Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,475,493	T→C	F19F (TTT→TTC)	yjgL →	protein YjgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,475,493	0	T	C	100.0%	94.9 / NA	28	F19F (TTT→TTC)	yjgL	protein YjgL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/16);  total (12/16)

GCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCA‑GTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGA  >  NC_000913/4475258‑4475737                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     gcAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCA‑GTAGTGAAGATGCTGAGGGCGTGATGAATTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGaa                                                                                                                                                                                                                                       <  2:385576/251‑1 (MQ=255)   agagggagcaccAGCCCAGGTTCGGAAATCA‑GTAGAGAAGATGCTGCTGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTAAGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGATTAGAGGTTCTTATTTACTCAAGGAGTTCGTCATGAGCCAACTAACAGATTTAAATTATTCTAAACACATTACATTAGCCGACCATTTCaaaaaaaaaatagaagt                                                                                                                                                                                                                                     >  2:198432/11‑244 (MQ=255)           cgcCAGCCCAGGTTCGCAAAGCA‑GTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATa                                                                                                                                                                                                                             <  1:151668/251‑1 (MQ=255)            gcCAGCCCAGGTTAGCAAAGCA‑GTAGTGCAGATGCTGATGTCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTAGGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCCTTAGCAAAATATCAGATTTAAATTATTCTCAAAACATTCCCTTAGCCGACAATTTCAACCACAAAAGCGACGTTTTAAATAc                                                                                                                                                                                                                            >  2:420418/1‑251 (MQ=255)                   gaGGTTCGCAAAGCATGT‑GTTAAGATGCTGATGGCTAGATGACTTGTCGCCGTAAATCACCCAAGGTGTGCGTCTAGTTTGTGAAAAGATCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGGAATGCTTAGACGTTCTTATTCACTCAAGGAGGGCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACTCATTACATTAGACGACAATTTCAAACAAAAAAGTTAAATTTTAAAGACCGGGCg                                                                                                                                                                                                                      <  2:348214/249‑1 (MQ=255)                          gCAAAGCA‑GTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTGAAATACCTGGCTTGTTGgaa                                                                                                                                                                                                              <  1:162009/251‑1 (MQ=255)                                 cagaagtGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTAAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTAAACACATTACAATAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACATGGCGTGTTGGaatgaatg                                                                                                                                                                                                        >  2:39638/5‑251 (MQ=255)                                   gtagtGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGAt                                                                                                                                                                                                      <  1:137497/251‑1 (MQ=255)                                                    ggCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCg                                                                                                                                                                                      <  1:156546/250‑1 (MQ=255)                                                       gTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGATTGTGAAAAGCGCGTTTTTTGATATTTTTTTGTGGGTAAAATTTGTAATGCTTCGACGTTCTTATTCACTCAAGGAGGTCGTCATTAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGAAAATTTCAAACAAAAGAGTGAAGTTTTAAATACCTGGCGTGTTTGAATTAATGATTTAGCCCGTAATGCCggggg                                                                                                                                                                                  >  1:433614/1‑251 (MQ=255)                                                           tgaCTTTTCGCCGTCAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTAACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGc                                                                                                                                                                                >  1:450727/1‑249 (MQ=255)                                                                 ttCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAAc                                                                                                                                                                        >  1:314385/1‑251 (MQ=255)                                                                   cGCCGGAAATAACGCAAGGTTGTCGGCGAGGTTGTGAAAAGAGCGGTTTTTGATATTTTTTGGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTCCAGTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGTGCGTAGGATAACAg                                                                                                                                                                      <  2:224263/251‑1 (MQ=255)                                                                            tAACTAAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAatat                                                                                                                                                             >  1:285435/1‑251 (MQ=255)                                                                                     gTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTcc                                                                                                                                                    <  1:400755/251‑1 (MQ=255)                                                                                                              cGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGCTAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGc                                                                                                                            >  1:172058/1‑250 (MQ=255)                                                                                                                  ttttGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATGCACTCAAGGAGTGCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCTGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGATTTTGGCAAAg                                                                                                                        <  2:62819/250‑1 (MQ=255)                                                                                                                    ttGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGat                                                                                                                      <  2:398656/250‑1 (MQ=255)                                                                                                                                     gTAAAATTTGTAAAGCGTAGACGTTCTTCTTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACAAATAAAATAAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGTATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAAAATAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTAACCTGGGTTAtgt                                                                                                    >  2:354710/1‑251 (MQ=255)                                                                                                                                              gTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTa                                                                                            <  1:325447/250‑1 (MQ=255)                                                                                                                                              gTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAAGATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAg                                                                                           <  2:319056/251‑1 (MQ=255)                                                                                                                                                                                    cATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTc                                                     <  2:192242/251‑1 (MQ=255)                                                                                                                                                                                                atCAGATTTAAATTATTCGCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAAGATGATGGCTCATATTAAGTCCt                                         <  2:117451/251‑1 (MQ=255)                                                                                                                                                                                                                   cAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCCAAAGAt                        <  2:269129/249‑1 (MQ=255)                                                                                                                                                                                                                    aaCACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACATGGCGTATTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGCTAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTCGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCATCATCTTATTATAAAGATACt                     >  1:31347/1‑251 (MQ=255)                                                                                                                                                                                                                          ttacattaGCCGACAATTTCAAACAAAAAATGGAAGTTTTAAATACCTGGCGTGTTAGAATGAATGATTTTGCCCGTATTGCAGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGAATTTTCGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGc               >  2:17174/1‑251 (MQ=255)                                                                                                                                                                                                                             cattaGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAAt            >  1:86789/1‑251 (MQ=255)                                                                                                                                                                                                                                       cAATTTCATACAAAAAAGTGAAGTTTTAAATCCCTGGCGAGTTGGAATGAATTATTTTGCCCGTATTTCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCCTCTGGATCATTTTTAGAGTTTTTGGCAAAGATAGTTTCCCTGGGTTATGTGGATTTTAGCATACGCTCCAACGAAGCGGGTATAAATATGATGGGTCAGATTAATTCCTCATCTTATTCTAAAGATCCGAATCGTAATGAAAATATGa  <  2:82653/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     GCAGTGGGCGCGCCAGCCCAGGTTCGCAAAGCA‑GTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGA  >  NC_000913/4475258‑4475737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑