Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,992	A→T	N271Y (AAT→TAT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,992	0	A	T	100.0%	106.2 / NA	36	N271Y (AAT→TAT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (20/16);  total (20/16)

ACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGG  >  NC_000913/1301749‑1302230                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               actgcacctgggCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATGCCGTAAAAGAGTGGGTCGTAAACGAACTAAACGTTCTTGAACGCAGCCCGACCTAGTGGAACAGCGCGAAAACCTGTAGTAACCGGGTAACCTAGTTGCGGATTGCTTCTTAAGTGGCCGATGCCAACCGCTACCCGAGTTGTGAAACCGACATTACTTATAACAg                                                                                                                                                                                                                                         <  2:458662/240‑1 (MQ=255)   ttCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGc                                                                                                                                                                                                                                        >  1:280837/1‑250 (MQ=255)      aCCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACACAGCCTGGTAATATCGTCACCAACGGTGCCTCTACCTTAAACGCTTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGCCCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGc                                                                                                                                                                                                                                    >  1:206830/1‑251 (MQ=255)        cGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCCACGGTGCATATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCg                                                                                                                                                                                                                                  >  1:469528/1‑251 (MQ=255)          gTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACGGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGAt                                                                                                                                                                                                                                <  2:428560/251‑1 (MQ=255)                   gCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGtt                                                                                                                                                                                                                       <  1:335050/251‑1 (MQ=255)                         aTCGAGAAATTCGGCGAAAAATGGCCCCAACCTGGTAATATAGCCAGCACCGTTGCCTATAACTTCAAAGCTTGGATCGTAAACGAACGAATCGTTCTTGACCGCAGCCCGACCTACTGGCACAACGCGAAAACCGTTATTAACCAGGTAACATATTTGCATATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTCGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAgc                                                                                                                                                                                                                  >  2:35767/1‑249 (MQ=255)                             agaAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGACCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTg                                                                                                                                                                                                             >  1:330118/1‑251 (MQ=255)                                     ggctaaaaaTTGACCCAGCCTGGTAATAGCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGGCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGTAACAACGCGAAAACCGTTATTATCCATGTAACCTATTTGCCTATGGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAaga                                                                                                                                                                                                     <  2:386979/247‑1 (MQ=255)                                      gCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTAGTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAagag                                                                                                                                                                                                    <  1:48753/251‑1 (MQ=255)                                                         tGGTAATATCGTCACCAACGGTGCCTATACCTTAACAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAACTGCAAAAAGAGATCCCGGACGAAGATCACg                                                                                                                                                                                 >  2:384478/1‑251 (MQ=255)                                                             aaTATCGTCCCCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGCACGACTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTCACAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTAACGTTg                                                                                                                                                                              >  2:350171/1‑250 (MQ=255)                                                                          aCGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGGGGTGAAAGCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGGTGATCCATGCATGTGc                                                                                                                                                                <  2:507448/251‑1 (MQ=255)                                                                               tccTATACCTTAACAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAACGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACtt                                                                                                                                                            >  2:96521/2‑250 (MQ=255)                                                                                   ataCCGTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTAt                                                                                                                                                       <  2:399491/251‑1 (MQ=255)                                                                                               aTTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGATATCCCGGACGAAGTTCACGTTGATCCAGACCGGTGGACGCAGTATTGCGAAATTaac                                                                                                                                           <  2:206830/251‑1 (MQ=255)                                                                                                       gTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGa                                                                                                                                    >  1:319808/1‑250 (MQ=255)                                                                                                             gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGc                                                                                                                              >  2:142302/1‑250 (MQ=255)                                                                                                                 gaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTc                                                                                                                         >  1:277469/1‑251 (MQ=255)                                                                                                                      gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAACACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACg                                                                                                                     >  2:468936/1‑250 (MQ=255)                                                                                                                         cTTGAAAGCAGCACGAACTACTGGAACAACGCGAAAACCGTTATTAAACAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGAGGTGAAATCGACATGACGTATAACAGCATGCCGCTCGAATTGTTCCAGAAGCTGCAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGCtgt                                                                                                                 >  2:42747/1‑251 (MQ=255)                                                                                                                                 cAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgtgc                                                                                                         >  1:131425/1‑251 (MQ=255)                                                                                                                                         ccTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTAACCGATGACAACCGCTACCGGAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCa                                                                                                 <  2:15142/251‑1 (MQ=255)                                                                                                                                            aCTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTg                                                                                              >  2:112535/1‑251 (MQ=255)                                                                                                                                              gggAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACAGGACGTATAACAGCATGCCGATCGAATTTTGCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTGCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGaa                                                                                            <  1:338250/250‑1 (MQ=255)                                                                                                                                                           aaaCCGTTATTAACCAGGTAACTTATTTGCCTATTGCTTCGGAAGTTACCGATGTCAACCGCTACCTTAGTGGTGAAATCGACAGGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATGCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGAc                                                                               <  2:330118/251‑1 (MQ=255)                                                                                                                                                                  tattaACCAGGCAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGat                                                                         >  1:140172/1‑250 (MQ=255)                                                                                                                                                                          aGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTCCCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGtt                                                                <  1:413866/251‑1 (MQ=255)                                                                                                                                                                               cccTATTTGCTTTTTGCTTCTGAAGTTACCGATGTCACCCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTtaataa                                                           <  2:280837/250‑1 (MQ=255)                                                                                                                                                                                     ttGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTCCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGa                                                      <  1:75722/250‑1 (MQ=255)                                                                                                                                                                                              gCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATAGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAAGTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGCTAATAACGTGAAAGCGCAggg                                            >  1:497246/1‑251 (MQ=255)                                                                                                                                                                                                      aGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAAATGTTCCAGAAGCTGAAAAAAGAGATACCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATCTCATTGTTAATAAAGTGAAAGCGCCGGGCAACCTGc                                    >  1:203604/1‑251 (MQ=255)                                                                                                                                                                                                                  cacccgtacctgtggtggtaAAAGCGACATAGCGTATAACAGCATGTCGATCTAGTTGGTCAAGAACCTGAAAAAAGAGACCATGGACGAAGTGCTTGTTGATACGTACCTGTGCACTTCCTGTTAGGAATTTAACAACCAGATACCGCCATTCAACGATGTGTGGTTGCGTATCGCACTGATACTAGTTATGGACCGGTAGATCATTGTTAAGAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGtt                        <  1:262436/237‑1 (MQ=255)                                                                                                                                                                                                                               aGTGGTGAAATCGACAGGACGTAAAACAGCAGGCCAATCTAATTGTCCCAGAAGTTGAAAAAAGAGTTCCCGGACGAAGTTCACGTTGACCCATCCCTGTGCACTTTCTTTTCCAAAATTAACAACCAGAACCCGCCATTCAACGATGTGCGTGTGCGTACCACACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGt            <  1:317646/250‑1 (MQ=255)                                                                                                                                                                                                                                      aaaTCGACATGACGTATAACAGCATGCCGATCGCATTGTTCCAGAAGCTGAAAAAAGAGATCACGGACGTAGTTCACGTTGATCCATACCTGTACACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACAAGCCCGCCTATGGTTACCCTCCACCGTATACTGAt    >  2:605/1‑251 (MQ=255)                                                                                                                                                                                                                                        aTCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATgg  >  1:477117/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               ACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGG  >  NC_000913/1301749‑1302230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑