Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,829,887	G→A	A293A (GCC→GCT)	astA ←	arginine N‑succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,829,887	0	G	A	97.5%	114.2 / NA	40	A293A (GCC→GCT)	astA	arginine N‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (19/20);  minor base T (1/0);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

CGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGAT  >  NC_000913/1829659‑1830132                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      cGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGaa                                                                                                                                                                                                                                   <  1:121073/249‑1 (MQ=255)     cGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTTAAATCAAACGCTCGGTTGCCGGATCGGGTCGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTTGCGCCCAGGCAGGCTGGGGAAACGcc                                                                                                                                                                                                                             >  2:87669/1‑251 (MQ=255)     cGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGcc                                                                                                                                                                                                                             <  1:379873/251‑1 (MQ=255)       aTACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCCCCAGCCCCACGCGGAAATGGTGATAATTTTCATTAGCGACCCGGCAGGATGGGAAATCGCCCt                                                                                                                                                                                                                           >  1:289342/1‑251 (MQ=255)         aCCGGATTACGTTTCACACGCGATGCGCCCTGGCCCGTTATCCCGTCACCGTTAATCCATAAAGTCATGCTTGTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGGTAAAATCAAACGCTCGGTGGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGTCTGGGAAATCGCCCTgc                                                                                                                                                                                                                         <  2:264540/251‑1 (MQ=255)                 ccGCTTCACCCGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCGCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTg                                                                                                                                                                                                                 <  2:381828/250‑1 (MQ=255)                    cTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATACCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAACAGCTCGGTTGCCCGGTCGGTACGCACCAGCCCCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCCGGCTGGGCAAACGCCCCGCGCCGGCTGccc                                                                                                                                                                                                              >  2:424543/1‑251 (MQ=255)                                     ccTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTccacca                                                                                                                                                                                             <  1:371404/251‑1 (MQ=255)                                                            ttAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGGTGCCGGATCGGTACGCCCCAGCCCCACGCGGCAATGGTGATAATTTTCTTTAGCGACCAGGCAGCCCGGGAAAGCGCCCTGCGCCGGCTGCCCTTCCGCCACCTCCACCAGCCGACTTTTACGGATGgcgcgc                                                                                                                                                                      >  2:216634/1‑251 (MQ=255)                                                                       aGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGAt                                                                                                                                                           >  1:111541/1‑251 (MQ=255)                                                                       aGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGAt                                                                                                                                                           >  1:393840/1‑251 (MQ=255)                                                                         tCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCCTTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCCCTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGt                                                                                                                                                         >  1:1146/1‑251 (MQ=255)                                                                                       cctcGGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGt                                                                                                                                            <  2:397234/250‑1 (MQ=255)                                                                                        ctctGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATTAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCCCCAGCCGACTTTTACGGGTGGCGCGCCCGCGGGCGATGTCACACTCAAGCGTCg                                                                                                                                          >  1:99508/1‑251 (MQ=255)                                                                                            gcgcACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCgg                                                                                                                                         <  2:139330/248‑1 (MQ=255)                                                                                             cgcACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTGTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCa                                                                                                                                     <  1:191478/251‑1 (MQ=255)                                                                                                    gcgcACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCTGTTGTGCGGCGGTTAAAATCAAGCGCTCGGTTGCCGGATCGGTACGCACCGTCACCACGCGGAAATGGTGATAATTTTCAGTAGCGAGCAGGCAGGCTGGGAATGCTCGCTTCGCAGGTTGCGCATCTGCTTCTGTTACCAGCCGAGTCTGACGGATGCTGCGCGCGGGGGGGATGTCACACTCTAGCGTCGGCCCGCCGTCaa                                                                                                                              <  2:266443/251‑1 (MQ=255)                                                                                                            gACGAACGCGATCCCCGGGGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAACAGCTCCGTTGCCGGATCGGTACGCACCAGCACCACGCGGGAATGGTGATAATTTTTATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAAGCTGCCCTTCTGCCACTTCCCCCAGCCCACTTTTAAGGATGGCGCGCCCGAGGTCGATGTCACCCTCACGCGTCGGccccccctccacgctgtcg                                                                                                                      >  1:85281/1‑235 (MQ=255)                                                                                                                 aCGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGATAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGGCCCACGCGGAAATGGTGGTAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGGCCTTCTGCCACTTCCACCAGCCGACTTTTACGGAAGGGGCGCACGCGGGCGATGTCGCACTCAAGCGGCGGCCCACCggccaaagaggcggttt                                                                                                                  >  2:150098/1‑234 (MQ=255)                                                                                                                                   cATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGGTCGGTTGCCGGATCGGTACGGACCAGAACCACGCGGGAATGGGGATAATTTTTATTAGCGAACAGGCAGGATGGGAAAATGCCCTGCGCAGGCTGTCCTTCTGCCACTTCCACCCGCAGACTTTTACGGATGGCGCGCACGCGGTTGATGTCACCCTCCAGCGGCGGCCCAACGTCAAAGATGACAATTAAATTAAGGTAGCGaaaaac                                                                                               >  1:496775/1‑249 (MQ=255)                                                                                                                                    aTTTTAGGGCATCCAGTTGTGCGGCGGTTAAAAGCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTATCGAAAACct                                                                                              <  2:204105/251‑1 (MQ=255)                                                                                                                                     tgtGAGGGCTTCCCGTGGTGCGGCTGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCCCCAGCCCCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGAACGCGGTCGATGTCACACTCAAGCGTCGTCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACct                                                                                              <  2:326339/248‑1 (MQ=255)                                                                                                                                       tGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACcttc                                                                                            <  2:169577/250‑1 (MQ=255)                                                                                                                                        taGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCATCACCACGCGGACATGGTGAGAATTTTCATTAGCGACCAGGCAGGCAGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCTATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACcttct                                                                                           <  2:396032/249‑1 (MQ=255)                                                                                                                                            gCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCCCCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTtctc                                                                                      <  2:384934/251‑1 (MQ=255)                                                                                                                                                gcaggtgggcggcgGTTAAATTCAAACGCTCGGTTGCCGGATCGGTTCGCACCAGCACCACGCGGAAATGGTGATAATTTGCATTAGCGACCAGGCAGGCTGGGAAAGCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAATCTTCTTTCTCCa                                                                                    <  2:65220/241‑1 (MQ=255)                                                                                                                                                                        aaCGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCt                                                                                                                                                                                                          <  2:441656/107‑1 (MQ=255)                                                                                                                                                                        aaCGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCt                                                                                                                                                                                                          >  1:441656/1‑107 (MQ=255)                                                                                                                                                                          cgatCGGTTGCCGGATCGGAACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTAAGGATGGCGCACACGCGGTCGGTGTCACACTCAAGCCTCGGCCCACCGTCGAAGATGGAGATATAGGTACGGAAGCGGAAACCTTCTTTCTCCAGCAACGCACTGTCGGGCGCGGTTTGctg                                                        >  2:405545/4‑249 (MQ=255)                                                                                                                                                                                 ttGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCCACTTTTACGGATGGCCCGCACGCGTTCGATGTCACACTCAAGCGTCGGCCCACCGCCAAAGATGTCGATATAGTCACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGTTTTCCGGATGGAcc                                                 >  1:227567/1‑251 (MQ=255)                                                                                                                                                                                       gATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCg                                           <  1:216634/251‑1 (MQ=255)                                                                                                                                                                                                       caccacGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCtt                           >  1:335772/1‑251 (MQ=255)                                                                                                                                                                                                        accacGCGGAAATGGAGATAATTTTCATTAGCGACCAGGCAGGCTGGGCAATCGCCCTGCGCAGGCTGCCCTTATGCCACTTCCACCAGCCGTCTTTTACGGTTGACGCGCACGCGGTCGATGTCACACTCTAGCGTCGTCCCACCGTCTAAGATGTTGATATAGTTACGGTAGCGCAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCttt                          >  1:31359/1‑250 (MQ=255)                                                                                                                                                                                                         ccacGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGACGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCATCGGCCCACCGTGAAAGATGTCGATATAGTTACGGTAGAGAAAACCTTCTTTCTCAAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTcc                         >  1:36011/1‑251 (MQ=255)                                                                                                                                                                                                                gAAATGGAGATAATATACATTAGCGACAAGGCAGGCTGCGAAATAGACATGAGCAGGCTGCCCTTCTGCCACTTACACCAGCCGACTTTTACGGATGGCGAGCACGCGGTCGATGTCACACTCAAGAGTCAGCCCACCGTCCCAGATGACGATATAGTTACGGTAGCGAAAACCTTCTTTATCAAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTTCCTGACCGAAGACAGCCCGGGCGTCCTGGGAt                   >  2:62907/1‑250 (MQ=255)                                                                                                                                                                                                                   aTGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATaaaa               <  1:429339/251‑1 (MQ=255)                                                                                                                                                                                                                   aTGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATaaa                >  1:379916/1‑250 (MQ=255)                                                                                                                                                                                                                      gTGATACTTTTCATCAGCGACCCGACAAGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTATGACACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGGCCAAAATGTCGATATAGTTACGGTAGCGAAACCCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGGTGACCTCATGGGCTTCCTgggctacacagt            >  2:438991/1‑242 (MQ=255)                                                                                                                                                                                                                        gATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTgg          <  1:514844/251‑1 (MQ=255)                                                                                                                                                                                                                                 cATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGAt  <  1:275483/250‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      CGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGAT  >  NC_000913/1829659‑1830132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑