Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,669,782	A→T	E64V (GAG→GTG)	treF →	cytoplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,669,782	0	A	T	100.0%	125.8 / NA	41	E64V (GAG→GTG)	treF	cytoplasmic trehalase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (27/14);  total (27/14)

ACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAAAATTCAAAACCCTAATCCAGACGAACTGATGATCGAAGTCGATCTCTGCTATGAGCTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGT‑CTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTAAC  >  NC_000913/3669540‑3670019                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               aCCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAAAATTCAAAACCCTAATCCAGACGAACTGATGATCGAAGTCGATCTCTGCTATGAGCTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTc                                                                                                                                                                                                                                        >  1:339652/1‑251 (MQ=255)       aaaGAAAAGCCTATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAAAATTCAAAACCCTAATCCAGACGAACTGATGATCGAAGTCGATCTCTGCTATGAGCTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCgg                                                                                                                                                                                                                                  <  1:17656/251‑1 (MQ=255)             aaGCCGATAATTAGCGACGAATTTCGGAGGTTGTATCCATATGCTCAATCAGAAAATTCAAATCCCTAAGCCAGACGAACTGATGATCGAAGTCGATCTCTGCTATGAGCAGGACCCGTATGAATTAAAACGGTATGAGATGATCTTGGCAGAACCGGACCCCTAGATGATTGAAGTGCTGTCTGCTTCGGATGCTCTGACTCCTGCCGATCGCTATCTCGAACTTTTCGTTCATGTTCATTCTGCGaaaa                                                                                                                                                                                                                            <  2:292467/251‑1 (MQ=255)                 cGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAAAATTCAAAACCCTAATCCAGACGAACTGATGATCGAAGTCGATCTCTGCTATGAGCTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAAtttt                                                                                                                                                                                                                        <  1:212098/251‑1 (MQ=255)                  gATAATTAGAGCGGAATTTCGGAGGTTCGATCCTTATGCACAATAAGAAAATTCAACACCCTAATACAGACGCACTGAAGATCGAAGTCGATCTCTGCTATGAGCTGAACCCGCATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCATACCTCTGATGCGCTGCCGCCAGCCGATCGCTATACCGAACTGTTAGTGCATGTTCAGTCGGCGAAAATATTc                                                                                                                                                                                                                       >  2:307322/1‑251 (MQ=255)                   aTAATTAGCGACTAAGGTCGGAGGGTGGATCCTGATGCTCAATCAGAAAATTCAAAACCCTAAGCCAGTCGAACGGATGATCGAAGTCGATGTCTGCTATGAGCTGGTCCCGTATGAATGAAAACTGGATGAGATGATCGGGGCAGAACCGGAACCCGAGATGATTGAAGGGCGGCCTGCGTCTGATTTGCTGACTCCTGCCGATCGTTATCTCGAACTGTGCGTGCATTTTCAGGCGGTGAAAATTTTcc                                                                                                                                                                                                                      <  2:208294/251‑1 (MQ=255)                                 aTTTCGGAGGGTGGATCCTTATGCTCAATCAGAAAATTCCACACCCTAATCCAGACGAACTGATGATCGCAGTCGCTCTCTGATATGAGCTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACCGTAAAAcc                                                                                                                                                                                                        >  2:341867/1‑251 (MQ=255)                                  tttCGGAGGTTGGATCCTTATGCTCAATCAGAAAATTCAATACCCTAATCCAGACGAACTGATGATCGAAGTCGATCTCTGCTATGAGCTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAACTTTTCCCCGACAGtcacacct                                                                                                                                                                                                       >  2:16824/1‑244 (MQ=255)                                      ggAGGTTGGATCCTTATGCTCAATCAGAAAATTCAAAACCCTAATCCAGACGAACTGATGATCGAAGTCGATCTCTGCTATGAGCTGGACCCGTATGACTTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTcc                                                                                                                                                                                                   >  1:110145/1‑251 (MQ=255)                                              gATCCTTATGCTCAATCAGAAAATTCAAAACCCTAATCCAGACGACCTGATGATCGAAGACGATCTCTGCTATGAGCTGGACCCGTATGAATTAAGACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGACGGGCTGCCTGCCTCTGGTGCGCTGACGCCTGCCGATCGATATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTACCCGACAGTAACCCCTTTCCCGACTgcg                                                                                                                                                                                           >  2:472488/1‑251 (MQ=255)                                                   ttATGCGCAATCAGAAAAGACAAACCCCTAATCCAGCCTAACGGATGACCGAAGTCGATCTCTGCTATGATCGGGACCCGTAGGAATTAAAACTGGAGGAGATGACCGAGGCAGAACCGGAACCCGAGCGGATTGAAGGGCGGCCTGCCGCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCCGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCAcc                                                                                                                                                                                       <  2:58606/250‑1 (MQ=255)                                                                    aTTCAAATCCCTAATCCAGACGAACTGATGATCGAAGTCGATCTCTGCTAGGAGCTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGa                                                                                                                                                                     <  1:43554/251‑1 (MQ=255)                                                                       cAAAACCCTAATCCAGACGAACTGATGATCGAAGTCGATCTCTGCTATGAGCTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGatat                                                                                                                                                                  >  1:346725/1‑251 (MQ=255)                                                                           aCCCTAATCCAGACGAACTGATGATCGAAGTCGATCTCTGCTATGAGCTGGACCCGGATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTTCGCACCTAAAATGGACCCGCTGGTTATCtt                                                                                                                                                               >  2:379837/1‑250 (MQ=255)                                                                            cccTAATCCAGACGAACTGATGATCGAAGTCGATCTCTGCTATGAACTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACAGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGAATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCACGACTGCGCCCATAAAATGGACCCGCTGGATATCTTa                                                                                                                                                              >  2:306099/1‑250 (MQ=255)                                                                                     aGACGAACTGATGATCGAAGTCGATCTCTGCTATGAGCTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAGTCCGCTAcc                                                                                                                                                    >  2:19435/1‑251 (MQ=255)                                                                                           aCTGATGATCAAAGTCGATCTCTGCTAGGAGCTGAACCCGTATGAATTAAAACTGGATGAGATGACCGAGGCAGACCCGGAACCCGAGAGGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTCCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAg                                                                                                                                              <  2:210786/251‑1 (MQ=255)                                                                                                    cGAAGTCGATCTCTGCTATGAGCTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTc                                                                                                                                     >  2:477825/1‑251 (MQ=255)                                                                                                      aaggcGATCTCTGCTTCGTGCTGGCCCCGTATGATTTAAATCTGTATGATATGATCTAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTCCCGGCCTCTGATGCGCTGACGCCTTCCGATCGCTATCTCGATCTTTTCGTGCATGTCCAGTCGGCTAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTTCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGGGCGCCGTCAt                                                                                                                                   <  1:381660/247‑1 (MQ=255)                                                                                                        gTCGATATCTGCTATGAGCTGGTCACGTATGAATTCAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGATCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGACCTGTTCGTGCATGTTCAGTCGGCTAAAATTTTCCCCGACAGTAATACATTTCCCGACGGCGCACCTCAAATGGACCCGCTGGATATCTTAATCCGCTCCCGTAACGTGCGCCGTCATCg                                                                                                                                 >  2:375901/1‑251 (MQ=255)                                                                                                         tCGATCTCAGCTAAGAGCTAGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGAGCTGCCTGCCTATGATGCGCTGACGCCTGCCGATCGCTATCTCGCACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGCCAGTAAAACCTTTACAGACTGCGCACCTAAAATTGACCCGCTGGATATCATAATCCGCGACCGTAAAGTGCGCCGTCATCgc                                                                                                                                >  2:114079/1‑250 (MQ=255)                                                                                                                  gCTAGGAGCTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGa                                                                                                                        <  1:12212/250‑1 (MQ=255)                                                                                                                             gACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATAGTGATTTTGACTTGCGCAAGtt                                                                                                            >  1:59761/1‑251 (MQ=255)                                                                                                                                  gTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTttgttg                                                                                                       <  1:477825/251‑1 (MQ=255)                                                                                                                                       aaTTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGCCTTGCGCAAGTTTGTTGAAAAc                                                                                                  >  1:200169/1‑251 (MQ=255)                                                                                                                                             aaCTGGATGAGATGATCGCGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATAGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCCCTTc                                                                                            >  2:78224/1‑251 (MQ=255)                                                                                                                                                 ggATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGc                                                                                        >  1:397065/1‑251 (MQ=255)                                                                                                                                                                ggCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGT‑CTACt                                                                        >  1:291671/1‑251 (MQ=255)                                                                                                                                                                 gCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGT‑CTACTc                                                                       >  1:446537/1‑251 (MQ=255)                                                                                                                                                                  cAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGACAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGACGGAGGT‑CTACTcc                                                                      >  1:34633/1‑251 (MQ=255)                                                                                                                                                                   aGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCACGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGT‑CTACTcc                                                                      >  1:81164/1‑250 (MQ=255)                                                                                                                                                                     aaCCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTATGATGCTCTGACGCCTGCCGAAAGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATAACTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGCCTTGCGCCAGTTTGTTGAAAACAACTTCTGGCTGCCGACGGT‑CTACTCCAGc                                                                   >  2:389427/1‑251 (MQ=255)                                                                                                                                                                      aCCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTGCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGTATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGT‑CTACTCCAGCg                                                                  <  1:53373/251‑1 (MQ=255)                                                                                                                                                                         ggAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGT‑CTACTCCAGCGAgt                                                               >  1:120478/1‑251 (MQ=255)                                                                                                                                                                                    tgaatGAAGAGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATAGCTATGGCGAACTGTTCGTGCATGTTCTGTCGGAGAAAATTTTCCCCGCCAGTAACACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTAAGCGTGATTTTGACTTGCGCACGTTAGTTGAAAACCACTTCTACCTGCCGGTGGT‑CTACTCCAGCGCgtatgtctctgac                                                    >  2:246015/5‑244 (MQ=255)                                                                                                                                                                                         gAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAACTTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCCCCTAAAATGGACCCGCTGGATATCTTAATCCGCTCCCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCCAGTTTGTTGAAAACCACTTCTGGCTGCCGG‑GGTACTACTCCATCGAGTATGTATCGGACCCGc                                                >  1:88842/1‑250 (MQ=255)                                                                                                                                                                                                           gATGCGCTGCCGCGTGCCGATCGTTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGGAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGT‑CTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCa                             <  2:224772/251‑1 (MQ=255)                                                                                                                                                                                                            aTGCGCTGCCGCCGGCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGGCGGCGAAAATTTCCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGT‑CTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCat                            <  2:129827/251‑1 (MQ=255)                                                                                                                                                                                                                          gCCGATCGCTATCTCGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGT‑CTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCtgtg              >  1:503408/1‑251 (MQ=255)                                                                                                                                                                                                                                  cTATCTCGAACTGTTCGTGCATGTTAAGTCGGCGAACATTTTCCCCGACAGAACAACCTTTCCCGACAGCGGACCTAAAATGGCCCCGCTGGATATCTTACTCCGCTACCGTAAAGAGCGCCGTCCTCGTGATTTTAACTTGCGCAAGATTGTTGAAAACAACTTCTGGCAGCCGGAGGT‑CTACTCCAGCGAGTATGTATCAGACCCGCAAAATTCCCTGAAAGAGCATATCAACCAGCTGTGGCCGCTGc      >  2:496329/1‑251 (MQ=255)                                                                                                                                                                                                                                        cGAACTGTTCGTGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAATCCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGT‑CTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTAAc  <  1:510002/249‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               ACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAAAATTCAAAACCCTAATCCAGACGAACTGATGATCGAAGTCGATCTCTGCTATGAGCTGGACCCGTATGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCTGCCTCTGATGCGCTGACGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGCGAAAATTTTCCCCGACAGTAAAACCTTTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGT‑CTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTAAC  >  NC_000913/3669540‑3670019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑