Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,306,736	T→G	S325A (TCC→GCC)	oppF →	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,306,736	0	T	G	100.0%	56.1 / NA	21	S325A (TCC→GCC)	oppF	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/14);  total (7/14)

ATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTC  >  NC_000913/1306512‑1306976                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 atgaGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCg                                                                                                                                                                                                                        >  1:94323/1‑251 (MQ=255)                            cagacgtgtgctctccgatctCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGt                                                                                                                                                                                              <  1:297106/230‑1 (MQ=255)                                            gATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCAc                                                                                                                                                                             <  1:159814/251‑1 (MQ=255)                                              tttCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGTTCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGc                                                                                                                                                                           >  2:279747/3‑251 (MQ=255)                                                 cGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGCCAGt                                                                                                                                                                                              >  2:297106/1‑229 (MQ=255)                                                             agccTGATCCGGTTCTGGAGAATAACAAAACCACCCAGTATCTTGAAGTGGATTTCCCGTCGCCGATCTACCCGCCTTCCGGTTGTGTTTTCCTTTCCCGTTGCCCGATGGCCGGTCCAGAGTGCCCCAGATCACGTTCTGTTCGGGAGGGTTGTTACAGACACGCCTTTTCTTGGCTGAGGGTCTATCCGCTTTAAAAACAATAAGGGCTGACATTTGGCAGCTCTCTTTCATGCGAAATGCGAttattt                                                                                                                                                            <  2:219014/249‑1 (MQ=255)                                                              aCCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGTGAATTACCGTCGCCGATCAACCCGCCTTCCTGTTTTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGATTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGAttattt                                                                                                                                                            <  1:480435/250‑1 (MQ=255)                                                                   aTCCGGATCTGGAGAAGAACAAAACAATCCAGTTACTGGAAGGGGAATTACCGTCGCCGAACAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTccc                                                                                                                                                      >  2:490795/1‑251 (MQ=255)                                                                     ccGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCg                                                                                                                                                     <  1:489867/250‑1 (MQ=255)                                                                                    aaCAAACCCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCTTACCCGTTGCCCGATTGCCGGGCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTGAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCaa                                                                                                                                     <  1:311199/251‑1 (MQ=255)                                                                                    aaCAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCaa                                                                                                                                     <  1:277004/251‑1 (MQ=255)                                                                                      cAAAACCAGCCAGTTACTGGAAGGGGAATCACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTGCCGTCCCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCGTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAg                                                                                                                                   <  1:111323/251‑1 (MQ=255)                                                                                         aaCCACCCAGTTCCTGGAAGGGGAATTACCGGCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTGCCCGTTCCCGGATGGCGGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGAAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGCCAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGttt                                                                                                                                <  2:19462/251‑1 (MQ=255)                                                                                                                    cccGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATGGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGTCCTTTTTCACGCTAAAAGCGATTATTTATGCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGaa                                                                                                      <  1:34204/249‑1 (MQ=255)                                                                                                                    aCCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGaa                                                                                                      <  1:278767/250‑1 (MQ=255)                                                                                                                         cGCCGATCAACCCGCCGTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTTTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAATCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCggg                                                                                                <  2:336568/251‑1 (MQ=255)                                                                                                                              aTCAAACCACCTTCCGATTATGTTTACAGTACCCGCTGCCCAATTGCCGATCCTGAGTGCGCCACAACACGCCATGTTCTGGAGGGGAGATTCAGCCACGCCGTTTTTTGCCTGAACGTCGATCAGCTTTAAACACAATACGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTACCGCCAGATGATATGGAAACGTTCGTGATCTTTTTGGCGCCATAACAGAATGCAGGCaaa                                                                                            >  2:299480/1‑250 (MQ=255)                                                                                                                                cAACCCTCCTGCCGGTTGTTTTTTCCGTACCCTTTGCCCGATTGCCGGTCCAGAGTGCGTCAAAACACTTCCTGTTCTGGAGGGGATTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGTCTTACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAAc                                                                                         <  2:138188/251‑1 (MQ=255)                                                                                                                                     cGCCTACCGATTGTGCTTTCCGTCCCCGTTGCCAGCTTGCCCGCCCAGCGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCACACACGCCGTTTCTCGCCTGAACGTCGATCCGCTTTAAAAACAATACGGGCTGACAGTCGCCAGCCCTTTTTCACGCTCAAAGCGATTATTTATTCCCGCCACATGATATGGAAAAGATTGTGATCTTTTTCGCGGCATACCAGACTGCGGGCAACAACATCGt                                                                                    >  2:499556/1‑251 (MQ=255)                                                                                                                                                                       gATTGCCGGTCCAGAGTGCGCCAAACCACGTTCTGTGCTGGAGGGTATTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTGCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCAGCTTCACTGTCCGCCAGACCAAt                                                  <  1:279747/251‑1 (MQ=255)                                                                                                                                                                                                                       tttCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTc  >  1:321766/1‑251 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 ATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTC  >  NC_000913/1306512‑1306976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑