Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,931,995	G→A	G227E (GGG→GAG)	kup →	K(+):H(+) symporter Kup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,931,995	0	G	A	100.0%	140.3 / NA	48	G227E (GGG→GAG)	kup	K(+):H(+) symporter Kup
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/17);  total (31/17)

ATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCG  >  NC_000913/3931757‑3932237                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   aTTATCGTTCTCACTTTTTTATGTATGATTCAAAAACAGGGCACGGCTATGGTCGGTAAGCTGTGTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTGTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCt                                                                                                                                                                                                                                        <  1:360535/251‑1 (MQ=255)   tATCGTGCTCACTTTATTAGTTATGATTCAAAAACATGGCACGGCTATGGGCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTGTTTGATGCTGGCAGGACTGGTGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAAGGTGGTCGGTGCAGTTCTTCCTTGAATACAAAACGGGGTCGTTTATTGCATTAGGGGCAGTGTTGTTGTCGATTACGGAGGGCGAGGCGCTg                                                                                                                                                                                                                                       <  2:166067/250‑1 (MQ=255)          ctcACTTTCTTATTTATGCTTCCAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCCTTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCCCTGTATGCTg                                                                                                                                                                                                                                >  2:74427/1‑250 (MQ=255)           tcACTTTATAATTTATGATTCAAAAACATGGCACGGCTATGGTAGGTAAGCTGTTTGCGCCGATAATACTGACCTGGTTTTAGATTCTGGCCGGACTGGGGTTACGTAGCATTATTGCTAACCCGGACGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAACACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTCAGGAGGTCGAGGCGCTGTATGCTGat                                                                                                                                                                                                                              >  2:169896/1‑251 (MQ=255)                          ggATTCAAAAACATGGCTCGGTTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGCACTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCGGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTTCGGAGGTCGAGGCGCTGTATGCGGATATGGGGCACtttcgt                                                                                                                                                                                                               <  2:121224/250‑4 (MQ=255)                                   aaCATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGTCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGGTCCCt                                                                                                                                                                                                      <  2:213090/251‑1 (MQ=255)                                   aaCATGGCACGGATATGGTCGGTAAGCTGTTAGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCt                                                                                                                                                                                                      >  1:346211/1‑251 (MQ=255)                                         gCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGc                                                                                                                                                                                                >  2:304115/1‑251 (MQ=255)                                                        gTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCAcc                                                                                                                                                                                 <  1:290752/251‑1 (MQ=255)                                                          aaGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACcgt                                                                                                                                                                               >  1:125358/1‑251 (MQ=255)                                                                           cATGCTGACCTGTTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGTTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCTAGGCGCTGTATGCTGATATGGTGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTg                                                                                                                                                               <  1:246655/250‑1 (MQ=255)                                                                                      ggtttaagATTCTGGCAGGAATGACGTCACGTAGCATTCTTGCTGACCCGGAAGTGATGCCTGCACTGAATCAAATGTGGGCGGTGCATTTCTCCCTTGCATACACAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGCGGCGCTGTACGCTGATATGGGGCACTTTGGTAAGTTCCCTCTTCGCCTGGCGCGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTAc                                                                                                                                                   >  2:249146/8‑251 (MQ=255)                                                                                      ggTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAAtt                                                                                                                                                     <  2:138851/249‑1 (MQ=255)                                                                                      ggTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTAc                                                                                                                                                   >  2:279437/1‑251 (MQ=255)                                                                                          tttGATTCGGGCAGGACAGGGGTTACGTAGCAGTATTGCTAACCCGGAAGGGCGGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTGTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTAGTCGCCTGGCGTTGTTCACCGTCGTATTACCTTCCTGGACCCTTAATTACTTc                                                                                                                                                <  1:96496/250‑1 (MQ=255)                                                                                           ttGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCg                                                                                                                                               <  1:82431/250‑1 (MQ=255)                                                                                             gATTCTGGCAGGACTGGGGTTACGTAGCATTCTTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTc                                                                                                                                            >  2:49574/1‑251 (MQ=255)                                                                                                      aGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAgcgc                                                                                                                                   >  1:195784/1‑251 (MQ=255)                                                                                                               gTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTaa                                                                                                                          <  2:125358/251‑1 (MQ=255)                                                                                                                  aCGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGCATCCAATGTGGGTGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTac                                                                                                                          >  2:405007/1‑247 (MQ=255)                                                                                                                       gCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACGTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCg                                                                                                                  <  1:353005/251‑1 (MQ=255)                                                                                                                         cttattGCTACCCCGGAAGGGATGCGTGAGATGAATCCAATGTGGGCGGTGCATTTCTTACTTGAATACAAACCGGTTTCTTTTATGGCATTGAGGGCCGTGGTGTTTTCGATTCCGGAGGTCGAGGCGCTGTATGCGGAAATGGGGCACTTTGGTAAGTTCCCTATTCGCCTAACGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTGATTACTTCGGGCAGGGAGCGCTGTTGTTAAAGAAccc                                                                                                                   >  2:353588/2‑248 (MQ=255)                                                                                                                                      cGGAAGTGCTGCATGCGCTGAATCCAATGAGGGCGGTGCATTTCCTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCCGTAGCGTTGTCGATTACGGAGGTCGCGGCGCTGTATGCTGATACGGGGCCCTTTAGTAAGTTCACTATTCGCCGGGCGCGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGCTGTTACAGACACCGGAAGCGATTAAGAAc                                                                                                   >  1:410297/1‑251 (MQ=255)                                                                                                                                         aaGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCg                                                                                                >  1:120233/1‑251 (MQ=255)                                                                                                                                         aaGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCg                                                                                                >  1:294571/1‑251 (MQ=255)                                                                                                                                           gTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGtt                                                                                              >  2:208671/1‑251 (MQ=255)                                                                                                                                           gTGCTGCATGCGCTGAATCCAATGAGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTAAGGGAGCGCTGTGGTTACAGAACCCGGACGCGATAAAGAACCCGtt                                                                                              >  2:315610/1‑251 (MQ=255)                                                                                                                                               tgcATGCGCTGCATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTGAGTTCCCTATTCGCCTGGCGTGGTTAACCGTCGTATTACCTTCCTTGACCCGTATTTACTTCGGTCAGGGAGCGCTGTTGTTATAGAACCCGGAGGCGATTAAGAAACCGgtcttc                                                                                          >  2:148925/1‑251 (MQ=255)                                                                                                                                               tgcATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTACGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGttcttc                                                                                          >  2:219947/1‑251 (MQ=255)                                                                                                                                                 cATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAACACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCt                                                                                        >  2:349345/1‑251 (MQ=255)                                                                                                                                                  aTGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGCTTAAGAACCCGTTCTTCCTg                                                                                       >  2:206531/1‑251 (MQ=255)                                                                                                                                                       cTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGc                                                                                  >  1:177028/1‑251 (MQ=255)                                                                                                                                                         gAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGc                                                                                  <  1:266509/249‑1 (MQ=255)                                                                                                                                                          aaTCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCAcc                                                                               <  1:279437/251‑1 (MQ=255)                                                                                                                                                                aTGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTg                                                                         >  1:146456/1‑251 (MQ=255)                                                                                                                                                                    gggCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGgcg                                                                     <  1:17339/251‑1 (MQ=255)                                                                                                                                                                                tcttcCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTGAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCgct                                                          <  1:49574/250‑1 (MQ=255)                                                                                                                                                                                              aaaaCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATcgccgc                                           >  1:413721/1‑251 (MQ=255)                                                                                                                                                                                              aaaaCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGAGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATcgccgc                                           >  2:316671/1‑251 (MQ=255)                                                                                                                                                                                                aaCGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCAc                                         >  1:309979/1‑251 (MQ=255)                                                                                                                                                                                                  cGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGTGCGCTGATCCCGCTGCTGATCATCGCCGCCCTg                                       >  2:319848/1‑251 (MQ=255)                                                                                                                                                                                                   ggTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGGTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTCCCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGAGCCCGCTGCTGATCATCGCCCCACTgg                                      <  2:59329/251‑1 (MQ=255)                                                                                                                                                                                                            tATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCTACGGTa                              <  2:65175/250‑1 (MQ=255)                                                                                                                                                                                                             aTTGCATTAGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAAt                            >  1:413816/1‑251 (MQ=255)                                                                                                                                                                                                                 cATTAGGGGCAGTGGTGTTGTCGTTAAAGGAGGCCGATGCGCTGTCTGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGATTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGTGCGCGCTGTTGTTACAGAACCCGGATGCGATTACGAACCCGTTCTTCCTGCTGGCTCCGGCCTGGGCGCTGATCCCGCTGCTGCTCCTCGCCGCACTgtctagtgtcattgcc                        >  2:148870/1‑236 (MQ=255)                                                                                                                                                                                                                     aGGGGCAGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGc                    >  1:277315/1‑251 (MQ=255)                                                                                                                                                                                                                           aGTGGTGTTGTCGATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCCTCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCgg              >  2:285406/1‑251 (MQ=255)                                                                                                                                                                                                                                       gATTACGGAGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATTTCTGGCg  >  1:267241/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   ATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAGTTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTCGGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTGGCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCCTCGCAGGCGGTTATCTCTGGCG  >  NC_000913/3931757‑3932237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑