Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,212,973	Δ1 bp	intergenic (+27/‑67)	rrlE → / → rrfE	23S ribosomal RNA/5S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,212,973	0	T	.	100.0%	15.9 / NA	6	intergenic (+27/‑67)	rrlE/rrfE	23S ribosomal RNA/5S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (3/3);  total (3/3)

GTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCAT  >  NC_000913/4212932‑4213203                                          |                                                                                                                                                                                                                                       gTGAGGCTTAACCTTACAACGCCGAAGCTGGTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTTCCAGGCATCAAATTAGAAAAACCCCGGTCCAt                      <  2:369345/251‑1 (MQ=255)         taaaattACAACGCCGCAGCAGTTTTGGCGGAT‑GCGAGAAGATTTTCAGCCTGATACAGATTAAATAAGTACGCAGACGCGGGCTGATAAAACAGAATTTGCCTGGCGGAAGTAGCACGGTGGACCCAACTGACCCCATGCCGAACTCAGAAGAGAAACGCCGTAGCGCCGATGGGCGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCACATTAGAAAATCCCCGGTCCATAAGGCCgg              >  2:377548/6‑251 (MQ=11)         tAACCTTACAACGCTGAAGCTGGTTTGGCGGAG‑GAGAGAAGTTTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGGCTGATAAAACAGAATTTGCCTGGCTGCAGTAGCGCGGTGGGCCCCCCTGACCCCATGCCGAACTCAGTAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTTCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAATCCCCGGTCCATAAGGCCg               <  2:485857/250‑1 (MQ=11)               tACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCGGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGTTCCATAAGGCCGGGGtt          <  2:398321/249‑1 (MQ=255)                  aaCGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTg     >  1:50876/1‑251 (MQ=255)                     gccgacgctgtTTTGGCGGAT‑GAGAGAAGCTTTTCAGCCTGATACAGATTAACTCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGcct  >  2:487794/9‑249 (MQ=255)                                          |                                                                                                                                                                                                                                       GTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCAT  >  NC_000913/4212932‑4213203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑