Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,011	T→A	L81H (CTT→CAT)	mprA →	DNA‑binding transcriptional regulator MprA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,011	0	T	A	100.0%	140.4 / NA	40	L81H (CTT→CAT)	mprA	DNA‑binding transcriptional regulator MprA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (17/23);  total (17/23)

TTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGAC  >  NC_000913/2810783‑2811258                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        taaCGCCCATTGCACACATGCTAAGATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCACGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGATCATGGATCATCCCGTACCAacgcg                                                                                                                                                                                                                                   >  2:411082/3‑251 (MQ=255)   aagCCCATGGAACAAATGAGAAAATTTCGCGCCAGCCGCCACGAAGATTGTCCGTATCAGGAGATCCTTCTGACTCGTCTTTGCAGGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAGTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGATCATGGATCATCCCGTACCAacgcgac                                                                                                                                                                                                                                 <  2:117949/249‑1 (MQ=255)      cccATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAacgcgacgcg                                                                                                                                                                                                                              >  1:126601/1‑251 (MQ=255)                                cgcCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAAcgcg                                                                                                                                                                                                    >  1:356674/1‑251 (MQ=255)                                  ccAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCGTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTAGTGCCGATGAACTGGAAAAACGCGGt                                                                                                                                                                                                  <  2:468769/251‑1 (MQ=255)                                   cAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGtt                                                                                                                                                                                                 >  2:107389/1‑251 (MQ=255)                                   cAGCCGCCACGAAGATTTTCATTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGtg                                                                                                                                                                                                 >  2:115632/1‑250 (MQ=255)                                        gccACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTCTTGCCGATGAACTGGAAAAACGAGGTTGGATc                                                                                                                                                                                            >  1:90691/1‑251 (MQ=255)                                                    ttCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATCCAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGATCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAAAGCGGTTGGATCGAACGTCGTGa                                                                                                                                                                                 >  1:322096/1‑250 (MQ=255)                                                     tCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGaa                                                                                                                                                                                <  1:132944/250‑1 (MQ=255)                                                      ccTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGAAGTTGTTTATGGCGTTGATTACGCTGGAGTATCAGGAAAACCACAGTATTCAGCCTTCTGACTTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGaa                                                                                                                                                                                >  1:506419/1‑249 (MQ=255)                                                                   tCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGACTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATcgc                                                                                                                                                                 >  1:486173/1‑251 (MQ=255)                                                                              cGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGAGAACGATCGCCGctgcctg                                                                                                                                                        <  1:96555/249‑1 (MQ=255)                                                                                   ttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATc                                                                                                                                                    <  2:125265/248‑1 (MQ=255)                                                                                      catgtacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTACCGAGACGGTGTTTATTGCGGTGATTACGCTGGAGTCTCAGGAAAACCACTGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCTACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTTCATCTGCAAt                                                                                                                                              <  2:246019/251‑1 (MQ=255)                                                                                      catgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAAt                                                                                                                                              >  1:433907/1‑251 (MQ=255)                                                                                       atgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCTTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTTCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGTATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAAtt                                                                                                                                             <  2:298014/251‑1 (MQ=255)                                                                                       atgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAAtt                                                                                                                                             >  1:305604/1‑251 (MQ=255)                                                                                         gcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTa                                                                                                                                            >  1:508131/1‑250 (MQ=255)                                                                                          cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAAc                                                                                                                                          >  1:257390/1‑251 (MQ=255)                                                                                             atgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCt                                                                                                                                                   <  2:365732/239‑1 (MQ=255)                                                                                             atgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCt                                                                                                                                                   >  1:365732/1‑239 (MQ=255)                                                                                               gcaAAGCAAGCTGCTGGAGAACCGCAATAAAATTCTGAAGGCGTC‑GGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCTTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTTCAATTAACGGaa                                                                                                                                      <  2:41534/250‑1 (MQ=255)                                                                                                          tgctgGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGCGTCTCAGGAAAACCACCGTATTCAGCCTTCTGAATTAAGCAGTGCTCATGGATAATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGCCACg                                                                                                                            >  1:298797/1‑249 (MQ=255)                                                                                                               gagaACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGttttt                                                                                                                     >  1:374689/1‑251 (MQ=255)                                                                                                                           aaactgatGAAGGC‑TCAGTGGATTAACTAGACGTTGTTTATGGCGGTGATTACGCTGGAGTCACAGGAAAACCACAGAATGCAGCCTTCTGAAGTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGAAGCGTATTGCCGAGGAACGGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGAGCGCCGCTGCCTGCATCTGCAATTAGCGGAAAAAGGTCCCGAATTTGTGCGCGAGGttt                                                                                                          <  2:473959/243‑1 (MQ=255)                                                                                                                                            aGGGGATTAACGAGTCGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATGCAGCCTTCTGAATTAATTTGTTCTCATGCATCATCCCGGACCAACGCGACGCGTATTGCCGATGAACTTGAAAAACGCTGTTGGATCGAACGTCGTGAAAGCGATAACGATCTCCGCTGCTTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAAc                                                                                         <  1:84401/251‑1 (MQ=255)                                                                                                                                             ggggATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAAct                                                                                        <  1:446157/251‑1 (MQ=255)                                                                                                                                                                     ggCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCcgcg                                                                 <  1:115632/250‑1 (MQ=255)                                                                                                                                                                        gTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCa                                                             <  1:107389/251‑1 (MQ=255)                                                                                                                                                                          tGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTCTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAg                                                            <  2:305604/250‑1 (MQ=255)                                                                                                                                                                               aCGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCacaac                                                      <  1:445421/251‑1 (MQ=255)                                                                                                                                                                                           cAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATc                                           <  2:257390/250‑1 (MQ=255)                                                                                                                                                                                                aaaCCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCg                                     <  1:36607/251‑1 (MQ=255)                                                                                                                                                                                                     acaggaataagacTTCTGAATTAAATGGTGCTAATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCaa                                <  2:174329/239‑1 (MQ=255)                                                                                                                                                                                                           ttCAGCCTTCTGAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCAcc                          >  1:114750/1‑251 (MQ=255)                                                                                                                                                                                                            tCAGCCTACTGAATTAAGTTGTTCTCATGGAGCAACCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCCCGAGTTTTTGCGCGAGGTTTTACCACCTCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGATCAAATCAca                          <  2:164721/250‑2 (MQ=255)                                                                                                                                                                                                                      gAATTAAGTTGTGCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGc                <  1:263013/250‑1 (MQ=255)                                                                                                                                                                                                                                gtgCTCATGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGGTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTTCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGTTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTACCGtctc     <  2:374689/251‑1 (MQ=255)                                                                                                                                                                                                                                gtgCTCATGGATCATCCCGGACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCCCCCGCAAATTGCTCTCCCGtctc     <  2:201440/251‑1 (MQ=255)                                                                                                                                                                                                                                     ctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGAc  <  2:471785/249‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        TTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGC‑TCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTGAATTAAGTTGTGCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGAC  >  NC_000913/2810783‑2811258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑