Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,042,171	G→A	intergenic (+146/‑197)	zinT → / → yodB	metal‑binding protein ZinT/putative cytochrome b561 YodB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,042,171	0	G	A	100.0%	53.6 / NA	18	intergenic (+146/‑197)	zinT/yodB	metal‑binding protein ZinT/putative cytochrome b561 YodB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/7);  total (11/7)

AATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTGAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTCAAATTTATTTACATTGGATAACG  >  NC_000913/2041934‑2042412                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  aaTCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGTTCTATGAAATGATGTCTCATTGATTATTCGCATGATAACGCCTCGATGTCGCTTTAGTAAGTTATCATAACTGCCACTGTTCCATCCACAAACGCCACTTAACGCAAGCTATCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATaa                                                                                                                                                                                                                                      <  1:206703/251‑1 (MQ=255)       tattaAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTATCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGAtt                                                                                                                                                                                                                                >  2:216478/1‑251 (MQ=255)                            ggCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAAc                                                                                                                                                                                                            >  1:214117/1‑250 (MQ=255)                                aaCGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATCTTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACag                                                                                                                                                                                                          >  1:335634/1‑248 (MQ=255)                                                                         aGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTa                                                                                                                                                              <  1:379622/251‑1 (MQ=255)                                                                                  tgTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGc                                                                                                                                                      >  1:44376/1‑250 (MQ=255)                                                                                       cATTGAGTATTCTCATGAAAACGCCGCGATGCCGCTTTAGTAATTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACt                                                                                                                                                   <  1:204567/248‑1 (MQ=255)                                                                                        aTTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCa                                                                                                                                               <  1:163121/251‑1 (MQ=255)                                                                                          tGAGTATTCTCATGCTAACGCCTCGATGCCGCTTTAATAAGTAATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCACGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTACCCCGCATTAAGTTAACCAATACCGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGAGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAAt                                                                                                                                             >  2:53870/1‑251 (MQ=255)                                                                                          tGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAAt                                                                                                                                             >  1:484378/1‑251 (MQ=255)                                                                                                            cgcttagtagtcggtttAGTAAGTAATCATAACTGCCACTGGTCCATCAACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTACGACGCGTCAGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATCAAGGATTCCATACACAATACGGCCTAAAGAGAAAGATACCTGTGCTCACACCATTGCTTATATTGGCTGGATACAATATGCACTATACATTTTTTAACTAACAAatt                                                                                                                            >  2:233202/15‑250 (MQ=255)                                                                                                             gCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAattat                                                                                                                          <  1:427162/251‑1 (MQ=255)                                                                                                                                aaGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCaa                                                                                                                                                                                                                                         <  1:92267/121‑1 (MQ=255)                                                                                                                                aaGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCaa                                                                                                                                                                                                                                         >  2:92267/1‑121 (MQ=255)                                                                                                                                                           acacaAACGCCACTGAACGCAAGCTAGCTACAGACAAGCTCATCAATCTGACGATAATGTATATCAATAAGCTAACCCGCATTAAGTTACCCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAACTACCTGTGCTCACGCCATTGATTATATTGCCTGGTTAAAAAATGCACTATCAATTTTTTAAATAACAAATTATTAACACAACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCt                                                                            >  2:273644/2‑251 (MQ=255)                                                                                                                                                                             gCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAg                                                          <  1:108908/251‑1 (MQ=255)                                                                                                                                                                                                gCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCg                                       >  1:92252/1‑251 (MQ=255)                                                                                                                                                                                                                                     aCCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTCAAATTTATTTACATTGGATAACg  >  2:281273/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  AATCTTTATTAAATGAAATGGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTGAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTCAAATTTATTTACATTGGATAACG  >  NC_000913/2041934‑2042412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑