Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,337,394	A→G	S522G (AGC→GGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,337,394	0	A	G	100.0%	161.6 / NA	56	S522G (AGC→GGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/29);  total (27/29)

CCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCA  >  NC_000913/1337150‑1337637                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    cccAAGTGTGCTGATGGCCGCAGTCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTTAAAGCGTCGCTTGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCTAAACTTACCTCGTATCTTGACGAACTGTT‑TTTGATCTGTGTGGGATACGGTTTTACCACCTGTATTGGTTAGTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGAtt                                                                                                                                                                                                                                               <  1:295882/251‑1 (MQ=255)  ccAAGTGTGCTGATGGCCGCAGGTGTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGAttt                                                                                                                                                                                                                                              <  1:454798/251‑1 (MQ=255)    aaGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAACGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTa                                                                                                                                                                                                                                             >  2:66941/1‑250 (MQ=255)       tgtgCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGTCCTCAAGCGGCAACCATGGGTCAAAGCGTAGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCg                                                                                                                                                                                                                                         >  1:497209/1‑251 (MQ=255)            tGATGGCCGCAGGCTTGCTGGCGCAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAACCGGCAATCAAAAAAGGCGATTTAACCGTcggt                                                                                                                                                                                                                                    >  2:59356/1‑251 (MQ=255)            tGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTcggt                                                                                                                                                                                                                                    >  1:111422/1‑251 (MQ=255)                   cGCATGCTTTCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGGTCGAAAGGCGTGTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGTGGTTTAACCT‑TGTGGGATACGGTTGTACCCCCTGTAGTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCggtgcggtgc                                                                                                                                                                                                                             <  1:45645/251‑1 (MQ=255)                    gCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCt                                                                                                                                                                                                                            <  1:45786/251‑1 (MQ=255)                         cTTGCTGGCGACAAAAGCAGTAACTCTGGGCCTCAAGCGGCAACCATGGGACAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCg                                                                                                                                                                                                                       >  2:462490/1‑251 (MQ=255)                                    aaaaaGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAAc                                                                                                                                                                                                            <  2:152816/251‑1 (MQ=255)                                                    gggCCTCAAGCGGCAACCATGGGTCAAAGCGTCTCTGGCACCGGTTTGGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGTGGTTTAACCT‑TGTGGTATACGGTTGTACCTCCTGTATGGGTAACTCTGGGCCGCTGCCCGTTCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTa                                                                                                                                                                                               <  2:479046/248‑1 (MQ=255)                                                            aGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGTGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTg                                                                                                                                                                                    <  1:347855/251‑1 (MQ=255)                                                                  aaCCATGGGTCAAATCGTCGCCGGCACCGGGTTCGAAAGTCGATTCTGATTATCTGGCAAACGCGAAACTGACACCGTATCTCGCCGAACTGGGGTTTAATCA‑TGTGGGATACGGTTGTACCACCTGTATTGGTGACTCTGGGCCTCTTCCCGATCCTATCGAAACGGCAAGCACAAAAGGCGAATTAACCGTCGGCGCGGTGCTGTCCGGCAACAGTAACTTGGAAGGCCGTATCCATCCTCTGGTTaac                                                                                                                                                                              >  2:268983/1‑250 (MQ=255)                                                                   aCCATGGGTAAAAGCGACGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGACACTGACACCGTATCTAGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTaaa                                                                                                                                                                              >  2:476526/1‑250 (MQ=255)                                                                            cAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActgg                                                                                                                                                                     <  2:88859/250‑1 (MQ=255)                                                                              aaGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActggctg                                                                                                                                                                  >  2:185385/1‑251 (MQ=255)                                                                               aGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActggctgg                                                                                                                                                                 <  1:20788/251‑1 (MQ=255)                                                                                   gcGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGGAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTc                                                                                                                                                             <  1:476526/250‑1 (MQ=255)                                                                                     gCTGGCACCGGGTTCGAAAGTAGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGGCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGACGTATCCCTCCGCCGGTTAAAACTAACTGGCTGGCCTcgc                                                                                                                                                           >  2:408861/1‑251 (MQ=255)                                                                                          cACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTcgccgccg                                                                                                                                                      <  2:256503/251‑1 (MQ=255)                                                                                               ggTTCGACAGTCGTTTATGATTATCTGGCAACAGAGAAACTGTAACCGTATCTCGACGAACTGGGGTTTACCCT‑TGTGGGATCCGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACAGTAACTTTGAAGGCCGTATCCCTCCGCTGGTTAAAAATAACTGGCTGGCCTCGCCGCAGCtggt                                                                                                                                                 >  2:437404/1‑251 (MQ=255)                                                                                               ggTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCACTCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtggg                                                                                                                                                 >  2:298408/1‑250 (MQ=255)                                                                                                gTTCGAAAGTCTTTTCTGATTATCTGGCAAAAGCGAACCTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGGACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGTTTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtggtg                                                                                                                                                <  2:150038/251‑1 (MQ=255)                                                                                                gTTCGAAAGTCGTTGCTGATTATCTGTCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtggtg                                                                                                                                                <  1:232025/251‑1 (MQ=255)                                                                                                                 gATTATCTGGCAAAAGCGAAACTGACATCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTgg                                                                                                                                <  2:125604/250‑1 (MQ=255)                                                                                                                   tcaTCTGGCCAAAGCGAAACTGACACCGTATCTCGACGAACTGGCGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGCTTTAACCGTCGGTGCGGTGCTTTCCGCCAACCATAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCCCGCCGCCGCTGGTGTTTGGCTATGCGCTTGCgg                                                                                                                             >  2:301326/3‑251 (MQ=255)                                                                                                                    tATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTCTGCGCTGGCggg                                                                                                                            >  1:8862/1‑251 (MQ=255)                                                                                                                                 cGAAACTGACACCGTATATCGCCGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATc                                                                                                               >  1:109597/1‑251 (MQ=255)                                                                                                                                  gAAACTGACACCGTATCTCGACGACCTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAAATGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGCAATATGAAAATc                                                                                                               >  2:99877/1‑250 (MQ=255)                                                                                                                                  gAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGACACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATc                                                                                                               >  1:338892/1‑250 (MQ=255)                                                                                                                                  gAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGCTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATc                                                                                                               >  2:259793/1‑250 (MQ=255)                                                                                                                                   aaaCTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCa                                                                                                              >  2:262833/1‑250 (MQ=255)                                                                                                                                     aCTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAAcc                                                                                                           <  1:59356/251‑1 (MQ=255)                                                                                                                                        gACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTgg                                                                                                        >  1:402639/1‑251 (MQ=255)                                                                                                                                           acCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCCACCTGGCtt                                                                                                     >  1:429474/1‑251 (MQ=255)                                                                                                                                                aTCTCGACGAGCTGGGGTTTAACCG‑TGTGGTATACAAATGTAACACCTGTATTGGTAACTCTGGCCCGCGGCCCGCTCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACAGTCGGTGCGGTGATGTCCGGCAACCGTAACTTTGAAGGCCGTATCAATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGATGCATATGCGCTGGCGGGACATATGACTATCCACCTGGCTGCTGa                                                                                                 >  2:414518/1‑250 (MQ=255)                                                                                                                                                 tctcGACGAACTGGGGTTTAACCT‑TGGGGGATATGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCGATCGAAAAGGCAAGTAAAAAAGGCGATTTAACCGTCGGTGCGGTGCGGTCCGGCAACAGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAAATGGCTGGCCTCGCTGCCGCTGGTGGTTGCCTATGCGCTGGCTGGAAATATGAATATCAACCTGGCTTCTGAg                                                                                                <  1:23194/250‑1 (MQ=255)                                                                                                                                                    cgacgaACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAg                                                                                                <  2:20120/247‑1 (MQ=255)                                                                                                                                                        gaACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCgg                                                                                        <  1:46188/251‑1 (MQ=255)                                                                                                                                                           cTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAACCGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGccc                                                                                     >  1:68685/1‑250 (MQ=255)                                                                                                                                                              gggTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCGGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGa                                                                                  <  1:185385/251‑1 (MQ=255)                                                                                                                                                                  ttAACCT‑TGTGGTATACGGGTTTACCACCTGTATTGGTAACTCTGGGCCCCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCTACCGTAACTTTGAAGGCCGTATCCATCCGCTGTTTAAAACTAACTGGCTGGCCTCTCCGCCGCGGGTGGTTGCCTATTCGCTGGCGGGAAAGATGAATATCAACCTGGCTTCTGATCCTATCGGCCATGATCg                                                                               <  1:30727/250‑1 (MQ=255)                                                                                                                                                                     aCCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGTTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCATCCGTAACTTTGAAGTCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGTTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCaaa                                                                           <  1:248910/251‑1 (MQ=255)                                                                                                                                                                       cT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCTCAAAgg                                                                         >  1:156940/1‑251 (MQ=255)                                                                                                                                                                          tgtgGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCACTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTTTCGGCCATGATCGCAAAgg                                                                         <  1:395702/249‑1 (MQ=255)                                                                                                                                                                             gggATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATc                                                                    <  1:491927/251‑1 (MQ=255)                                                                                                                                                                                       tGTACCACCTGTATTGGTAATTCTGGGCCGTTGCCCGATCTTATCGAAACGGCAATTAAAAAAGGCGATTTAGCCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTCTCGGCCATTATCGCAATGGCGATCTGTTTTATc                                                           <  2:355007/250‑1 (MQ=255)                                                                                                                                                                                                tGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGACACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGata                                                  >  2:96817/1‑250 (MQ=255)                                                                                                                                                                                                    ttGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTgg                                             >  1:225613/1‑251 (MQ=255)                                                                                                                                                                                                     tGGTAACTCTGGGCAGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTgg                                             <  1:46766/250‑1 (MQ=255)                                                                                                                                                                                                               gggCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTATCCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAGGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGcac                                   <  1:66941/250‑1 (MQ=255)                                                                                                                                                                                                               gggCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTCTCTGAAAGATATCTGGCCATCGGcac                                   >  1:198172/1‑250 (MQ=255)                                                                                                                                                                                                                       gCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTGTATCTGAAAGATATCTGGCCATCGGCACAAGAAAt                            <  1:178135/249‑1 (MQ=255)                                                                                                                                                                                                                                            aaTCAAAAAAGGCGCTTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGGAAGATATCTGGCCATCGGCACAAAAAATTGCCCGTGCGGTAGAACAAGtct     >  2:484573/1‑251 (MQ=255)                                                                                                                                                                                                                                               cAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCca  <  2:398955/251‑1 (MQ=255)                                                                                                                                                                                                                                               cAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCca  <  1:484573/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    CCCAAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCT‑TGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGTCTCCA  >  NC_000913/1337150‑1337637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑