Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,098,620	T→A	V245V (GTT→GTA)	ghrA →	glyoxylate/hydroxypyruvate reductase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,098,620	0	T	A	100.0%	73.0 / NA	24	V245V (GTT→GTA)	ghrA	glyoxylate/hydroxypyruvate reductase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (17/7);  total (17/7)

GGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTTAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCTTGGCGGGAATTGAAT  >  NC_000913/1098379‑1098865                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      ggAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAATTTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAAGTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCACCCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTTGATAGCGGCAAAGTAAAAGgcg                                                                                                                                                                                                                                                <  1:470214/249‑1 (MQ=255)                                            cGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGCATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGGGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATCGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTc                                                                                                                                                                                                   >  2:35232/1‑251 (MQ=255)                                                                cATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCAcgc                                                                                                                                                                               >  1:478342/1‑251 (MQ=255)                                                                    tCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTg                                                                                                                                                                            <  1:505931/250‑1 (MQ=255)                                                                      tGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACg                                                                                                                                                                         >  2:382253/1‑251 (MQ=255)                                                                                          cttAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTcgccgc                                                                                                                                                     >  2:10286/2‑251 (MQ=255)                                                                                                     aCCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTc                                                                                                                                          >  1:365932/1‑251 (MQ=255)                                                                                                             ccccTGAAACCGTAGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCATTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGa                                                                                                                                   >  2:306489/1‑250 (MQ=255)                                                                                                                cTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCCCGCGGCGCTGGATAGCGGCAACGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCt                                                                                                                               >  1:498666/1‑251 (MQ=255)                                                                                                                  gAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCtg                                                                                                                              <  1:509102/250‑1 (MQ=255)                                                                                                                                           aaTTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGcc                                                                                                    >  1:5988/1‑251 (MQ=255)                                                                                                                                              tACTCGCACAATTACCGGATGGCGCGTATCTCCACAACCTGTCGCGTGGTCTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTCGATAGCGGCAAAGTAAAAGACGCAATGTTGGATGTTTTTAATCGTGAACCATTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGACCa                                                                                                  >  2:355504/1‑250 (MQ=255)                                                                                                                                              tACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGaa                                                                                                                                  >  1:361527/1‑218 (MQ=255)                                                                                                                                              tACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGGGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGAGGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGaa                                                                                                                                  <  2:361527/218‑1 (MQ=255)                                                                                                                                                  cGAAAAATTACCGGATGGCACGTATATCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGATGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACACTTACCGACTGAAAGTCCGCTCTGGCAACATCCACGAGTGACGATAACACCACATGTCGCCGCGATTACACGTCCCGCTGACGCTGTGGAGTACATTTCTCGCACCATTGCCAAGCTCg                                                                                             >  1:148614/1‑251 (MQ=255)                                                                                                                                                           acaggATGGCGCGTATCTCCTCAACCTGGCGCGTGGGGTTCATGCTGTGGAAGATGACCTACCCGCGGCGCTGGATAGCGGCAAAGTAAAATGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGACCGCTCTGGCAACATCCACGCGTGACGATAAAACCACATGTCGCCGCGATTACCCTTCCCGCTGAAGCTGCGGGGTACATTTCTCCCACCATTGACCATCTCGAAAAAgggg                                                                                    >  2:21335/4‑251 (MQ=255)                                                                                                                                                           aCCGGATGGCGCGGTTCTCCTCACCCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGTCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACTCGTGACGATAACACCACATGTCGCCGCTATTACCCGTCGCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAggg                                                                                     <  2:314300/250‑1 (MQ=255)                                                                                                                                                                 tGGCGCGTATCTCCTCAACCTGGGGCGTGGAGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAACGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGCTAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGATGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAggg                                                                              >  1:46949/1‑251 (MQ=255)                                                                                                                                                                                   ccTGGCGCGTGGGGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCACTGTTGGATGTTTTTAATCGTGAACCCTTACCGCATGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGCTTACCCGTCCCGCTGCAGCTGTGGAGTACCTTTCTCGCACCATTGCCAAGCTCGAAGAAGGGGGGAGGGTCTGCGGGCAAGTCGAcc                                                            >  1:203409/1‑251 (MQ=255)                                                                                                                                                                                               tgtTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGct                                                >  1:143928/1‑251 (MQ=255)                                                                                                                                                                                                                              gcgcTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCt                 <  1:510207/251‑1 (MQ=255)                                                                                                                                                                                                                                           ggaaaGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTTCCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTCCCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCGGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCTTGGCGGGAATTGa    <  2:278634/249‑1 (MQ=255)                                                                                                                                                                                                                                           gCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGAATAAGGATTCCTGCTATCCTTGGCGGGAAtgg     >  1:438521/1‑248 (MQ=255)                                                                                                                                                                                                                                             aaaGTAAAAGGCGAAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGCCGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCTTGGCGGGAATTGAAt  >  2:91814/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      GGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTTAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCTGCTATCCTTGGCGGGAATTGAAT  >  NC_000913/1098379‑1098865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑