Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,259,258	G→A	G8S (GGT→AGT)	yjbJ →	putative stress response protein YjbJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,259,258	0	G	A	100.0%	133.0 / NA	37	G8S (GGT→AGT)	yjbJ	putative stress response protein YjbJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/28);  total (9/28)

CATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAGGTAAACGTGATCAACTGGTCGGTAAAATCCAGGAACGTTATGGTTATCAGAAAGATCAGGCAGAAAAAGAGGTCGTGGATTGGGAAACCCGCAATGAATATCGCTGGTAATTAATCCCTCCTGCCCGACGTG  >  NC_000913/4259014‑4259468                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                   cATGCTTTGGGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAAc                                                                                                                                                                                                               <  2:9834/250‑1 (MQ=255)   ggCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGGTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAAGCTCACGTTCAGAAGAAAAGTGAACGTACTCTTATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTgg                                                                                                                                                                                                            <  2:31239/250‑1 (MQ=255)                 cATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAgg                                                                                                                                                                                              >  2:8277/1‑251 (MQ=255)                  aTTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGt                                                                                                                                                                                             <  1:127868/251‑1 (MQ=255)                      aCCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAg                                                                                                                                                                                         <  1:335492/251‑1 (MQ=255)                             ttCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAag                                                                                                                                                                                   <  2:279273/250‑1 (MQ=255)                                   cGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATg                                                                                                                                                                            <  1:329523/251‑1 (MQ=255)                                    gTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCAGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGAGACCGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATACAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTACAGTGAAAGAGCAATgg                                                                                                                                                                           >  2:302709/1‑251 (MQ=255)                                      tGCGCGGGCTTTCTCTGGCGGCTATCGGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGATGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATggg                                                                                                                                                                          <  2:11322/250‑1 (MQ=255)                                            gtcTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACt                                                                                                                                                                   <  1:33173/249‑1 (MQ=255)                                                   cctGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgag                                                                                                                                                            <  2:73176/250‑2 (MQ=255)                                                      ggcggcTATCTGGCGGCGTTACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgatgat                                                                                                                                                         <  1:360836/251‑1 (MQ=255)                                                        cggcTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgatgatg                                                                                                                                                        >  1:227803/1‑250 (MQ=255)                                                             aTCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTTAAAGAGCAATGGGGCAAACTGACCGATGATGatat                                                                                                                                                    <  1:117668/249‑1 (MQ=255)                                                                  gcggcgTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATc                                                                                                                                             <  2:72032/251‑1 (MQ=255)                                                                   cggcgTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACGGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCa                                                                                                                                            <  1:11336/251‑1 (MQ=255)                                                                    ggcgTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCAt                                                                                                                                           <  1:89679/251‑1 (MQ=255)                                                                    ggcgTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCAt                                                                                                                                           >  1:44188/1‑251 (MQ=255)                                                                      cgTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGAGGATGATATGACTATCATTg                                                                                                                                         <  2:260196/251‑1 (MQ=255)                                                                         cACTGGCGCAATTGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGACATGACGATCATTGaa                                                                                                                                       <  2:106210/250‑1 (MQ=255)                                                                              gcgcAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAGGTaaa                                                                                                                                 <  1:385821/251‑1 (MQ=255)                                                                                        aCCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAGGTAAACGTGATCaa                                                                                                                        <  1:76837/250‑1 (MQ=255)                                                                                             gTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAGGTAAACGTGATCAACTGGTc                                                                                                                  <  1:38102/251‑1 (MQ=255)                                                                                                  ccgacacttGACGCTTAAAAATTTTGAATAAATAATACTAAGCCTAATTGCTGACTACACTTAATCTCACGGTCAGCAGAAAAGTGAACGTACTCTCATTCACAACCTAACTATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACGGGAAACATTTTAAAGGTAAAGGGAAAGAGCAATTGGGCAAACTTACCGATGATGATATGACGATCATTGAATGTAAACGTAATCAACTGGTCGGTaa                                                                                                             <  2:28878/243‑1 (MQ=255)                                                                                                           gACGGTTAAAAATTCTGAATAAATAAGCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGAGGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAGGTAAACGTGATCAACTGGTCGGTAAAATCCAGGa                                                                                                    <  2:265176/251‑1 (MQ=255)                                                                                                                           gAATAAATAATCCTAAGCCAAATTGCTTACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAGGTAAACGTGATCAACTGGTCGGTAAAATCCAGGAACGTTATGGTTATCa                                                                                     <  2:11230/250‑1 (MQ=255)                                                                                                                                    atCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAg                                                                                                                                      <  2:265119/192‑1 (MQ=255)                                                                                                                                    atCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAg                                                                                                                                      >  1:265119/1‑192 (MQ=255)                                                                                                                                               aaTTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAGGTAAACGTGATCAACTGGTCGGTAAAATCCAGGAACGTTATGGTTATCAGAAAGATCAGGCAGaacacg                                                                 >  1:191509/1‑246 (MQ=255)                                                                                                                                                   gCTGACTACACTGAATATCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAGGGAAACGTGATCAACTGGTCGGTAAAATCCAGGAACGTTATGGTTATCAGAAAGATCAGGCAGAAAAAGAGGTc                                                            >  2:285424/1‑251 (MQ=255)                                                                                                                                                                        gTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAGGTAAACGTGATCAACTGGTCGGTAAAATCCAGGAACGTTATGGTTATCAGAAAGATCAGGCAGAAAAAGAGGTCGTGGATTGGGAAACCCGCAAt                                       <  1:8277/251‑1 (MQ=255)                                                                                                                                                                        gTTCACAAGACAAGAGAACGTAATCTCATTCACACCCTAACGATGAAGACTAGATTATGACAAAAGATGCCGCCCGCAGTAACTGGAAACAGTTTAACGGTCAAGTGAAAGAGCAACGGGGCAAACTGACCGATGATGATATGACGATCATTGAAGGAAAACGTGATCACCTGGTCGGTAAATTACAGCAATTTTAAGGTTATCAGAAAGAACTGTCAGAAAAAAAGGACGTGGATTGGGataataccatg                                       >  2:8262/1‑241 (MQ=255)                                                                                                                                                                          tCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAGGTAAACGTGATCAACTGGTCGGTAAAATCCAGGAACGTTATGGTTATCAGAAAGATCAGGGAGAAAAAGAGGTCGTGGATTGGGAAACCCGCAATg                                      <  1:115130/250‑1 (MQ=255)                                                                                                                                                                                             aCTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAGGTAAACGTGATCAACTGGTCGGTAAAATCCAGGAACGTTATGGTTATCAGAAAGATCAGGCAGAAAAAGAGGTCGTGGATTGGGAAACCCGCAATGAATATCGCTGGtaattaat                   <  1:1563/250‑1 (MQ=255)                                                                                                                                                                                                          accgaaCGATGAGGTCGTGATTAGGAATAAAGATGAAGCCGGAAGTAACTGGAAATAGTTTAAAGGGAAAGTGAAAGAGCAATGGGGAAAACTGATCGATGATGATATGACGATCATTGAAGGTAAACGTGATCAACTGGTAGGGAAAATCCTGGAACGTTATGGTTATCAGAATGATCAGGCAGAAAAAGAGGTCGTGGATTGGGAAATCCGCAATGAATAGCGATGGTAATTAATCCCGCCTGCCCGAc     <  2:352937/247‑1 (MQ=255)                                                                                                                                                                                                           ccTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAGGTAAACGTGATCAACTGGTCGGTAAAATCCAGGAACGTGATGGTTATCAGAAAGATCAGGCAGAAAAAGAGGTCGTGGATTGGGAAGCCCGTAATGAATATCGCTGGTAATTAATCCCTGCTGCCCGACg    <  2:265048/251‑1 (MQ=255)                                                                                                                                                                                                             tAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCAGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGCTGATGATATGACGATCATTGAAGGTAAACGTGATCAACTGGTCGGTAAAATCCAGGAACGTTATGGTTATCAGAAAGATCAGGCAGAAAAAGAGGTCGTGGATTGGGAAACCCGCAATGAATATCGCTGGTAATTAATCCCTCCTGCCCGACgtg  >  1:4318/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                   CATGCTTTGTGGCTGGCATTAACCGTCTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAGGTAAACGTGATCAACTGGTCGGTAAAATCCAGGAACGTTATGGTTATCAGAAAGATCAGGCAGAAAAAGAGGTCGTGGATTGGGAAACCCGCAATGAATATCGCTGGTAATTAATCCCTCCTGCCCGACGTG  >  NC_000913/4259014‑4259468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑