Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 70,659 G→A W91* (TGG→TGA)  araC → DNA‑binding transcriptional dual regulator AraC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091370,6590GA96.2% 79.5 / NA 26W91* (TGG→TGA) araCDNA‑binding transcriptional dual regulator AraC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (16/9);  minor base C (1/0);  total (17/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAGCAATTGTTACTGCGGCGCATGGAAGCGATTAACGAGTCGC  >  NC_000913/70412‑70897
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              
tgctgcCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGatat                                                                                                                                                                                                                                             >  1:57162/1‑251 (MQ=255)
            aCTCGTTTAACGCTCATCGGGTGGCGGGTTTAACGCCGCTTGAGGCCAACGGTTATCTCGTTTTTTTTATCGTCCGCCCGCTGGGAAGGAAAGGTTATATTCTCAATCTACCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCGCGCGAATGATATCACCAGTGGGtt                                                                                                                                                                                                                                 <  2:182028/251‑1 (MQ=255)
               cGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGGCCGCTGGGAATGAAAGGTTATATTCTCAATCCCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGttt                                                                                                                                                                                                                                <  1:7115/249‑1 (MQ=255)
                    aaCGCCCATTGGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGTTATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTc                                                                                                                                                                                                                          <  2:191988/250‑1 (MQ=255)
                                                    tGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACt                                                                                                                                                                                         >  1:368428/1‑251 (MQ=255)
                                                          cAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGt                                                                                                                                                                                   >  1:380080/1‑251 (MQ=255)
                                                                   tctcGTTTTTTTTATCGACCGCCCGCGGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGTTGATATTTGGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATAttt                                                                                                                                                                           <  2:322787/250‑1 (MQ=255)
                                                                    ctcGCTTTTTTTATCGACCGCCCGCTGGGAATGCGAAGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGTATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCAACAGTGGGTTTACTTTCGGCCGCGCGCCTACTGGTATGTATGGCTTAAGTGGACGTCAATATTTGc                                                                                                                                                                         >  2:313492/1‑251 (MQ=255)
                                                                                    cccgaccgCTGGGAATGAAAGGTTATATTCTCAATCTAACCATTCGCGGTCAGTGGGTGTTGAAAAATCAGGTACGAGAATTTGTCTGCCGACCGGTTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTGCTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttcttt                                                                                                                                                         <  1:208087/250‑1 (MQ=255)
                                                                                             tGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGAGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGAt                                                                                                                                                >  1:426747/1‑251 (MQ=255)
                                                                                                                  tcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTc                                                                                                                           >  1:25891/1‑251 (MQ=255)
                                                                                                                   cAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCTGATGAAGCGCACCAGCCGCATTTc                                                                                                                           >  1:30802/1‑250 (MQ=255)
                                                                                                                       ctcACCATTCGCGGTCAGGGGGGGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGCCCGGGTGATATTTTGCTGTTCCCGACAGGAGAGATTCATCACTACAGTCGTCTTCCGGAGGCTCGCGAAAGATATCACCAGTGGGTTTACTTTCGACCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCTTCAATCTTTGCCAATACGGGTTTCTTTCGCCCGGACGAAGCGCACCAGCCGCATTACAGCgg                                                                                                                      >  2:416914/1‑250 (MQ=255)
                                                                                                                         ccccATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGc                                                                                                                        <  1:114485/245‑1 (MQ=255)
                                                                                                                                              ggtgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAACCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACg                                                                                               <  1:452067/251‑1 (MQ=255)
                                                                                                                                                 gaaaacccAGGGACGGGAAGTTGTCTGCCAACCGGGTTATATTTTGCTGTCCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGTAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGTAGCGCACCAGCCTCAGTTCAGCGACCTGTTTGGGCAAATCATTAACGcc                                                                                             <  2:355435/243‑1 (MQ=255)
                                                                                                                                                          gggacgggacattGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAAGAGTGCTTCATCACTACGGTCGCCATCCGGACACTCACGACTGATATCTCCAGTGGGTTTCCTTTCGTCCGCGCGCCTACTGGCATGAATGCCCTGACTAGCCGTCACTATTTGCCAATACGGGTTTCTTTCTCCCGGCTGAAGCCCCCCAGCCGCACTTCAGCGACCTGTTTGGGCAAATCATTAACACCGGGCACgggg                                                                                   >  2:245308/12‑251 (MQ=255)
                                                                                                                                                          gggACGAGAATTTGTCTGCCGACCGGGGGATATTTTGCTGTTCCCGCCGGGAGAGATTCACCACCACGGTCGTCATCCGGATGCTCGTGACTGATATCACCAGTGGGTTAACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCGGCATTTCAGCGGCCTGTTTGGGCAAATCAATAGCGCCGGGCACgggg                                                                                   >  2:34512/1‑251 (MQ=255)
                                                                                                                                                                 gaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAGGGGAAGGgcg                                                                            >  2:53854/1‑251 (MQ=255)
                                                                                                                                                                       gTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCCGGCGAGATTCATCACTACGGTCGTCATACGCCGGCTCGCGAATGCTATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTAACTATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGAACCAGCCGCACTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAGGGGAAGGGCGCTATTc                                                                      >  2:194929/1‑251 (MQ=255)
                                                                                                                                                                              gACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAGGGGAAGGTCGCTATTCGGAgctg                                                               <  1:429161/251‑1 (MQ=255)
                                                                                                                                                                               aCCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGCGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTACCTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGAACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAGGGGAAGGGCGCTATTCGGAgctgc                                                              >  1:71478/1‑251 (MQ=255)
                                                                                                                                                                                       atatTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGAt                                                       >  1:336492/1‑250 (MQ=255)
                                                                                                                                                                                                    cccGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAGc                                         >  1:125911/1‑251 (MQ=255)
                                                                                                                                                                                                            gagagaTTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAGCAATTGtt                                  >  2:291294/1‑250 (MQ=255)
                                                                                                                                                                                                             agagaTTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGCTAAATCTGCTTGAGCAATTGTTa                                 >  2:272944/1‑250 (MQ=255)
                                                                                                                                                                                                                                           ggCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAGCAATTGTTACTGCGGCGCATGGAAGCGATTAACGAGTCGc  >  1:141498/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              
TGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAGCAATTGTTACTGCGGCGCATGGAAGCGATTAACGAGTCGC  >  NC_000913/70412‑70897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑