| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,173,363 | 0 | C | . | 75.0% | 45.5 / 22.4 | 24 | intergenic (‑490/+919) | gatD/gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
| Reads supporting (aligned to +/- strand): ref base C (1/5); new base . (11/7); total (12/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACG > NC_000913/2173125‑2173601 | attggccgccTGGTGGGTGTGAAAACCAAGAGTTTGCGTGGCGATCCACAGGGTGATGCTCATAATGAAGACACCGGAGATTAAGGTGCGGAACAGAGTCCCCCGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGAGCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATAAAAATGGTGAGTGGGATAAAAATCAGGCTCGCCGATACCACC‑‑GCCGTATGTCCca < 2:131755/247‑1 (MQ=255) ttGGTTGTGAAAACCAATAATTGGCGTAGCGATCCCCAGTTTGATGCTCAAAAGGCTGACCCCCGACATTAAGGTGCGAAACACATTCCCCCGATGCACGGCGACCGCCATCACCACGAATAAGCCAATGATGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACG‑GCAATTAAAATGTTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGcc < 2:307/250‑1 (MQ=255) ccgcgATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGAGGCACGGCGCCCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGa < 1:227845/250‑1 (MQ=255) ccACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACACACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgccg > 2:111897/1‑250 (MQ=255) tGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGcc > 1:39204/1‑251 (MQ=255) ccccGAGATTAAGGTGCGGAACAGATTTCCACGAGGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCt < 1:4211/250‑1 (MQ=255) aaggggcggacaataTTTCCACGAGGCACGGGGACCGCCACCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt < 1:9427/237‑1 (MQ=255) gggaagagttttCCACGATGCAGGGGGACCGCCACCGCCACGAAGAAGCCTATGGTGGCAAGAGCGCCAAACGGCGGCGCCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGGGAGGGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCGGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGt < 1:215759/242‑1 (MQ=255) aGATTCCCACGATGCACGCCGCCCGCCATCGCACCGAAGAAGCCGATGTTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGTCCCACAAACC‑GCAATTAAAATGGTGATGGGGATAAAACTCAGGCTTGCCGAT‑CC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGTCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAcc < 1:393253/251‑1 (MQ=255) cccgagccACGCCGACCGCAACCGCCAGGAAAAAGCCGATGGTGGAAAGATCCCCAAACGGCAGCACCTGATTCCCCGGCACACAAACA‑GAAATTAAAATGGTGAGTGGGATAAAAATCAGCCTTGCCAATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatg < 2:347801/244‑1 (MQ=255) agCCACGAAAACGCCGATGGGGGCATGATCCCCAAACGGCAGCACCTGAGTACCCGGCACAAAAAGA‑GCAATTAAAAGGGTAAGTGGAATAAAAGACATTTGTCACGATCCGACC‑‑GCCGTATGTCCAAGCAGCAACGCCGGGTCAAGGCCAATAAGGAACTCCTGACCGCCGAACTTCGCCTGGAAGCGGCTACTAGCCTGCGTAGCGACGTGCGTTAAACCAGCTAGGATGGGTTTAAGCACCCGTGGCa < 2:406306/250‑1 (MQ=255) gaagaaGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGGCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGCACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTACTCACCCGTGGCATTagc > 2:131558/1‑250 (MQ=255) ggATGGTGGCAAGATCGCAAAACGGCACCACCTGATTCCCCGGCAAACAAACA‑ACAATTAAAATGTGGAGTGGAAAAAAAACAAGGCTGGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACt < 2:143025/250‑1 (MQ=255) aTGGTGGCAAGTTCGCCACACGGCAGAACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGCGCGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCCTACATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTgc > 2:157627/1‑251 (MQ=255) gATCGCAAGACGGCGGCACCTGAT‑ACCCCGGACCCCAACAAGCAATTAAAATGGTGGGTGGTATAAAAATCAGGTTTGC‑GAATCCACCC‑GCCGTATGTCCCAGCAGAAACGCCGTATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGATGACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGTGTTTAATTACCCGTGGCATTAGCAGCATCACTGCCGCCGttttt < 2:294674/251‑1 (MQ=255) cAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAACTCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCACGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCAACCATGATGGGTTTAATCACCCGTGGCATTAGCAGAATCACTGCCGCCGTTTTTACCGcc > 2:96926/1‑251 (MQ=255) aCGGCAGCACCTGATTACCCGGCACACACACA‑GCAATTAAAATGGTGAGTGGGATAACAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTACTCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGc > 2:211542/1‑251 (MQ=255) tGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATA‑‑ACACCGCCGTATGTCACAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACAGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTACCGCCGTTTTTCACGCCAGCTGCAATACAcc > 2:216523/1‑251 (MQ=255) gATTACCCGGCACACAAACA‑GCAATTAAAATGGAGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCACTCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCt > 1:244764/1‑251 (MQ=255) ccA‑GCAATTAAAAGGGTGAGTGGGATAAAAACCAGGCTTGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCcgc < 1:131558/250‑1 (MQ=255) cA‑GCAATTAAATTGGTGCGTGCGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGCTCAAGGCAAATCAGGAACTCCTGACCGCCGAAATTCGCCTGTAAACGACTAAGAGCCTGCTTAGCGATGGGCGTTAAACCATCAATGATGGGTTTAATCACCCGTGGAATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGATGCAATACACCTTTTACATCGTAACAcgcg > 2:287804/1‑251 (MQ=255) gAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATc > 1:158542/1‑250 (MQ=255) caTCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCCGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATcaca > 2:79180/2‑250 (MQ=255) ttGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACg > 2:107074/1‑251 (MQ=255) | ATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACG > NC_000913/2173125‑2173601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |