Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	809,217	2 bp→AG	coding (40‑41/1290 nt)	bioA ←	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	809,217	0	C	A	100.0%	160.9 / NA	49	W14M (TGG→CTG)	bioA	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/24);  total (25/24)
*	NC_000913	809,218	0	A	G	100.0%	177.8 / NA	49	W14M (TGG→CTG)	bioA	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/24);  total (25/24)

AGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCG  >  NC_000913/808974‑809456                                                                                                                                                                                                                                                    ||                                                                                                                                                                                                                                               aGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCCGAGGGGAGGTCATGGATGTGTCTGGGTGCAGGATATg                                                                                                                                                                                                                                          >  2:182919/1‑251 (MQ=255)          cacaGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAACTCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGCTGTGTATGGGTGCAGGATATGGCGTTggac                                                                                                                                                                                                                                 >  1:227697/1‑249 (MQ=255)          cacaGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTc                                                                                                                                                                                                                                 >  1:275876/1‑251 (MQ=255)          cacaGCTCAAAGGCAGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATATGCGCCTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTAAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAACCCGGCAGAGGGGAGGACATGGATGTGAATGGGAGCAGGATATGGCGTTGGTc                                                                                                                                                                                                                                 >  2:213596/1‑251 (MQ=255)                 cAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGc                                                                                                                                                                                                                           <  2:65318/250‑1 (MQ=255)                   aTGGCTGGAGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATAGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGAGCAGGATATGGCGTTGGTCAAAGGCa                                                                                                                                                                                                                          >  1:278592/1‑249 (MQ=255)                      gctggcGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAAATCGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCACCCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGGGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATc                                                                                                                                                                                                                     <  2:21484/251‑1 (MQ=255)                              aTGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTg                                                                                                                                                                                                             >  1:470881/1‑251 (MQ=255)                                       aCCGCCAAACATCACATGCGACATGGCATCAATTTGAGACTTCATCGCCGCATTAAGCTGCGGGTGATTGAAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCGGAGGGGAGGTCACGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGAAATAATCg                                                                                                                                                                                                    >  2:433492/1‑251 (MQ=255)                                          gCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACt                                                                                                                                                                                                 <  1:138653/251‑1 (MQ=255)                                           ccAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACtt                                                                                                                                                                                                <  1:347961/251‑1 (MQ=255)                                                     caTGCGACATGGCATCAATTTGCGACTTCATCGACGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCAACGGATAAACCGGCAGAGGGGCGGTAATGGATGTGTATGGGGGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAAACaa                                                                                                                                                                                       >  1:304355/1‑250 (MQ=255)                                                      aTGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAAt                                                                                                                                                                                     >  1:311337/1‑251 (MQ=255)                                                      aTGCGACATGGCATAAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGAATCGCCGCCCACCAGGACGCCATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGCGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCAGTTGTCATAATCGACTTGTAAACCAAAt                                                                                                                                                                                     >  2:449731/1‑251 (MQ=255)                                                        gCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAAtt                                                                                                                                                                                    <  1:264479/250‑1 (MQ=255)                                                            cATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGaaaa                                                                                                                                                                               <  2:275876/251‑1 (MQ=255)                                                             aTGGCATCAATTTGCGACTTCATCGCCGCATTAAGCGGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAg                                                                                                                                                                              <  2:432053/251‑1 (MQ=255)                                                                    cAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAgg                                                                                                                                                                       >  1:456142/1‑251 (MQ=255)                                                                      aTTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGTACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATACACCGGCGGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGGTCGTCCGTTGACATAATCGACTTGTAACCCAACTTGCAACGATTTAGGtt                                                                                                                                                                     >  1:255099/1‑251 (MQ=255)                                                                         tGCGACTTCATCGACGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTAc                                                                                                                                                                  >  2:352009/1‑251 (MQ=255)                                                                           cGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACa                                                                                                                                                                 >  2:272958/1‑250 (MQ=255)                                                                           cGACGTCATCGCCACATTAAGCGGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCTTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGTGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAATATTTAGGTTTACaa                                                                                                                                                                <  2:20349/251‑1 (MQ=255)                                                                                       cgcATTAAGCTGCGGGTGATTGTAGTCGTGGCTCGCCTCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCGTCGGCGCTCACCACCGTATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGTAAGATCGTCCGTTGTCATAATCGACTTGTAATCTAAATTGAAAATATTTAGGTTTACAAGTTTACACCGaa                                                                                                                                                    <  2:461820/251‑1 (MQ=255)                                                                                          aTTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAAtt                                                                                                                                                  >  1:399731/1‑250 (MQ=255)                                                                                               gCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACAGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTaacaac                                                                                                                                            >  1:482874/1‑251 (MQ=255)                                                                                                   cGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGCCGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATAGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACaaa                                                                                                                                         >  2:206856/1‑250 (MQ=255)                                                                                                         aTTGTAGCCGTGGCTCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGGCTGCCGTCAGACAAAATCAGCGCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGTGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACg                                                                                                                                  <  2:281133/251‑1 (MQ=255)                                                                                                         aTTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACg                                                                                                                                  >  2:399132/1‑251 (MQ=255)                                                                                                                            gccCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATgg                                                                                                               <  1:322892/251‑1 (MQ=255)                                                                                                                              ccaccaGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCt                                                                                                             >  1:14640/1‑251 (MQ=255)                                                                                                                               caacaGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCCGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACAGAATTAACAACAAAAAACACGTTTTGGAGACGCCCCATGGCTc                                                                                                            >  2:445382/4‑251 (MQ=255)                                                                                                                                                  cAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACAtt                                                                                         <  1:206856/251‑1 (MQ=255)                                                                                                                                                    aCCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGt                                                                                       <  2:130448/251‑1 (MQ=255)                                                                                                                                                    aCCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGt                                                                                       <  1:420974/251‑1 (MQ=255)                                                                                                                                                    aCCAGGCGTCCGCCGTCAGACAAAAGCAGCTCGCAACCTGCGGCGCTCACCTCCGGATAAACCGGCAGAGGGGAGGTCATGGATGGGTATGGGTGAAGGATATGGCGATGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTTACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGt                                                                                       <  2:142519/251‑1 (MQ=255)                                                                                                                                                     ccAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGtt                                                                                                                                                                                                                                     >  1:115369/1‑108 (MQ=255)                                                                                                                                                     ccAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGtt                                                                                                                                                                                                                                     <  2:115369/108‑1 (MQ=255)                                                                                                                                                                 cGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGa                                                                          <  2:156868/251‑1 (MQ=255)                                                                                                                                                                      gACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGGCGCAAGTCACAGAattat                                                                     <  1:388173/251‑1 (MQ=255)                                                                                                                                                                                 cTCGCCACCTTCGGCGCTCACCACCGGATAAACCGGCAGCGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCCCGCTGGACATTGTCGCCAGTCACAGAATTATTTGAAAACCCg                                                          >  2:75686/1‑251 (MQ=255)                                                                                                                                                                                      aaCCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGAGCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCt                                                     <  2:178966/251‑1 (MQ=255)                                                                                                                                                                                              gcgcTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATctgc                                             <  2:187000/251‑1 (MQ=255)                                                                                                                                                                                                 cTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATAGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACACGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACCTTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGt                                          >  2:139661/1‑251 (MQ=255)                                                                                                                                                                                                      caccGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTACACCAAATTGAAAAGATTTAGGTTTACAAGTCTACAACGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGATCATTGTCGCAAGTAACAGAATTATTTGAAAAACAGTTGATGGATCAGCTGTTTGa                                      >  2:5741/1‑250 (MQ=255)                                                                                                                                                                                                       accGGATAAACCGGAAGAGGGTAGGTCATGGATGTGTATGGGTGCAGGATATGTCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTTCACCGAATTAACAACAAAAAACTCGTGTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGTCATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTTTTTGAAg                                    <  2:386250/251‑1 (MQ=255)                                                                                                                                                                                                         cGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAgcg                                  <  1:389387/251‑1 (MQ=255)                                                                                                                                                                                                                            gAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGccggc               <  1:352790/251‑4 (MQ=255)                                                                                                                                                                                                                                tCATGGAAGAGTATGGGTGCAGGATATGGCGTTGGTCAACGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGGATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCAttt           >  2:78418/1‑251 (MQ=255)                                                                                                                                                                                                                                          gtATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCg  <  2:130346/250‑1 (MQ=255)                                                                                                                                                                                                                                                    ||                                                                                                                                                                                                                                               AGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTCG  >  NC_000913/808974‑809456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑