Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,468,298	IS3 (+) +3 bp	coding (85‑87/921 nt)	yfdH →	bactoprenol glucosyl transferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2170171 =	NA (NA)	31 (0.640)	29/494	0.6	100%	noncoding (1/1258 nt)	IS3	repeat region
	?	NC_000913	= 2468300	0 (0.000)					coding (87/921 nt)	yfdH	bactoprenol glucosyl transferase
*	?	NC_000913	= 2171428	NA (NA)	41 (0.840)	39/492	0.3	100%	noncoding (1258/1258 nt)	IS3	repeat region
	?	NC_000913	2468298 =	0 (0.000)					coding (85/921 nt)	yfdH	bactoprenol glucosyl transferase

GTATGAGCGCAAAAGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGT‑CTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTC  >  NC_000913/2171181‑2171675                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                         gTATGAGCGCAAAAGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAg                                                                                                                                                                                                                                                         >  1:314794/1‑249 (MQ=11)      aGCGCAAAAGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATccc                                                                                                                                                                                                                                                  >  1:208450/1‑251 (MQ=33)              aGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTggg                                                                                                                                                                                                                                          >  1:386075/1‑250 (MQ=25)                      gctACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGACCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGGAGGATCAGTCATCCCTTCGTGGATCATTTTg                                                                                                                                                                                                                                  >  1:390646/1‑251 (MQ=255)                         aCGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACt                                                                                                                                                                                                                               >  1:291901/1‑251 (MQ=255)                         aCGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAAGGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGGTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACt                                                                                                                                                                                                                               <  1:199415/251‑1 (MQ=255)                            aTAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGCGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCCGTCCGGGACAATTTGCAAACAAGAACCTCGCTTAGGCCTGTGTCCTTATTACGTGGGTAGGATCAGTCATCCCTTCGCTGATCATTTTGACTgcc                                                                                                                                                                                                                            >  2:153525/1‑251 (MQ=14)                                         ggAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTAtt                                                                                                                                                                                                                <  1:227299/250‑1 (MQ=255)                                         ggAAAGCTTCTTTAATTCGCTGAAAGTGGAAAGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCCGTCCCGCACAATTTGAAAACAAACACCTCGCTTAGGCCTGTGTCCATATTACGTGGGGAGGGACAGTCATCCCTTCGCGGATCATTTTGACTGCCGCCTGCGCTATTc                                                                                                                                                                                                               >  2:335279/1‑251 (MQ=14)                                           aaaGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCCGTCCTCCCTTCGTGGATCCTTTTGACTTCCGCCTGCGCTATTCTg                                                                                                                                                                                                             >  1:421729/1‑251 (MQ=255)                                                 tcttTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGttttt                                                                                                                                                                                                        <  2:208450/250‑1 (MQ=255)                                                  cttTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGGCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATTTTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGTCTGCCGCCCGCGCTATTCTGttttttt                                                                                                                                                                                                      >  2:364282/1‑250 (MQ=255)                                                     tCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGgc                                                                                                                                                                                                    <  1:402424/250‑1 (MQ=255)                                                            cTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGc                                                                                                                                                                                            >  1:399013/1‑251 (MQ=255)                                                              gaaatttgAATGTATCCATGGAGAACACTGTATCAGCCGGGAAATAATGCGGGCAACGGTGGTTAATTATATCGAATGTGATTACAAGCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAATAACCTCGCGTAGTCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATGCTGTTTTTTGgcgctttggcg                                                                                                                                                                                           <  2:409739/243‑8 (MQ=17)                                                              gAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGc                                                                                                                                                                                            <  1:294675/249‑1 (MQ=255)                                                                  gTGGAATGTATCCATGGAAAACTCTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTACATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTg                                                                                                                                                                                      <  2:139652/251‑1 (MQ=255)                                                                   tGGAATGTAGCCATGAAGAACACTTTATCAGCCGGGAACTAATACGGGCATCGGTGTTTAATTATATCGAATGTGATTACAATCGTTGGCGGCGGCACAGTTGGTGTGGCGGGCTCAGTCCGGAACAATTTGAAAACAAGAAACTCCCTTAGGCCTGTGTGCCTCTTACGAGGCGCGGATGAGTCCTCCATTCGAGGGTCATTTTGTCTGCCGCCCGCGCTCTTCTGTTTTTTGTGGCGCTgtcaacgtg                                                                                                                                                                                      >  2:145427/1‑242 (MQ=2)                                                                       aTGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATc                                                                                                                                                                                 <  2:264502/251‑1 (MQ=255)                                                                               aTGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTa                                                                                                                                                                         <  1:371595/251‑1 (MQ=255)                                                                               aTGGAGAAAACTTTCTCAGCCGCGAAATAATGCGGGCAACGGAGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAAAAAGAACCTCGCTTATGCCTGTGTCCCTATTACTTGGGTAGGATCAGTCATCCCCTCGTGGATCATTTTTACTACCGCCTGCGCTATTCTGTTTTTTGGCGCGCTgacggtctacaatcgcttttt                                                                                                                                                                          >  2:357221/1‑230 (MQ=17)                                                                                     aaCACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCg                                                                                                                                                                   <  1:105522/251‑1 (MQ=255)                                                                                                 gCCGGGAAATACTGCGGGCAACTGTTTTTAATTATCTCGAATGTGATTACAAGCGGTGGCGGCGGACCTGTTGGTGTGGCTGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTTCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCCCTTTAAACCCGACTTCCTGGGt‑c                                                                                                                                                      <  2:4515/251‑1 (MQ=21)                                                                                                    gggAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATTCCTTCGTGGATCATTTTTACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGT‑TTCg                                                                                                                                                   >  2:94056/1‑251 (MQ=255)                                                                                                    gggAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGT‑CTCg                                                                                                                                                   <  1:186803/251‑1 (MQ=255)                                                                                                     ggAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTgc                                                                                                                                                                                                                      >  1:235282/1‑184 (MQ=255)                                                                                                     ggAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTgc                                                                                                                                                                                                                      <  2:235282/184‑1 (MQ=255)                                                                                                       aaaTAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTATGCCTGTGTCCATATTACGTGGGGAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAAACCGACTTCCTGGGG‑CTCGGtt                                                                                                                                                >  1:417193/1‑251 (MQ=255)                                                                                                                       ggTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTACGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGGCCCTATTAAGTGGGTAGGATCAGACATCCCTTCGGGGATCATTTTGACTTCCGCCTGCGCTATTCTGTTTTTTGGCTCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGCCTTTCTGGGT‑CTCGGttttcctatttcacgac                                                                                                                                 >  2:26687/1‑236 (MQ=21)                                                                                                                                 tataTCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGT‑CTCGGTTTCACTATTACCAGACATTATTTTcg                                                                                                                       <  1:349457/250‑1 (MQ=255)                                                                                                                                   tatCGAATGTGATTACAATCGGGGGCGGAGGCACAGTTGGTGTGGCGGCCTCAAATCGGAACAATTTGAGAACGAGAACCACGCTTAGGCCTGTGTCCGTATTTCGTGGGTAGGATCAGGCCTCCCTTCGTGGATCATTTTGACTGCCGCCGGCGCTCTTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGT‑CTCGGGTTCCCTCTTACCGGACATTATTTTcgccc                                                                                                                    >  2:375908/1‑250 (MQ=255)                                                                                                                                    atCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGT‑CTCGGTTTCACTATTACCAGACCTTATTTTcgccgc                                                                                                                   >  1:417201/1‑251 (MQ=255)                                                                                                                                         aTGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGACTCAGTCCGGAAAAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCATCCCTTCGTGGATCATTTTGACTGCCGCTTTCGCTATTCTGTTTTTTGGCGCGCTGGCGGGCTGAAATCGCTCTTTAAACCCGACTTCATTGGT‑CTCGGTTTCACTATTACCTGACATTTTTTTCGCCGCACCGc                                                                                                              >  2:104902/1‑251 (MQ=25)                                                                                                                                                 aCAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGT‑CTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGaa                                                                                                        >  1:70015/1‑249 (MQ=255)                                                                                                                                                     tCGGTAGCGGCGGCACAGTTGGTGTGGCGACCTCAGTCCGGCACAATTTGCAAACAAGCACCTCGCTTACGCCTGTTTCCATATTAAGCGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTTCCTACCGACTGAGCTATTCTGTTTTTTGGCGCGCTGGCGCGCTGAAATCCCTCTTTAAACCCTACTTCCTTGGT‑CTCGGCTTCACTATTACCCGACATTTTTTTCGCCGCACCGCCATGACAGcgcg                                                                                                  >  2:420004/1‑251 (MQ=255)                                                                                                                                                        gTGGCGGCTGCACATTTGGTGGTGCGCCCTCAGACCGGAACAATTTTAAAAAAAGACCCTCGCATAGGTCTGTGACAATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCTTTTTGACTTCCGCTTGCGTTATTCTTTTTTTTGGCTCGTTGGCTATCTGAATTCGCTCTTTAAACCCGCCTTC‑TGGGTTCTGGGTTTCACTAATCCCAGATATTTTTTCCCCCGCTCCTCCATGCAAGCGCGTaa                                                                                               <  2:424412/251‑1 (MQ=255)                                                                                                                                                            cgggggcccatttGGTGTGGTGGCCACAGTCCGAAACAATTTGAAAACAAGAACCTCGCTTAGGCCTTTGTCCTTATTCCGTGGGGAGGATCGGTCATCCCTTCGTGGATCATTTTGTCTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGGGAGCTGAAATCGCCCTTTAAACCCGACTTCCTGGGT‑CTCGGTTGCACTATTACCAATCATCATTTTCGCCGCACCGCCATGAAAGCGCGTAACCaa                                                                                           <  2:107513/240‑2 (MQ=255)                                                                                                                                                               cggcACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGGGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGT‑CTCGGTTTCACTATTACCAGACATTATTTTCGCCGCCCCGCCATGAAAGCGCGTAACCACGcc                                                                                        >  1:170849/1‑251 (MQ=255)                                                                                                                                                                    cggTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGT‑CTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCttttt                                                                                   <  1:157000/249‑1 (MQ=255)                                                                                                                                                                              ggcggcCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGT‑CTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGACAGCGCGTAACCACGCCTTTTTGTTCGAGaa                                                                          >  2:288910/1‑250 (MQ=255)                                                                                                                                                                                  gcCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGT‑CTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCTCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAgcgc                                                                     >  2:287891/1‑251 (MQ=255)                                                                                                                                                                                          ccggaaaaagtggAAAACAAGAACCTCCCTTTGGCCTGTGTCCATATTACGGGGGTAGGTTCAGTCATCCCTTCGTGGACCATTTTGACTGCCGCCTGCTCTATTCTTTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGTT‑CTCGTTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGc                                                             <  2:417531/239‑1 (MQ=255)                                                                                                                                                                                                   tttGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGT‑CTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGt                                                    >  2:134900/1‑251 (MQ=255)                                                                                                                                                                                                                       ttAGGCGTGTGTCATTATTCCGGGGGTAGGACCAGTCTGCCCTTCTTGGATCATTTTGACTGCCGCCTGCGCTTTTCTTTTTTTTGGCGCGCTGGCGAGCTAAAATCGCTTTTTAAACCCGTCGCCCTGGGT‑CGCGGTTTCACTATTCCCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAAt                                 <  2:386075/250‑1 (MQ=255)                                                                                                                                                                                                                              tgtgTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGT‑CTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGc                         >  1:7791/1‑251 (MQ=255)                                                                                                                                                                                                                               gtgtCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGT‑CTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGcc                        >  2:459563/1‑251 (MQ=255)                                                                                                                                                                                                                                       atTACGGGGGTAGGACCAGTCATCCCTTCGTGGATCATTTGGACTGCCCCCTGCGCTTTTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGCCTTCCTGGGT‑CTCGTTTTCACTATTACCAGACATTATTTCCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTg                <  1:134900/251‑1 (MQ=255)                                                                                                                                                                                                                                        ttACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGT‑CTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTg                >  2:116763/1‑250 (MQ=255)                                                                                                                                                                                                                                             ggggTAGGATCAGTCACCCCTTCGGGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCGGGGT‑CTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAg          <  2:70015/250‑1 (MQ=255)                                                                                                                                                                                                                                               ggTAGGATCAGTCTTCCTTTCGTGGATCATTTTGCCTGCCGCCTGCGCTATTCTGTTTTTGGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGT‑CTCGGTTTCACTATTCCCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCa        <  1:385464/251‑1 (MQ=255)                                                                                                                                                                                                                                                   ggATCAGTCACCCCTTCGTGGATCTTTTTGCCTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGTGGGCGAGCTGAAATCGCTCTTAAAACCCGACTCCCTGGGT‑CTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGTCAGCAAATACGCCCGCCAGATCCTGAACAAGCACGGt    <  1:401698/251‑1 (MQ=255)                                                                                                                                                                                                                                                     aTCATTCATCCCTTCGTGGTTCTTTTTGACGCCCCCCTGGGTTTTTCTGTTTTTGGGCGCGTTGGCGAGCTGAAATCGCTTTTCAACCCCGACTTCCTGGGC‑TTCGTTTTCATAATTCCCAGACAGTATTTTCGCCCCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGGTCGAGAAAGCGCAAATCGGTACGGATTGTCGCTTCCGAGGCAGCAAATTCTCCCGCCAGATCCTGAACAAGCACGGTTc  <  2:220350/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                         GTATGAGCGCAAAAGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAGTCATCCCTTCGTGGATCATTTTGACTGCCGCCTGCGCTATTCTGTTTTTTGGCGCGCTGGCGAGCTGAAATCGCTCTTTAAACCCGACTTCCTGGGT‑CTCGGTTTCACTATTACCAGACATTATTTTCGCCGCACCGCCATGAAAGCGCGTAACCACGCCTTTTTGTTCGAGAAAGCGCAAATCGGCACGGATTGTCGCTTCCGAGGCAGCAAATACTCCCGCCAGATCCTGAACAAGCACGGTTC  >  NC_000913/2171181‑2171675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.