Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,173,448	C→T	intergenic (‑575/+834)	gatD ← / ← gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,173,448	0	C	T	100.0%	71.2 / NA	28	intergenic (‑575/+834)	gatD/gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/17);  total (11/17)

GAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCG‑ATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGC  >  NC_000913/2173201‑2173693                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      gagaTTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCG‑ATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAgcttgctt                                                                                                                                                                                                                                                  >  1:391183/1‑251 (MQ=255)           ggCGGAACAGATTCCCACGATGCACGGCGACCTCCATCGCCACGAAGAATCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCATTTAAAATGGTGAGTGGGATAAAAAGCAGGCTTGCCG‑ATACCACC‑‑TCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGc                                                                                                                                                                                                                                        <  1:85212/250‑1 (MQ=255)                         ccACGATGCACGGCGACCGCCATCGCCACGCAGAAGCCGATGGTGGCAAGATCGCCCAACCGCAGCACATGATTACCCGGCACCCAATCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCG‑ATCCCACCC‑‑CCGTATGTCCAAGCAGCATCGCCGGATCAAGGCCCATCAGGAACTCCTGACCGCCGAACTTCGCCTGTGACCCTCTTCGAGCTTGCTTAGCGATGGGCGTTAACccatccct                                                                                                                                                                                                                          >  2:154823/1‑249 (MQ=255)                           aCGATGCCCGGCGCCCGCCATCGCCACGAAGAAGCCGATGGGGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGCCACACAAACAGCAATTAAAAGGGGGAGTGAGATAAAAATCAGGCTTGCCG‑AT‑CC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCatga                                                                                                                                                                                                                        <  1:495643/251‑1 (MQ=255)                                                  ccaagaagaaGCCGATGGTGGCAATATCGCCAATCGGCAGCACCTGATTACCCGGCACACAATCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCG‑ATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAtt                                                                                                                                                                                                 <  1:65048/247‑1 (MQ=255)                                                           ggCCGATGGTGGCAAGATCGCCAAACGGCGGCACCTGATTACCCGGCACACAACCAGCAATTAAAAGGGTGAGTGGGAAAAAAATCAGGCTTCCCG‑ATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATc                                                                                                                                                                                        <  2:158948/250‑1 (MQ=255)                                                               gATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCG‑ATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTg                                                                                                                                                                                    >  1:441839/1‑251 (MQ=255)                                                                    gggCAAGATCGCCAAACGGCAGCTCCTGATTACCCGGCACACAAACAGCAATTAAAATGGGGAGTGGGATAAAAATCAGGCTTGCCG‑ATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTGAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTgccgcc                                                                                                                                                                               <  1:321690/250‑1 (MQ=255)                                                                          gATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCG‑ATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTATCAGCATCACTTCCGCCGttttt                                                                                                                                                                         >  1:92212/1‑251 (MQ=255)                                                                              gccaaactggagcaactgcatACCCGGCCCACAAACAGCAATTAAAATGGTGCGTGGGATAAAAATCAGGCTTGCCG‑ATACCACC‑‑GCCGTATGTCCCCGCAGAAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAAATTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGTCGTTCAACCTTCCATGCTGGGTTTAATCACCCATGGCATTAGAAGCATCACTGCAGCCGTTTTTAccc                                                                                                                                                                     >  2:37779/21‑250 (MQ=255)                                                                                cAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCG‑ATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                                                                                                                                   >  1:146666/1‑251 (MQ=255)                                                                                           ccataattccccgccaaacaACCAGAAATAAAATTGGGGAGTGAGATAAAAACCAGCTTTCCCG‑AT‑CC‑CCCCGCCGTATGTCCCATCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGAATTAGCAGCATCACTGCCGCCGTTTTTTCCGCCAGCTGCAAt                                                                                                                                                          <  2:22812/232‑1 (MQ=255)                                                                                                ataccccGGCACAAAAGCAGCAATAAAAATGGGGAGGGTGAAAAAATTCAGGCT‑GCCGGAT‑CC‑CGCCGCCGTATGCCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTGCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCtt                                                                                                                                                   <  1:283541/247‑1 (MQ=255)                                                                                                              aaaCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCG‑ATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCACGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGATTAGCGATGGGCGTTAAACCATCCTTGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAcccc                                                                                                                                     >  2:165239/1‑251 (MQ=255)                                                                                                               aacaacAATAAAAATGGTGAGTGGGAAAAAAACCAGGCTTGCCA‑AT‑CC‑CCCCGCCGTAGGGCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCCCTGCCGCCGTTTTGACCGCCAGCTTCAATACACCTTTGACATCGTAACCcg                                                                                                                                    <  2:494356/251‑1 (MQ=255)                                                                                                                    cAATTAAAATGGTGATTGGGATAAAAATCAGGCTTGCCG‑ATTCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAgg                                                                                                                               <  1:304598/251‑1 (MQ=255)                                                                                                                                            aaaaaacaaggctcacagaaccccccGCCGTAGTTCCCAGCAGAAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCAGCCATGATGGGTTTAAGCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCGTTGACATCGTAACCCGCTAGGAGGCCGATAATCAGCCCCATc                                                                                                          <  2:56873/229‑1 (MQ=255)                                                                                                                                                     gctttccagatgccaccgccgTATGCCCCAGCAGAAACCCCGGACCAAGGCCAATCAGGATCTCCTGCCCCCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGTTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCGTTGACATCGTATCCCGCGAGGATGCCGATAATCAGCCCCATCACAAAAccc                                                                                                 <  2:58593/236‑1 (MQ=255)                                                                                                                                                         cG‑ATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGACAATCAGGAACTCCTGAACGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGGGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATAACAAAACCCACgg                                                                                             >  1:103308/1‑248 (MQ=255)                                                                                                                                                             gataccaccgccgTATGTCCCAGCAGCAAAGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTCAACAATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGAc                                                                                         >  1:29167/8‑251 (MQ=255)                                                                                                                                                                       ccttatttcccatctgcatcGCCGGATCAAGACCTATCAGGACCTCCGGACCGCGCAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCTATGGGCGTTAAACCATCCAGGATGGGTTTAATCCCCCGTGGCATTAGCAGCATCACTGCCGCCGGTTTTACCGCCAGCTGCAATACACCTTTGTCATCGTAACCCTCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCa                                                                               <  1:26302/232‑1 (MQ=255)                                                                                                                                                                                ccaccagcatcccctgacccaGGCCAAACAGGAACTCCTGACCGACGAACGTCGCCTGTAATCTACTACGATCTTGCGTAGCGATGGTCGTTTAACCATCCATGATGTGTTTAATCACCCGTGTCATGTGCAGCATCTCTGCCGCCGTTGTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGACGCCGATAATCAGCCCCATCACAAAACCCCCGGTGACATGCTCGCCAAATGGACCa                                                                      <  1:66120/231‑1 (MQ=255)                                                                                                                                                                                 cagcacCAACGCCTGATCAAGGCCAATCAGGAACTCCGTACCGTCGAACTTCGCCTGTAAAAGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAACACCCGGGGCATTAGCAGCATCACGGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATTCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGTCTCGCCAAATGGACCaa                                                                     <  2:391183/251‑1 (MQ=255)                                                                                                                                                                                       caACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTgc                                                               >  2:340342/1‑251 (MQ=255)                                                                                                                                                                                        aaCGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTgcg                                                              >  1:381135/1‑251 (MQ=255)                                                                                                                                                                                          cGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCt                                                            <  1:415500/251‑1 (MQ=255)                                                                                                                                                                                           gCCGGCTCAAAGCAAATCAGGAACTCCTAACCGCCGACATTCGCCTGTAACCGACTACGAGCTTGCTTAGCGACGGGAGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAACATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACACCGTAACCCGCGAGACTGCCGATAATCAGCCCAATCACAAAACCCACGGTGACAGGCTCGCCAAATGCACCAAATTTgcgatt                                                           >  1:149105/1‑248 (MQ=255)                                                                                                                                                                                                                           gccgAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTaa                           <  1:15644/251‑1 (MQ=255)                                                                                                                                                                                                                                                    cGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGc  <  1:476614/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      GAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCG‑ATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGC  >  NC_000913/2173201‑2173693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑