Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,207,5490G.97.2% 302.4 / 0.8 71coding (179/606 nt)ttdBL(+)‑tartrate dehydratase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base . (35/34);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

CGTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTG  >  NC_000913/3207303‑3207793
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    
cgtcgCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGcccc                                                                                                                                                                                                                                                   >  1:70102/1‑250 (MQ=255)
   cgcgGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTCTGAAAAAGATCCTGACAACCCAGCTCAAAG‑CTGAAGCTCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCg                                                                                                                                                                                                                                              >  1:69345/1‑251 (MQ=255)
    gcgccccGCTGCTGGTACAGGCCTATCTCACCTTTGAAAACCTGTCACACTCCCGGAGCGCGTTATGAAAAAGATACTGACAACCCCGATCAAAG‑CTGAAGTTCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGCCCGGTACGTTGGTGACCTGCCGCGACTTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGt                                                                                                                                                                                                                                             <  2:162091/245‑1 (MQ=255)
       gCACGGTGCTGGTTCATCCCGATCTCACATTTGAAAATCTGTCTCCCACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAACG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACAGGTCCGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGTTCGACCTGAAACGTCCGATCACTTACGATCTCAACGGCACAGCGATTTTCCACGCTG‑CCCCATCGTgcg                                                                                                                                                                                                                                          >  2:230201/1‑251 (MQ=255)
         aCGCTGATGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACATGACAGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTACAAGCTG‑CCCCATCGTgagc                                                                                                                                                                                                                                         >  2:449184/1‑247 (MQ=255)
                 ggTTCATTCCGATCTCCCCTTTGAAAACCTGTCTCACCCCCGGAGCGCGTTATGAAAAAGATCCTGACAGCCCCGATCAAAT‑CTGAAGACTTGAAAGATATTCGCGCCGTCGATGTGATCTACCTGCCCGGTCCGCTGGTGACCGGCCGCGACGGTTGTCACCGCCGTTTGATCTAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGc                                                                                                                                                                                                                                <  2:306052/251‑1 (MQ=255)
                 ggTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAACGATCCTGACAACCCCGATCAAAG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCCAAAACGGc                                                                                                                                                                                                                                >  2:136074/1‑251 (MQ=255)
                 ggTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTCCCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACgg                                                                                                                                                                                                                                 <  1:141050/250‑1 (MQ=255)
                                ccacttgaaaaaTCGGTCTCACACCCGGAGCGCGTTATTAAAAAGATCCTGCCAACCCCGACCAAAG‑CGGAAGATCTGCAAGATATCCGCGCCGGCGATGGGATCTACCTGACCGGTACGCGGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATg                                                                                                                                                                                                                        <  2:154959/236‑1 (MQ=255)
                                 aCCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATgg                                                                                                                                                                                                                <  1:437983/251‑1 (MQ=255)
                                        aaaaTCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGCTCAAAG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGCTGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAACCGGCGACAAATGGGAGATGGTCTCCGt                                                                                                                                                                                                         >  1:508431/1‑251 (MQ=255)
                                        aaaaTCTGTCCCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGGACGCTGGTGCCCGGCCGCGCCGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATTGGAGATGGTCTCCGt                                                                                                                                                                                                         <  2:338082/251‑1 (MQ=255)
                                         aaacaggtcccacaCCCGGAGCGAGTTATGAAAAAGTTCCTGACACCCACGAGCAAAG‑CTAACGTCCTGAATGATTTTGCCGGCGCCGATGTGTTCTACTGGACCGTTACGCTGGTTCCCTGCCGCGACGTTTGTCACCGCCGTGTGATCGAACTGAAACCGCCGATCCCTTACGATCTCAACGGCAAAGCGATTGTCCACGCTG‑CCCCATCGTGCGCAAAATCGGCGACAAATGGGAGATGGTCTCCGTc                                                                                                                                                                                                        <  2:182408/241‑1 (MQ=255)
                                             cTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGcc                                                                                                                                                                                                    >  1:116391/1‑251 (MQ=255)
                                                   cacaCCCGGAGGCCGTTATGAAAAAGATCCTGCCAACCCCGA‑CAAAGGCTGAAGATATGCAAAAAATTCGGGTCGGAGATGTGATCTCCCTGACCGGTACGCTGTGGACCTGCCGCGACGTTTGTCTCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGTTCTCCGTCGGCCCGACa                                                                                                                                                                                               <  2:432113/250‑1 (MQ=255)
                                                   cacaCCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACa                                                                                                                                                                                               >  1:47855/1‑250 (MQ=255)
                                                   aacacCAGGACCGCGCTATGAAACAGAACATGACAACCCCGCTCAAAG‑CTGCAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGATGGTGACCTGCCGCGCCGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTACGCAAAAACGGCGACAAATGGGCGCTGGTCTCCGTCGGCCCgcca                                                                                                                                                                                               >  2:336607/2‑247 (MQ=255)
                                                     cacCCGGAGCCCGTTATGAAAAAGATCCTGACACCCCCGATCAAAG‑CTGCAGATCTGCAAGCTATTCGCGTCGGCGATGTGCTCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑ACCCATGGTGCGCAAAAGCGGAGACAAATGGGAGATGGTCTCCGTCGGCCCGAcccac                                                                                                                                                                                            >  2:78761/1‑247 (MQ=255)
                                                       cccGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGGGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAg                                                                                                                                                                                          <  1:348504/251‑1 (MQ=255)
                                                               gtgttagtaaaaaaATCCTGCCACCCCCGACCAAAT‑CGGAAGCTCTCCAAGAAATTCGGGTCGGGGTTGTGATCTACCGGACCGGTCCGCGGGTGACCCGCCGCGACGTTTGTCCCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTGACGATCTCAACGTCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGTCAAATGGGAGATGGTCTCCGTCTGCCCGATAACCAGCATGctta                                                                                                                                                                                  <  2:297728/243‑4 (MQ=255)
                                                                 ggtATGAAAAAACTCCTGACACCCCCGATCAAAG‑CTGAAGATCGGCAAGATATGCGCGCCGGCGATTTGTTCTCCCTGACCGTTACGCGGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGTGAGATGGTCTCCGTCGGTCCGACAACCAGTATGCGTATg                                                                                                                                                                                <  2:108307/249‑1 (MQ=255)
                                                                 gTTATGAAAAAGATCCTGACAACCCCGATCAAAG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGtct                                                                                                                                                                                                             <  1:75252/222‑1 (MQ=255)
                                                                 gTTATGAAAAAGATACTGACAACCCCGATCAAAG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGtct                                                                                                                                                                                                             >  2:75252/1‑222 (MQ=255)
                                                                   tATGAAAAAGATCCTGACACCCCCGATCAAAG‑CTGAAGATCGGCAAGATATGCGCTCCGGCGATGTGATCTACCTGCCCGTGACGCTGGTGCCCGGCCGCGACGTTTGTCACCGCCGTTTGATCTAACTGAAACGGCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATgg                                                                                                                                                                               <  2:462333/250‑1 (MQ=255)
                                                                         aaaGATCCTGACAACCCCGATCAAAG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGttt                                                                                                                                                                        >  1:220194/1‑251 (MQ=255)
                                                                         aaaGATCCTGACAACCCCGATCAAAG‑CTGAAGATCTGCAAGATATTCGCGACGGCGATGTGATCTAACTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGttt                                                                                                                                                                        >  1:490594/1‑251 (MQ=255)
                                                                           aGATCCTGACATCCCCGATAAAAG‑CTAAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCAGGTGACATGCCGAGACGTTTGTCACAGACGTTTGATCGAACTGAAACGTACGATCCCTTTCGATCACAACGGCAAAGCGATTTTCCACGC‑GGCCCCCTAGTGCGCAAAACCGGCAGCAAATGGGAGATGGTCTCCGTCTGCCCTGCAACCaagataagattggaaagact                                                                                                                                                                        >  2:182431/1‑230 (MQ=255)
                                                                             aTCCTGACAACCCCGATCAAAG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGGGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGaa                                                                                                                                                                    <  1:460270/251‑1 (MQ=255)
                                                                                             tCAAAG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGTTCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGCTTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGTGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGGGAATTGATTGAGc                                                                                                                                                    >  1:103262/1‑251 (MQ=255)
                                                                                             tCAAAG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGc                                                                                                                                                    >  1:38238/1‑251 (MQ=255)
                                                                                               aaaG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTGGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTAGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAg                                                                                                                                                  <  1:75135/251‑1 (MQ=255)
                                                                                                 aG‑CTGAAGAGCTGCAAGATATTCGCGTCGGCAATGTGTTCTACCTGACCGGTACGCTGGTGCCCTGCCGCGACGGTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGAGTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGAc                                                                                                                                                <  1:87167/251‑1 (MQ=255)
                                                                                                    cTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCg                                                                                                                                              >  1:284008/1‑251 (MQ=255)
                                                                                                              gcgaaaactccgcgTCGTCGATGTTATCTACCTTACCGGTGCGCTGGTGACCGTCCGTGAGTTTTGTCTCCGCCGGTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACt                                                                                                                                    <  2:156749/241‑1 (MQ=255)
                                                                                                              gCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGATG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACt                                                                                                                                    >  1:88142/1‑251 (MQ=255)
                                                                                                               cAAGATATTCGCGACGGCGATGTGATCTCCCTGACCGGTACGCAGGTGACCTGACGCGACGCTTGTCACCGCAGTTTGATCGAACTGAAACGTCCGCTCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGCATGGACAGATTTGAACGTGGATTTATTGCGCAGACCGGCGTGAAACtg                                                                                                                                   >  2:405537/1‑251 (MQ=255)
                                                                                                                aaGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGAATGCGTCTGGCAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACtgg                                                                                                                                  >  2:119083/1‑251 (MQ=255)
                                                                                                                       tCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGTCCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATTGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTg                                                                                                                            <  1:431927/250‑1 (MQ=255)
                                                                                                                        cgcgTCGGCGAGGTGTTCTACCTGCCCGGTACGCGGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGc                                                                                                                          <  2:198139/251‑1 (MQ=255)
                                                                                                                                     tgATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGCCAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGGTGGCAAAGGTGGTATgg                                                                                                             >  2:263006/1‑251 (MQ=255)
                                                                                                                                           tccTGGCCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCTATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGTAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATgggg                                                                                                           <  1:41191/246‑1 (MQ=255)
                                                                                                                                            ccTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCt                                                                                                      <  1:411543/251‑1 (MQ=255)
                                                                                                                                               gACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATGTATTGAGCAGACCGGCGTGAAACTGGAGGTTGGCAAAGGTGGTATGGGGCCGCTGAc                                                                                                   >  1:493324/1‑251 (MQ=255)
                                                                                                                                                   ggaccGCTGGTGACCTGCCGCGCCTTTTGTCCCCTCCGGTTGATCGAACTGAATCGTCCGATCCCTTAAGATCGCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTTCGTCGGCCCGACAACCAGTATGCTTATGGAAAGTTTTGAGCGTGAATTTATTGAGCAGACCGTCGTGAAACTGGTGTTTTTCAAAGGTGGTATGGTGCCGATGACCgaa                                                                                               <  2:490594/247‑1 (MQ=255)
                                                                                                                                                     tACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGACCTGAACCGTCCGATCCCTTACGATCTCAACGGCAAAGAGATCTTCCCCGCTG‑CCACATCGTGCGCACAAACCGCGACAAATGGAAAATGGTCTCCGTCGGCCCGACAACCCGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGCGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGATGTATGGGGCCGCTGACCgc                                                                                                >  1:304184/1‑247 (MQ=255)
                                                                                                                                                        gCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGGTGGCAAAGGTGGTATGGGGCCGCTGACCgaagacgg                                                                                          >  1:17364/1‑248 (MQ=255)
                                                                                                                                                                ccTGCCGCGACGTTTGTCACCGCCGTTTTATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCACGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGa                                                                                  <  1:48155/251‑1 (MQ=255)
                                                                                                                                                                  tgacgcgACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAACGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACAGGTGGTTGGCAAAGGAGGTATGGGGCCGCTGACCGACGAAGGCTGcctaaa                                                                                 >  2:200357/4‑246 (MQ=255)
                                                                                                                                                                     cgcgACGTATGTCCCCGCCGTTCAATAGAACTGAAACCCACGATCGCTTCCGACCACCCCGGCAACGCGATTTCCCACCCTG‑ACCCATCGTGCGCAAAACCGGCTAACAACGGGCGATGCTCTCCGTCGGCCCGACAACAAATCTGACTCTGGCAAGCTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTTGCAAAGGAGGATTGGGGCCGCTGACAGAAGAAGGGTGCCGGaaaatc                                                                             >  2:330729/1‑248 (MQ=255)
                                                                                                                                                                          cGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGgc                                                                        <  1:373012/251‑1 (MQ=255)
                                                                                                                                                                          cGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGgc                                                                        <  1:450240/251‑1 (MQ=255)
                                                                                                                                                                          cGTTTGCCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGgc                                                                        <  2:70102/251‑1 (MQ=255)
                                                                                                                                                                                  cccgccgTTTGATCGAACTGAAACGGACGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGGTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAAGTCAAGGCGCTACAtg                                                                <  2:60576/250‑1 (MQ=255)
                                                                                                                                                                                        gTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGAttt                                                          >  1:128770/1‑251 (MQ=255)
                                                                                                                                                                                         tttGATGGAAATGAAACGTCCGATCTCTTACGATCTCAACGGACAAGCAATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGCTGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGATTTTATTGAGCAGACCGGCGTGAAACTGGCGGATGACAGAGGTGGTATGGGGCCGCTTCGCGAAGAAGTCTGCCAGAAATTCAAGGCGCTACATGAGAtttt                                                         >  2:84676/1‑251 (MQ=255)
                                                                                                                                                                                            gATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTccc                                                      >  1:72973/1‑251 (MQ=255)
                                                                                                                                                                                                   cTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCa                                                                                                                                                                                           >  2:410269/1‑111 (MQ=255)
                                                                                                                                                                                                   cTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAAGGGGAGATGGTCTCCGTCGGCCCGACAACCa                                                                                                                                                                                           <  1:410269/111‑1 (MQ=255)
                                                                                                                                                                                                     gAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTg                                             >  1:120040/1‑251 (MQ=255)
                                                                                                                                                                                                        aCGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTgcgc                                          >  1:12948/1‑251 (MQ=255)
                                                                                                                                                                                                               aTCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTCCATGTGATTTTCCCGGCAGGCTGCGCGGTGCTg                                   >  1:104028/1‑251 (MQ=255)
                                                                                                                                                                                                                    ttACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTggcggc                              >  1:441721/1‑251 (MQ=255)
                                                                                                                                                                                                                     tACGATCTCAACGGCAAAGCGATGTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATAGGAGAGGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGGTTTGAACGTGTATTTATTGAGCAGGCCGGCGTGAAACGGTTTGTTGGAAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCATAAATTCAAGGCGCTACATGTGAGTTTCCCGGCAGGTTGCGCGGTGATagcggc                              <  2:411322/250‑1 (MQ=255)
                                                                                                                                                                                                                             cAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGTGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAg                       <  1:340327/249‑1 (MQ=255)
                                                                                                                                                                                                                             cAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGTTGCTGTCTGCAAccc                         <  1:433021/247‑1 (MQ=255)
                                                                                                                                                                                                                             cAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGATGCGCGGTGCTGGCGGCAACCCAGGt                     <  2:441883/251‑1 (MQ=255)
                                                                                                                                                                                                                             cAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTAGGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGTCTGCGCGGTGCTGGCGGCAACCCa                        <  2:116391/248‑1 (MQ=255)
                                                                                                                                                                                                                                   cAAAGCGATTTTCCACGC‑GGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAaga               <  2:284008/251‑1 (MQ=255)
                                                                                                                                                                                                                                     aaGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGGTTTGAACGTGAATTTATTGAGCAGAGCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCGGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAgaga             <  1:336607/251‑1 (MQ=255)
                                                                                                                                                                                                                                       gCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGAtt           >  1:25403/1‑251 (MQ=255)
                                                                                                                                                                                                                                               ccACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGCAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGt   >  1:457761/1‑251 (MQ=255)
                                                                                                                                                                                                                                                cACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCCGGGGGAAGGGATTGAACAAGTg  >  2:369609/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    
CGTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAG‑CTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTG  >  NC_000913/3207303‑3207793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑