Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,908,726	IS2 (–) +5 bp	coding (37‑41/144 nt)	mgrB ←	PhoQ kinase inhibitor

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1651548	NA (NA)	33 (0.680)	30/494	0.6	100%	noncoding (706/706 nt)	IS2	repeat region
	?	NC_000913	= 1908730	0 (0.000)					coding (37/144 nt)	mgrB	PhoQ kinase inhibitor
*	?	NC_000913	1908726 =	0 (0.000)	40 (0.820)	35/494	0.4	100%	coding (41/144 nt)	mgrB	PhoQ kinase inhibitor
	?	NC_000913	= 2070271	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region

GCTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGACTCCCTATATCACTTAAATTGATACAACTTTTTA  >  NC_000913/2070027‑2070516                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      gCTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAACCTAGTATTAAAACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGGGGCg                                                                                                                                                                                                                                                 >  1:205085/1‑251 (MQ=11)  cTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCg                                                                                                                                                                                                                                                 >  1:301409/1‑250 (MQ=31)            agaAATAACTGACCGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCAtcctcc                                                                                                                                                                                                                                      >  1:355981/1‑251 (MQ=25)                    ctggctggCTGCTACACCCTGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGtttt                                                                                                                                                                                                                              >  1:97147/1‑251 (MQ=255)                              gctACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCAt                                                                                                                                                                                                                     <  2:111369/250‑1 (MQ=255)                               ctACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTACGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTg                                                                                                                                                                                                                   >  1:100833/1‑251 (MQ=255)                                                             ccGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAATCTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCg                                                                                                                                                                                     <  1:296570/251‑1 (MQ=255)                                                              cGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATAATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCgg                                                                                                                                                                                    >  1:351086/1‑251 (MQ=255)                                                              cGTCATCCCCGGTCCAAAGCTCTGCCGAACAATTGCGCTCTTTTCCTGTCTGGTACGCCGTCTGCGTCTCTCCGGCCATCAGACATCACTCATCTGTTCTCCACTGACTCGTCTACAAACTAGTATTAAGACTATCACTTATTTCCGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCgg                                                                                                                                                                                    >  2:262066/1‑251 (MQ=18)                                                                                                               gtgGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCg                                                                                                                                    <  1:92122/250‑1 (MQ=255)                                                                                                                             gCGTTTCACCGGCCCTAATACATCAATCATCGGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTAGCACTTATGTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCTAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAAGTAACTTATCAGCGCTCCGGAGATAATTATGGTGTGGGACTTTCGATAGATGAGTCTGCGCTGTAGCGTGTTGAGGTAGt                                                                                                                        <  2:473895/248‑1 (MQ=25)                                                                                                                                     ccGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTcc                                                                                                               <  1:253388/249‑1 (MQ=255)                                                                                                                                         cccTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGATGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTc                                                                                                         >  1:101819/1‑251 (MQ=255)                                                                                                                                              aGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTAc                                                                                                    <  1:321905/251‑1 (MQ=255)                                                                                                                                                    caatcaTCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACg                                                                                              <  2:301113/251‑1 (MQ=255)                                                                                                                                                          tcTGTTCTCAAGTGACTAGTCTAAAAACTAGAATTCAGAATATCACTTATTTACGTGCTATTGATTGTCTGGAGATTCAGGGACCCAGTATATTGGCGAATAATCCTCCCGTGTTTTACTTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGTTAATTATGGTGTGGGACTGTTGATACATGAGTCTGCGCTGGAGCGTGTTGAGGCAGCGAAAGGTCCATATTCCGTACTGTAAGGTTccc                                                                                        >  2:333699/1‑249 (MQ=255)                                                                                                                                                             gTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGTAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTcaca                                                                                       <  1:262066/249‑1 (MQ=255)                                                                                                                                                               tctcCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGc                                                                                    <  1:231976/250‑1 (MQ=255)                                                                                                                                                                 tcCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAAt                                                                                 >  1:344335/1‑251 (MQ=255)                                                                                                                                                                                     cTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTACCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAAATTATCAGCGCGCAGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTTTTAACTtc                                                              >  1:515369/1‑250 (MQ=255)                                                                                                                                                                                         tATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCAc                                                         >  1:107780/1‑251 (MQ=255)                                                                                                                                                                                                              aaGTGATATTGGTGTTCTGGAGATTTGGGGGGCCAGTCTAGTGGCGAATCATCTTCCCGTGTTTTAATTC‑CAATGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGa                                    <  1:496789/250‑1 (MQ=255)                                                                                                                                                                                                               aGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGAc                                   >  1:1591/1‑251 (MQ=255)                                                                                                                                                                                                                   atatTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCACAAAGGGTGAATCTCCGGACTccc                               >  1:501023/1‑251 (MQ=255)                                                                                                                                                                                                                       tGGTTGTCTGGAGATTCAGGTGGCCAGTCTAGTGGCTAAGCATCCTCCCGTTTTTTAATTCTCATTGATGGGCAGGAGGTAACTTATCAGCGCGCCGGAGATAAGTAGGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGGGCGGTTTGGGGTAGTGAAAGGTCCATATGCCGTACTGTACGGTTCACAGGCTGTTGGCGGTATTGGTAACTTCATCACCAAAAAGTGTGAATCTCCGGATTCCCtat                            <  2:331067/250‑1 (MQ=255)                                                                                                                                                                                                                          gtGTCTGGAGATTCAGGGGGCCAGGCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATGATGGTGTGGGACTGGTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGACTCCCTATATCa                        <  1:117979/250‑1 (MQ=255)                                                                                                                                                                                                                            gTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGACTCCCTATATCACt                      >  1:169121/1‑251 (MQ=255)                                                                                                                                                                                                                                     ttCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGACTCCCTATATCACTTAAATTGAt             >  1:303582/1‑251 (MQ=255)                                                                                                                                                                                                                                       cAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAAGTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGACTCCCTATATCACTTAAATTGATAc           <  1:104938/251‑1 (MQ=255)                                                                                                                                                                                                                                        aGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAACGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGGGAATCTCCGGGCTCCCTATATCACTTAATTTGATAc           >  1:493862/1‑250 (MQ=255)                                                                                                                                                                                                                                             gCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCTATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCACAAAGGGTGAATCTCCGGACTCCCTATATCACTTAAATTGATACAACttt     >  2:233146/1‑251 (MQ=255)                                                                                                                                                                                                                                                aGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGACTCCCTATATCACTTAAATTGATACAACTTTTTa  <  1:314114/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      GCTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGACTCCCTATATCACTTAAATTGATACAACTTTTTA  >  NC_000913/2070027‑2070516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.