Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	367,573	G→A	intergenic (‑63/+14)	lacI ← / ← mhpR	DNA‑binding transcriptional repressor LacI/DNA‑binding transcriptional activator MhpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	367,573	0	G	A	100.0%	142.7 / NA	48	intergenic (‑63/+14)	lacI/mhpR	DNA‑binding transcriptional repressor LacI/DNA‑binding transcriptional activator MhpR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/27);  total (21/27)

TGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTT  >  NC_000913/367332‑367796                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                gcttggCCCGCGAGTTGGTGTTGCACGGGGTTGGGAATGTAAGTCAGCACCGCAATCGCCGCTTCCTCTGTTGCACGCGTGATCGCAGAAACGTGGCTGGTCGGGTTCACCACGCGGGAAACGGTCTGATAAGAGAGACCGGCATACTCTGCTACATCGTATAACGTTACTGGTTTCACATTCACTACCCTGAATTGACTCTCTTCCGGGCGTTATAATGCCATACCGCGAAAGGTTTTGCACCATTCGAt                                                                                                                                                                                                                        <  2:243981/246‑1 (MQ=255)      gCCCGCCTGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgtg                                                                                                                                                                                                                    <  2:326917/250‑1 (MQ=255)         cGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAAGTCAGCTCCTCCATCGCCGCTTCCCCTTTTTCCCGCGTTTTCGCAGAAACGGGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTGGCTCATTCACCACCCTGAATTGACTCTCTTCCTGTCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGgtggc                                                                                                                                                                                                                  <  1:208504/249‑3 (MQ=255)                tgttgcGCCACGCGGTTGGGCATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAat                                                                                                                                                                                                         >  2:257410/1‑251 (MQ=255)                    gtgCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCTCTTCCCCTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGGTCCCCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACAGCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTGCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAatgcat                                                                                                                                                                                                     <  1:22725/251‑1 (MQ=255)                    gtgCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCCTACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAatgcat                                                                                                                                                                                                     >  2:117286/1‑251 (MQ=255)                           gcgGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACGTTTTCCCGCGTTTTCTCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCTTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAATGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCtt                                                                                                                                                                                              <  1:110809/251‑1 (MQ=255)                           acgccgccctcccgatctCAGCGCCGCCATCGCCGCTTCCACTGTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCtt                                                                                                                                                                                              <  2:249637/234‑1 (MQ=255)                             ggTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATACGAGACACCGGCATACTCTGCGACATCGTATAACGATACTGGTTTCACATTCACCACCCTGAATTGAATCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCg                                                                                                                                                                                            >  2:437625/1‑251 (MQ=255)                                  ggAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCCAGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACCTTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCATTc                                                                                                                                                                                       >  1:334326/1‑251 (MQ=255)                                  ggAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTc                                                                                                                                                                                       >  1:262123/1‑251 (MQ=255)                                          aTTCAGCTCCGCCATCGCCACTTCCACTTTTTCCCGCGTTTTCGCAGCCACGAGGCTGACCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCAGCATACTCTGCGCCCTCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATAATGCCCTACCGCGAAAGGTTTTGAACCATTCGATGGTGTCCACGTAAATGCCTGACGCTTCGCCTTCCGGacacc                                                                                                                                                                               >  2:437738/1‑251 (MQ=255)                                             cAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCtt                                                                                                                                                                                              >  1:249637/1‑233 (MQ=255)                                                 cccGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAg                                                                                                                                                                        <  1:222007/250‑1 (MQ=255)                                                   cGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGcc                                                                                                                                                                      >  2:26719/1‑251 (MQ=255)                                                     ccATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTg                                                                                                                                                                    <  1:507406/251‑1 (MQ=255)                                                         cgccgcTTCCACTTTTTCCCGCGTGTTCGCAGAAACGTGGCTGGCCTGGTGCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACAGCGTATAACGTTACTGGTTTCACATGCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGAt                                                                                                                                                                <  2:196071/251‑1 (MQ=255)                                                          gccgcTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGAtt                                                                                                                                                               <  1:105798/251‑1 (MQ=255)                                                             gcTTCCACTTTTTCCCGCTTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGTCATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCaa                                                                                                                                                            <  1:468505/251‑1 (MQ=255)                                                                       tatCCCGCGTTTTCGCCGAACCGTGGCTGGCCTGGTTCCCCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGTTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCcttctt                                                                                                                                                   <  2:7309/248‑1 (MQ=255)                                                                             gcgTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACGCCGGCATACCCTGCGACATCGTATAACTTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTTAACGTAAATGCATGCCTCTTCGCCTTCCTGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTg                                                                                                                                            <  2:335161/251‑1 (MQ=255)                                                                                    cGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCt                                                                                                                                     >  2:332966/1‑251 (MQ=255)                                                                                             gTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCGCCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGt                                                                                                                            <  1:307772/251‑1 (MQ=255)                                                                                               ggctggcCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGCCACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCAGACCGCGAAAGGTTTTGCACCAGTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACGAGAAGTGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAg                                                                                                                          <  1:30535/251‑1 (MQ=255)                                                                                                    gcCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCg                                                                                                                     >  1:19618/1‑251 (MQ=255)                                                                                                                acGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCtttt                                                                                                         <  2:138934/251‑1 (MQ=255)                                                                                                                   cgGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGACAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGTTCTGTTTTGCTACCCGTTGTAGCGCCGGCAGATGCTTTTCCg                                                                                                      >  2:5433/1‑251 (MQ=255)                                                                                                                    gggAAACGGTCTGATAAGAGACACCGGCATACTCTGCGGCATCGTATACCGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTGCTTCGAGCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGc                                                                                                     <  2:372173/251‑1 (MQ=255)                                                                                                                         ccGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATCCTCCCCCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCt                                                                                                <  1:489354/250‑1 (MQ=255)                                                                                                                           ggTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTGTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGt                                                                                              <  1:136388/251‑1 (MQ=255)                                                                                                                                gATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAACTGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGGTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAAt                                                                                         >  1:367223/1‑251 (MQ=255)                                                                                                                                gATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAAt                                                                                         <  1:271681/251‑1 (MQ=255)                                                                                                                                 aTAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATg                                                                                        >  1:361286/1‑251 (MQ=255)                                                                                                                                    agagACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTc                                                                                     >  1:279731/1‑251 (MQ=255)                                                                                                                                       gaCACCGGCATACTCTGCGGCATCGTATAACGTTACTGGTTTCACATGCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCAGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCAtt                                                                                  <  1:223011/251‑1 (MQ=255)                                                                                                                                                aTACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCTTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGcc                                                                         >  1:185075/1‑251 (MQ=255)                                                                                                                                                  aCTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCat                                                                       <  1:112351/251‑1 (MQ=255)                                                                                                                                                       gCGACATCGTATAACGTTCCTGGTGTCACATTCACCACCCTGTATTGACTCTCTACCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCGTCGATCTGTTTTGCTTCCCGGTGTAGCTCCGGAAGATGCTTTTCCTCTGCCTGTTCAATGGTCATTGCGCTCGCCATATaca                                                                  <  2:332286/251‑1 (MQ=255)                                                                                                                                                       gCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCACTGGTCATTGCGCTCGCCATATac                                                                   >  1:491458/1‑250 (MQ=255)                                                                                                                                                                 ataACGTTACTGGTTTCCCATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTAGCTCTGTTTTGCTACCCGTTGTAGCGCCGGAGGATGCTTTTCCGTTGCCTGTTCAATGGACATTGCGCTCGCCCTATAAACCAGATTc                                                          >  2:39257/1‑249 (MQ=255)                                                                                                                                                                                       caccacCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATGCGATGGTGTCAACTTAAATTCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTGCTGCGATCTGTTTTGCTACCCGTTGTATCGCCGGAAGATGCTTTTCCGCTTCCTGTTCAATGGTCATTGCGCTCTCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCa                                  <  2:491458/251‑1 (MQ=255)                                                                                                                                                                                               tGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACAATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTgcgc                            >  1:28318/1‑249 (MQ=255)                                                                                                                                                                                                     gACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTAc                    >  2:273396/1‑251 (MQ=255)                                                                                                                                                                                                         cgctTCCGGGCGCTATCATGCCATACCGCGAAATGTTTTGCACCATTCGATGGGGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTGTTTCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATTCACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCg                <  1:299726/249‑1 (MQ=255)                                                                                                                                                                                                             tCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATa             <  1:439979/250‑1 (MQ=255)                                                                                                                                                                                                              ccGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATaga           <  1:378344/251‑1 (MQ=255)                                                                                                                                                                                                                gggCGATATCATGCCATACCGCGAACGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGACGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCACTCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAgg         >  2:29539/1‑251 (MQ=255)                                                                                                                                                                                                                       aTCATGCCATACCGCGAAAGGGTTTGCACCATTCGATGGTGTCACCGTAAATGCATGCCGCTTCGCCTTCCCGCCACCAGAATCGCCTGCGATTCAACCCCTTATTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGAtctc  >  1:313607/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                TGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACGTAAATGCATGCCGCTTCGCCTTCCGGCCACCAGAATAGCCTGCGATTCAACCCCTTCTTCGATCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCCGCTGCCTGTTCAATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTT  >  NC_000913/367332‑367796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑