Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,707,883	G→A	intergenic (‑178/+733)	dppA ← / ← proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,883	0	G	A	100.0%	86.2 / NA	27	intergenic (‑178/+733)	dppA/proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/13);  total (14/13)

GTCATAGCCACCAGGCTGAGACCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAA  >  NC_000913/3707644‑3708125                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   gTCATAGCCACCAGGCTGAGACCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACGATCTATCAACAGAAtg                                                                                                                                                                                                                                         >  1:657/1‑251 (MQ=255)      aGCCACCAGGCTGAGACCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACGATCTATCAACAGAATGTGaa                                                                                                                                                                                                                                     >  1:339585/1‑250 (MQ=255)                               aGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATCGTGATGTTTGTTGTTTTAACCCTTTGCAGTCGGTTGTCGCTGCCTGCCCTTTTTTGTTTTTTGCCCGGTCGAGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCCTAAAATCACCCTAGTGACCTACGATCTATCACCAGAATGTGACAACGTCAATACCGCCAACCGGGAttt                                                                                                                                                                                                           >  1:488130/1‑251 (MQ=255)                                            ttttCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACGACCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTCCACCAACGGTg                                                                                                                                                                                               >  2:126572/1‑250 (MQ=255)                                                     aaaTACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACGATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCcac                                                                                                                                                                                      >  1:322076/1‑250 (MQ=255)                                                         aCGCATTATTCTTCTTCAATTGTGATGTTTTTTGTTTTAACCCTTGGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACGATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCcacaaaca                                                                                                                                                                                 <  2:132760/251‑1 (MQ=255)                                                             attattCTTCTCCAATTGTGATGTTTGTTTTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTTGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACGATCTATCATCAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGa                                                                                                                                                                             <  1:209769/251‑1 (MQ=255)                                                                 ttCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTACTTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACGATCTATCAACAGAATGTGAAAACGTCAATCCAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAACCAAAGATTa                                                                                                                                                                          >  2:44904/1‑250 (MQ=255)                                                                  tCTGCTCCAATTGTTATGTTTGTTGTTTTAACACTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCATCGTGACCTACGATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTaa                                                                                                                                                                         >  1:158226/1‑250 (MQ=255)                                                                        ccAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACAGCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACGATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACt                                                                                                                                                                  <  1:268126/251‑1 (MQ=255)                                                                                    tttgttGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACGATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTAt                                                                                                                                                      >  1:262122/1‑251 (MQ=255)                                                                                          gttTTAACCCTTTGCAGAGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACGCTCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTgc                                                                                                                                                  >  2:95388/1‑249 (MQ=255)                                                                                                 cccgttGCAGTGGTTTGTCGCTTACGGACCTGTTTTGTTTTTTGCCCCGTCGGGTCAACGTTGTGAGGTGGGGATGCCGTCCTTATTAACATCAGTGTAACAGTTCGGAACCGTCATAAAATTCCCCTCGTGCCCTACGTTCTATCAACAGTATGTGAAAACGTCAATACAGCCAATCGGGATTTACACCAACGGTGAGAATCCACAAAAATAGATTAAAAAAACTTCAAACAGCTACTTGCagaagataa                                                                                                                                         <  2:3527/247‑4 (MQ=255)                                                                                                      tGCAGGGGGGTGTCGCTGCCTGACCTTTTTTTTTTTTGGCCCGGGCGGGTCAACGTTATGAGGTGTGGATGCCGTACTAATTAACCTCAGTGTAACAGTGCGGATCTTCCATAAAATGCCCCTCGTGACCGACTATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCATCGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAAttt                                                                                                                                     <  1:514270/250‑1 (MQ=255)                                                                                                                           gACCTTTTTTGTTTTTTGACCGGTCGGGTCAACGTTATGAGGTGGGGATTCCGTACTAATTAACATCAGTGTAACAGTGCGGCTCCTCCATAAAATGCCCCTAGTGACCTACGAACTATCAACAGATTGTGAAAACGTCAATCCAGCCAAACGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAACAACTTCAAACCGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGAcacg                                                                                                                >  2:454393/1‑247 (MQ=255)                                                                                                                                        ttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACGATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTa                                                                                                   <  2:179553/250‑1 (MQ=255)                                                                                                                                              ccGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACGATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCAcaaacaaa                                                                                                                                                                               <  1:266543/168‑1 (MQ=255)                                                                                                                                              ccGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATACTCCATAAAATGCCCCTCGTGACCTACGATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCAcaaacaaa                                                                                                                                                                               >  2:266543/1‑168 (MQ=255)                                                                                                                                                   cGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACGATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCaa                                                                                        <  1:167517/250‑1 (MQ=255)                                                                                                                                                                            gCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACGATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCAtt                                                              <  1:44904/251‑1 (MQ=255)                                                                                                                                                                            gCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACGATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCAtt                                                              >  1:368816/1‑251 (MQ=255)                                                                                                                                                                               gTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACGATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTaa                                                           <  1:151581/251‑1 (MQ=255)                                                                                                                                                                                                      aGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACGATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGt                                    >  1:8727/1‑251 (MQ=255)                                                                                                                                                                                                          cGGATCCTCCATAAAATGCCCCTCGTGACCTACGATCTATCAACAGAAAGTGAAAACGTCAATACAGCCAACCGGGATTGACACCAACGGTGAGAATCTACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAAGTTGGTCTACTTCAACATTACCTGAACAATCAGCTTAATATTGAGCAACAAGGTGTGATTAAGTTTTATGCAATTAATTAAAAAAATCCTAGGAAAAGGTGGa                                 <  2:92993/250‑1 (MQ=255)                                                                                                                                                                                                                                 cGTGACCTACGATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATg           <  1:364856/249‑1 (MQ=255)                                                                                                                                                                                                                                 cGTGACCTACGATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGa          >  1:196488/1‑250 (MQ=255)                                                                                                                                                                                                                                        aacgaTCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTTTGCTACTCCAACATGACCAGAACAATCATCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGTATATCTGTGCGATTAATCCCATGAACGCTaaa  <  2:232409/247‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   GTCATAGCCACCAGGCTGAGACCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAA  >  NC_000913/3707644‑3708125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑