Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,875,5320AT27.3% 49.3 / 11.2 33H32L (CAT→CTT) yidXputative lipoprotein YidX
Reads supporting (aligned to +/- strand):  ref base A (11/13);  new base T (9/0);  total (20/13)
Fisher's exact test for biased strand distribution p-value = 5.04e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCG  >  NC_000913/3875293‑3875777
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     
tactacAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCTTACCGCAAAgg                                                                                                                                                                                                                                            >  1:409761/1‑251 (MQ=255)
               cacaAAAAGAACAATTAATAACAAAAGGACATGCGTCGGGCAGAAAGCTGAAAAAGGATATAAGGCGATAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCGTAGCTACTCGTGTAAAGGATTAATCATGAAGTTTAATTGTAAGGTAGTTTTTAAGGCTTCTTGTTGATTCCCACTGGCGGTGATGCTTTCATGTTGTATCTCGTATGCTCTGGTTTCCCATACCGCATAGGGTAGTTCAGGAAAGt                                                                                                                                                                                                                             <  2:409761/251‑1 (MQ=255)
                                        aTGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTGTCTTTTGCCTTACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTAAGAAACCATCAc                                                                                                                                                                                                    >  1:86110/1‑251 (MQ=255)
                                            cATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCTCTAAACAGGATGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAATGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGAGGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATCCCGCAAAGTGTAGtgc                                                                                                                                                                                                                                     <  2:285116/214‑3 (MQ=255)
                                            cATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCTCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTc                                                                                                                                                                                                                                     >  1:285116/1‑214 (MQ=255)
                                                      gCAGAAATCTGAAAAGGGATATCAGGCGATAAACAGGAGGGAAAGAAGAGTATGCATTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAgg                                                                                                                                                                                      <  1:385799/251‑1 (MQ=255)
                                                               tGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAACAAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGAt                                                                                                                                                                              >  1:188062/1‑250 (MQ=255)
                                                                 aaaaGGGATATCAGGCGCTAAACAGGATGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAATTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATGCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGt                                                                                                                                                                           <  1:306148/251‑1 (MQ=255)
                                                                                                     gaggagggtatCAACGGCTTAGCTTCTCGTTTAAAGGATTAATCATGAAGATAAAGTTTAAGGGATTTTTTAAGGCTGCCGGTTTATGCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCg                                                                                                                                       <  1:61752/241‑1 (MQ=255)
                                                                                                           gCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTCTCCCTTACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGCCACCATCACTGGGCTATCGCAGGCAAACGCTAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGAt                                                                                                                                 >  1:94344/1‑251 (MQ=255)
                                                                                                              ttCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTCTGTTTTTGTAGGGGATTCGCTGGTTTAc                                                                                                                              >  2:284453/1‑251 (MQ=255)
                                                                                                                          gctcctcatttAAAGGATTAATCATGAAGTTGAATTTTAAAGTATTTTTTAAGGAGGCCGGTTTATTTCCACAGGCGCTGATGCTGTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGTTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGGAGGGGAATCGCTGGATTTCCTTATCTCTGAt                                                                                                                  <  2:101471/243‑1 (MQ=255)
                                                                                                                           cTACTCGTTTAAAGGATTACTCCTGAAGCTGAATTTTAAGGGATTTTTTAAGACTGCCGGTTTATTACCAATGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATg                                                                                                                 >  2:61770/1‑251 (MQ=255)
                                                                                                                                       aGGATTAATCATGACGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGAATCCCGTATGCTCTGGTTTCCTATACCCCCAAAGGCCGTACAGGAAAGTATCAATCCCAGCCAGACACCATCACTGAGCTATCGCAGGCAAACGATAGTACTGGACCCAAAGGCTATGTTTTTGTAGGGGACTCGCTGGATTACCTTATAACTGATCGTGCCGATGAc                                                                                                      >  1:334720/1‑250 (MQ=255)
                                                                                                                                           ttAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTGTGTTTTGCCTTACCGAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATAGCAGGAAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGt                                                                                                 >  1:361913/1‑251 (MQ=255)
                                                                                                                                                 aTGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTCTTTTCCCAGAAAGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTa                                                                                               >  1:168006/1‑247 (MQ=255)
                                                                                                                                                   gAAGTTGAATTTTAAGGGATTTTTTAAGGATGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACACAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGc                                                                                         >  1:146042/1‑251 (MQ=255)
                                                                                                                                                       ttGAATTTTAAGGGATTTTTTACGGCTGACGGTTTATTCCCACTGGCGCTGATGCTTTCCGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCa                                                                                      >  2:56728/1‑250 (MQ=255)
                                                                                                                                                                aaGGGATTTTTTAAGGCTGCCGGTTTATTGCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAg                                                                             <  1:281580/250‑1 (MQ=255)
                                                                                                                                                                aaGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATCGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCAGATTACATCGTTAAGATGCTCAATGATCCAGc                                                                            >  2:14777/1‑251 (MQ=255)
                                                                                                                                                                           tAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTTTGCCTTAACGCAAAGGGAAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAACAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTTCAGATGACATAGTTAAGATGCTCAATGATACAGAACTTAAcctgc                                                                 >  1:348062/1‑248 (MQ=255)
                                                                                                                                                                               gatGCCGGTGTATTTCCACGTGCGCTGATTATTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCACAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGTAACAAAAGGCTATGTTTTTTTAGGGGAATCGCTTGAGGTCCTTATCACTGATGGTGCCGATGACATCGTTAATATTCTCAATGAGCCATCACTTAACCGGCACaa                                                             <  2:362812/249‑1 (MQ=255)
                                                                                                                                                                                      gtgtATTCCCACTGTAGCTGAGGCTTTCATGCTGTATCTCGAATGCTCTGGTATCCAATACCGCAAAGGATAGTGTAGGAAAGTATCAATCGAAGTCATACACCATCACTGGGCTAGCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAAGCGCTGGATTTCCTTATCACGGATGGTGCCGATGACTTCGTTAAGATGCGCAATGAGCCAGCACTTAACCGGCACAATATTCAg                                                      <  2:447149/248‑1 (MQ=255)
                                                                                                                                                                                       tttATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGTTTTCCCTTACCACAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCCGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGCATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAgg                                                     >  1:197162/1‑251 (MQ=255)
                                                                                                                                                                                          aTTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTTTGCTTTAACGAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTg                                                  >  1:109610/1‑251 (MQ=255)
                                                                                                                                                                                             cctccTGGCGCTGATGCTTTCAGGCTGTATCTAGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCg                                               <  2:145205/247‑1 (MQ=255)
                                                                                                                                                                                               cACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTCTTTTCCCTGACCGCAAAGGGTAGTTCAGGAAAATATCAATCGCAGACAGACACCATCGCTGGGCTATCGCAGGCAAAAGGTAGTAATGTACCAAAAGGCTATTTTTTTGTAGTGGAATCGCTGGATTACCTTATCACTGATGATGCTGATGACATCGTTAAGTTGCTGAATGTTATAGCACTGAACAGACACAGTATTCAGGTTGcgga                                              >  1:384971/1‑247 (MQ=255)
                                                                                                                                                                                                            gCTTTCAGGCTGTATCTCGTATGCTCTTGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATCGTAATGGAACAAAAGGCTATGTTGTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCTTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATAGTCAGGTTGCCGATGACGCAAGAttt                                 <  2:103137/250‑1 (MQ=255)
                                                                                                                                                                                                                 cAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGGATCAAGCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGGTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGAGCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGtttt                            <  1:39471/250‑1 (MQ=255)
                                                                                                                                                                                                                               tattatctGGTTTCCCATACCGCAAATGTTAGTTTAGGAAAATATCAATCGCAGTCAGACAACATAACTGGGCTATCGCAGGCAAGAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAGTCGTTGGATTACCTTATCACGGAGGTTGCCTATGACATCGTTAAGATGCACAATGATCTAGCATTTAACCGGCACATTATTCTGGTTGTCGATGACGAAAGATTTGTTGGTAATGCGGGGaaaa              <  2:222670/245‑1 (MQ=255)
                                                                                                                                                                                                                                   ctctGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGaa          >  1:300753/1‑250 (MQ=255)
                                                                                                                                                                                                                                         tttCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTAcc   >  2:132564/1‑251 (MQ=255)
                                                                                                                                                                                                                                          ttCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCg  >  1:141194/1‑251 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     
TACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCG  >  NC_000913/3875293‑3875777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑