGCGCTTATCCCCGTCTGGCTGATGCACCACGCAATTCCGGTTTGCTTGCGCCGAAAGT‑CGTGCCGCGGCCCGCTGTCAGAGCGTTTCCGCGCCGGCCAGAGGGCGAAGTcc                                                                                                                                                                 >  1:236863/4‑251 (MQ=255)                                                                            cA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTATCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTcc                                                                                                                                                                 <  1:266268/250‑1 (MQ=255)                                                                                gTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATACGCGTCTGGCTGCGGGGCAGCGCAATTCCGGTTTGCGCGCGCAGAAAAT‑CGTGCAGGGAGCCGCTGTCGAAGGGTTGCCACGTCGGCCAGGGGGTGAgggtcgcgg                                                                                                                                                             >  2:13751/1‑244 (MQ=255)                                                                                             aGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGAt                                                                                                                                               <  1:393429/251‑1 (MQ=255)                                                                                                  gACGGAGGACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAG‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTTGTCGATTAAAc                                                                                                                                          <  1:321396/251‑1 (MQ=255)                                                                                                     ggAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCCCAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGGCTGGCTGCGGTGCCACGCAATTCCGGGTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGGGAGGTCCGCG‑AAGTAGTGGTCGATTAAAc                                                                                                                                          >  1:61961/1‑248 (MQ=255)                                                                                                        gTACTCCGCCGTGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAgcg                                                                                                                                    <  1:211420/251‑1 (MQ=255)                                                                                                             ccgccgGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACg                                                                                                                               <  1:303768/251‑1 (MQ=255)                                                                                                             ccgccgGGCTGCTTTCACCGTCACGGTGGTGAAGGGGGCGGACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCTGTTTGCTCGCGCGGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACg                                                                                                                               <  2:186197/251‑1 (MQ=255)                                                                                                              cgccgGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTTGCTGCGGTGCAGCGCCATTCCGGGTTTCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAACGTCGGCCCGAGGGTGAGGTCCGCA‑AAATAGTGGTCGATTTAACAGAGCGCGACGc                                                                                                                              >  1:2141/1‑251 (MQ=255)                                                                                                                  gggCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGtt                                                                                                                          <  1:351950/251‑1 (MQ=255)                                                                                                                     ctgctTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGGGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGa                                                                                                                        >  1:280019/1‑250 (MQ=255)                                                                                                                          ttCACCGTCTCGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACacga                                                                                                                   <  1:305214/250‑1 (MQ=255)                                                                                                                            cACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGc                                                                                                                <  1:94855/251‑1 (MQ=255)                                                                                                                              ccGTCACGGTGGTTAAGGGCGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTTGCCGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGGGCAGCGAGCCGCTGTCGAAGCGTTGCCGCGGCGGCCAGAGGGGGAGGGCCGCG‑CAGGAGGGGGCGGTTCAACAAAAGGCGACGCCGTTGACAcgccgc                                                                                                                >  2:168029/1‑249 (MQ=255)                                                                                                                               cGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAgg                                                                                                             <  1:102377/251‑1 (MQ=255)                                                                                                                               cGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAgg                                                                                                             <  1:248631/251‑1 (MQ=255)                                                                                                                                               ggTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGa                                                                                             <  1:45423/251‑1 (MQ=255)                                                                                                                                                            agcagcGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCCGAGGGTGAGGTCCGCG‑AAGGAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGGGGATGACGTTCTCCCCCTc                                                                                >  1:95990/1‑251 (MQ=255)                                                                                                                                                             gcagcGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGGGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGCCGCCGTTGACACGACGCAGGGTGCGCAGGGGGATGACGTTCTCCCCCTCg                                                                               >  1:162929/1‑251 (MQ=255)                                                                                                                                                              cagcGGCGACATATTCGGGATTTCAAGACGCTGGCTCTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑Aagtag                                                                                                                                                       <  2:237056/178‑1 (MQ=255)                                                                                                                                                              cagcGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGGGGGTGAGGTCCGCG‑Aagtag                                                                                                                                                       >  1:237056/1‑178 (MQ=255)                                                                                                                                                               agcGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGGGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGGGAGGTCCGCG‑AAGTAGTGGGCGCTTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCggg                                                                             >  1:300829/1‑250 (MQ=255)                                                                                                                                                                  ggcgACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGGTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGGCCGCG‑GGGTAGGGGGCGATTAAACAGAGCGCGACGCCGTTGACACGACCCAGGGGGCGCAGGGGGAGGACGTTCTCCCCCTCgggatt                                                                          >  2:116040/1‑247 (MQ=255)                                                                                                                                                                     ggcATATTCGGGATTGCAAGACGCTGGCACTCTGTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCGGCGCAAGTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGGGGTCGATTAAACATAGCGCGACGCCGGTGACACGGCGCAGGGTGCGCAGGGGGATGACGTTCGCCCCCTCGGTAATccc                                                                       <  2:24212/249‑1 (MQ=255)                                                                                                                                                                     gACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGGCTGGCTGCGGTGCCGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CCTGCAGCGGGCCGCTGTCGGAGCGTTGCAGCGTCGGCCCGAGGGGGAGGGCCGCGCAAG‑AGGGGGCGGTTAAACAGAACGCGACGCCGTTGACACGGGGCAGGGGGCGCAGGGGGGTGACGTTCTCCCCCCCGGTAATccc                                                                       >  2:105621/1‑251 (MQ=255)                                                                                                                                                                       cATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCa                                                                     <  1:177578/251‑1 (MQ=255)                                                                                                                                                                          atTCGGGATGTTAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTg                                                                  <  2:65837/251‑1 (MQ=255)                                                                                                                                                                               ggATTTCAAGACGCGGGCACTCTTGGGCCTGGGCGCGGCGGGCGCTGAACCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGAGGGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCa                                                             <  2:3123/251‑1 (MQ=255)                                                                                                                                                                                   tgcAAGGCGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCGGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCg                                                          <  2:257283/248‑1 (MQ=255)                                                                                                                                                                                     caacaCGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGTGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGATGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAAGCCCCAGTGCGTCAGCGAc                                                        <  2:201266/246‑1 (MQ=255)                                                                                                                                                                                      aaGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCGCGTTAATCCCCAGTGCGTCAGCGACGt                                                      <  2:366627/251‑1 (MQ=255)                                                                                                                                                                                       aGACGCTGGCGCTCTTTGGTCTGGGCGAGTCGGGCGCGGATCCGCGTCTGGCTGCGGTGTAGCGCAAGTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGGGCCGCTGTCGGAGCGTTGCAGCGTCGGCCAGAGAGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACGCGACGCAGGGTGCGCAGGTGGATGACGGTCTCCCCCTCGGTAATCCCCACTGCGTCAGCTACGTg                                                     <  2:240495/251‑1 (MQ=255)                                                                                                                                                                                           gCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGGAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGGGCGCAGGGGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCCGCGACGTGGccc                                                 >  1:270504/1‑250 (MQ=255)                                                                                                                                                                                             tGGCACTATTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGGCCGCG‑AAGTAGTGGGCGATTAAACAGAGCGCGACGCCGGTGACACGACGCAGGGGGCGCAGGTGGATGACGTTCTCCCCCTCGGGAATCCCCCGTGCGGCAGCGACGTGGCCgg                                                >  1:111644/1‑250 (MQ=255)                                                                                                                                                                                              ggCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGa                                               <  1:96597/250‑1 (MQ=255)                                                                                                                                                                                              ggCACGCTTTGGCCGGGGCGCGGCGGGCGCTGAGCCGCTTCTGGCTGCGGTGCAGCGCAATTCCGGTGTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGGCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGGGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGGTCTCCCCCGCGGGAATCCCCAGTGCGTCCGCGTCGTAGCCGGa                                               <  2:374234/250‑1 (MQ=255)                                                                                                                                                                                                cACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCCGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGGAGTGGTCGATTAAACAGAGCGCGACGCCGTTGGCACGACGCCGGGGGCGCCGGTGGATGACGTTCTCCCCCTCGGGAATCCCCCGTGCGTCAGCGCCGGGGCCGGAcgc                                            >  2:78286/1‑251 (MQ=255)                                                                                                                                                                                                cACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGAcgc                                            >  1:84973/1‑251 (MQ=255)                                                                                                                                                                                                     tttGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGGCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGGGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGGAGTGGCCGGACGCggggg                                       >  1:14654/1‑250 (MQ=255)                                                                                                                                                                                                      ttGGCCTGGGCGCGGGGGGCGCTGATCCTCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAAGCCCCAGTGCGTCAGCGACGTGGCCGGACGCgggg                                        <  2:43835/249‑1 (MQ=255)                                                                                                                                                                                                      ttGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAACGCAAATCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGGGAGGTCCGCG‑GAGTAGTGGTCGATTAAACAGAGCGCGCCGCCGTTGAAACCACGCAGGGGGCGCAGGGGGATGACGTTCTCCCCCTCGGGAATCCCCAGTGCGTCCGCGACGTGGCCGGACGCGGGgcg                                      >  1:193297/1‑251 (MQ=255)                                                                                                                                                                                                       tGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGgcg                                      <  1:411238/250‑1 (MQ=255)                                                                                                                                                                                                                cgcgGCGGGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTTCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGGCGGCCAGAGGGGGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAAAGCGCGACGCCGTTGACACGACGCAGGGGGCGCAGGGGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGGCGCGGGGCGCAATACCgg                             >  1:366363/1‑249 (MQ=255)                                                                                                                                                                                                                  cggcggGCGCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGGGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAg                          <  2:192645/251‑1 (MQ=255)                                                                                                                                                                                                                   ggggggtgcTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGc                         <  2:355855/244‑1 (MQ=255)                                                                                                                                                                                                                   ggcgggcgCTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGGCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTGGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGc                         <  1:188181/251‑1 (MQ=255)                                                                                                                                                                                                                           cTGATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTc                 >  2:348166/1‑251 (MQ=255)                                                                                                                                                                                                                             gATCCGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGc               >  1:257514/1‑251 (MQ=255)                                                                                                                                                                                                                                ccGCGTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCtgg            >  1:26782/1‑251 (MQ=255)                                                                                                                                                                                                                                 cgcgTCTGTCTGCTGTGCAGCCCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCCCGGTAATCCCCAGTGCGTCAGCGACGTTGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCtggt           <  1:221208/251‑1 (MQ=255)                                                                                                                                                                                                                                    gTCTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTggg        >  1:308674/1‑251 (MQ=255)                                                                                                                                                                                                                                      cTGGCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGCGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGAt      >  2:413534/1‑251 (MQ=255)                                                                                                                                                                                                                                        ggCTGCGGTTCAGCGCAATCCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTTTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCATGGTTCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGTGTCAGCGACGTGGCGGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGAt      <  1:36576/249‑1 (MQ=255)                                                                                                                                                                                                                                        ggCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGa       <  1:148000/248‑1 (MQ=255)                                                                                                                                                                                                                                         gCTGCGGTGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGc   <  1:2258/251‑1 (MQ=255)                                                                                                                                                                                                                                            gcggtgCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCt  <  1:263142/249‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       GTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCA‑TGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGCAGCGCAATTCCGGTTTGCTCGCGCAGAAAAT‑CGTGCAGCGAGCCGCTGTCGAAGCGTTGCAGCGTCGGCCAGAGGGTGAGGTCCGCG‑AAGTAGTGGTCGATTAAACAGAGCGCGACGCCGTTGACACGACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCT  >  NC_000913/4321646‑4322128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑