Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,914,553	0	A	G	22.9%	122.4 / 11.4	62	H216H (CAT→CAC)	glmU	fused N‑acetylglucosamine‑1‑phosphate uridyltransferase and glucosamine‑1‑phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (11/36);  new base G (14/0);  total (26/36)
Fisher's exact test for biased strand distribution p-value = 1.98e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TAATCACGCAACCGGTGCCAATTTTCACGCGATGACCGAGAGTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGATACGTCCATAACCGGTCGGATCATCCA  >  NC_000913/3914306‑3914803                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                aaaTCACGCAACCGGTGCCAATTTTCACGCGATGACCGAGAGTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGa                                                                                                                                                                                                                                                              <  2:54663/250‑1 (MQ=255)                   cAATTTTCACGCGATGACCGAGAGTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑CCCCTCCTCTTTGC‑TT‑AAACGTTGCGGGTGGAAGGCGACGATTTCatcc                                                                                                                                                                                                                                            >  1:266471/1‑248 (MQ=255)                          cACGCGATGACCGAGAGTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCt                                                                                                                                                                                                                                     <  1:399876/251‑1 (MQ=255)                                     cGAGAGTCACGTTGCCCTCGGTGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGACCGGCGACGATTTCACGCCCTTCCTGATACGCCAgc                                                                                                                                                                                                                          <  2:291400/251‑1 (MQ=255)                                     cGAGAGTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAgc                                                                                                                                                                                                                          <  2:322277/251‑1 (MQ=255)                                      gagaGTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑TTT‑TAC‑GCCCTCTATTTCCG‑TT‑AAAAGTTGGGGATGAACGGCGACGAtttttaccccttccctgtatccca                                                                                                                                                                                                                            >  2:67182/1‑227 (MQ=255)                                        gagTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTATCGTACCACGCAGATCAAAACGCGCTGTATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCTGACTGATAAACACGCTCCAGACGTGAGAGTTGCAGGCGGTT‑AGT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTTATACGCCAGCGCa                                                                                                                                                                                                                       <  1:400629/251‑1 (MQ=255)                                            cACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTTCTTCGC‑TT‑AAACGTTGCGGCAGAACGGCGGCGGTTTTACACCCTTCCTTATACGCCAGCGCcata                                                                                                                                                                                                                    >  1:136843/1‑250 (MQ=255)                                                   cccTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGt                                                                                                                                                                                                            <  1:231591/251‑1 (MQ=255)                                                     cTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CACTGCCT‑CTACTTCGC‑TT‑AAAAGTTGCGGATGAAAAGCGACGACTTTACGCCCTTCCCGGTACGCCAGCGGCATAATCTCGGTgg                                                                                                                                                                                                          >  1:65240/1‑250 (MQ=255)                                                              aaCGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTTGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTTACGCCCTTCCTGATACGCCCGCGCAATAATGTCGGGGATGTAGT‑Att                                                                                                                                                                                                >  1:84681/1‑251 (MQ=255)                                                                       aTCAATTTCGACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAATTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCATGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGTCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGa                                                                                                                                                                                        <  1:188407/250‑1 (MQ=255)                                                                       aTCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGa                                                                                                                                                                                        <  1:17300/250‑1 (MQ=255)                                                                         cAATTTCAACATCGCGCCCGTGAGTTAGCGTCCCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGATAGTTGCATGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGAGACGCCAGCGCAAGAATGTCGGTGATGTTGT‑ATTCGCCCTGAGc                                                                                                                                                                                      <  1:327106/250‑1 (MQ=255)                                                                         acaTTTCAACATCGAGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGTTAACAGCAGTTTTTCAGCCTGTTCGGACTGATCAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑GT‑AAACGTTGCTGATGATCGGCGACGATTTCACGCCCATCCTGATACGCCAGCGCAAGAATGTTGGTGATGTATT‑AGTCTCCCTGAGc                                                                                                                                                                                      <  2:335990/248‑1 (MQ=255)                                                                             ttCATCATCGCGCCCGTGAGTTAGCGTACCACACCGATCAAAACGCGATGGATCGCGCAGCATAACGCCTGCTAACAGCAGTATTTCAGCCTGTTCGGACTGATAAACACGCTCCCGACGGGAGAGTTGCAGACGGTT‑ATT‑CAA‑GCCTTCTACTTCAC‑TT‑AAACGGTGCGGGTGAACGGCGACGCATTCTCGCCCTTCCTTCTACCCCAGCGCCATAATGTCGGTGGTGTAGG‑ATTCACCCTCAGC‑attat                                                                                                                                                                                >  2:267303/1‑251 (MQ=255)                                                                                aaCATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTGGCAGGCGGTT‑ATT‑CAC‑GCCGTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCTAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTAttgt                                                                                                                                                                             <  1:391965/251‑1 (MQ=255)                                                                                       cgcCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑AAT‑CAC‑GCCTTCTACTTACC‑TT‑AAACGTTGCGGGTGAACGGCGACGAATTTACCCCCTTCCTGGTAAGCCAGCCCAATCAAGATGGTGATGGAGG‑ATTTGCCCTGGGG‑ATTAttgtttgac                                                                                                                                                                        >  1:78918/1‑245 (MQ=255)                                                                                           cGTGAGTTAGCGTACCACGCAGAACAAAACGCGCTTGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGGTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTTCTTCGC‑TT‑AAACGTTGCGGATGAACTGCGACGAGTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTc                                                                                                                                                                 <  1:377822/251‑1 (MQ=255)                                                                                            gTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑TTTTCA‑‑GCCTTCTACTTCGC‑TT‑TAAAGTTGCGGATGAACGGCGGCGATTTTACGCCCTTCCTTGTACGCCCGCGCAATAATGTCGGGGGTGGTGT‑ATTCGCCCCGGGC‑ATTATTGTTTGTTAG‑CTTCg                                                                                                                                                                >  1:66560/1‑251 (MQ=255)                                                                                            gTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCg                                                                                                                                                                <  1:284954/251‑1 (MQ=255)                                                                                                    gCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAA‑GCCTTCTACTTCGC‑TT‑TAACGGTGCGGGTGGACGGCGGCGAATTTACGCCCTTCCTGATAAGCCAGCGCCATAAAGTCGGGGGTGGAGT‑ATTTGCCCTGCGC‑ATTATTTTTGGTCAG‑CTTCgccagcc                                                                                                                                                          >  1:296718/1‑249 (MQ=255)                                                                                                                   cAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCTGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTGGCGGATGAACGGCGACGATTTCACGCCCTGCCTGATGCGCCAGCGCAATAATGTCGTGGATGTAGT‑ATTCGCCCTGTGC‑GGTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTGCATATCTgc                                                                                                                                          <  2:30541/250‑1 (MQ=255)                                                                                                                         gcgcTGGATCGCGCAGCATAACGCCTGCTAACAGCAGGTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑g                                                                                                                                  <  2:66560/251‑1 (MQ=255)                                                                                                                              ggATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑TAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGGTGAAAGGCGACGATTTCACGCCCTTCCTGATACGCCCGCGCAATAATGTCGGTGATGTAGT‑ATTTGCCCTGAGC‑ATTTTTGTTTGTCAG‑CTTTGCCCGCCAGCGGTTCATATCTGTGCCCGT‑GGCaa                                                                                                                              >  1:299252/1‑250 (MQ=255)                                                                                                                                agcgcgCAGCATAACGCCTGCTAACAGCAGTTGTTCAGCCTGTTCGGACTGATAAACCCTCTCCAGACGGGAGAGTTGCAGTCTGTT‑ATT‑CAC‑GCCTTCTATTTCGC‑TT‑AAACTTTGCGGATGAACGGCTACTATTTCACGCCCTTCCTTATACGCCAGCGCAATAAGTTCGTTGATGTAGT‑ATCCGCCCTGAGC‑ATTATTGTTGTTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑a                                                                                                                          <  1:384098/249‑1 (MQ=255)                                                                                                                                     gcagcaTAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑TAC‑CCCTTCTACTTCGC‑TT‑TAAAGGTGGGGGTGAACGGCGACGATTTTACGCCCTTTCTGATACGCCCGCGCAATAATGTCGGTGATGTAGT‑AGTTGCCCTGAGC‑ATTTTTGTTGGTTAG‑CTTTGGCAACCCGCGTTTCctattcttgccgttggccatacgaaat                                                                                                                       >  2:107699/1‑228 (MQ=255)                                                                                                                                       agcaTAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGcc                                                                                                                   <  2:115858/251‑1 (MQ=255)                                                                                                                                          aTAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑TAT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGGGGAACGGCCACGATTTTACGCCCTTCCTTATACGCCCGCGCAATAAATTCTGTGATGTAAT‑ATTCACCCCCAGC‑ATTTTTTTTGGTCAG‑CGTCTCCCGCCCGCGGTTTATATCTGCGCCCTT‑TGCAATC‑aagattcccgt                                                                                                                >  1:366506/1‑241 (MQ=255)                                                                                                                                          aTAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGgt                                                                                                                <  1:290764/251‑1 (MQ=255)                                                                                                                                                 cTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGGTGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTTTTGGTCAG‑CTTCGCCAGCCCGCGGTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGAtc                                                                                                         >  1:103007/1‑251 (MQ=255)                                                                                                                                                   gCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCATACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGAtct                                                                                                        <  1:233272/250‑1 (MQ=255)                                                                                                                                                    cTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GACTTCTCCTTCGC‑TT‑AAACGTTCCGGGTGCACCGCGCCGATTTCACGCCCTTCCCGATACGCCAGCGCCATAATGTCGGTGATGTAGT‑ATTCGCCCTCAGA‑ATTATTGTTGGTCAG‑CCTCCCCAGCCCGCGTTTCATATCTGTGCCCTT‑GGCCATC‑AGAAAGCCGGTGTTGATCTcc                                                                                                      >  1:159689/1‑251 (MQ=255)                                                                                                                                                      aaCAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCCTTTACTTCGC‑TT‑AAACGGTGCGGATGAACGACGGCGGTTTCACGCCCTTCCTGATACGCCAGCGCCATAATTGCGGTGGTGTAGT‑ATTTACCCCGGGC‑AATATTTTTTGTCAG‑CTTCGCCACCCAGCCGTTCATATCTTCGCCGTT‑GGCAATT‑AGAAAGCCGGGGGTGATCTCCTg                                                                                                    >  1:75876/1‑251 (MQ=255)                                                                                                                                                                    aGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGATCTCCTGAATCTGACgctgct                                                                                      <  2:103007/251‑1 (MQ=255)                                                                                                                                                                     gCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTTGG‑TT‑AAAAGGTGGGGGGGGACGGCGACGATTTTAAGCCCTTTCTGGTACCCCCGCGCAATAATGTCGGGGGTGTAGT‑ATTCGCCCTGGGCCATT‑TTGTTGGTCCA‑CTTCGCCCGCAAGCGGTTCCTATCTGCCCCGGT‑GGCAAAC‑AGAATTCCGGGGTTGATCTCCTGGATCTGACGCTGCtc                                                                                     >  1:65622/1‑251 (MQ=255)                                                                                                                                                                        tGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTTCTTT‑CA‑‑GCCTTCTACTTCGC‑TT‑AAACGGTGCGGATGAACGGCGACGCTTTCACCCCCTTCCCTATACGCCAGCGGAATAATGTCGGGGCTGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CCTCGCCAGCCCGCGTTTCATACCTTCGCCGTT‑TGCAATC‑AGAATGCCGGGGTTGATCTCCTGAATCTGACGCCGCccgtc                                                                                  >  1:346079/1‑251 (MQ=255)                                                                                                                                                                        tGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGGT‑TTT‑CAC‑GACTTCTACTTAGCCTT‑AAA‑GGTGCGGGTGGACGGCGACGATTTCACGCCCTTTCTGATACGCCCGCGCAAAAATTTTGGGGGTGGAGT‑ATTCGCCCTGCGC‑ATTATTTTTTGTCAAATTTCCCCGGCCGGCGTTTCAT‑TCCTTGCCGTT‑GGGAATC‑CGAATTCCGGTGTTGATTTCCTGTATCTgtcactgcgctgc                                                                                  >  2:150028/1‑239 (MQ=255)                                                                                                                                                                         gTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCtcgtc                                                                                  <  1:162980/250‑1 (MQ=255)                                                                                                                                                                         gTTCGGACTGAGAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACTCCAGCTCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGTTGTTGATCTCCTGTATCTGACGCTGCtcgtcg                                                                                 <  2:266471/251‑1 (MQ=255)                                                                                                                                                                           tcgaaCTGATAAACACGCTCCAGGCGGGAGAGTTTCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCTCCCTGAGC‑ATTATTGTTGTTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCgg                                                                                <  2:220793/246‑1 (MQ=255)                                                                                                                                                                            cGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTg                                                                              <  1:173384/251‑1 (MQ=255)                                                                                                                                                                               aCTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑CACTTCTACCTCGC‑TT‑TAACGTTGCGGGTGGACGGCGGCGAATTCACGCCCTTCCTGATACGCCAGCGCAATAATGGCGGGGGTGGAGG‑ATTCGCCCTCAGC‑GTTTTTTTTGGTCAG‑CTTCGCCCGCCCGCGGTTCATATCTGCGCCCTT‑GGTAATC‑AAAATGCCGGGGGTGGTCTCCCGAATCCGTCGCTGGTCGGCGGTgggc                                                                           >  2:228880/1‑249 (MQ=255)                                                                                                                                                                                     aaaCACGCTCCAGACGGGAGAATTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGA‑TT‑AAACGTTGGGGGGGACCGGCGGAGATTTCACGCCCTTTCTTATACGCCAGCGCAATAATGTCGGTGATGGAGG‑ATTCGCCCCGGGG‑CTTATTGTTGGTCAG‑CTTTGGCCGCCCGAGTTTCCTATCTTCTCCGTT‑GGCAATCCAGC‑TGCCGGGGTTGCTCTCCTGGATGTGCCGCTGCTCGCCGGGGGGATCtttt                                                                     >  2:134441/1‑250 (MQ=255)                                                                                                                                                                                       acacGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTtgtg                                                                   <  1:64530/251‑1 (MQ=255)                                                                                                                                                                                       acacGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGAGCTCCTGAATCTGACgctgct                                                                                      <  1:304740/232‑1 (MQ=255)                                                                                                                                                                                        cacGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAA‑GCCTTCTACTTCGC‑CT‑AAAAGGTGCGGGTGAACAGCGGCGGTTTCACGCCCTTCCCGGTAAGCCCGGGCAATAATGTCGGGGATGGAGT‑ATTTGCCCTGAGC‑ATTTTTTTTGGTCCG‑CTTCTCCAGGCAGCGTTTTATATTTGCCCCGTTTGGCA‑TC‑AGAATGCCGGGGTTGGTCTCCTTTATTTGCCGCTGCTCGTCGGGGGCGTCttttttt                                                                  >  1:166268/1‑247 (MQ=255)                                                                                                                                                                                             tCCAGACGGGCGAGTTGCAGGCGGTT‑ATT‑TAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTTACGCCCTTCCCGATACGCCAGCGCCAAAATGTCCGTGGTGGAGG‑ATTCCCCCCTAGC‑CTTTTTTTTGGTCAG‑CTTCCCCAGCCAGGGGTTCATATCTGCGCCCTT‑TGCAATC‑AAAAAGCCCGTGGTGATCTCCTTGATTTGGCGCGGCTCGTCGGTGGCATCtttttggtcca                                                              >  1:104448/1‑242 (MQ=255)                                                                                                                                                                                              ccAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCTC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CGTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTcaa                                                              <  2:336241/249‑1 (MQ=255)                                                                                                                                                                                              ccAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTcaac                                                             <  1:4462/250‑1 (MQ=255)                                                                                                                                                                                              ccAGACGGGAGAGGTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGGTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTcaac                                                             <  2:340494/250‑1 (MQ=255)                                                                                                                                                                                                   cGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGcc                                                       <  1:325083/251‑1 (MQ=255)                                                                                                                                                                                                    gggAGATTTGCAGGCGGTT‑AAT‑CAC‑GCCTTTTACATTGC‑TTTAAAGGTTGCG‑ATGGAAGGGGGCGCTTTCACGCCCTTCCTGATAACCCAGCGCAATAATGTCGGGGGGGTCGTGATTC‑CCCCGAGCCATT‑TTGTTTGTCCA‑CTTCGCCAGCCAGCGTTTCCTCTCTGCGCCCGT‑GGCGATC‑AGCATTCCGGTGTTGGTCTCCTTAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTcaccacagccg                                                      >  2:96963/1‑242 (MQ=255)                                                                                                                                                                                                         ggtggCAGGCGGTT‑ATT‑CAA‑GCATTCTGCTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGGGATGTAGT‑ATTCGCCCTGAGC‑ATTAGTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCGGCGCCGTT‑GGCAAGC‑AGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCGTTGTGCTCAACAATGCCGGTAAc                                                 <  2:7480/247‑1 (MQ=255)                                                                                                                                                                                                              cAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGc                                            >  1:26888/1‑251 (MQ=255)                                                                                                                                                                                                                       taat‑CAC‑GTCTTCTACGTCGC‑TT‑AAATGGTGCGGATGAACGGCTACGATTTCACGCCCTTCCTTATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCTGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTTACATCTTTGTTCTCAACAATGCCTGTATCTGTGCCTTTTTc                                     <  1:339820/247‑1 (MQ=255)                                                                                                                                                                                                                        aTT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATGCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCAc                                   <  2:84681/251‑1 (MQ=255)                                                                                                                                                                                                                              c‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTg                              <  1:149605/251‑1 (MQ=255)                                                                                                                                                                                                                                    tCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGGCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTTGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGATAc                          <  1:295309/250‑1 (MQ=255)                                                                                                                                                                                                                                                    aCGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGATACGTCCATAACCGGTCg           <  1:143248/251‑1 (MQ=255)                                                                                                                                                                                                                                                          cGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGATCTCCTGAATCTGCCGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGATACGTCCATAACCGGTCGGatcat     >  2:194787/1‑251 (MQ=255)                                                                                                                                                                                                                                                            gATGAACGGCGAGGATTTCACGCCCTTCCTGATACGCCGGCGCACTAATGTCGGTGATGTCGT‑ATTCGCTCTGCGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCCGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGCGTGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGCGGCATATTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCCCGGGTGCTTCGTACCTAACCGGTCGGgtcagcc   >  2:393641/1‑248 (MQ=255)                                                                                                                                                                                                                                                             aTGAACGGCGCCGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGATACGTCCATAACCGGTCGGATCATCCa  <  2:142073/251‑1 (MQ=255)                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                TAATCACGCAACCGGTGCCAATTTTCACGCGATGACCGAGAGTCACGTTGCCCTCGATGATAACGTTAGTATCAATTTCAACATCGCGCCCGTGAGTTAGCGTACCACGCAGATCAAAACGCGCTGGATCGCGCAGCATAACGCCTGCTAACAGCAGTTTTTCAGCCTGTTCGGACTGATAAACACGCTCCAGACGGGAGAGTTGCAGGCGGTT‑ATT‑CAC‑GCCTTCTACTTCGC‑TT‑AAACGTTGCGGATGAACGGCGACGATTTCACGCCCTTCCTGATACGCCAGCGCAATAATGTCGGTGATGTAGT‑ATTCGCCCTGAGC‑ATTATTGTTGGTCAG‑CTTCGCCAGCCAGCGTTTCATATCTGCGCCGTT‑GGCAATC‑AGAATGCCGGTGTTGATCTCCTGAATCTGACGCTGCTCGTCGGTGGCATCTTTGTGCTCAACAATGCCGGTAACTTTGCCGTTTTCACGGGTGATACGTCCATAACCGGTCGGATCATCCA  >  NC_000913/3914306‑3914803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑