Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	366,409	C→T	intergenic (‑104/+19)	lacZ ← / ← lacI	beta‑galactosidase/DNA‑binding transcriptional repressor LacI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	366,409	0	C	T	100.0%	128.8 / NA	40	intergenic (‑104/+19)	lacZ/lacI	beta‑galactosidase/DNA‑binding transcriptional repressor LacI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/22);  total (18/22)

GGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG‑CCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG‑CCCCAGCAGGCGAAAATCCTGTTTGATGGTGGT  >  NC_000913/366163‑366652                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       gggCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACAt                                                                                                                                                                                                                                                    >  1:371515/1‑251 (MQ=255)     gATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAAt                                                                                                                                                                                                                                                >  2:157862/1‑251 (MQ=255)           tGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGGGTAAAGCCTGGGGGGCCTAATGAGTGAGCTAACTTACATTAAttgcgtt                                                                                                                                                                                                                                          >  1:104631/1‑251 (MQ=255)                 ccgctTCGCTATTACGCCAGCTGGCGAAAGGGTGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTc                                                                                                                                                                                                                                    <  2:117468/248‑1 (MQ=255)                  ctctTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTc                                                                                                                                                                                                                                    >  1:9831/1‑250 (MQ=255)                   tctTCGCAATTACGCCAGCCGGCGAAAGGAGGATGCGCTGCACGGCGATTCAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAACTTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGATGGAGCTACCTTACATTATTTGGGTGGCGCTCAc                                                                                                                                                                                                                                  >  2:333143/1‑251 (MQ=255)                              aCGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCttt                                                                                                                                                                                                                        <  2:381844/250‑1 (MQ=255)                                           aaaGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAAcc                                                                                                                                                                                                          >  2:461924/1‑251 (MQ=255)                                                          gcAAGGCGATTAAGTGGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCGGT‑CGTGCCAGCTg                                                                                                                                                                                           <  1:69762/250‑1 (MQ=255)                                                            aaGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCAt                                                                                                                                                                                        >  1:157481/1‑251 (MQ=255)                                                                cGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAAt                                                                                                                                                                                    <  1:429747/251‑1 (MQ=255)                                                                 gATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGAGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATg                                                                                                                                                                                   >  1:487507/1‑251 (MQ=255)                                                                  aTTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGTCGTTGTAAAACGACGGCCAGTGAATCCGTCATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTACTTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGAGCCAGCTGCATTAATgc                                                                                                                                                                                  >  2:359213/1‑250 (MQ=255)                                                                    tAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGCCGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAAt                                                                                                                                                                                >  1:140668/1‑251 (MQ=255)                                                                         tGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGc                                                                                                                                                                            <  1:500948/250‑1 (MQ=255)                                                                              aaCGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAAcgc                                                                                                                                                                      >  1:104978/1‑251 (MQ=255)                                                                                cGCCAGGGTTTTCCCAGTCACGACGTTGTAAACCGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCTGCTGCATTAATGAATCGGCCAAcgcgc                                                                                                                                                                    >  1:175199/1‑251 (MQ=255)                                                                                      ggTTTTCCCAGGCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGGTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGgag                                                                                                                                                              <  2:465551/251‑1 (MQ=255)                                                                                                  cACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTTCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGc                                                                                                                                                   <  1:304705/250‑1 (MQ=255)                                                                                                      acgTTGAAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGAATAAAGTGTAAAGCCTGGGGAGCCTCATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCCTTAATGAATCGGCCAACGCGCGGGGAGAAGCGGTTTGCGTAtt                                                                                                                                              >  1:450243/1‑251 (MQ=255)                                                                                                        gTTGTAGAACGACGGCCAGAGAATCCGGAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAATATACGAGACGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGtgtttg                                                                                                                                            >  2:410746/1‑246 (MQ=255)                                                                                                          agTAAAACGCCGGCCAGTGAAGCCGTAAGCATGGGCATAGCTTTTTCCTGTGTGAAATTGTGATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCTCTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGgc                                                                                                                                          <  1:266716/250‑1 (MQ=255)                                                                                                           gTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGgcg                                                                                                                                         <  1:279070/251‑1 (MQ=255)                                                                                                                            gAATCCTTAATCATGGTCAGAGCTTGTTCCTGTGTGAAATTGTTATCCGCTCACAAGTCCCCACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGG‑CGGGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCT‑CCAGGGTGGTttttct                                                                                                                        <  2:488517/250‑1 (MQ=255)                                                                                                                                ccGTAATCATGGGCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACTCAACATACTAGCCGGAAGCATAAAGTGTAAAGCGTGGGGTGCCTAATGAGTTAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CTTTCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG‑CCAGGGTGGTTTTTCTTTTCa                                                                                                                   <  1:54025/251‑1 (MQ=255)                                                                                                                                   tAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAACGCGAGGGGAGAGGCGGTTTTCGTATTGGGCG‑CCCGGGTGGTGTTTTTTTTTAcc                                                                                                                 >  1:472677/1‑250 (MQ=255)                                                                                                                                    aaTCATGGTCATAGCTGTTTCCTGTGAGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG‑CCAGGGTGGTTTTTCTTTTCACCAg                                                                                                               <  1:279381/251‑1 (MQ=255)                                                                                                                                           gTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCCCAATTCCACACAACATACGAGGCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG‑CCAGGGTGGTTTTTCTTTTCACCAGTGAGACg                                                                                                        <  1:333143/251‑1 (MQ=255)                                                                                                                                             cATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG‑CCAGGGTGGTTTTTCTTTTCACCAGTGAGACgg                                                                                                       <  1:461924/250‑1 (MQ=255)                                                                                                                                                    gTTTCCTGAGTGAAATTGTTATCCTCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG‑CCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAg                                                                                                <  1:421389/250‑1 (MQ=255)                                                                                                                                                           gtgtgAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG‑CCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGc                                                                                        <  1:442865/251‑1 (MQ=255)                                                                                                                                                                    tGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG‑CCAGTGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACATCTGATTGCCCTTCAcc                                                                                <  2:307551/250‑1 (MQ=255)                                                                                                                                                                                             aaatcagcgCCGGAAGCATAAAGTGGAAAGCCTGGGGTGCCTAATTAGTGAGCTAACTTACATTAATTGCGTTGCGCACACTGCCCGCTTTCCAGTCGGGAAACGTGT‑CGTGCCAGCTGCTTTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG‑CCCGGGTGGTTTATCTGTTCACCAGTGGTACGGGTAACAGCTGATTTCCCTTCACCGCCTGGCCCTCAGAGGTTTGCAGCaa                                                      <  2:223361/243‑1 (MQ=255)                                                                                                                                                                                                      ccGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG‑CCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCAc                                             <  1:457773/251‑1 (MQ=255)                                                                                                                                                                                                                aaaGTGTAAAGCCTGGGGAGCCTAATGAGTGAGCTAACTTAAATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTGGGGGGCCGGGGGGGTTTTTTTTTTAACC‑GTGAGACGGGGAACAGCTGATTGGCCCTCACCACCCGGCCCCGAGGGAGTTGCAGCaaagggtcccccccgggttgc                                   >  1:400774/1‑232 (MQ=255)                                                                                                                                                                                                                               gggTGCCTAATGAGTGAGCTAACTTACATTAATTGCGGTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAAGTAATCGGCCAACGCGCGGGGAGAGGCGTTTTGCGTATTGGGCG‑CCAGGGTGGTTTTTCTTTGCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGTCCCTGAGAGAGGTGCAGCAAGCGGTCCACGCTGGTTTG‑CCCCAGCAGGCGaaaa                   <  2:467318/251‑1 (MQ=255)                                                                                                                                                                                                                                      tAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTAGGATTTGGCG‑CCCGGGGGGGTTTTCTTTTCACCAGTGATAAGGGGCACAGCTGTGTGCCCTTCACCGCCTCGCCCTGTGAGAATTGCAGCCAACGGGCCCCGCTGGTTTG‑CCCCAGCAGGCGTAAATACTgat            >  1:16123/1‑249 (MQ=255)                                                                                                                                                                                                                                       aaTGAGTGAGCTAACGTACATTAATGGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACC‑GTGCGTGCCAGCTGCTTTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG‑CCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTTCCCTTCACCGCCTGGGCCTTAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG‑CCCCAGCAGGCGAAAATCCTGttt           <  2:40208/251‑1 (MQ=255)                                                                                                                                                                                                                                        aTGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG‑CCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG‑CCCCAGCAGGCGAAAATCCTGTTTg          <  1:293741/251‑1 (MQ=255)                                                                                                                                                                                                                                                gCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTATTGGGCG‑GCAGGGGGGGTTTTCTTTTTACCAGTGAGGCGGGGAACAGATGATTTCCCTTCCCCGCCTGGCCCTCAGGGGGATGCAGCAAACGGGCCACCCCGGTTTGGCCCCA‑CAGGCggcaatccctgtttattgtggg  >  1:385129/1‑230 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       GGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT‑CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCG‑CCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTG‑CCCCAGCAGGCGAAAATCCTGTTTGATGGTGGT  >  NC_000913/366163‑366652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑