Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	400,527	G→A	L133L (CTG→TTG)	ddlA ←	D‑alanine‑‑D‑alanine ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	400,527	0	G	A	100.0%	170.1 / NA	52	L133L (CTG→TTG)	ddlA	D‑alanine‑‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/23);  total (29/23)

CGAACTCGAACGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGC  >  NC_000913/400281‑400764                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              cgaactcgaacGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAAc                                                                                                                                                                                                                                            >  2:332313/1‑250 (MQ=255)       cgaacGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAg                                                                                                                                                                                                                                       <  1:332313/249‑1 (MQ=255)         aacGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAAcc                                                                                                                                                                                                                                   >  1:119732/1‑251 (MQ=255)         aacGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAAcc                                                                                                                                                                                                                                   >  2:16186/1‑251 (MQ=255)                       ggCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAAt                                                                                                                                                                                                                     >  2:309750/1‑251 (MQ=255)                       ggCAATTGAGTACTGTTCTGCACTGGTTACTTGGCTGACACCAACAGAAGAGCCCTGATTAGCCTGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCAGCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAAt                                                                                                                                                                                                                     <  1:190730/251‑1 (MQ=255)                         cAATGGCGTACTTTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTGACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCTTACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAGCGGTAAATTg                                                                                                                                                                                                                   <  2:271062/251‑1 (MQ=255)                                gTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGAccc                                                                                                                                                                                                            >  2:416804/1‑251 (MQ=255)                                      ttcttcACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCgcagc                                                                                                                                                                                                       >  1:337397/1‑250 (MQ=255)                                          tcACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGTCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCAt                                                                                                                                                                                                     <  1:47844/248‑1 (MQ=255)                                           cACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGGGGTAACCCCAGTTTAGACTCCACTTCGGCAACACTAATGTTGAGACGATTGGCGCGCTTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAAACTACAAACGGTAAATTGGCGACCCGCAGCATTccc                                                                                                                                                                                                 >  1:61742/1‑251 (MQ=255)                                            aCTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCt                                                                                                                                                                                                <  1:309750/251‑1 (MQ=255)                                                        gCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTGAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCGTGGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCAt                                                                                                                                                                                    <  1:240553/251‑1 (MQ=255)                                                           gACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGAGTGGCGCGCGTCAGGGTAATAAAGGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCtt                                                                                                                                                                                 <  1:141198/251‑1 (MQ=255)                                                            acacCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTc                                                                                                                                                                                >  1:259638/1‑251 (MQ=255)                                                                 aaCAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCa                                                                                                                                                                           <  1:365714/251‑1 (MQ=255)                                                                          gCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGt                                                                                                                                                                  >  1:107877/1‑251 (MQ=255)                                                                                        gTTTTACAAACAGTGGTAACCACAGTTTAGACTCCACTTAGGCAATACTAATGTTGTGACGCTTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGACAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGac                                                                                                                                                     >  2:209640/1‑249 (MQ=255)                                                                                                 aCAGTGGTAACCCCAGTTTAGACTCCACATCGGCAACACTAATGGTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTGCGCCCAGCGTACCGTGGACAACCGGGAAAATGACATcc                                                                                                                                           <  1:376251/251‑1 (MQ=255)                                                                                                   aGTGGTAACCCCAGTTTAGACTCCACTGCGGCAAAACTAATGTTGTGACGAGTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGGAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCGACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGTGAAAAGGACATCCAc                                                                                                                                         <  2:216073/251‑1 (MQ=255)                                                                                                     tGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTCATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCg                                                                                                                                       >  2:354554/1‑251 (MQ=255)                                                                                                             ccAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCCGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGGGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGGAAATGACATCCACCGTCGGCAAc                                                                                                                               >  1:54796/1‑251 (MQ=255)                                                                                                               aGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACgg                                                                                                                             >  1:89202/1‑251 (MQ=255)                                                                                                                    aGACGCCACTTCGGCAAAACTAATGTTGTGGAGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGGTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGAc                                                                                                                        <  2:233123/251‑1 (MQ=255)                                                                                                                          cACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGtttt                                                                                                                  <  1:133953/251‑1 (MQ=255)                                                                                                                           aCTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTg                                                                                                                 >  2:190658/1‑251 (MQ=255)                                                                                                                               cGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGcgcg                                                                                                             >  1:356645/1‑251 (MQ=255)                                                                                                                                  cAAAAATAATGTTGTGACAATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACCGGCTGCTGAAGCCAAAACATCAGAACCTACAAAAGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGCCATCCACCGCCGGCAACGGCTGACCGTTTTGCGCGTCg                                                                                                          >  2:395497/1‑251 (MQ=255)                                                                                                                                    aaaCTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGAt                                                                                                        <  1:354554/251‑1 (MQ=255)                                                                                                                                        tAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGc                                                                                                    >  1:319981/1‑251 (MQ=255)                                                                                                                                              tgtgACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCg                                                                                                          <  1:58854/239‑1 (MQ=255)                                                                                                                                              tgtgACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCg                                                                                                          >  2:58854/1‑239 (MQ=255)                                                                                                                                                                        ataaATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACCTCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCCTGTTTACCTGGCACCTGCGCacg                                                                    >  1:403172/1‑249 (MQ=255)                                                                                                                                                                         taaATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGAAATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGCCGGCAACGGCTGACCGTTTTGAGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAgg                                                                   >  1:213301/1‑251 (MQ=255)                                                                                                                                                                            aTGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTg                                                                >  1:137207/1‑251 (MQ=255)                                                                                                                                                                              ggCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGt                                                              >  1:53964/1‑251 (MQ=255)                                                                                                                                                                                    aTGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGa                                                        <  1:246419/251‑1 (MQ=255)                                                                                                                                                                                         cAGCCCTGCATTGCGTA‑CACGACGTTTGGTGACATCTGTGTCCATACTGGCTGCTGAAGCCAAAACACCAGAACCTACAAACGGTAAATTGGCGTCCCGCAGCATTCCCTGCACGGAACCATCTGCGCCCAGCGTACCGTGGACAAGCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCTAAGGgc                                                   <  2:154714/251‑1 (MQ=255)                                                                                                                                                                                          aGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGAcc                                                                                                                       >  1:76518/1‑182 (MQ=255)                                                                                                                                                                                          aGCCCGGCATCGCGTAGCA‑GACGTTTGGGGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTTGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGAcc                                                                                                                       <  2:76518/182‑1 (MQ=255)                                                                                                                                                                                                 cagcgcgTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAACACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAAc                                                                                                                               >  2:414847/4‑167 (MQ=255)                                                                                                                                                                                                 cATCGCGTAGCA‑GCCGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGcgcaccgc                                            >  1:65809/1‑250 (MQ=255)                                                                                                                                                                                                 cATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACAGCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAAc                                                                                                                               <  1:414847/167‑1 (MQ=255)                                                                                                                                                                                                         aGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGc                                   <  1:16186/251‑1 (MQ=255)                                                                                                                                                                                                              gACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCACTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGACGGGCGCAACGCAATATGGGCAgg                                >  1:60092/1‑250 (MQ=255)                                                                                                                                                                                                                           aTCTTTGTCCATACAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGAGTACAGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCCCAGAAGGGCGCAACGCACTATGGGAAGGCTCGTCTACAttt                   >  2:127751/1‑250 (MQ=255)                                                                                                                                                                                                                              gttGTCCATACAGGCTGCTGAAGCCCAAACATCAGAACCTGCAATCTGTAAAGTGGCGACCCGCAGCATTCCCTGCAAGGAACCAGCTTCGCCCAGCGTACCGTGGATAATCGGGAAAAGGACATCCACCGTCGGCAACAGCTGAACGTGTTGCGCGTCGAGAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGTGCGCCGAAGGGCGCAACGCAATATGGGCATGATCGGCTGCATTTAGc                <  2:157200/249‑1 (MQ=255)                                                                                                                                                                                                                                  tccatccaGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGACGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGAt            >  2:82408/1‑250 (MQ=255)                                                                                                                                                                                                                                        cagactgctgAAGCCAAAACATCAGAACCTCCAAACGGTAAATGGCCGCCCCGCAGCATCCCCTGCAAGCAACAATCTTCGCCCAGCGTACCGTGGACAATCGGGAAACTGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGAAATCAGGGCAGGATCGTCTGCATTTAGCAGTTAAttact     >  2:35630/5‑248 (MQ=255)                                                                                                                                                                                                                                        cAGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCt     <  2:89202/251‑1 (MQ=255)                                                                                                                                                                                                                                         aGGCTGCTGAAGCCAAAACATCAGAACCTACAAACGGGAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTg    <  2:337397/251‑1 (MQ=255)                                                                                                                                                                                                                                           gctgctGAAGCCAAAACATCAGAACCTACAACCGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGc  <  2:90325/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              CGAACTCGAACGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCA‑GACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTCTGCATTTAGCAGATAATTGCTGGC  >  NC_000913/400281‑400764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑