Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 690,948 G→A V279V (GTC→GTT)  ybeX ← CorC‑HlyC family protein YbeX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913690,9480GA98.5% 222.9 / NA 68V279V (GTC→GTT) ybeXCorC‑HlyC family protein YbeX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (38/29);  minor base C (1/0);  total (39/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GCCAATAGCGGCAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTGACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTC  >  NC_000913/690702‑691193
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
gCCAATAGCGGCAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACAt                                                                                                                                                                                                                                                   >  1:384678/1‑251 (MQ=255)
       gCGGCAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGGCATCCGGGATTTTAACATGAACCTg                                                                                                                                                                                                                                            <  2:85017/251‑1 (MQ=255)
           cAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTCTCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGaat                                                                                                                                                                                                                                         >  2:367993/1‑250 (MQ=255)
           cAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCAGCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGGGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGaata                                                                                                                                                                                                                                        <  2:489053/251‑1 (MQ=255)
              aCTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCAACAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAataata                                                                                                                                                                                                                                     <  2:188926/251‑1 (MQ=255)
                  gAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGcc                                                                                                                                                                                                                                 <  2:236799/251‑1 (MQ=255)
                      ggACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGTCAAAATCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACt                                                                                                                                                                                                                             <  1:75596/251‑1 (MQ=255)
                      ggACGGCGGTTAAAGGGCAATGCCAGAAGCCCCAGCAGCGAAATAATCGCCGCAGGCCAGACGACGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCTAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTGATCTATCCAGTTTCGGTTTTATTCAGCCAGCTTCGGCTGGGGTGATTCATCCGGGATTTTAACATGAACCTGAATAATACGCCgtct                                                                                                                                                                                                                             <  2:351156/251‑4 (MQ=255)
                       gACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTg                                                                                                                                                                                                                            <  1:145370/251‑1 (MQ=255)
                            cggTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGTAGAGAAGGCCAGCGTTCCGCATGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTGTATTCATCCAGCTTCGGCTGGGGTGAGTCAGCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGc                                                                                                                                                                                                                       <  1:188152/251‑1 (MQ=255)
                             ggTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTACGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGCGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGcc                                                                                                                                                                                                                      >  2:432871/1‑251 (MQ=255)
                                     gTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGCGAGAAGGCCAGCGTTCCGCAGGCACAGCATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCAAGTTTCGGTTTTATTCATCCAGATTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGAATGTCGGCCATCGCCAc                                                                                                                                                                                                              >  2:118114/1‑251 (MQ=255)
                                     gTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATTCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCAc                                                                                                                                                                                                              <  1:327496/251‑1 (MQ=255)
                                     gTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACTTGAACCTGAATAATACGCCGACTGTCGGCCATCGCCAc                                                                                                                                                                                                              >  2:448642/1‑251 (MQ=255)
                                                           gCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCgtcgatg                                                                                                                                                                                        <  1:95829/251‑1 (MQ=255)
                                                            cGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCAGCAGGAACCGAATAATAACGCCAGAAACAGGAGAAAGCGCTGGCATTAAATTAATGTGGCCAAAGCCATGCAGTTACCTATCCAGTTTCGGTTTTATTCATCCAGCTTTGGCTGGGGTGAGTCATCAGGGATTTTAACATGAACCTGAATAATACGCCGCCTGTCGGACATCGCACCTTTGAACTGGTAACCgtcgatgt                                                                                                                                                                                       >  1:220525/1‑251 (MQ=255)
                                                                ataatCGCCGCAGTCCAGACTTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAAAAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCGTCGGCTGGGGTGAGTCAGCCGGGATTTTAACAGGAACCTGAATAATACGCCGACTTTCGTCCATCGCCACTTTGAACTGGTAACCgtcgatgtcgat                                                                                                                                                                                   <  2:337282/251‑1 (MQ=255)
                                                                  aatCGCCGCAGTCCAGTCGACGGAAGGAGAGAAGGGCAGCGATCAGCAGGCACCGAATAATAACGCCAGCAGCAGGGTAATTCGCTGGCGTTCAATTAATTAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGTCTGGGGTTAGTCAGCCGGGATTTTAACATGAACCTGAATAATACGCTGACTGTCGGCCATCTCCACTTGGAACTGGTACCCGTCGATGTCGATAg                                                                                                                                                                                 <  2:9903/251‑1 (MQ=255)
                                                                  aatCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAg                                                                                                                                                                                 <  2:311775/251‑1 (MQ=255)
                                                                                   cgtcgtAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATAGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCgg                                                                                                                                                                 >  1:507794/1‑250 (MQ=255)
                                                                                       gtAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGAt                                                                                                                                                            <  2:143255/251‑1 (MQ=255)
                                                                                       gtAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGAt                                                                                                                                                            <  1:446771/251‑1 (MQ=255)
                                                                                         aaGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGCGTCATCCGGGCTTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGc                                                                                                                                                          >  1:98700/1‑251 (MQ=255)
                                                                                           ggAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGccc                                                                                                                                                        <  1:219500/251‑1 (MQ=255)
                                                                                            gagagaAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGccc                                                                                                                                                        >  1:283269/1‑250 (MQ=255)
                                                                                                gaAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGCTAGTTTCGCCACGCGCCGGAAGATGCCCAAATg                                                                                                                                                   >  2:306315/1‑251 (MQ=255)
                                                                                                gaAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATg                                                                                                                                                   <  1:356735/251‑1 (MQ=255)
                                                                                                                  ggCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGaccac                                                                                                                                 >  1:435064/1‑251 (MQ=255)
                                                                                                                   gccccGAATAATAACGCCAGCAGCAGACGAATGCGCTGGCGTCCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGaccacc                                                                                                                                <  1:282502/248‑1 (MQ=255)
                                                                                                                    cACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCg                                                                                                                               >  1:10560/1‑251 (MQ=255)
                                                                                                                      ccGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGAGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGAt                                                                                                                             >  1:443912/1‑251 (MQ=255)
                                                                                                                        gAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGAt                                                                                                                             >  1:491112/1‑249 (MQ=255)
                                                                                                                         aataatAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGCCCACCGATAgt                                                                                                                          >  2:184548/1‑251 (MQ=255)
                                                                                                                          ataataACGCCAGCAGCAGGCGAATGCTCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAgtg                                                                                                                         <  1:432871/251‑1 (MQ=255)
                                                                                                                                  cccAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCTCCGATAGTGTCGActtc                                                                                                                 <  2:31224/250‑1 (MQ=255)
                                                                                                                                         gcagGCAACTGCGCAGACGTACAATTAATGAGGCCCAAGCCACGTAGTTATCTATCCCGTTTCGGTTTTATTAATCCAGCTTCGGCAGGGGGGAGACATCAGGGTTTTTAACATGAAACTGAATAATACGCCGACTGTCGGCAATCGCCACTTTGAACTGGTAACCGGCGATGTCGATAGTTTCGCCACACGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCCACTTCTTCaacg                                                                                                          >  2:360262/1‑248 (MQ=255)
                                                                                                                                           agGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCCCCAGACAACCGATAGTGTCGACTTCTTCATCGCt                                                                                                        >  2:294742/1‑251 (MQ=255)
                                                                                                                                               gcctGCGCTGACGTTCAATTAATGCGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATCAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGCTGCCCAACTGCCTGCATCACCAGACCACCGCTAGCTCCGACTTCTTCATCGCTac                                                                                                      >  2:417206/4‑248 (MQ=255)
                                                                                                                                               gAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGCTTTTAACATGAACCTGATTAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGCTAGTGTCGACTTCTTCATCGCTaa                                                                                                      >  2:10830/1‑249 (MQ=255)
                                                                                                                                               gAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTaa                                                                                                      >  1:127366/1‑249 (MQ=255)
                                                                                                                                                aaTGCGCTGGCGTTCAATTAATGAGGCAAAAGCCGTGTAGTTATCTATCCAGTTTCGGTTTTATTCATCAAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGCTAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAgg                                                                                                   >  2:209990/1‑250 (MQ=255)
                                                                                                                                                 aTGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGACGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTTCATCACCATACCACCGATAGTGTCGACTTCTGCATCGCTAAAggg                                                                                                  <  2:24653/251‑3 (MQ=255)
                                                                                                                                                      cTGGCGTTCACTTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGCGTCATCCGGGCTTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGACCTGGTAACCGTCTCTGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCCGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGTGTGc                                                                                             >  2:182446/1‑251 (MQ=255)
                                                                                                                                                          cGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGa                                                                                          >  1:317425/1‑250 (MQ=255)
                                                                                                                                                           tttCAATTAATGAGGCAACAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCAAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATTAACCTGCATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGCTAGTTTCGCCACGCCCCGTAATATGCCCAAATGCCTGCATCCCCAGAACACCGATCGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAAc                                                                                        >  2:316827/2‑251 (MQ=255)
                                                                                                                                                               aagtAATGAGGTAAAAGCCATGTAGTTATCTATCCAGTTTCGTTTTTATTCAGCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTTAAGAATTCGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGGCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCGGCATCGCCAGACCGCCGATAGTGTCGACTTCTTCATCGCTAAAGTTGGTGCCGAACGCtt                                                                                    <  2:346199/248‑1 (MQ=255)
                                                                                                                                                               aaTTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGCGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGCTAGTTTCGCCACGCGCCGGAAGATGCCCAAAAGCCTGCATCACCAGACCACCGAAAGTGTCGACTTCTTCATCGCAAAAGTGGGTGCCGACCGCtt                                                                                    >  1:230749/1‑251 (MQ=255)
                                                                                                                                                               aaTTAATGAGGCAAAAACCAAGTAGTTATCTATCCAGTGTAGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCCGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGAGTTCTTCATCGCTAAAGTGGGTGCCGAACGcgt                                                                                    <  2:359967/251‑3 (MQ=255)
                                                                                                                                                                     tGAGGCAAAACACATGCAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGGGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTg                                                                               >  2:356028/1‑250 (MQ=255)
                                                                                                                                                                      gAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGaa                                                                             <  2:273917/251‑1 (MQ=255)
                                                                                                                                                                              aGCCATGTAGTTCTCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGCATAATACGCCGCCTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCa                                                                      >  1:372464/1‑250 (MQ=255)
                                                                                                                                                                               gCCATGTATTTGTCTATCCAGTTGCGGTTTTATTCATCCAGCTTCGGCGGGGGTGAGTCAGCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGGAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCAGCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAAt                                                                    <  2:14800/251‑1 (MQ=255)
                                                                                                                                                                                    gTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGAATTCTTCATCGCTCAAGTGGGTGCCGAACGCTTCGTTGAAGTCttacatgga                                                                 >  2:424477/1‑242 (MQ=255)
                                                                                                                                                                                      aGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGcc                                                             >  1:130101/1‑251 (MQ=255)
                                                                                                                                                                                                ccAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGCTTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGCTAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAACGTGGGTGCCGAACGCTTCGTTGACGTCTTCAATGGACGCCAGTGCGCGt                                                    >  1:426316/1‑250 (MQ=255)
                                                                                                                                                                                                    tttCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGt                                               >  2:257750/1‑251 (MQ=255)
                                                                                                                                                                                                     ttCGGTTTTATTCATCCAGCTTCGGCTGGGGTGCGTCATCCGGGATTTTAACCTGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGTGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTTCGCGTACGGTc                                              >  2:155985/1‑251 (MQ=255)
                                                                                                                                                                                                       cGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTcc                                             >  1:128645/1‑250 (MQ=255)
                                                                                                                                                                                                       cGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTcc                                             <  1:397761/250‑1 (MQ=255)
                                                                                                                                                                                                          ttttATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGACGCCAGTGCGCGTACGGTCCAGGt                                         >  1:66402/1‑251 (MQ=255)
                                                                                                                                                                                                                 cATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATgacg                                   >  1:505862/1‑250 (MQ=255)
                                                                                                                                                                                                                  aTCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGACTAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATgacgac                                 >  2:39738/1‑251 (MQ=255)
                                                                                                                                                                                                                        tttCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGTATAATACGCCGACTGTCGGCCATCGCCACTTTGCACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGGGCCGAACGCTTCGTTGACGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCt                           >  2:8328/2‑251 (MQ=255)
                                                                                                                                                                                                                        cTTCGGCTGGGGTGAGTCATCCGGGATTTTCACATGAACCTGAATAATACGCCGGCTGGCGGCCATCGCCACTTTGAACTGGTAACCATCGATGTCGATAGCTTCGCCACGCGCCGGAAGATGCCCAAATCCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTATAGCGAGTGCCGGCCGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCATGCATGACGACTCAGCt                           >  2:453906/1‑251 (MQ=255)
                                                                                                                                                                                                                               gggggTGAGTCATCCGGGATTTTAACATGAACCTTAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCCCGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGGCGACTTCTTCATCGCTAAAGTGGGTTCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGGCCAGGTATGACGACTCAGCTTACGGaa                    <  2:311328/250‑1 (MQ=255)
                                                                                                                                                                                                                                       gTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCCTCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGatat            <  2:283269/251‑1 (MQ=255)
                                                                                                                                                                                                                                         cATCCGGTATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACCTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATatca          <  1:334589/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 aTTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATAATCttcttc  >  1:422696/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
GCCAATAGCGGCAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTGACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCTTCTTC  >  NC_000913/690702‑691193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑