Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,652,3310TC93.3% 45.1 / ‑5.4 15intergenic (+2346/+397)ydfD/ynfPlysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (2/12);  total (3/12)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTC‑ACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGC  >  NC_000913/1652100‑1652557
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                  
gTTTATACTGGAGTTCGCCCTGTTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGcaga                                                                                                                                                                                                                   <  1:45825/249‑1 (MQ=255)
                  ccTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAGCGAGACTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCAc                                                                                                                                                                                               <  1:239264/251‑1 (MQ=255)
                        gAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGt                                                                                                                                                                                         <  2:31735/251‑1 (MQ=255)
                                                                   aaaCAATGATGATCAGGAGAAAATTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAGCCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACAGTAACGAGACTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGACCAGAAAAACAAAAATGCACCTTGGTTTGTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGAc                                                                                                                                              <  2:99278/250‑1 (MQ=255)
                                                                                       aaTTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTTATCTTATTAAACCAGCAATCGACGCTCTCCGGAGGCAGATGACATTAACGAGACTGAGCAAAAAGCATATCATTGATGTTCCACT‑CAGAGAGTATGGCAGGACAGAAAAACAAAAATGCACCTTTGGTTTTCAACCTGAAATGTAAGCGAGAGTAAAAAATTATTGTGAACTTTTTACCGTTTACTCAAt                                                                                                                          >  1:277135/1‑251 (MQ=255)
                                                                                                                               cGGGAACCAAACGGGTCATTCATCTTATTAAGCCACCAATCGACGCTCTCCGGAGTCAGATTACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCGCCTTTGTTTTGCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTATACCTTTGACTCATTAATGCAGATGTGGGACTCAGCGATAAAACGTGCCAGACTc                                                                                  <  2:73203/251‑1 (MQ=255)
                                                                                                                                                        tattaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAg                                                             <  1:280178/247‑1 (MQ=255)
                                                                                                                                                         attaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCATAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGTACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCgag                                                        <  1:299892/251‑1 (MQ=255)
                                                                                                                                                         attaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACAGTAACGAGACTGAGCAAAGAGCATATCATTTATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGGTTGTCAACCGGATGTTTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGATTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCgag                                                        <  1:499330/251‑1 (MQ=255)
                                                                                                                                                            aaGCCAGCAATCGACGCTCTCCTTAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGAc                                                      <  1:46060/250‑1 (MQ=255)
                                                                                                                                                                 aGCAATGGTCGCTCTCCGGAGTCAGATGACATTACCGAGACTGAGCAAAGAGCATATCCTTGATGTTC‑ACCTAAGAGAGTATGGCAGAACAGAAAAACACAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGCCTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCAGGACTCCGCCATCGAAAATCATATAAGTCGAGACATACtt                                                >  2:186000/1‑251 (MQ=255)
                                                                                                                                                                          cGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATTGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGATTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCctgct                                       <  2:238876/251‑1 (MQ=255)
                                                                                                                                                                                                     gagaCTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCgg            >  1:280760/1‑251 (MQ=255)
                                                                                                                                                                                                      agaCTGAGCAAAGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGc           <  1:123751/251‑1 (MQ=255)
                                                                                                                                                                                                               aaaGAGCATATCATTGATGTTC‑ACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGCGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGc  <  2:106040/251‑1 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                  
GTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTC‑ACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGC  >  NC_000913/1652100‑1652557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑